ANALYTICAL BIOCHEMISTRY
·
2018
·
IF 2.275
Xiao-Ling Tang,Hui Suo,Ren-Chao Zheng,Yu-Guo Zheng
Tyrosine phenol-lyase (TPL) naturally catalyzes the reversible β-elimination of l -tyrosine to phenol, pyruvate and ammonium. With its reverse reaction (synthetic activity), l -tyrosine and its derivatives could be synthesized with high atom economy, which are widely used in pharmaceutical industries . In this study, a high-throughput screening method for synthetic activity of TPL was developed. One of the substrate, sodium pyruvate was found to react with salicylaldehyde under alkali condition, forming a yellow color compound. The concentration of sodium pyruvate can be quantified according to the absorbance of the colorimetric compound at wavelength of 465 nm and the activity of TPL could be screened according to the decrease of the absorbance. After optimization of the colorimetric reaction conditions, the established high-throughput screening method was successfully used for screening of TPL with enhanced activity for l -DOPA synthesis. The confirmed sensitivity and accuracy demonstrated the feasibility and application potential of this screening method.
研究领域:
生物化学
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制药工程
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DOI
PubMed
高影响因子
ChemPlusChem
·
2018
·Vol. 83
·期 11
·1038-1043
·
IF 3.205
Song Wang,Hejin Jiang,Li Zhang,Jian Jiang,Minghua Liu
Graphical Supramolecular gels formed from hemin and histidine amphiphiles were tested as artificial enzymes to catalyse the oxidation of the chiral substrate L-DOPA to L-dopachrome. The supramolecular gel made up of left-handed helices enhanced the catalytic activity of hemin effectively, and more interestingly, it exhibited enantioselectivity. Supramolecular co-assembly provides an efficient way to combine structural and functional units through noncovalent interactions and produce materials with enhanced performance. Here, using a chiral amphiphilic histidine derivative (LHC18 or DHC18) as a gelator, we have successfully co-assembled the gelator and hemin through gelation. During the co-assembly, the chiral information of the gelator was transcribed into the assemblies, and thus right- and left-handed helical ribbons from LHC18-Hemin and DHC18-Hemin co-assemblies were obtained, respectively, which were confirmed by CD spectra and SEM observations. These helical ribbons were used as artificial enzymes to catalyse the oxidation reaction of 3,4-dihydroxy-L-phenylalanine (L-DOPA). The catalytic activity of hemin in supramolecular gels can be effectively enhanced over that of free hemin. Moreover, by virtue of induced chirality, the supramolecular gels of LHC18-Hemin and DHC18-Hemin exhibited obvious enantioselective activity. This work provides a way to combine chirality transfer and supramolecular asymmetric catalysis.
研究领域:
生物化学
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材料科学
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DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 4
·1387-1394
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IF 1.938
Lu Lin,Hui-Ting Lian,Xiang-Ying Sun,Ya-Ming Yu,Bin Liu
A molecularly imprinted polymer (MIP) based sensor for detecting L-dopa was fabricated based on the composite of graphene (GR) and chitosan (CS). The microstructure and composition of the imprinted films characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR), Raman spectroscopy and electrochemical impedance spectroscopy (EIS) indicated that the modified film of GR-MIPs had been formed successfully. Electrochemical evaluation results showed that the sensor exhibited a satisfactory selectivity for molecular binding with an imprinting factor (IF) of 6.2 and a wide linear range from 0.4 μM to 100 μM with a low detection limit of 0.012 μM. This electrochemical sensor exhibited great promise for accurate determination of L-dopa in pharmaceutical preparations and human blood serum for recoveries ranging from 95.0 to 108.0%. Furthermore, compared to imprinted films without graphene doping, GR-MIPs showed a superior chiral sensing for L-dopa in the presence of D-dopa. The results reveal that the insertion of graphene brought about an enhanced sensitivity for chiral sensing and has the potential for application in chiral recognition.
