ACS Applied Nano Materials
·
2022
·
IF 6.14
Jia Chen,Qiaoxian Huang,Qingfu Wang,Yuanfu Ding,Siyu Lu,Lian-Hui Wang,Shengke Li,Ruibing Wang
Inflammation is one of the most common pathological processes involved in numerous diseases. Thus, bioimaging of inflammation is of great significance for the diagnosis and treatment of a variety of inflammatory diseases. Herein, for the first time, we report a supramolecular luminol–AIEgen nanoparticle (SLA NP)-based bioluminescent probe for deep-tissue-inflammation imaging. SLA NPs were facilely fabricated by anchoring adamantane–luminol (AdLum) onto NPs derived from AIEgen–cucurbit[7]uril (AIECB[7]) via CB[7]–Ad host–guest interactions. The designed SLA NPs are capable of efficient bioluminescence resonance energy transfer (BRET) from luminol (donor) to AIEgen (acceptor), enabling deep-tissue penetration and fluorescent imaging. SLA NPs possess uniform particle size and excellent stability and biocompatibility for in vitro and in vivo inflammation imaging, superior to commercial luminol. This work not only demonstrates the enhanced deep-tissue-inflammation imaging of luminol via its BRET pairing with AIEgen NPs but also offers important new insights into the construction of luminol–AIEgen BRET pairs through host–guest complexation.
研究领域:
生物医学工程
·
分子影像学
·
纳米医学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Suyue Liu,Jingjing Tian,Longjiao Zhu,Hongtao Tian,Min Yang,Kunlun Huang,Wentao Xu
To develop a fast, isothermal, enzyme-free and visible method for the on-site detection of silver ions (Ag + ), a ratiometric Ag + sensor was constructed based on a dual-emission ternary nucleotide terbium probe, fabricated by synthesizing the luminescent nanomaterial Luminol-GMP-Tb (LGT). We repositioned the fluorescent properties of the nanomaterial from the perspective of the inherent optical properties of two signal reporters (luminol and Tb 3+ ) through highlighting the absorption, fluorescence steady-state measurement and time-resolved properties. Furthermore, based on the ingenious coordination interaction and energy transfer among Ag + , luminol and GMP-Tb, we hypothesized and confirmed that Ag + induced morphology and luminescence changes of LGT through scanning electron microscopy and lifetime changes as well as fluorescence changes. Meanwhile, by means of a portable ultraviolet detector with dual wavelengths, based on the fluorescence excitation characteristics of each component, different photoluminescence color phenomena presented at different excitation wavelengths. Therefore, visual qualitative detection could be realized under a portable UV lamp with 254 nm for mainly visible green color change while 365 nm as built-in reference for blue-emitting luminol. The ratio calculation method and the enhanced luminescence ratio mechanism made LGT exhibited extremely high selectivity to Ag + . Quantitative detection Ag + was realized in two ranges of 0.1 μM ~ 6 μM and 6 μM ~ 100 μM with a minimum detection limit of 65 nM. The method has been successfully exploited in real water samples with recovery rate between 99.7% and 106.38%, showing broad application prospects in food safety and environmental monitoring for the on-site detection of Ag + .
研究领域:
分析化学
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环境科学
·
食品安全
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DOI
高影响因子
LUMINESCENCE
·
2010
·Vol. 26
·期 6
·397-402
·
IF 1.209
Ying Liu,Zhifeng Fu,Lin Wang
A rapid and simple capillary electrophoresis method coupled with chemiluminescent (CL) detection was proposed for analysis of isoniazid (ISO) based on the enhancement effect of ISO to CL emission of luminol-periodate potassium reaction. Under the optimal conditions, ISO can be assayed in the range of 7.0 × 10 −7 to 3.0 × 10 −5 g mL −1 ( R 2 = 0.9990) with a limit of detection of 3.0 × 10 −7 g mL −1 (signal-to-noise ratio of 3). The whole analysis process can be completed within 2.5 min with a theoretical plate number of 6258. The relative standard deviations of the signal intensity and the migration time were 3.1 and 1.4% for a standard sample at 1.0 × 10 −5 g mL −1 ( n = 5), respectively. The presented novel strategy was successfully applied to the determination of ISO in commercial pharmaceutical preparations and spiked human serum samples. Copyright © 2010 John Wiley & Sons, Ltd.
