ACS Omega
·
2026
·
IF 5.2
Mingchen Zhang,Lei Jiang,Yu Liu,Ruiting Shen,Yiqiong Sun,Yang Zhou,Jiantao Zhang
Bacterial infections remain a major challenge in the treatment of chronic diabetic wounds, primarily due to hyperglycemic conditions that favor microbial proliferation. Conventional hydrogels incorporating physically entrapped antibiotics are often limited by burst release and inadequate long-term antibacterial efficacy. To address this, we developed a covalently grafted antibacterial hydrogel system based on neomycin sulfate (N) conjugated to sodium alginate (SA) to form SA-N. This modified polymer was further combined with poly(vinyl alcohol) (PVA) and cross-linked via boric acid to establish an interpenetrating network structure, denoted as PBN hydrogels. Successful covalent conjugation of neomycin was verified by FTIR and 1H NMR spectroscopy. Rheological analysis under alternating strain demonstrated the hydrogel’s rapid self-healing behavior, a critical feature for dynamic wound environments. The PBN hydrogel exhibited strong broad-spectrum antibacterial activity, achieving inhibition rates of 98.03% against Staphylococcus aureus and 93.19% against Escherichia coli. Moreover, in a diabetic mouse wound model, treatment with the PBN hydrogel resulted in accelerated wound closure, reaching 96.59% after 21 days. Collectively, the PBN hydrogel demonstrates excellent biocompatibility, sustained antibacterial performance, and promising therapeutic potential for the management of chronic diabetic wounds.
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高影响因子
ACS Applied Polymer Materials
·
2021
·
IF 4.089
Jiajun Chen,Zhinan Fan,Chi Zhang,Huatian Duan,Li-Juan Fan
To detect neomycin, one of the common antibiotics in the environment and in food and which may pose harm to human health, a fluorescence sensing system based on an amphiphilic conjugated polyelectrolyte (CPE) was constructed. The CPE, PFPE-COONa, with poly[fluorenyl-alt-p-phenyleneethynylene] (PFPE) as the backbone and carrying four pendant hydrophilic carboxylate groups and two long hydrophobic alkoxyl chains in each repeat unit, was successfully synthesized. The photophysical study found that PFPE-COONa emission was solvent dependent, displaying a 58 nm red shift in wavelength and a nearly 150-fold enhancement in intensity for the emission maximum, by varying the ratios of the mixed solvent. Based on an understanding of the photophysics, the sensing condition was optimization to dissolve PFPE-COONa in the THF/H2O mixed solvent with a ratio of 1:1. The sensing system displayed excellent sensing performance toward neomycin at a neutral pH value. The blue-to-green fluorescence color change of the polymer solution, which can be clearly observed by the naked eye, upon around 0.7 μM of neomycin and the limit of detection (LOD) was calculated to be 0.78 nM. In addition, other possible interferents had no significant influence on the detection of neomycin. The mechanism study confirmed that the polymer aggregation, which induced strong electrostatic static interaction between the carboxylate groups of high density in the polymer and the dense positively charged primary amino groups in neomycin, was the main reason for such a visually fluorescent color change. This study provides a general strategy for the future design of sensing systems for organic pollutants.
研究领域:
分析化学
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环境科学
·
材料科学
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高影响因子
Cell Death Discovery
·
2026
·
IF 10.4
Hao Wenli,Gao Song,Guo Suhan,Luo Jingjing,Li Siyu,Lu Junze,Jiang Lulu,Lu Jie,Wang Nan,Ran Aijia,Qian Xiaoyun,Gao Xia,Yu Chenjie,Cheng Cheng
Lipid metabolism is an important biological process that maintains the dynamic balance of several key functions, such as intracellular energy metabolism, signal transduction, and membrane remodeling. However, the role of lipid metabolism in auditory function and the underlying mechanisms remain unclear. Our results reveal that the neomycin exposure disrupts lipid metabolism in auditory system. We find that neomycin-induced hair cells (HC) damage leads to abnormal lipid droplets (LD) accumulation. Further research reveals that decreased YAP expression is a key factor that contributes to abnormal LD accumulation. In both in vivo and in vitro studies, Yap overexpression reduces abnormal LD accumulation and mitigated HC damage. To further investigate its downstream mechanisms, we perform a cross-analysis of Yap -related and lipid metabolism–related genes, identifying that Cox2 is a key downstream target of Yap that contributes to LD accumulation and HC damage. Our work provides clear evidence for the role of lipid metabolism in neomycin-induced hearing loss and elucidates underlying mechanism of Yap/Cox2 pathway. These findings provide new perspectives and avenues for the clinical treatment of sensorineural hearing loss.