研究领域:
分析化学
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材料科学
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生物医学工程
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DOI
高影响因子
Science China-Chemistry
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2020
·Vol. 63
·期 9
·1295-1305
·
IF 6.356
Wang Xianheng,Yang Lei,Yang Peng,Guo Wancai,Zhang Quan-Ping,Liu Xianhu,Li Yiwen
Melanin-inspired polymers are currently the focus of growing interest for a wide range of applications ranging from energy to biomedical area. Whilst researchers have made numerous attempts to prepare and utilize polydopamine nanoparticles (PDA NPs), they have made limited progress in developing and discovering another typical functional mimic of natural melanin, poly (levodopa) (P(L-DOPA)) NPs, probably due to the lack of facile synthetic strategies towards satisfactory structural and functional control of melanin-like NPs. Herein, we reported a one-pot preparation method towards P(L-DOPA) NPs with good yields and controllable size/property in an aqueous solution assisted by various metal ions ( i.e. , Ni(II), Mg(II), Ca(II), Fe(III), Mn(II), Co (II), Zn(II) and Cd(II)). Interestingly, the resulting P(L-DOPA) NPs exhibited enhanced light absorption and photothermal behaviors compared with well-established PDA NPs, which can be employed to further fabricate kinds of photothermal composite actuators with promising performances such as folding, switching, and forward-moving. This study offers a facile and robust way to synthesize new synthetic melanins beyond PDA, and facilitates further functional discovery and evolution of melanin-inspired polymers and composites.
研究领域:
材料科学
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生物医学工程
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高分子化学
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DOI
高影响因子
CHEMICAL ENGINEERING RESEARCH & DESIGN
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2023
·
IF 3.9
Nor Hafiza Ismail,Wan Norharyati Wan Salleh,Farhana Aziz,Norhaniza Yusof,Juhana Jaafar,Ahmad Fauzi Ismail,Ahmad Hafizal Mohd Yamin
Oily wastewater poses a significant environmental concern as it has adverse effects on both ecosystems and human health. The development of highly efficient and robust antifouling membranes for oily emulsion is in great demand to prevent these serious issues in oil/water emulsion filtration. Moreover, fouling was inevitable when dealing with membrane separation . In order to overcome the addressed matters, this paper reported sprayed hydrophilic surface coating using commercialized levodopa ( L -DOPA). The coated solution was mixed with 3-amino-propyltriethoxysilane in various ratios to obtain a smooth coated layer. Based on the results, the highest pure water flux and rejection efficiency of 586.51 ± 23.95 L/m 2 ‧h and 98.22% was achieved in this study, respectively. The abundant hydroxyl and carboxyl functional groups on the membrane surface from L -DOPA benefit the membrane in many ways, especially the membrane wetting properties. The water and oil contact angles of the coated membrane increased by approximately 75% and 85%, respectively. The fouling behaviors are influenced by the presence of the coated layer, resulting in the attainment of 90% fouling resistance ratio (FRR). This is mostly due to the reduction in adherence of oil droplets on the membrane surface, hence enhancing the effectiveness of the anti-oil fouling membrane . Additionally, this provides excellent properties of the membrane to be applied in oily wastewater treatment .
研究领域:
环境科学
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材料科学
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化学工程
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Haichao Zhao,Tian-Yu Sun,Liang-Feng Huang,Jiayu Wei,Shihui Qiu
Carbon dots as a specific type of carbon material require the connection of experiment to theory, exploration of new application, and development of new material. Here we present a new nitrogen-doped polymer nanodots (N-PDs) from 3,4-dihydroxy-L-phenylalanine (L-DOPA) via the one-step synthesis of simultaneous self-polymerization and carbonization process. This water-dispersible N-PDs, composed of poly-L-DOPA aggregates and embedded nitrogen-doped graphene patches, shows the zero-dimensional structure with an average diameter of 2.06 nm. In-depth investigation of the inhibition effect of as-prepared N-PDs demonstrates that N-PDs can suppress the oxygen and chloride corrosion of mild steel in extremely acidic condition through the dual effect of adsorption/assembly onto metal surface as a passivation film and strong covalent bonding with iron, resulting in an inhibition efficiency of 97.95% at the concentration higher than 200 mg/L within 6 h, the most efficient reported for carbon dots-based corrosion inhibitors. Furthermore, density-functional-theory calculations clearly reveal that the atoms present in unsaturated bonds of N-PDs play a dominant role in strengthening the interaction between corrosion inhibitor and metal substrate, which gives rise to the stable and homogeneous adsorption of N-PDs and then the effective protection for metal substrate against corrosion.