研究领域:
分析化学
·
药物分析
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Zhiyong Dong,Shiyu Xia,Ala’a Mhmoued Abdllh Alboull,Islam Mohamed Mostafa,Abubakar Abdussalam,Wei Zhang,Shuang Han,Guobao Xu
Transition-metal-based nanozymes show great potential for chemiluminescence (CL) detection due to their stability, excellent catalytic properties, good biocompatibility, and facile synthesis. In this study, bimetallic CoMoO4 nanorods with high peroxidase activity were synthesized using a hydrothermal method, followed by calcination treatment. The as-synthesized CoMoO4 nanorods were utilized as catalysts for CL for the first time. They enhance the CL intensity of the luminol/hydrogen peroxide system by nearly 750-fold. Furthermore, a sensitive and rapid platform for dopamine detection was established based on the quenching effect of dopamine on the CL signal of this system. The proposed CL method for dopamine exhibited a linear range of 10–500 nM and a detection limit of 1.98 nM. Moreover, dopamine in serum samples was successfully measured using a standard addition method, showing a satisfactory recovery of 97.2–104.3%. This work provides robust support for the development of efficient and sensitive platforms for CL detection.
研究领域:
分析化学
·
纳米技术
·
生物化学
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产品关联
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2025
·
IF 2.5
Hui Zhang,Ziqi Wang,Yahui Ji,Junfeng Li,Feifei Chen,Fangxin Du,Gen Liu
The detection of organic pollutants in water was deemed critical for safeguarding aquatic ecosystems, maintaining human health, and upholding water quality standards. In this study, a novel electrochemiluminescence (ECL) sensor was proposed for the sensitive detection of hydroquinone (HQ) in lake water, utilizing a glassy carbon electrode (GCE) modified with silver/luminol-functionalized carbon microspheres (GCE/CM@Ag/Lu). Due to the consumption of H 2 O 2 by HQ, the ECL signal was attenuated, enabling the quantitative detection of HQ. Under optimized conditions, the linear range for HQ ranged from 1.0 × 10 −4 –1.0 × 10 −10 mol L −1 , with a limit of detection (LOD) of 3.3 × 10 −11 mol L −1 (S/N = 3). The proposed ECL sensor shows promising potential to open new avenues for water quality assessment.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2021
·Vol. 13
·期 47
·20205-20212
·
IF 7.79
Haifeng Sha,Bing Yan
The wide applications of metal–organic framework (MOF) luminescent materials in the field of optics have attracted the general attention of researchers. Therefore, the development of simple and multifunctional MOF light-emitting platforms have become a research hotspot. The composites (MnO2@ZIF-8-luminol) were prepared by an in situ synthesis method and room-temperature covalent reaction. The composites and o-phenylenediamine (OPD) constitute a dual emission sensor for detecting alanine aminotransferase (ALT). OPD can be oxidized by MnO2 to 2,3-diaminophenazine (DAP) with yellow fluorescence emission, which inhibits the blue emission of luminol through fluorescence resonance energy transfer (FRET). The presence of tiopronin (TP) will destroy the FRET process, extinguishing the yellow fluorescence emission and restoring the blue fluorescence emission. The special effect between ALT and TP will further reverse the changes in the two fluorescent signals. Moreover, in the detection process, when the blue and yellow fluorescence energies in the system are within a certain range, a new white light emission will be generated, which causes the sensing of ALT to present ternary visualization. In addition, a high-security anti-counterfeiting platform is constructed by using the prepared materials and agarose hydrogels. The anti-counterfeiting platform can encrypt information on demand according to the luminous characteristics of different materials. This study not only provides a typical case of ternary visualization sensing by MOF-based materials but also develops a possible method for the construction of a MOF-based hydrogel anti-counterfeiting platform.
