TALANTA
·
2020
·
IF 5.339
Wenyue Zhang,Wenhui Hao,Xuetao Liu,Xiru Sun,Jingli Yan,Yucong Wang
A visualized assay for miRNAs detection has been developed in this work. The presented method is based on a combination of enzyme-free amplification cascades of catalyzed hairpin assembly (CHA) and hybridization chain reaction (HCR) and fluorescence quenching of dual-emission ratiometric fluorescent probes (RF probes). MiRNAs can efficiently initiate enzyme-free amplification reactions (CHA and HCR) and produce the long nicked dsDNAs with a lot of glucose oxidases (GOD) on the surface of dynabeads bridged by the GOD-labeled hairpin DNA probes . Hydrogen peroxide (H 2 O 2 ) is generated by oxidation of glucose catalyzed by GOD, which can quench the outer green fluorescence without affecting the internal red fluorescence of RF probes. Therefore, increased miRNA amount can result in change of the two fluorescence intensity ratios of RF probes with continuous color changes from green to red under a UV lamp, which can be easily recognized by naked eye. The proposed assay exhibits high sensitivity toward let-7a with dynamic range from 10 −13 M to 10 −8 M, and which is applied successfully to detecting let-7a in the small RNA samples.
研究领域:
分子生物学
·
生物化学
·
医学诊断
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yadan Zhang,Xiaoyu Zhang,Tianhan Kai,Lin Zhang,Anping Li
Plant-derived exosome-like nanovesicles (ELNs) are nano-sized vesicles extracted from edible plants. Lycium ruthenicum Murray (LRM) has been gaining increasing attention due to its nutritional and medicinal value, but the ELNs in LRM has not been reported. In this study, LRM-ELNs were obtained, and the proteins, lipids, microRNAs (miRNAs) and active components in LRM tissues and LRM-ELNs was analyzed by LC-MS/MS, LC-MS, high-throughput sequencing techniques, and physical and chemical analysis. LRM-ELNs can be uptaken by PC12 cells through macropinocytosis and caveolin-mediated endocytosis primarily. Transcriptomic and western blot experiments indicate that LRM-ELNs can inhibit Aβ-induced apoptosis in PC12 cells through the MAPK and PI3K/AKT signaling pathways, with miRNAs playing a crucial role. These results indicate that LRM-ELNs have the protection effect on PC12 cells and can be considered as dietary supplements for alleviating neurodegenerative diseases.
研究领域:
神经科学
·
药理学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2022
·Vol. 37
·期 8
·1902-1913
·
IF 4.109
Ruike Wang,Zuntao Wu,Rundong Liu,Lin Bai,Yan Lin,Yue Ba,Hui Huang
Numbers of emerging evidence suggest that lead (Pb) exposure contributes to cognitive decline and might also increase the risk of Alzheimer's disease (AD) dementia in the elderly by increasing the beta-amyloid burden. Here, we aimed to characterize the effects of Pb on the post-transcriptional regulators, microRNAs (miRNAs), which may participate in AD pathogenesis. At first, early chronic Pb exposure on neuronal miRNAs expression with increasing aging was profiled to elucidate the association of three selected miRNAs with β-site APP-cleaving enzyme 1(BACE1), a rate-limiting enzyme for β-amyloid (Aβ) production. Next, we verified changes in BACE1 were observed by regulating miRNAs expression in vitro. While Pb promoted BACE1 levels, BACE1 levels were reduced in SH-SY5Y cells with miR-124-3p mimic, suggesting for the first time that miR-124-3p/BACE1 pathway modulation is critically involved in Pb-induced AD-like amyloidogenic processing. Findings from this study could provide new insight into the molecular mechanisms of Pb-associated neurodegenerative pathogenesis from an epigenetic perspective.
