INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Maosheng He,Ying Yan,Xiang Liu,Linhan Li,Bin Yang,Mingzhu Liu,Qing Yu,Erlong Wang,Pengfei Li,Tianqiang Liu,Gaoxue Wang
Diseases caused by pathogens severely hampered the development of aquaculture, especially largemouth bass virus (LMBV) has caused massive mortality and severe economic losses to the culture of largemouth bass ( Micropterus salmoides ). Considering the environmental hazards and human health, effective and environmentally friendly therapy strategy against LMBV is of vital importance and in pressing need. In the present study, a novel nanobody (NbE4) specific for LMBV was selected from a phage display nanobody library. Immunofluorescence and indirect ELISA showed that NbE4 could recognize LMBV virions and had strong binding capacity, but RT-qPCR evidenced that NBE4 did not render the virus uninfectious. Besides, antiviral drug ribavirin was used to construct a targeted drug system delivered by bacterial nanocellulose (BNC). RT-qPCR revealed that NbE4 could significantly enhance the antiviral activity of ribavirin in vitro and in vivo . The targeted drug delivery system (BNC-Ribavirin-NbE4, BRN) reduced the inflammatory response caused by LMBV infection and improved survival rate (BRN-L, 33.3 %; BRN-M, 46.7 %; BRN-H, 56.7 %)compared with control group (13.3 %), ribavirin group (RBV, 26.7 %) and BNC-ribavirin (BNC-R, 40.0 %), respectively. This research provided an effective antiviral strategy that improved the drug therapeutic effect and thus reduced the dosage.
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PubMed
高影响因子
AQUACULTURE
·
2024
·
IF 3.9
Jingya Li,Mingyang Xue,Chen Xu,Nan Jiang,Yiqun Li,Yuding Fan,Yong Zhou,Yan Meng
Largemouth bass ranavirus (LMBRaV) is a highly lethal virus that has devastated the largemouth bass aquaculture sector. Therefore, it is imperative to develop therapeutic strategies for the control of this pathogen. In this work, the antiviral activity of Saikosaponin C was examined both in vitro and in vivo . According to the results of the in vitro antiviral activity assay, 30 mg/L of Saikosaponin C significantly down-regulated the expression of the LMBRaV major coat protein (MCP) gene in epithelioma papilloma cyprinid (EPC) cells, with an inhibitory rate exceeding 84%, and prevented LMBRaV replication in a dose-dependent manner. It also significantly limited its cytopathic effect (CPE), thereby playing a protective role on infected cells. Notably, Saikosaponin C has a certain preventative impact on the virus by influencing the mechanisms by which viral particles connect to cell receptors, which prevents LMBRaV from adsorbing on EPC cells. Simultaneously, Saikosaponin C also directly inactivated LMBRaV. The results of exploring the viral invasion cycle demonstrated that Saikosaponin C primarily exerted its antiviral effect during the middle stage (2–6 h) of viral infection. In addition, Saikosaponin C inhibited LMBRaV-induced apoptosis of EPC cells, maintained physiological cellular morphology and structure, and protected cells from loss of cellular morphology, formation of apoptotic vesicles, and cell fragmentation. Moreover, Saikosaponin C considerably improved the survival of virus-infected largemouth bass, according to the findings of in vivo investigations. In summary, Saikosaponin C demonstrated excellent anti-LMBRaV activity and holds considerable promise for development in the field of aquaculture.