研究领域:
生物化学
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材料科学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 29
·8309-8315
·
IF 4.478
Zhang Huixin,Du Lin,Wei Zhihao,Wang Xuemei,Sojic Neso,Zhou Xin,Wang Zonghua
Electrochemiluminescence (ECL) of luminol is a well-established methodology in analytical chemistry and bioimaging. Developing novel strategies to enhance the ECL signal of this model emitter is a challenging but rewarding task. In this work, we introduced the high-intensity focused ultrasound (HIFU), as a pretreatment means and a non-invasive way to trigger and boost the ECL signal with a 40-fold significant enhancement in the luminol-O 2 system without the addition of exogenous co-reactants. The superoxide anion (O 2 −• ) generated in situ by HIFU was the key initiator for boosting the ECL emission as demonstrated in this study for the first time. This promising co-reactant-free strategy could find potential applications for ultrasensitive ECL detection in the analysis of complex biological entities. Graphical abstract
研究领域:
分析化学
·
生物成像
·
超声技术
·
生物分析
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DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2024
·
IF 3.3
Wan Zhong,Yizhuo Fu,Xueqian Chen,Chao Lu
Electrochemiluminescence (ECL) of the luminol-dissolved O2 system has been applied in many fields, but the role of reactive oxygen species (ROS) in potential-resolved ECL of luminol-dissolved O2 remains ambiguous due to the complexity of the ROS production and the difficulty in detecting ROS. In this work, the function of ROS in potential-dependent luminol-O2 ECL was investigated in detail. Interestingly, the four ECL peaks were observed in the potential-resolved ECL profiles, including a cathodic ECL peak at −0.5 V (ECL-1), two anodic ECL peaks at 0.45 V (ECL-2) and 1.3 V (ECL-3), and a broad and flat peak starting from 1.11 V and ending at 0.35 V (ECL-4, a secondary peak) on the reverse scan backward from upper potentials. The impact of ROS on the different ECL channels was investigated. It was found that superoxide radical (O2•–) could promote all of the above four ECL peaks, while hydroxyl radical (HO•) could only facilitate ECL-1, ECL-3, and ECL-4. Additionally, pathways of all ECL peaks were proposed. The investigation on the role of ROS in potential-resolved ECL of luminol-dissolved O2 will guide the rational regulation and potential applications of luminol ECL in various fields.
研究领域:
分析化学
·
电化学
·
生物分析
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Chuanlin Huang,Wenjuan Zhou,Weijiang Guan,Nengsheng Ye
Background Luminol chemiluminescence (CL) sensing system remains an excellent candidate for application in bioanalysis due to its good water solubility. However, traditional luminol CL system usually requires the addition of oxidizing agents and sensitizers to obtain high efficiency for the improvement of detection sensitivity. Although numerous studies on the nanomaterial-enhanced luminol CL systems have been carried out, there is still great potential to develop inexpensive, readily available and easily handled catalysts to construct simple and effective CL platform for biomolecular sensing. Results Few-layered MoS 2 nanosheets (NS) prepared by sonication-assisted exfoliation of commercially available bulk MoS 2 were found to significantly enhance the CL of luminol‒dissolved oxygen in the absence of additional oxidants. The mechanism study demonstrated that exfoliated MoS 2 NS could catalyze the decomposition of dissolved oxygen by virtue of its exposed active sites on the surface, generating increased reactive oxygen intermediates, which then oxidize luminol and produce intense CL emission. The proposed high-efficiency luminol CL system was then employed for the extremely sensitive identification of dopamine based on the quenching of CL by dopamine. The limit of detection (LOD) for dopamine can be as low as 2.07 nM. Besides, it also works well in the actual urine sample with good recoveries (99.6–100.6 %), confirming the practicability of the method. Significance As a new type of CL catalyst, MoS 2 NS developed in this work are easy to obtain, simple to prepare and can be produced in large quantities, which lays a foundation for extending applicability of MoS 2 NS in the CL field, and provides a new idea for developing simple and highly sensitive CL sensing system.
