SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Liping Lu,Chang Liu,Tianfang Kang,Xiayan Wang,Guangsheng Guo,Wujian Miao
An ultrasensitive and selective miRNA biosensor based on the luminol-H 2 O 2 electrochemiluminescence (ECL) system using hemin as a catalyzer for in situ ECL signal enhancement was reported. DNA probe was tethered onto the electrodeposited Au nanoparticles on a glassy carbon electrode, and the remaining active sites were then blocked with glucose oxidase (GOD). Subsequently, followed by miRNA hybridizing with the DNA probe, hemin molecules acted as the essential enhancer of ECL were intercalated into the DNA/miRNA duplex structure. The fully constructed biosensor was placed in a phosphate buffered solution containing luminol and glucose, where glucose was enzymatically decomposed by GOD to H 2 O 2 . Formation of reactive oxygen species (O 2 − ) from H 2 O 2 , which was a key step for luminol ECL production, was efficiently catalyzed by the intercalated hemin. Thus, enhanced ECL signal was obtained. Under the optimal conditions, this miRNA biosensor could sensitively and selectively detect target miRNA over approximately seven orders of magnitude between 0.050 and 1.0 × 10 5 pM, with a limit of detection of 50 fM. miRNAs extracted from the A459 cell line were quantified with the biosensor. The ECL enhancement strategy presented in this paper could open a new route for the development of extremely sensitive ECL based biosensors.
研究领域:
生物化学
·
分析化学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Chemistry-An Asian Journal
·
2025
·
IF 3.3
Xiaoqian Zhou,Haicheng Song,Xinying Zhang,Lifei Chen,Yingying Chen,Zhenzhen Li,Lingyan Feng
MXene, a two-dimensional nanomaterial, has metal conductivity, high electronegativity, functionalized with surface groups, which make it widely applicable in catalysis and biosensing. However, studies on the principle of enhanced electrochemiluminescence (ECL) by MXene composites and the improvement of their performance in catalyzing the ECL reaction are still in their infancy. In this study, gold nanoparticles (AuNPs) are obtained by mild reductive reduction and loaded in situ on the Ti 3 C 2 T x MXene surface to form the composites (AuNPs@MXene). In oxygenated PBS test buffer, AuNPs@MXene enhance the ECL emission of silver nanoclusters (AgNCs) with aggregation-induced electrochemiluminescence (AIECL) properties as luminophore. Approximately 7.5-fold enhancement of ECL signals is obtained by using two ECL enhancement strategies: an efficient AIECL emitter and a co-reaction accelerator. The special nucleic acid structure with “Three Way Junction (TWJ)” enables an ultra-sensitive detection of microRNA, providing an efficient and ultra-sensitive method for microRNA detection. The biosensor achieves a wide detection range of microRNA-21 from 100 aM to 1 nM, with a low detection limit of 31 aM, and exhibits excellent stability, selectivity and high reproducibility in real samples.
研究领域:
生物传感
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELLS TISSUES ORGANS
·
2022
·
IF 2.208
Zhou Yafu,Yan Jianhua,Chen Huiguo,Zhou Wenwu,Yang Jinsong
GINS subunit complex 4 (GINS4) is fundamental to DNA replication and G1/S phase transition of the cell cycle in eukaryotes. Further, recent studies implied that GINS4 can mediate the progression of several tumors, but its mechanism in lung adenocarcinoma (LUAD) is not clarified. Therefore, the role of GINS4 in LUAD was explored. miR-133a-3p and GINS4 mRNA expression were tested through qRT-PCR. Protein levels of the two genes were assayed by Western blot. Their targeting relationship was predicted and verified by bioinformatics prediction and dual-luciferase analysis. The functions of miR-133a-3p and GINS4 in LUAD were evaluated by Transwell, wound healing, CCK-8, and flow cytometry assays. MTT assay and caspase-3 activity detection were utilized to measure the regulation of miR-133a-3p/GINS4 in the cisplatin sensitivity of LUAD cells. The results showed that GINS4 was highly expressed in LUAD cells (p < 0.05). miR-133a-3p, the upstream gene of GINS4 in LUAD, negatively mediated GINS4 expression. Moreover, overexpressing GINS4 enhanced the proliferative, migratory, and invasive abilities of LUAD cells and inhibited cell apoptosis and the sensitivity to cisplatin, while overexpressing miR-133a-3p caused the contrary results. However, the promoting effects of GINS4 overexpression on LUAD could be offset by miR-133a-3p overexpression. miR-133a-3p could regulate malignant behaviors and cisplatin sensitivity of LUAD cells through negatively regulating GINS4. In conclusion, our findings demonstrated that GINS4 was overexpressed in LUAD and promoted the malignant behavior of LUAD cells. Moreover, miR-133a-3p could negatively regulate GINS4, thereby suppressing the malignant progression and increasing the cisplatin sensitivity of LUAD