研究领域:
神经科学
·
分子生物学
·
毒理学
·
表观遗传学
✓ 已验证
DOI
PubMed
高影响因子
Carbohydrate Polymer Technologies and Applications
·
2025
·
IF 6.5
Hurong Ge,Peipei Ma,Qing Huang,Shijie Wei,Zhizhong Wang
Objectives: To study the encapsulation of resveratrol by three cyclodextrin derivatives, investigate the impact of encapsulation technology on its anti-fatigue effect, and explore the anti-fatigue mechanism of the encapsulated resveratrol via transcriptomics analysis of exosomal miRNAs. Methods (1) UV spectroscopy and nuclear magnetic resonance (NMR) were used to investigate the encapsulation and encapsulation modes of resveratrol by β -CD, HP- β -CD and CM- β -CD; and the phase solubility method was used to calculate the solubilisation multiplicity of resveratrol by the three cyclodextrin derivatives. (2) Male C57BL/6 J mice were randomly divided into: fatigue model group (FM), FM+resveratrol group (SF+Res), FM+ β -CD, FM+HP- β -CD, FM+CM- β -CD, FM+ β -CD+Res, FM+HP- β -CD+Res, FM+CM- β -CD +Res, and after 30 days of rearing, the mice were subjected to exhaustion swimming experiment to detect fatigue-related indexes and observe the effects of the three cyclodextrin derivatives on the anti-fatigue effects of resveratrol. (3) Male C57BL/6 J mice were randomly divided into Control, FM, and FM+HP- β -CD+Res. After 30 days of rearing, except for the Control group, the remaining mice were subjected to exhaustion swimming experiments, and exosomes were extracted from cardiac tissue, skeletal muscle tissue, and liver tissue of the three groups of mice, and the transcriptomics technology was applied to sequence the exosomal miRNAs and to explore the mechanism of the effect of the HP- β -CD+Res envelope on fatigue. Results (1) The inclusion ratios of β -CD, HP- β -CD, and CM- β -CD encapsulated resveratrol were 1:1, 1:2, and 1:1, and the solubilisation multiplicities of resveratrol were 23, 39, and 21 fold, respectively; β -CD and CM- β -CD encapsulated Res, which was the benzene ring with two hydroxyl‑terminal ends of Res entered the cavity from the small-ported end of the cyclodextrins; HP- β -CD encapsulated Res, which was the Res containing one hydroxyl‑terminated benzene ring enters the cavity from the wide-mouth end of HP-β-CD. (2) Compared with the FM+Res group, all three inclusions were able to prolong the exhaustion swimming time of mice, with HP- β -CD+Res being the most effective. (3) After HP- β -CD+Res acted on the fatigued mouse model, cardiac tissue showed 1726 differentially expressed exosomal miRNAs, of which 840 were up-regulated and 886 were down-regulated, reversing the fatigue-induced changes in 64.96 % of the exosomal miRNAs (1170 shared differential exosomal miRNAs); Skeletal muscle tissue showed 1948 differentially expressed exosomal miRNAs, of which 718 were up-regulated and 1230 were down-regulated, reversing 80.57 % of the fatigue-induced changes in exosomal miRNAs (1576 shared differential exosomal miRNAs); Liver tissue showed 1442 differentially expressed exosomal miRNAs, of which 451 were up-regulated and 991 were down-regulated, reversing 84.31 % of fatigue-induced changes in exosomal miRNAs (1214 shared differential exosomal miRNAs). Target gene enrichment analysis of differentially expressed exosomal miRNAs in cardiac tissue, skeletal muscle tissue, and liver tissue had similar results, and the biological processes involved in HP- β -CD+Res were related to biological_process, signal transduction, G protein-coupled receptor signaling pathway, etc., and cellular components such as membrane, cytoplasm, integral component of membrane, etc., and molecular functions are closely related to protein binding, metal ion binding, molecular_function, etc.; mainly enriched in Axon guidance, MicroRNAs in cancer, Apoptosis and other signalling pathways. Conclusions (1) All three cyclodextrin derivatives encapsulated increased the solubility of resveratrol, with HP- β -CD having the best effect. (2) All three cyclodextrin derivative encapsulation techniques improved the anti-fatigue effect of resveratrol, with HP- β -CD being the most effective. (3) HP- β -CD+Res was able to induce differential expression of exosomal miRNAs in cardiac tissue, skeletal muscle tissue and liver tissue of fatigued mice.