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高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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2026
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IF 4.4
Xiaofei Yang,Mingyue Qiao,Yan Deng,Songsong Chen,Cheng Li,Yuanke Shu,Li Jiang
Heavy metal pollution, particularly copper (Cu) and cadmium (Cd), poses a serious threat to aquatic ecosystems due to its toxicity and bioaccumulation potential. This study investigated the effects of individual and combined exposure to Cu and Cd on largemouth bass, focusing on the gut–liver axis and the TLR4/NF-κB pathway in hepatotoxicity. A total of 480 size-matched fish were randomly assigned to four groups (Control, Cu, Cd, and Cu + Cd) and exposed to sublethal concentrations of CuSO₄ (9.275 mg/L) and CdCl₂·2.5H₂O (1.15285 mg/L) for 14 days, followed by physiological, molecular, and omics analyses. Results showed that both single and combined exposures disrupted intestinal structure and barrier function, accompanied by downregulation of tight junction genes. Oxidative stress responses were tissue-specific, with increased reactive oxygen species (ROS) and MDA levels in the intestine but decreased levels in the liver, along with suppressed antioxidant enzyme activities. Inflammatory responses were activated, as indicated by elevated cytokine levels and upregulation of TLR4/MyD88/NF-κB signaling in the liver. Microbiota analysis revealed that Cd exposure increased gut microbial diversity, whereas Cu + Cd co-exposure reduced α-diversity and altered microbial composition, with increased Proteobacteria and decreased beneficial taxa such as Bacteroidota and Firmicutes. Untargeted metabolomics showed that hepatic metabolic profiles were altered, mainly affecting lipid, energy, and immune-related pathways. Overall, combined Cu + Cd exposure exerted stronger toxic effects than single exposures, inducing more severe intestinal damage, microbial dysbiosis, and metabolic disturbances. These findings highlight the critical role of the gut–liver axis in mediating heavy metal toxicity.
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PubMed
高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
·
2026
·
IF 4.3
Zhang Zhenyang,Chen Weijun,Shen Jiaqi,Dong Haowei,Zhang Chunnuan,Gao Xiaochan,Ren Hongtao,Huang Yong,Gao Shiyang
Waterborne cadmium (Cd) remains a significant threat to the intestinal health and growth of largemouth bass ( Micropterus salmoides ), necessitating the search for effective dietary mitigants. In this 56-day trial, the protective efficacy of paeonol (PAE) was evaluated across five experimental groups: a negative control, a Cd-stressed group (Cd, 2.75 mg/L waterborne exposure), and three treatment groups concurrently exposed to 2.75 mg/L Cd and supplemented with dietary PAE at 100, 200, and 400 mg/kg, respectively. The results showed that Cd exposure induced intestinal oxidative stress, impaired structural integrity, downregulated antimicrobial peptide expression, compromised immune function, and disrupted intestinal microbial homeostasis ( P < 0.05). PAE supplementation significantly decreased malondialdehyde (MDA) content, and the PAE200-Cd exhibited increased activities of total superoxide dismutase (T-SOD) and catalase (CAT), as well as elevated expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and cytochrome P450 1A1 (CYP1A1) ( P < 0.05). Intestinal villus structure was restored, the number of goblet cells increased, and the expression of tight junction proteins (Occludin, ZO-1, Claudin-1) genes was significantly upregulated ( P < 0.05). Meanwhile, the expression of β-defensin, Lysozyme, and Mucin 2 was significantly upregulated ( P < 0.05). The PAE100-Cd showed increased expression of immunoglobulins (e.g., IgM and IgT), while the PAE200-Cd inhibited the expression of pro-inflammatory factors ( P < 0.05). Cd-induced negative changes in intestinal microbial diversity and stability were restored by PAE, and the PAE200-Cd exhibited decreased abundances of Proteobacteria and Pseudomonas , and increased abundance of Firmicutes ( P < 0.05). Under the present experimental conditions, the appropriate dietary PAE level based on oxidative stress and intestinal physical barrier was preliminarily estimated to be 115.51–229.55 mg/kg. Collectively, PAE can alleviate Cd-induced intestinal injury by improving antioxidant capacity and enhancing intestinal barrier function, and represents a promising functional candidate for heavy metal mitigation. Graphical The alternative text for this image may have been generated using AI.
