Micromachines
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2023
·Vol. 14
·期 3
·660
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IF 3.4
Youqiang Xing,Yun Wang,Lei Liu,Ze Wu
As a major public health issue, early cancer detection is of great significance. A field-effect transistor (FET) based on an MoS2/C60composite nanolayer as the channel material enhances device performance by adding a light source, allowing the ultrasensitive detection of cancer-related miRNA. In this work, atomic layer deposition (ALD) was used to deposit MoS2layer by layer, and C60was deposited by an evaporation coater to obtain a composite nanolayer with good surface morphology as the channel material of the FET. Based on the good absorption of C60by blue-violet light, a 405 nm laser was selected to irradiate the channel material, improving the function of FET biosensors. A linear detection window from 10 pM to 1 fM with an ultralow detection limit of 5.16 aM for miRNA-155 was achieved.Keywords:ultrasensitive detection;FET;MoS2/C60composite nanolayer
研究领域:
生物医学工程
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材料科学
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PubMed
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 54
·39908-39920
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IF 3.9
Dabin Liu,Wenzhang Zhu,Bin Qiu,Shiqian Zhang
Research has shown that the expression level of microRNA-155 (miRNA-155) is positively correlated with clinical stage and depth of invasion in patients with cervical cancer and cervical intraepithelial neoplasia and tends to be highly expressed. Therefore, it is very important to develop sensitive miRNA-155 analysis methods for the early diagnosis, treatment, and prognostic evaluation of cervical cancer. In this study, a near-infrared light-driven fluorescent biosensor based on the metal-enhanced fluorescence effect of polydopamine-coated upconversion nanoparticle (UP/Au) and two toehold-mediated strand displacement (TMSD) steps was constructed for the detection of miRNA-155. The target miRNA-155 can undergo a TMSD1 reaction with single-stranded DNA1 (ssDNA1) modified on wrinkled silica (WSNs) to form the ssDNA1/miRNA-155 complex and expose toehold 2. Through the TMSD2 reaction, ssDNA2 adsorbed on the UP/Au surface reacts with ssDNA1 to form the ssDNA1/ssDNA2 complex, which replaces miRNA-155 and enables the recovery of the UP/Au fluorescence signal. The target miRNA-155 was reacted with the new ssDNA1 to amplify the detection signal, with a detection range of 0.5–20 pM and a detection limit of 19.76 fM for miRNA-155. In addition, the fluorescent biosensor has been applied for the analysis of miRNA-155 in serum samples, indicating its good practicality.
研究领域:
生物传感技术
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分子诊断
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癌症研究
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DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2026
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IF 4.6
Yaoxuan Wang,Haiyan Wang,Xianchuan Dong,Jing Sui,Feifei Zhang,Zonghua Wang
Here presents a sensing strategy for the detection of miRNA-155 based on a multi-amplification strategy, which involves nucleic acid recognition-induced multiple DNA release and Exonuclease III (Exo III)-assisted target recycling. Initially, cDNA-magnetic bead bioconjugates were employed to capture the target miRNA, triggering the release of two methylene blue (MB)-modified messenger DNAs (mDNAs). This step effectively doubles the amount of mDNAs-MB released, contributing to initial signal amplification. Subsequently, the target miRNA is recycled via Exo III-mediated cleavage, leading to the release of additional mDNAs-MB and further enhancing the signal. Finally, each capture probe (P1) is designed to hybridize with two mDNAs-MB, enabling dual signal recognition. As a result, the square wave voltammetry (SWV) measurements detected a significantly amplified MB peak current. The overall detection strategy integrates three synergistic amplification approaches: the release of multiple mDNAs, Exo III-assisted target miRNA recycling, and dual hybridization of mDNAs to P1. The miRNA-155, a recognized biomarker for breast cancer diagnosis, was used as the target to evaluate the biosensor's performance. The proposed electrochemical biosensor demonstrated a broad detection range from 0.1 pM to 1 mM and achieved a low detection limit of 0.026 pM, confirming its high sensitivity and potential applicability in cancer diagnostics.
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高影响因子
Science China-Chemistry
·
2020
·Vol. 63
·期 5
·731-740
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IF 6.356
Wang Yingfei,Li Yuyi,Zhang Yue,Ren Kewei,Ju Huangxian,Liu Ying
Array based detection techniques with fluorescence signal reading is a powerful tool for multiple targets analysis. However, when applied fluorescence array for microRNA detection, time-consuming multi-steps surface signal amplification is usually required due to the low abundance of microRNA in total RNA expressions, which impairs detection efficiency and limits its application in point of care test (POCT) manner. Herein, DNA cascade reactors (DCRs) functionalized photonic crystal (PC) array was fabricated for express and sensitive detections of miRNA-21 and miRNA-155. DCRs were assembled by interval conjugation of self-quenched hairpin DNA probes to single strand DNA nanowire synthesized by rolling circle amplification, which generated cascade DNA hybridization reactions in response to target miRNA with instant fluorescence recovery signal. PC array patterns with multi-structure colors further amplified fluorescence with their respective photonic bandgaps (PBGs) matching with the emission peaks of fluorescence molecules labelled on DCRs. The as-prepared DCRs functionalized PC array demonstrated express and sensitive simultaneous detections of miRNA-21 and miRNA-155 with hundreds fM detection limits only in 15 min, and was successfully applied in fast quantifications of low abundance miRNAs from cell lysates and spiked miRNAs from human serum, which would hold great potential for disease diagnosis and therapeutic effect monitoring with a POCT manner.