研究领域:
肿瘤学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2021
·
IF 1.134
Qiu-Mei FENG,Li QIN,Peng ZHANG,Dan LI,Ming-Kai LIU,Po WANG
Building a precise, sensitive, and selective sensing platform is highly significant for microRNA (miRNA) detection. Herein, an innovative ratiometric electrochemical biosensor sensitized with carbon nanoparticles-functionalized SnS 2 nanoflowers ( [email protected] 2 NFs) and enzyme-assisted target recycling was explored for analysis of miRNA-21. In this strategy, the application of hierarchical [email protected] 2 NFs structure as electrode material not only enhanced the surface loading capability for subsequent reactions but also improved the interfacial electron transfer. After introducing enzyme-assisted target recycling, single-target miRNA-21 could initiate the cleavage of multiple capture DNA strands by duplex-specific nuclease (DSN), leading to numerous rest single-strand DNAs on the electrode. Two probes, methylene blue-labeled probe 1 (probe 1-MB) and ferrocene-labeled probe 2 (probe 2-Fc), could selectively and competitively hybridize with the capture DNA and the rest single-strand DNA, depending on the completeness of DNA hybridization. By measuring the peak current intensity ratio of Fc and MB, miRNA-21 was quantified in a wide concentration range from 1 fmol/L to 10 pmol/L, with a detection limit of 300 amol/L. Moreover, this sensing system showed good analytical performance in the presence of interfering sequences and complex matrices, illuminating excellent selectivity and practical application potential of the hierarchical [email protected] 2 NF sensing interface.
研究领域:
生物化学
·
纳米医学
·
分子诊断
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2022
·Vol. 147
·期 12
·2757-2763
·
IF 5.227
Xinying Zhang,Zhiwei Zhang,Chujing Yang,Ziheng Hu,Yingying Chen,Lingyan Feng
Signal amplification strategies increase the complexities of biosensors while improving the response signals. Herein, a novel electrochemical biosensor was developed based on a DNA hydrogel for sensitive analysis using microRNA-21 (miRNA-21) as a detection model. Poly C sequences combined with C–Ag(I)–C hydrogel formed a DNA hydrogel by the unique interaction between the cytosines and silver ions. Thus, with a three-way conjunction structure of DNA, this C–Ag(I)–C hydrogel was constructed as a novel biosensor for the detection of miRNAs. With the assistance of this hydrogel, numerous silver ions gathered around DNA strands, which would amplify the signal. Under these conditions, the silver ions produced distinct square wave voltammetry oxidation peak currents. This electrochemical biosensor we designed exhibited a great linear relationship for the logarithm of the concentration of miRNA-21 from 1 fM to 100 pM with a detection limit of 0.117 fM. Furthermore, our sensors were able to differentiate miRNA-21 from its homologous family with satisfactory responsiveness in the dilute bovine serum system.
研究领域:
生物传感技术
·
分子诊断
·
分析化学
·
纳米医学
✓ 已验证
DOI
PubMed
高影响因子
CELL BIOLOGY INTERNATIONAL
·
2018
·Vol. 42
·期 11
·1584-1593
·
IF 1.936
Bo Yu,Yanan Zhao,Hongli Zhang,Di Xie,Wei Nie,Kaiyao Shi
MicroRNA-143-3p (miR-143-3p) is involved in the initiation of inflammatory response and the progression of cardiovascular diseases. Myocardial hypertrophy is a common symptom in numerous cardiovascular diseases. In the current study, we attempted to demonstrate the role of miR-143-3p in the development of myocardial hypertrophy by focusing on its association with inflammation. Myocardial hypertrophy was induced by transverse aortic constriction (TAC) method in vivo and by H 2 O 2 administration in vitro. The expression status of miR-143-3p and downstream effectors were detected in animal heart tissues and H9c2 cells. Furthermore, the effect of miR-143-3p inhibition on H 2 O 2 -induced changes in ERK5/PPARδ/NF-κB axis was assessed. TAC induced oxidative stress and inflammation in rat heart tissues, which was associated with the increased expressions of miR-143-3p and p-ERK5. However, the up-regulated expression of miR-143-3p had no effect on the expression of ERK5, which was a direct target of miR-143-3p. The results of in vitro assays showed that H 2 O 2 administration increased the levels of miR-143-3p and p-EKR5 and induced the activation of NF-κB pathway. After the inhibition of miR-143-3p, the activation of EKR5 and NF-κB pathway was suppressed, whereas the expression of PPARδ was up-regulated. The current study demonstrated that miR-143-3p is crucial to the initiation of inflammatory response induced by myocardial hypertrophy. The activation of ERK5 following miR-143-3p up-regulation appears to be a complementary response to induce the subsequent anti-inflammatory signaling transduction, which needed further exploration.