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高影响因子
AQUACULTURE NUTRITION
·
2021
·Vol. 27
·期 6
·2749-2758
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IF 3.497
Yan-Yu Zhang,Jun-Xian Wang,Jiong Ren,Jie Wang,Fang Qiao,Mei-Ling Zhang,Zhen-Yu Du
In aquaculture, excess lipid deposition in liver is a health damage in largemouth bass ( Micropterus salmoides ). Activation of peroxisome proliferator-activated receptor-α (PPARα) has been proved to stimulate lipid catabolism and alleviate fatty liver in rodents and human. Our present study was conducted to investigate the effects of Pparα activation via dietary fenofibrate stimulation on hepatic lipid catabolism in largemouth bass. Total 240 fish (10.7 ± 0.2 g) were randomly divided into eight tanks and fed two diets (control and control containing 5 g/kg fenofibrate), respectively, for 4 weeks. Dietary fenofibrate increased Pparα protein and mitochondrial DNA amounts. The weight gain rate and carcass ratio of fish with Pparα activation decreased, but viscerosomatic index and hepatosomatic index were higher in the fenofibrate-fed fish than the control. Pparα activation significantly reduced triglyceride levels in the serum and liver but did not change the lipid composition in liver. Moreover, hepatic glycogen accumulation was found in the fish with Pparα activation. The Pparα activation also had health benefits for liver, as reflected by reducing serum ALT and AST activities, and increasing antioxidative ability in liver. Our results showed that in largemouth bass, Pparα can be activated and the Pparα activation could efficiently stimulate lipid catabolism and reduce hepatic fat deposition, and promote health in liver.
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高影响因子
AQUACULTURE
·
2023
·
IF 4.5
Xiangjie Mao,Wangwang Chen,Xianmei Long,Xiaomei Pan,Guoqing Liu,Wenguang Hu,Dianchao Gu,Qingsong Tan
The aim of this study was to investigate the effects of dietary iron (Fe) levels on the growth performance, health status, meat quality, and intestinal flora of juvenile largemouth bass ( Micropterus salmoides ) and to determine the dietary Fe requirement of largemouth bass . Largemouth bass was fed with 6 isonitrogenous and isocaloric experimental diets containing Fe levels of 42.26, 50.79, 66.61, 80.86, 123.13, and 201.87 mg/kg for 10 weeks. The results showed that both final body weight (FBW) and specific growth rate (SGR) tended to increase and then decrease with increasing dietary Fe level, reaching their highest values of 50.34 g and 3.58%/d at a dietary Fe concentration of 80.86 mg/kg respectively. Feed efficiency (FE) and protein efficiency ratio (PER) increased linearly with increasing dietary Fe level. Total serum protein (TP) and urea nitrogen (UN) decreased linearly with increasing dietary Fe level. Serum superoxide dismutase (SOD) and catalase (CAT) enzyme activities tended to increase and then decrease with increasing dietary Fe level, while serum malondialdehyde (MDA) level tended to decline and then increase. In addition, increased dietary Fe level tended to improve muscle hardness and chewiness , thus improving meat quality. Analysis of the intestinal flora showed that the abundance of microorganisms such as Plesiomonas , Peptostreptococcaceae , and Lactococcus in the genus level increased significantly at dietary Fe concentrations of 80.86 mg/kg and 201.87 mg/kg, indicating that appropriate levels of dietary Fe can increase the diversity of intestinal microorganisms . Optimal dietary Fe levels for juvenile largemouth bass were determined to be between 73.81 and 83.22 mg/kg based on the broken-line regression analyses of SGR, FE, or hemoglobin content.