研究领域:
分子生物学
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纳米技术
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生物医学工程
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临床诊断
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高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Xiaofang Liu,Qun Wang,Jiawei Li,Zhan Diao,Jingzhou Hou,Danqun Huo,Changjun Hou
Simultaneous detection of multiple breast cancer-associated miRNAs significantly raises the accuracy and reliability of early diagnosis. In this work, disposable carbon fiber paper serves as the biosensing interface, linking DNA probes via click chemistry to efficiently capture targets and signals efficiently. DNA probes have multiple recognition domains that trigger a cascade reaction through the helper probes and targets, resulting in two signals output. The signals are centrally encapsulated in the pore of the MIL-88(Fe)-NH2. The signal carriers are directed by signal probes to the recognition domains that correspond to the DNA probes. The biosensor is selective and stable, and it can quantify miRNA-21 and miRNA-155 simultaneously with detection limits of 0.64 and 0.54 fmol/L, respectively. Furthermore, it demonstrates satisfactory performance in tests conducted with normal human serum and cell lysate. Overall, this method makes a satisfactory exploration to realize an inexpensive and sensitive biosensor for multiple biomarkers.
研究领域:
生物技术
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生物传感器
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癌症研究
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分子生物学
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分析化学
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jiaxin Peng,Xiaomin Hang,Huiyi Wang,Kairen Zhao,Honghong Wang,Haoming Yu,Li Wang
MicroRNAs (miRNAs) have emerged as powerful biomarkers for various diseases diagnosis, yet their clinical application is greatly plagued by the lack of reliable and rapid detection methods. Here, a simple, fast, and accurate point-of-care testing (POCT) platform for miRNA-155 detection was developed by interfacing self-primer exponential amplification reaction (SPEXPAR) with colorimetric and POCT lateral flow assay (LFA). In this platform, a hairpin template was designed for exponential amplification reaction, which could effectively avoid nonspecific template interactions and eventually effectively avoid undesirable background amplification; the colorimetric assay is based on the SPEXPAR amplicons induced gold nanoparticles (AuNPs) color change; and the LFA detection principle involves the amplicons hybridization with designed probes. Under the optimized conditions, the colorimetric assay could detect miRNA-155 as low as 0.41 pM in less than 35 min; and LFA could detect as low as 100 pM in less than 40 min. Furthermore, the method was adopted to detect miRNA-155 in human serum samples with satisfactory recoveries. In summary, this study offered a promising analytical tool for molecular diagnostic testing application.
研究领域:
生物技术
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医学诊断
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分子生物学
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高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Weipan Peng,Shenghui Li,Yating zhang,Qiuting Zhang,Wenna Li,Houyu Han,Dianming Zhou,Sheng Wang,Jin Chang,Xiaoqun Gong
Tumors progression is usually characterized by proliferation, migration, and angiogenesis , and it leads to uncontrolled tissue growth and blocks the mechanism of apoptosis. Therefore, targeted angiogenesis and anti-apoptosis is considered promising therapeutic strategy for cancer prevention and treatment. Herein, we report a versatile and intelligent MnO 2 -PEI-DNAzymes (MnPDs) nanodevice for self-sufficient anti-tumor angiogenesis and pro-apoptosis synergistic gene silencing, coupling multi-target bimodal in situ imaging. Intracellularly, the multifunctional charge-reversal MnPDs undergo reduction and lysis by the overexpressed GSH, actualizing enhanced MR imaging and sufficient DNAzyme cofactors in tumor tissue, and enabling miRNA-155 and telomerase biomarkers dual-fluorescence imaging. Under the precise multimodal imaging, the dual DNAzymes enable self-sufficient Early growth response-1 (EGR1) and Survivin (SUR) mRNAs synergistic silencing, effectively inhibiting tumor angiogenesis and anti-apoptotic. Considering that accurate and efficient therapy has become a trend in cancer therapy, our proposed self-sustaining multifunctional nanodevice is promising for broad clinical applications.
研究领域:
癌症生物学
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纳米技术
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基因治疗
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分子成像
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生物医学工程
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
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IF 5.1
Tingyi Huang,Li Tan,Wenyi Zhang,Ying Ma,Ziqi Lian,Benlin Hong,Jianqiang Hu
Highly sensitive and simple biomarker determination is crucial for diagnosis and treatment of early diseases. However, it remains a tremendous challenge for rapid, accurate and real time detection of biomarkers at the early stage of diseases due to its extremely low content and serious interference. Herein, we have successfully constructed an ultrasensitive electrochemiluminescence (ECL) biosensor based on 2,6-bis(2-benzimidazolyl)pyridine platinum(II) (BZIMPY-Pt) complexes with aggregation-induced electrochemiluminescence (AIECL) phenomenon, which was utilized to detect miRNA-155 at early diseases. The BZIMPY-Pt complexes with AIECL properties and had high affinity with G-quadruplex DNA compared to other square planar pyridyl Pt(II) complexes, which exhibited 13-fold enhancements in ECL intensity in the aggregated state. The constructed biosensor possessed high sensitivity, wide linear range (1 fM-10 nM), low limit of detection (∼0.22 fM), high selectivity and good stability and reproducibility for miRNA-155 detection. The ECL biosensor was successfully applied to determine miRNA-155 in the serum samples of early-stage diagnose. This AIECL-based strategy overcomes traditional limitations in low-abundance biomarker analysis through synergistic signal amplification and background suppression mechanisms, which is potential for clinical diagnosis of early diseases.
研究领域:
生物传感器
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生物分析化学
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纳米技术
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分子诊断学
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电化学
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临床化学
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金属配合物药物
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生物医学工程
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DOI
高影响因子
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