研究领域:
药理学
·
分子生物学
·
生物信息学
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2026
·
IF 17.3
Jia Chen,Jianping He,Mengmeng Xiao,Mengyan Wang,Yu Xia,Fang Deng,Kui Zhou,Donghan He,Mingyin Luo,Yuehua Ke,Zhiyong Zhang
Carbon nanotube field-effect transistor (CNT-FET) biosensor, featured with high sensitivity, label-free operation and miniaturization, has emerged as a promising candidate platform to detect trace microRNAs (miRNAs), which are critical disease biomarkers whose aberrant expression is closely associated with cancer progression. However, the complex assembly process of probes on gates often damages the performance of CNT FETs, which makes sensitivity of the sensor unable to meet reliable detection of ultralow concentration biomarkers. Here, we report a detachable CNT FET biosensor with a modular gate chip for ultrasensitive and reproducible miRNA detection. Specifically, the sensitive gate array and the CNT FET are separately fabricated as two modules with reserved liquid metal connection ports. After biofunctionalized process, the sensitive gate modules are assembled to FET modules to form the complete biosensors. This design not only avoids damage to CNT FETs caused by harsh environments during the assembly process of biological probes, but also allows the CNT FETs to be reused repeatedly. Using miRNA-21 as a model target, the modular biosensors exhibit ultrahigh sensitivity (limit of detection of 0.36 aM) and exceptional reproducibility (a response variation below 5.1%). Clinical serum analysis of 48 samples reveals significantly elevated miRNA-21 levels in liver, lung, and breast cancer patients compared with healthy individuals, with results showing excellent agreement with qRT-PCR (R2 = 0.98). The CNT FET biosensor with a modular sensitive gate provides a robust paradigm for cost-effective, decentralized miRNA screening.
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xingjie Wu,Hong Yang,Wei Li,Ling Tao,Qingteng Wang,Yingzu Jin,Xuecheng Sun,Xiangchun Shen
The prompt diagnosis of acute myocardial infarction (AMI) for patients with acute ischemic-type chest pain is critical for timely AMI treatment and lowering its casualty rate. In this article, we developed a multiparametric detection technique for AMI related microRNAs (miRNAs), which is based on the polymerization induced fluorescence-off (PIFO) signal amplification technique. PIFO can be implemented on a specially designed microfluidic chip equipping a fluorescence sensor, which is able to profile 3 miRNAs simultaneously in 45 min with a detection limit of 6100 miRNAs copies. Comparing with other polymerization-based signal amplification techniques, PIFO is rapid, oxygen-independent, multiparametric, and can be performed in ambient environment. The miniaturized PIFO device can be further applied to analyze AMI related miRNAs in clinical serum samples, and displays excellent performance in distinguishing AMI patients from patients with other cardiovascular diseases. Moreover, PIFO shows a high accuracy for AMI diagnosis among patients with low hypersensitivity troponin I level (< 0.1 ng mL −1 ) as a result of the early and fast respond to AMI onset by miRNAs.
研究领域:
医学诊断
·
分子生物学
·
生物传感器
✓ 已验证
DOI
高影响因子
ACS Nano
·
2024
·
IF 15.8
Wei Wang,Lei Liu,Jianxiong Zhu,Youqiang Xing,Songlong Jiao,Ze Wu
In biomedical testing, artificial intelligence (AI)-enhanced analysis has gradually been applied to the diagnosis of certain diseases. This research employs AI algorithms to refine the precision of integrative detection, encompassing both visual results and fluorescence spectra from lateral flow assays (LFAs), which signal the presence of cancer-linked miRNAs. Specifically, the color shift of gold nanoparticles (GNPs) is paired with the red fluorescence from nitrogen vacancy color centers (NV-centers) in fluorescent nanodiamonds (FNDs) and is integrated into LFA strips. While GNPs amplify the fluorescence of FNDs, in turn, FNDs enhance the color intensity of GNPs. This reciprocal intensification of fluorescence and color can be synergistically augmented with AI algorithms, thereby improving the detection sensitivity for early diagnosis. Supported by the detection platform based on this strategy, the fastest detection results with a limit of detection (LOD) at the fM level and the R2 value of ∼0.9916 for miRNA can be obtained within 5 min. Meanwhile, by labeling the capture probes for miRNA-21 and miRNA-96 (both of which are early indicators of breast cancer) on separate T-lines, simultaneous detection of them can be achieved. The miRNA detection methods employed in this study may potentially be applied in the future for the early detection of breast cancer.