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高影响因子
JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 8
·2470-2480
·
IF 2.479
Luyun Cai,Jiangli Wan,Xiuxia Li,Jianrong Li
This study examined the effects on conformation and oxidation of myofibrillar protein in largemouth bass by different thawing methods. The conventional thawing, microwave thawing, microwave (MVT) or ultrasound combined with vacuum thawing, microwave or far-infrared thawing (FMT) combined with magnetic nanoparticles were used in this experiment. The physicochemical changes were analyzed by differential scanning calorimetry and dynamic rheology. The protein structure changes were measured by Raman, intrinsic fluorescence, and second-derivative ultraviolet spectrometry. The degree of protein aggregation was evaluated by surface hydrophobicity, particle size, and zeta-potential measurements. Total sulfhydryl content, protein carbonyl content, Ca 2+ -ATPase activity, and SDS-PAGE were used to analyze the degree of protein oxidation. Results showed that MVT and FMT samples had better thermal stability, more stable protein conformation, and a lower degree of protein oxidation. Thus, these two methods would be beneficial to sustain the quality of thawed fillets. Practical Applications In the market circulation, largemouth bass (Micropterus salmoides) need to be frozen. The thawing methods can directly affect the quality of frozen fish, thus causing the changes in the conformation of the myofibrillar protein in fish, and also affecting the degree of protein oxidation. The results showed that the microwave combined with vacuum and the magnetic nanoparticles combined with far-infrared thawing had less effect on myofibrillar protein of fish and were a better thawing method.
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PubMed
高影响因子
AQUACULTURE RESEARCH
·
2023
·
IF 2
Chunjing Cai,Zhe Li,Junhua Lu,Ying Wang,Chen Wang,Pengfei Li,Hui Wang,Wu Meng,Jie Chu
The purpose of this study was to investigate the effects of dietary acetoin on Largemouth Bass (Micropterus salmoides). In 45 d of farming, the experimental diets were supplemented with 0%, 0.3%, 0.6%, and 0.9% of acetoin levels, respectively. Results showed that the activities of lipase (LPS), amylase (AMS), and trypsin in fish fed with acetoin at 0.6% displayed the most obvious improvement compared with the other levels of acetoin (). Furthermore, the incorporation of acetoin at 0.3% and 0.6% in Micropterus salmoides diet significantly enhanced the number of beneficial bacteria (such as Cetobacterium) and reduced the number of harmful bacteria (such as Proteobacteria) in the gut flora of Micropterus salmoides. Meanwhile, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and catalase (CAT) in the liver of the samples treated with acetoin at 0.6% were significantly higher than the control (), while the content of malondialdehyde (MDA) and the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the liver notably decreased with the increase of the addition amount of acetoin (). Furthermore, the contents of IgA, IL-6, and TNF-α and the activity of acid phosphatase (ACP) in the serum of the samples added with acetoin exhibited a concentration-dependent decline compared with the control (). In summary, acetoin had potential feeding attraction effects on Micropterus salmoides, and the most suitable dose of dietary application was 0.6% acetoin, which can effectively improve the physiological characteristics and immune activity of Micropterus salmoides.
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高影响因子
Comparative Biochemistry and Physiology D-Genomics & Proteomics
·
2026
·
IF 2.9
Xiaolu Shen,Xiaoru Chen,Tao Teng,Dongyu Huang,Leimin Zhang,Mingchun Ren,Qunlan Zhou,Xiaodi Xu,Xujun Wang,Lu Zhang,Hualiang Liang