研究领域:
分析化学
·
生物化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2019
·Vol. 11
·期 36
·16860-16867
·
IF 6.97
Jing-Wen Gao,Miao-Miao Chen,Wei Wen,Xiuhua Zhang,Shengfu Wang,Wei-Hua Huang
Electrochemiluminescence (ECL) nanomaterials are usually deposited compactly on the surface of electrodes, which may cause poor mass transfer of reactants, thereby resulting in low ECL efficiency. In this work, we developed a novel kind of luminescent material denoted as C–Au-luminol nanospheres (C–Au-Lum NSs) by high dispersion of luminophores on porous carbon nanospheres (PCNSs). C–Au-Lum NSs were facilely prepared by the in situ reduction of chloroauric acid with the luminescent reagent luminol (Lum) on the nano-pores of PCNSs. Plenty of luminescent Au-Lum NPs were dispersedly concentrated inside the numerous pores and hollow interiors of PCNSs, effectively increasing the mass transfer of reagents and accelerating the electron transport inside the porous nanospheres. This greatly improved the availability of luminophores and endowed C–Au-Lum NSs with excellent ECL emission. After further integrating with enzymatic circulation and strand displacement, an ultrasensitive ECL biosensor was achieved for the ultrasensitive detection of an important tumor biomarker, mucin1. The logarithmically linear range from 0.1 pg mL−1 to 1 ng mL−1 with the detection limit of 47.6 fg mL−1 (S/N = 3) was achieved, demonstrating the superior performance of C–Au-Lum NSs. This work would provide new ideas for the construction of high-performance ECL sensing platforms for diverse applications.
研究领域:
纳米技术
·
生物传感器
·
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Tong Shao,Xiaolei Song,Yufeng Jiang,Chenchen Wang,Peng Li,Shihao Sun,Dingzhong Wang,Wei Wei
Among various chemiluminescence (CL) systems, luminol-H 2 O 2 system is used extensively due to its cheapness and sensitivity. Herein, 4-hydroxy-3-methoxybenzaldehyde, known as vanillin, was firstly found to be able to catalyze H 2 O 2 very efficiently to produce • OH and O 2 •− , which can be used to enhance the CL of luminol-H 2 O 2 as Co + . In alkaline aqueous solution, vanillin catalyzed the dissociation of H 2 O 2 into active • OH and O 2 •− radicals and accelerated luminol-H 2 O 2 reaction to emit strong CL signal. Combining the stabilizing function of β-CD, CL intensity of luminol-H 2 O 2 was enhanced further. Thus, dual-signal amplification of luminol-H 2 O 2 chemiluminescence based on the catalyzing function of vanillin and the stabilizing function of β-CD was proposed and its mechanism was explored deeply in the manuscript. Interestingly, vanillin is a highly prized flavor compound broadly used as food additive, however, the excessive intake of vanillin is harmful to human and thus the determination of vanillin is very important. On the basis of the luminol-β-CD-H 2 O 2 /vanillin reaction, a low-cost, rapid and simple CL sensor has been established to detect vanillin. The sensor was able to detect vanillin in the range of 1.0 μM ∼ 75 μM with a detection limit of 0.89 μM (S/N = 3). It can also be used for CL imaging detection with satisfactory results.
研究领域:
分析化学
·
食品科学
·
化学传感器
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 5
·1924-1928
·
IF 1.821
Guang-Wen Li,Lei Hong,Meng-Sha Tong,Hao-Hua Deng,Xing-Hua Xia,Wei Chen
In this study, it was found that tannic acid could inhibit the chemiluminescence (CL) intensity of luminol–H2O2–cupric oxide nanoparticles significantly. Based on this inhibition effect, a novel and highly sensitive flow injection method with inhibited chemiluminescence was developed for the determination of tannic acid. Under optimum conditions, the net CL intensity was proportional to the concentration of tannic acid over the range of 10–100 nM and the detection limit was 2.6 nM. The relative standard deviation (RSD) for six repeated determinations of 80 nM tannic acid was 2.8%. Note that this method has been successfully used for the analysis of tannic acid in real Chinese gall samples.
研究领域:
分析化学
·
纳米技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）