研究领域:
材料科学
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腐蚀与防护
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理论计算
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DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
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2018
·Vol. 36
·期 11
·1251-1261
·
IF 2.016
Zhang Pei-Bin,Tang An-Qi,Wang Zhang-Hui,Lu Jing-Yu,Zhu Bao-Ku,Zhu Li-Ping
Developing a low-cost and well-recyclable adsorbent with high adsorption capacity is greatly desirable in dye wastewater treatment. Here, we demonstrate a kind of novel tough and reusable hydrogel beads with quite high capacity of dye adsorption via incorporating mussel-bioinspired poly(L-DOPA) (PDOPA) into alginate/poly(acrylamide) double network (DN) hydrogels. The synthesized PDOPA nanoaggregates were introduced into the DN hydrogels by simple one-pot mixing with the monomers prior to polymerization. The fabricated hydrogel beads exhibited high mechanical strength and good elastic recovery due to the interpenetrating Ca 2+ -alginate and poly(acrylamide) networks. It was shown that the beads exhibited relatively high dye adsorption capacity compared to other adsorbents reported in literature, and the introduction of PDOPA with an appropriate amount raised the adsorption capacity. It is believed that the addition of PDOPA and the matrix of double network architecture contributed synergistically to the high adsorption capacity of hydrogel beads. Moreover, the desorption of dyes could be easily realized via rinsing in acidic water and ethanol solution. The hydrogel beads remained the high adsorption capacity even after 5 times of adsorption and desorption cycles. This tough and stable hydrogel with high adsorption capacity may have potential in treatment of dye wastewater released by textile dyeing industry.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
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2022
·
IF 3.191
Hang-Qin Zhu,Wen-Ye Hu,Xiao-Ling Tang,Ren-Chao Zheng,Yu-Guo Zheng
Tyrosine phenol-lyase (TPL) exhibits great potential in industrial biosynthesis of l -tyrosine and its derivates. To uncover and screen TPLs with excellent catalytic properties, there is unmet demand for development of facile and reliable screening system for TPL. Here we presented a novel assay format for the detection of TPL activity based on catechol 2,3-dioxygenase (C23O)-catalyzed reaction. Catechol released from TPL-catalyzed cleavage of 3,4-dihydroxy- l -phenylalanine ( l -DOPA) was further oxidized by C23O to form 2-hydroxymuconate semialdehyde, which could be readily detected by spectrophotometric measurements at 375 nm. The assay achieved a unique balance between the ease of operation and superiority of analytical performances including linearity, sensitivity and accuracy. In addition, this assay enabled real-time monitoring of TPL activity with high efficiency and reliability. As C23O is highly specific towards catechol, a non-natural product of microorganism, the assay was therefore accessible to both crude cell extracts and the whole-cell system without elaborate purification steps of enzymes , which could greatly expedite discovery and engineering of TPLs. This study provided fundamental principle for high-throughput screening of other enzymes consuming or producing catechol derivatives.
研究领域:
生物化学
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工业生物技术
·
酶工程
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Jiaxue Liang,Hang Qi,Feng Zhu,Shuqi Chen,Bohua Liu,Chongling Sun,Yanyan Wang
Real-time monitoring the drugs penetration into brain is crucial for clinical therapy of neurodegenerative diseases. In this study, a Blood-Brain Barrier-electrochemical sensing (BBB-ES) platform combining BBB on chip model and electrochemical sensing system was proposed, which facilitated stable BBB growth under fluidic conditions and realized real-time detection of L-Dopa across the BBB. Based on this system, the effects of fluid force on the cell morphology, skeleton arrangement and tight junction protein ZO-1 of BBB growth were evaluated, it was verified that the fluid force promote cell rearrangement. Additionally, L-Dopa detecting performance was optimized by modifying SWNTs-AuNPs-PPy/Tyr(Single-Walled Carbon Nanotubes-Au Nanoparticles-Polypyrrole/Tyrosinase) on electrochemical electrode, which showed a wide linear response range of 10–500 μM and excellent selectivity toward L-Dopa. The integrated BBB-ES system was finally applied to real-time monitor the penetration process of L-Dopa through the BBB, it was proved that the penetration of L-Dopa increased with the increase of flow rate and time, and finding that L-Dopa was delivered by passive diffusion without disrupting the integrity of BBB. Based on these findings, the system can be used to monitor the real-time process of different drugs penetrating BBB and even different organ barriers, which also provide the possibility to solve the clinical demand for drugs screen.