Alkaline inhibited immunity via immune markers and pathways in largemouth bass. • Alkaline impaired osmoregulation via serum ions and gut osmotic genes in largemouth bass. • Alkaline disrupted antioxidant balance and induced tissue injury in largemouth bass. The purpose of this study was to evaluate the harm of high alkalinity to largemouth bass through physiological and biochemical and transcriptome analysis, and to elucidate its potential molecular mechanism, so as to provide theoretical basis for the sustainable development of alkaline aquaculture. The largemouth bass were divided into normal control group (NC) and alkaline stress group (AS). Freshwater was used for the NC group. Na₂CO₃ and NaHCO₃ were added to the water to reach an additional alkalinity of 6 mmol/L for the AS group. Under alkaline stress, a total of 2014 differentially expressed genes were identified, of which 428 genes were up-regulated and 1586 genes were down-regulated; some serum immune-related indicators decreased significantly, and the differential genes in the immune-related pathways (Calcium signaling pathway, Toll-like receptor signaling pathway and Focal adhesion) in the intestinal tissue were down-regulated as a whole; at the same time, intestinal antioxidant-related indicators (CAT, GSH, MDA, T-AOC) were increased significantly, GSH-Px activity was decreased significantly, and genes in antioxidant-related pathways (Peroxisome, Glutathione metabolism) were also up-regulated as a whole. In addition, the concentration of Na + in serum was decreased significantly, the concentration of K + and Cl − were increased significantly, and the serum osmotic pressure showed a downward trend. Alkaline stress also reduced the expression of osmotic pressure regulatory genes ( cldn15b , ca12 , abcc9 , entpd1 ) in the intestine significantly, and enhanced the expression of slc13a1 . In conclusion, alkaline stress could significantly inhibit the immune function and osmotic pressure regulation ability of largemouth bass, disrupt the balance of its antioxidant system, and cause various damages to the health of the body. Download: Download high-res image (210KB) Download: Download full-size image
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高影响因子
AMINO ACIDS
·
2025
·Vol. 57
·期 1
·1-15
·
IF 2.4
Wang Jiteng,Zhang Jingyi,Li Xinyu,Xu Hanying Y.,Yang Yeshun,Zhang Jiankun,Feng Wenping,Chen Qiang,Dong Fen,Han Tao
The main objective of this experiment was to study the metabolism of arginine in juvenile largemouth bass ( Micropterus salmoides ). A total of 300 healthy fish (average weight of 25 ± 0.5 g) were randomly assigned to ten groups. Experimental fish were orally administered or intraperitoneally injected with 0.9% sodium chloride, arginine, arginine-aspartate, citrulline, and glutamate solutions, respectively. They were euthanized at 10, 30, 60, 120, and 240 min after oral administration or intraperitoneal injection, and various tissue samples were subsequently collected for analysis. The results revealed that serum ornithine and citrulline concentrations of largemouth bass were significantly increased by oral administration of arginine or arginine-aspartate ( P < 0.05). Intraperitoneal injection of arginine or arginine-aspartate solution significantly elevated the concentrations of ornithine and citrulline in the serum, liver, kidney, and muscles ( P < 0.05). The concentrations of citrulline, ornithine, and arginine in serum and muscle increased significantly at 4 h after intraperitoneal injection of glutamate ( P < 0.05). Intraperitoneal injection of citrulline significantly increased the concentrations of ornithine and arginine in the serum and muscles ( P < 0.05). The research findings demonstrate that both free and small peptide forms of arginine were rapidly degraded to ornithine due to the high arginase activity in various tissues of largemouth bass. Additionally, the pathway of synthesizing citrulline from glutamate and then arginine from citrulline may exist in largemouth bass, but the exact location of this synthesis process may differ from that found in mammals.