研究领域:
生物医学工程
·
癌症诊断
·
纳米技术
·
人工智能
✓ 已验证
DOI
PubMed
高影响因子
PeerJ
·
2021
·
IF 2.984
Wenwen Wang,Wenwen Zhang,Yuanjing Hu
Background Chemotherapy resistance, especially platinum resistance, is the main cause of poor prognosis of ovarian cancer. It is of great urgency to find molecular markers and mechanism related to platinum resistance in ovarian cancer. Methods One mRNA dataset ( GSE28739 ) and one miRNA dataset ( GSE25202 ) were acquired from Gene Expression Omnibus (GEO) database. The GEO2R tool was used to screen out differentially expressed genes (DEGs) and differentially expressed miRNAs (DE-miRNAs) between platinum-resistant and platinum-sensitive ovarian cancer patients. Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis for DEGs were performed using the DAVID to present the most visibly enriched pathways. Protein–protein interaction (PPI) of these DEGs was constructed based on the information of the STRING database. Hub genes related to platinum resistance were visualized by Cytoscape software. Then, we chose seven interested hub genes to further validate using qRT-PCR in A2780 ovarian cancer cell lines. And, at last, the TF-miRNA-target genes regulatory network was predicted and constructed using miRNet software. Results A total of 63 upregulated DEGs, 124 downregulated DEGs, four upregulated miRNAs and six downregulated miRNAs were identified. From the PPI network, the top 10 hub genes were identified, which were associated with platinum resistance. Our further qRT-PCR showed that seven hub genes (BUB1, KIF2C, NUP43, NDC80, NUF2, CCNB2 and CENPN) were differentially expressed in platinum-resistant ovarian cancer cells. Furthermore, the upstream transcription factors (TF) for upregulated DE-miRNAs were SMAD4, NFKB1, SMAD3, TP53 and HNF4A. Three overlapping downstream target genes (KIF2C, STAT3 and BUB1) were identified by miRNet, which was regulated by hsa-miR-494. Conclusions The TF-miRNA–mRNA regulatory pairs, that is TF (SMAD4, NFKB1 and SMAD3)-miR-494-target genes (KIF2C, STAT3 and BUB1), were established. In conclusion, the present study is of great significance to find the key genes of platinum resistance in ovarian cancer. Further study is needed to identify the mechanism of these genes in ovarian cancer.
研究领域:
肿瘤学
·
分子生物学
·
基因组学
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 17
·5245-5253
·
IF 4.019
Yihan Zhao,Yu Wang,Su Liu,Chonglin Wang,Jiaxu Liang,Shasha Li,Xiaonan Qu,Rufeng Zhang,Jinghua Yu,Jiadong Huang
We have formulated a rapid and high-efficiency fluorescent biosensing platform based on a target-activated triple-helix molecular switch (THMS)-conformation-change-induced exponential rolling circular amplification (RCA) strategy for the ultrasensitive detection of miR-21. In this strategy, there are several aspects that are worthwhile. First, the functionalized THMS, comprising a typical triplex structure (T-A·T), specific recognition sequence for nicking endonuclease, complementary sequence for miR-21, and RCA product-annealing sequence, was concurrently used to perform signal transduction with one fluorophore and one quencher. As compared to the traditional double-helix molecular switches or molecular beacons, one of the biggest differentiating factors is that the properties of THMSs are independent of any specific binding sequence that they may contain. As far as we know, this is the first time that an ingeniously designed THMS not only contains the primer for exponential RCA, but also functions as the tracer for fluorescence assay. In the presence of miR-21, targets can induce conformation changes in THMS with the release of the trapped DNA segment (P), which, in turn, can activate the first run of the RCA process. Meanwhile, the RCA reaction is also initiated by the formation of a similar primer (SP) as the trapped DNA through a continuous “extension-nicking” reaction. Secondly, the resultant first-generation RCA product consists of numerous tandem repeated regions that can attach to countless THMSs, resulting in the release of the trapped DNA segment (P) for initiating the second run of the RCA reaction. Significantly, a large amount of THMSs were continuously consumed to yield a remarkably strong fluorescent signal. In addition, this biosensor was demonstrated to exhibit improved sensitivity owing to the high efficiency and rapid amplification kinetics of the exponential RCA and high selectivity toward miR-21 with a limit of detection as low as 1.1 aM. Hence, the target-mediated THMS-conformation-change-initiated exponential RCA strategy presents an optimal detection performance toward analytes for potential applications in related fundamental research and clinical diagnosis.