研究领域:
心血管疾病
·
分子生物学
·
炎症
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Mo Wang,Huanshun Yin,Nannan Shen,Zhenning Xu,Bing Sun,Shiyun Ai
In this work, a photoelectrochemical (PEC) biosensor was fabricated for sensitive and specific detection of microRNA based on Bi 2 S 3 nanorods and enzymatic signal amplification . Using the catalytic effect of alkaline phosphatase on l -ascorbic acid 2-phosphate trisodium salt (AAP), ascorbic acid (AA) was in situ generated and used as electron donor . Based on this, a signal-on protocol was successively achieved for microRNAs detection due to the dependence of photocurrent response on the concentration of electron donor of AA. The results demonstrated that the photocurrent response enhanced with increasing the hybridized concentration of microRNA. Under the amplification of the immunogold labeled streptavidin (SA-AuNPs), a low detection limit of 1.67 fM was obtained. The fabricated biosensor showed good detection stability and specificity, and it could discriminate only one-base mismatched microRNA sequence. Moreover, the down-regulated expression of microRNA-21 in DF-1 chicken fibroblast cells infected with subgroup J avian leukemia virus (ALVs) was confirmed by the developed method, indicating that microRNA-21 might be a new biomarker for avian leukemia. This work opens a different perspective for microRNAs detection and early diagnose of avian leukemia.
研究领域:
生物传感器
·
分子生物学
·
病毒学
·
癌症研究
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano-Micro Letters
·
2019
·Vol. 11
·期 1
·1-16
·
IF 9.043
Xin Xiaofei,Du Xiaoqing,Xiao Qingqing,Azevedo Helena S.,He Wei,Yin Lifang
Nanosized cytotoxic drug could combat against the autophagy induced by the drug.
The drug nanorods enabled efficient intracellular delivery of the nucleic acid and the resultant autophagy inhibition in vitro and in vivo, which in turn sensitized the cancer cells to the anti-tumor nanorods.
研究领域:
药理学
·
纳米技术
·
癌症生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Shao Su,Wenfang Cao,Wei Liu,Zaiwei Lu,Dan Zhu,Jie Chao,Lixing Weng,Lihua Wang,Chunhai Fan,Lianhui Wang
The detection of microRNA plays an important role in early cancer diagnosis. Herein, a dual-mode electronic biosensor was developed for microRNA-21 (miRNA-21) detection based on gold nanoparticle-decorated MoS 2 nanosheet (AuNPs@MoS 2 ). A classical DNA “sandwich” structure was employed to construct MoS 2 -based electrochemical sensor , including capture DNA, target miRNA-21 and DNA-modified nanoprobe. [Fe(CN) 6 ] 3-/4- and [Ru(NH 3 ) 6 ] 3+ were selected as electrochemical indicators to monitor the preparation process and evaluate the performance of MoS 2 -based electrochemical biosensor by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV), respectively. Such MoS 2 -based biosensor exhibited excellent performance for miRNA-21 detection in the range from 10 fM to 1 nM with detection limit of 0.78 fM and 0.45 fM for DPV and EIS technique, respectively. Furthermore, the proposed MoS 2 -based biosensor displayed high selectivity and stability, which could be used to determine miRNA-21 in human serum samples with satisfactory results. All data suggested that such MoS 2 -based nanocomposite may be a potential candidate for biosensing ranging from nucleic acid to protein detection.