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International Journal of Analytical Chemistry
·
2022
·
IF 1.698
Fanfan Zhang,Yangyang Chen,Dan Zhang,Yang Jia,Junsheng Meng,Lirong Jiang,Shengke Yang
A simple, rapid, and convenient method for the determination of neomycin based on the ion association method was proposed. In Britton–Robinson buffer solution, neomycin could react with Congo red to form an ionic association, which in turn reacted with dodecyl trimethyl ammonium bromide to form a ternary ionic association. The three were combined in a 1 : 1 : 1 ratio, which significantly enhanced the resonant Rayleigh scattering intensity at 468 nm. The obtained resonant Rayleigh scattering sensor showed a linear relationship with neomycin in the range of 0.07∼1 μg·mL−1. The limit of detection was 0.02 μg·mL−1, and the limit of quantification was 0.037 μg·mL−1. The experimental conditions were optimized. The method was verified based on the ICH rule. The established method could be applied to the analysis of the acceptable recovery rate of neomycin in powdered veterinary drugs.
研究领域:
分析化学
·
药物分析
·
光谱学
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高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2022
·Vol. 248
·期 8
·2157-2165
·
IF 3.498
Shi Qiaoqiao,Tao Chunxu,Kong Dezhao
Neomycin and lincomycin are widely used in the treatment of infections illnesses. However, the irrational and inappropriate of antibiotics contributes to threaten human health seriously. We developed a rapid and multiple detection strategy for trace amounts of neomycin (NEO) and lincomycin (LIN) in milk using Surface-Enhanced Raman Spectroscopy (SERS)-based lateral flow assay (LFA). The method used two SERS immunoprobes (a probe for NEO and a probe for LIN) were mixed and applied to the conjugate pad, and then, the NEO-OVA and LIN-OVA were mixed and coated on LFA as the only test line. Simultaneous quantitative detection of NEO and LIN was achieved by detecting the Raman intensity of captured immunoprobes on test line using a Raman spectrometer. Under optimized condition, NEO and LIN could be detected as low as 0.33 pg/mL and 0.29 pg/mL in 15 min with a sample volume of 100 μL. The practicality of the LFA in milk sample was also validated. The method is highly sensitive, accurate, and effective. It has a good chance to be employed for multiple antibiotics detection in food products.
研究领域:
分析化学
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食品安全
·
光谱学
·
生物技术
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高影响因子
SENSORS
·
2025
·Vol. 25
·期 13
·3920
·
IF 3.5
Yan Wu,Junshi Huang,Ni Tong,Qi Chen,Fang Peng,Muhua Liu,Jinhui Zhao,Shuanggen Huang
In the process of chicken breeding, there has been a great deal of abuse of antibiotics. Antibiotics can enter the human body along with the chicken meat, comprising a possible risk to human health. In this paper, principal component analysis (PCA)–linear discriminant analysis (LDA) was chosen to classify neomycin (NEO) and chloramphenicol (CAP) residues in chicken meat. A total of 400 chicken meat samples were used for the classification, of which 268 samples and 132 samples were used as the training sets and the test sets, respectively. The experimental condition of SERS spectrum collection was optimized, including the use of a gold colloid and active agent, and an improvement in the adsorption time. The optimal measurement conditions for the SERS spectra were an adsorption time of 4 min and the use of a 14th-generation gold colloid as the enhanced substrate without a surfactant. For three groups of different spectral preprocessing methods, the classification accuracies of PCA-LDA models for test sets were 78.79% for baseline correction, 84.85% for the second derivative and 100% for the second derivative combined with baseline correction. LDA was used to establish a classification model to realize the quick determination of NEO and CAP residues in chicken meat by SERS. The results showed that the characteristic peaks at 546 and 666 cm−1could be used to distinguish NEO and CAP residues in chicken meat. The classification model based on PCA-LDA had higher classification accuracy, sensitivity and specificity using a second derivative combined with baseline correction as the spectral preprocessing method, which shows that the SERS method based on PCA-LDA could be used to perform the classification of NEO and CAP residues in chicken meat quickly and effectively. It also verified the feasibility of PCA-LDA to effectively classify chicken meat samples into four types. This research method could provide a reference for the measurement of such antibiotic residues in chicken meat in the future.