研究领域:
分子生物学
·
生物化学
·
医学诊断
✓ 已验证
DOI
PubMed
高影响因子
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2018
·Vol. 35
·期 2
·1700330
·
IF 4.384
Yuting Jiao,Mengting Zhu,Xiaomeng Wang,Zhen Zhang,Shusheng Zhang
A novel finding is herein reported that the bidirectional-signal nanoclusters self-assemble simultaneously on the nanoflowers as a result of four-way folding (FWF) nanoprobes and DNA rolling circle replication reactions. The functionalized FWF nanoprobe containing the identification region for two targets monitoring and the trigger region for amplification signals is first used to activate the clustered amplification for two cancer-related microRNAs (miRNAs) assays in single cells. Furthermore, the self-assembled nanoclusters with two-way amplification signals can provide more reliable information in situ in individual cell. Importantly, this new method can significantly distinguish cancer cells from normal cells and identify changes in the expression levels of cancer-related miRNAs for single cells. These findings have exciting potential to provide new opportunities for detection and enhance the accuracy of early disease diagnosis.
研究领域:
纳米技术
·
分子生物学
·
癌症研究
·
生物医学工程
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xinqin He,Kaixuan Chen,Mengjie Yang,Xia Zhang,Hongrui He,Bin Qiu,Hong Jiang
Endometriosis is a chronic inflammatory disease that often occurs in women of childbearing age. Studies indicate that the abnormal expression of various miRNAs, such as miRNA-21, miRNA-122, miRNA-199a and miRNA-191, in patients with endometriosis, making them diagnostic biomarkers for early-stage detection. Herein, using miRNA-21 as the target model, a “turn-on” biosensor was developed, employing upconversion nanoparticle (UCNP) as stable fluorescent donor in complex biological samples, along with graphene oxide (GO) and BHQ2 as dual receptors that provide strong adsorption and efficient fluorescence quenching. The UCNP-H1 nanoprobes were fabricated by conjugating a specific miRNA-recognizing single-stranded DNA aptamer (H1) onto the UCNP. Upon binding to miRNA-21, UCNP-H1 nanoprobes are released from GO, leading to significant fluorescence restoration. The fluorescence intensity increased proportionally with the miRNA-21 concentration, establishing a highly accurate “turn-on” biosensing platform. Under optimal conditions, the biosensor demonstrated a linear response in the concentration range of 0.5–20 nM, with a detection limit as low as 0.83 nM. The recoveries of the spiked serum samples ranged from 98.18 % to 108.82 %, suggesting the applicability of the proposed biosensor for miRNA-21 detection in biological samples. Additionally, this biosensor can also be used to quantify other miRNAs related to endometriosis by altering complementary sequences to achieve accurate early diagnosis.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Xiangyu Ma,Kun Qian,Onome Ejeromedoghene,Martha Kandawa-Schulz,Wei Song,Yihong Wang
In this work, we report AuNPs-decorated pyrolyzed Co-BDC nanosheets (p-Co-BDC/AuNPs) as high-performance electrocatalyst for developing an electrochemical platform. p-Co-BDC/AuNPs as a new electrocatalyst showed superior electrocatalytic activity towards the electrochemical oxidation of methylene blue (MB). Besides, magnetic p-Co-BDC/AuNPs can be well immobilized on the magnetic glassy carbon electrode without further assistance. The oxidation of MB can be reduced by ascorbic acid . Inspired by this phenomenon, an electrochemical biosensor was constructed based on multiple signal amplification for the diagnosis of miRNAs . Firstly, p-Co-BDC/AuNPs enhanced the electrochemical oxidation of MB. Then, strand displacement amplification reaction can form lots of double helix structure DNA to embed more MB molecules. Finally, ascorbic acid in the electrolyte was utilized to reduce the oxidation of MB and improve the electrochemical signal of MB electro-oxidation. The linear detection range for the detection of miRNAs is 100 aM to 10 nM, and the limit of detection is 86 aM. Furthermore, the constructed biosensor also displayed satisfactory selectivity , good reproducibility, and excellent recovery in the detection of real samples. We are convinced that our proposed multiple signal amplification strategy will provide more promising methods for the diagnosis of cancer.
研究领域:
生物化学
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）