研究领域:
生物医学工程
·
分析化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 5
·1-10
·
IF 5.3
Yang Xiao,Li Chunguang,Xia Jianfei,Zhang Feifei,Wang Zonghua
A three-dimensional (3D) self-assembled AuNPs/Ti 3 C 2 MXene hydrogel (AuNPs/Ti 3 C 2 MXH) nanocomposite was prepared for the fabrication of a novel microRNA-122 electrochemical biosensor. The 3D hydrogel structure was gelated from two-dimensional MXene nanosheets with the assistance of graphite oxide and ethylenediamine. MXene hydrogels supported the in situ formation of Au nanoparticles (AuNPs) that predominantly exploring the (111) facet, and these AuNPs are utilized as carriers for hairpin DNA (hpDNA) probes, facilitating DNA hybridization. MXene acted as both a reductant and stabilizer, significantly improving the electrochemical signal. In addition, the conjugation of PAMAM dendrimer-encapsulated AuNPs and H-DNA worked as an ideal bridge to connect targets and efficient electrochemical tags, providing a high amplification efficiency for the sensing of microRNA-122. A linear relationship between the peak currents and the logarithm of the concentrations of microRNA-122 from 1.0 × 10 −2 to 1.0 × 10 2 fM (I = 1.642 + 0.312 lg c , R 2 = 0.9891), is obtained. The detection limit is 0.8 × 10 −2 fM (S/N = 3). The average recovery for human serum detection ranged from 97.32 to 101.4% (RSD < 5%). Graphical Abstract
研究领域:
生物化学
·
材料科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Long Chen,Guihua Hu,Bo Cui,Jinming Gao,Xiaofeng Gu,Haixin Cui,Zhanghua Zeng
The detection of microRNA (miRNA), a gene-regulating factor, is crucial for predicting plant growth and development. In this study, we successfully developed a fluorescent nanosensor using nanomaterials and molecular beacons for in situ miRNA detection in plant cells. The molecular beacons were loaded onto 15 nm gold nanoparticles , effectively protecting them from DNase I degradation. The nanosensor exhibits remarkable specificity and detection capability with a detection limit of 1.0 nM, while maintaining stability in plant nutrient solutions. It is readily absorbed and transported by plant through both the symplasmic pathway and apoplastic pathway. Furthermore, it efficiently converts miRNA signals into fluorescent signals, providing valuable insights into the spatial and temporal distribution of miRNA in plants. Although to achieve more accurate detection, the detection limit of the designed nanosensor still needs to be further improved, but this technique offers a convenient and efficient approach, serving as a powerful tool for studying the mechanisms and regulation in plants.
研究领域:
植物学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Biology-Basel
·
2024
·Vol. 13
·期 12
·960
·
IF 3.6
Kuo Yang,Jueyu Wang,Ying Zhang,Daizong Cui,Min Zhao
Simple SummaryThis study proposed a new method for extractingE. coliflagella, which served as a template for synthesizing palladium (Pd) nanowires. By improving the flagella extraction process, we successfully produced Pd nanowires with better structure and function, which could have important applications in technology and medicine. Traditional methods often led to impurities that interfered with nanomaterial synthesis, but our method provided a cleaner and more efficient way of producing high-quality nanowires. UsingE. coliflagella as a template offered environmental benefits, as it eliminated the need for toxic chemicals and reduced energy consumption. This eco-friendly approach was also safer and more reliable for practical applications. Our research also showed how the functional groups on the flagella surface helped form strong bonds with metals, improving the nanowire formation process. This study highlighted the potential of using biological templates for nanomaterial synthesis, contributing to the development of greener and more efficient technologies.In recent years, the use of bacterial flagella as biomimetic templates has gained increasing attention in nanomaterial synthesis due to their unique structural and functional properties. In this study, we optimized the flagella extraction method and achieved a high concentration of flagella solution. Flagella were isolated fromEscherichia coli. Surface characterization revealed that the flagella had abundant functional groups, such as amino and carboxyl groups, which can serve as nucleation sites for the controlled nucleation and growth of metal nanomaterials. Using bacterial flagella as a template, we synthesized one-dimensional palladium nanowires (Fla-Pd NWs). The results of morphological and phase analyses showed that the synthesized palladium nanoparticles were uniformly and densely distributed on the surface of the flagella. Moreover, the Fla-Pd nanowires exhibited superior electrocatalytic activity, which was applied to develop an electrochemical biosensor. This biosensor was used to detect the early breast cancer biomarker microRNA-21 and exhibited a linear range of 0.66–1.98 µmol/L and a detection limit of 0.78 µmol/L. The method demonstrated high selectivity and reusability, making it a promising strategy for early cancer diagnosis.
研究领域:
纳米材料
·
生物医学工程
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）