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PubMed
高影响因子
AQUACULTURE
·
2024
·
IF 3.9
Xiaoyu Qi,Hongzhou Xu,Jingfei Long,Chenyang Yan,Kuiquan Pan,Yanzhen Song,Xinxin Jiang,Haixia Liu
Antibiotics have important role in controlling the outbreak of bacterial diseases in fish farming, but long-term use or persistent existence of antibiotics in water can cause adverse effects on aquatic animals. In order to evaluate repairing effect of Astragalus polysaccharides (APS) on damaged intestine of fish under enrofloxacin (ENR) stress, ENR-pretreated (1.0 mg/L, 7 days) largemouth bass were randomly divided into two groups, and subsequently were fed basal diet and diet supplemented with 1.0 g/kg APS for 14 days. Intestine tissue and content were sampled on day 0 (D0), days 7 (D7), days 21 (D21_APS and D21) to detect the intestinal structure, intestinal microbiota structure, oxidative stress, non-specific immune and inflammatory response. Intestinal tissue section showed that the height, width of intestinal villi, and muscular thickness in D21_APS were significantly greater than those on D0, D7, and D21 ( P < 0.05). APS-supplemented diet was significantly increased expression of tight junction protein related genes occludin, claudin-1, ZO-1 ( P < 0.05). APS addition significantly improved the activities of catalase (CAT) and peroxidase (POD) and decreased superoxide dismutase (SOD) activity ( P < 0.05). The activity of CAT and SOD in D21_APS and D0 were similar. The highest activities of acid phosphatase (ACP), lysozyme (LZM) and alkaline phosphatase (AKP) were found in D21_APS group. The expression of proinflammatory factors IL-1β and TNF-α in D21_APS and D21 were significantly down-regulated compared to that of D7, while anti-inflammatory factor IL-10 was up-regulated ( P < 0.05). Compared with D7 group, quinolone resistance gene ( qnrB ) in D21 and D21_APS groups were significantly down-regulated, and in contrast to D21 group, qnr B and qnrS genes in D21_APS group were significantly down-regulated ( P < 0.05). High-throughput 16SrRNA gene sequencing revealed proportion of Cyanobacteria in intestinal microbiota increased from 8.23% to 52.66%, while Firmicutes decreased from 28.54% to 2.03% after ENR treatment. In D21 group, Proteobacteria was notable decrease compared to D0, while Firmicutes increased. In D21_APS, the dominant bacteria were Proteobacteria, Fusobacteriota and Firmicutes, and Acinetobacter , Cetobacterium and Aeromonas were the predominant genera. Cluster analysis indicated that bacterial community structure in D21_APS has a similar level to D0 at phylum level. Feeding APS increased abundance of genes related to styrene, isoleucine and lysine degradation; propanoate, butanoate phenylalanine, and tryptophan metabolism; lipopolysaccharide biosynthesis; bacterial secretion system; cell motility and secretion at KEGG pathway Level 3 compared to the basal diet. Taken together, APS can repair the damage of intestinal structure and intestinal microbiota caused by ENR.
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高影响因子
Frontiers in Marine Science
·
2026
·
IF 3.4
Xuehe Li,Huixuan Xu,Jinjiang Wang,Maozi Lin,Qihui Yang
IntroductionPlant-derived functional additives have gained increasing attention as sustainable alternatives in aquafeed. This study aims to investigate the effects of dietary monolaurin on productive performance, innate immune indicators, antioxidative effects, and gut flora in largemouth bass (Micropterus salmoides).MethodsFish were fed diets containing 0, 500, 800, 1100, and 1400 mg/kg GML for 56 days, with three replicates per treatment.ResultsThe results showed that GML supplementation significantly improved growth performance. Final body weight was higher in the 800 and 1100 mg/kg groups, while weight gain rate and specific growth rate peaked at 800 mg/kg. Whole-body crude lipid content decreased in the 500 and 800 mg/kg groups, whereas crude protein content increased across all GML-supplemented groups. GML also enhanced antioxidant capacity by increasing total antioxidant capacity and catalase and superoxide dismutase activities while reducing malondialdehyde levels. Serum acid phosphatase and alkaline phosphatase activities peaked in the 800 and 1100 mg/kg groups. The expression levels of il6 and tnfa were significantly downregulated in the 800 and 1100 mg/kg groups, while those of tgfb1a and il22 were significantly upregulated. Intestinal microbiota analysis revealed that GML reshaped the gut microbial community. The relative abundances of Aeromonas and Pseudomonas decreased at 800–1400 mg/kg, whereas those of Cetobacterium and Bacteroides increased at 800 mg/kg and 1100 mg/kg. Regression analysis based on final body weight estimated the optimal dietary GML level at 750 mg/kg.DiscussionThese findings indicate that appropriate dietary GML supplementation can promote growth while improving antioxidant status, selected innate immune responses, and intestinal microbial balance in largemouth bass. Thus, GML may serve as a promising plant-derived functional feed additive in largemouth bass aquaculture.
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高影响因子
危险品化学品经营许可证（带存储）