研究领域:
神经科学
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生物医学工程
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药学
·
电化学
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产品关联
DOI
高影响因子
Sensing and Bio-Sensing Research
·
2025
·
IF 4.9
Jianjun Kang,Xue Lin,Chen Lin,Wuyuan Pan,Jian Wang,Fang Ke
Levodopa (L-DOPA) is a crucial neurotransmitter utilized in the treatment of neurological disorders, particularly in Parkinson's disease, where its concentration in biological fluids serves as a key diagnostic marker. Despite its significance, current methods for L-DOPA detection often suffer from limitations such as prolonged detection times, low sensitivity and weak anti-interference. This study addresses these challenges by introducing a highly efficient and colorimetric sensor for the rapid detection of L-DOPA, utilizing manganese-doped graphitic carbon nitride (Mn-g-C 3 N 4 ) as a peroxidase-mimetic catalyst. The sensor employs 3,3′,5,5′-tetramethylbenzidine as a substrate and achieves detection within 20 s, with a linear response over the concentration ranges of 1–10 μM and 20–90 μM, and the detection limits were 0.049 μM and 0.51 μM, respectively. The method has good specificity for L-DOPA and has been successfully applied to human serum even in the presence of interference from dopamine and other amino acids. In addition, the sensor exhibits satisfactory performance in smartphone-based L-DOPA detection. The novel sensor platform offers a simple, cost-effective, and scalable solution for real-time L-DOPA monitoring, with significant potential for applications in both clinical and environmental contexts.
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DOI
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Shengwei Jia,Xiaoxia Li,Fang Wang,Hao Zhang,Qun-Dong Shen
Functional nanomaterials show great promise in next-generation targeted non-genetic neuromodulation and on-demand neurotransmitter release with superior spatiotemporal accuracy. L-DOPA-induced dyskinesia (LID) is a common complication during the treatment process of Parkinson's disease. Dysfunction in the dynamics of dopamine release plays a pivotal role in the disease. A strategy for LID treatment by using ultrasound-responsive functional nanomaterials for controlled release of nitric oxide is proposed, which can activate the nitric oxide (NO)-soluble guanylate cyclase (sGC)-cyclic guanosine monophosphate (cGMP) signaling pathway. This alleviates the abnormal synaptic plasticity caused by excessive and pulsatile dopamine, and diminishes hypersensitivity of the D 1 dopamine receptors, thereby reducing the behavioral disorders associated with LID. These findings offer a novel perspective for therapy of neurodegenerative diseases.
研究领域:
纳米材料
·
神经科学
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药理学
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神经退行性疾病
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生物医学工程
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DOI
PubMed
高影响因子
Microbial Biotechnology
·
2022
·Vol. 15
·期 5
·1610-1621
·
IF 6.575
Xing Liu,Xiao Han,Yuan Peng,Chunlin Tan,Jing Wang,Hongsong Xue,Ping Xu,Fei Tao
Summary 3, 4-Dihydroxyphenyl- l -alanine ( l -DOPA) is a compound of high medical value and is considered effective as a treatment for Parkinson’s disease. Currently, bioproduction of l -DOPA is mainly carried out by whole-cell catalysis mediated by recombinant Escherichia coli carrying heterogeneous tyrosine phenol lyase. Vibrio natriegens is increasingly attracting attention owing to its superiority, including extremely rapid growth and high soluble protein expression capacity. In this study, we attempt to develop an efficient whole-cell catalyst for l -DOPA production using V. natriegens as the chassis. The maximum soluble protein expression by V. natriegens was accomplished in 4 h at 37°C, which was equivalent to that achieved by E. coli in 16 h at 16°C. Furthermore, the maximum productivity reached over 10.0 g l −1 h −1 in the early stage of biocatalysis, nearly two-fold higher than previously reported. Approximately 54.0 g l −1 l -DOPA was obtained with a catechol conversion rate greater than 95%. In conclusion, V. natriegens displays advantages, including rapid protein expression and catalytic rate in the catalysis process for l -DOPA production. These findings strongly suggest that V. natriegens has remarkable potential as a whole-cell catalysis chassis for the production of valuable chemicals.
研究领域:
生物工程
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医学
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微生物学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）