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Wen Zang,Minjie Peng,Yiyu Yang,Chenguang Zhang,Zhusheng Liu,Le Wang,Chaozhen Wang,Jie Lin,Tianxiang Chen,Yujie Zhang,Juan Li,Aiguo Wu
Neomycin sulfate (NEO) is a kind of aminoglycoside antibiotics. Because of its strong ototoxicity, nephrotoxicity and other side effects, its content in the body should be strictly monitored during use. In this paper, a rapid colorimetric detection method for NEO based on ultrasmall polyvinylpyrrolidone modified gold nanoparticles (PVP/Au NPs) with peroxidase-like activity was developed. Firstly, ultra small PVP/Au NPs with weak peroxidase-like activity were synthetized. When they were mixed with NEO, strong hydrogen bonds were formed between NEO and PVP, resulting in the aggregation of PVP/Au NPs, and the aggregated PVP/Au NPs showed stronger peroxidase-like activity. Therefore, rapid colorimetric detection of NEO was achieved by utilizing the enhanced peroxidase-like activity mechanism caused by the aggregation of ultra small PVP/Au NPs. The naked eye detection limit of this method is 50 nM. Within the range of 1 nM-300 nM, there was a good linear relationship between NEO concentration and the change in absorbance intensity of PVP/Au NPs-H 2 O 2 -TMB solution at 652 nm, with the regression curve of y = 0.0045x + 0.0525 (R 2 = 0.998), and the detection limit is 1 nM. In addition, this method was successfully applied to the detection of NEO in mouse serum. The recoveries were 104.4 % −107.6 % compared with HPLC assay results, indicating that this method for NEO detection based on PVP/Au NPs has great potential in actual detection of NEO in serum.
研究领域:
分析化学
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纳米技术
·
药物分析
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PubMed
高影响因子
Marine Drugs
·
2015
·Vol. 13
·期 4
·2465-2487
·
IF 2.853
Chang-Jing Wu,Le Yi,Cheng-Bin Cui,Chang-Wei Li,Nan Wang,Xiao Han
Introduction of neomycin-resistance into a marine-derived, wild-typePenicillium purpurogenumG59 resulted in activation of silent biosynthetic pathways for the secondary metabolite production. Upon treatment of G59 spores with neomycin and dimethyl sulfoxide (DMSO), a total of 56 mutants were obtained by single colony isolation. The acquired resistance of mutants to neomycin was testified by the resistance test. In contrast to the G59 strain, the EtOAc extracts of 28 mutants inhibited the human cancer K562 cells, indicating that the 28 mutants have acquired the capability to produce bioactive metabolites. HPLC-photodiode array detector (PDAD)-UV and HPLC-electron spray ionization (ESI)-MS analyses further indicated that diverse secondary metabolites have been newly produced in the bioactive mutant extracts. Followed isolation and characterization demonstrated that five bioactive secondary metabolites, curvularin (1), citrinin (2), penicitrinone A (3),erythro-23-O-methylneocyclocitrinol (4) and 22E-7α-methoxy-5α,6α-epoxyergosta-8(14),22-dien-3β-ol (5), were newly produced by a mutant, 4-30, compared to the G59 strain. All1–5were also not yet found in the secondary metabolites of other wild typeP. purpurogenumstrains. Compounds1–5inhibited human cancer K562, HL-60, HeLa and BGC-823 cells to varying extents. Both present bioassays and chemical investigations demonstrated that the introduction of neomycin-resistance into the marine-derived fungal G59 strain could activate silent secondary metabolite production. The present work not only extended the previous DMSO-mediated method for introducing drug-resistance in fungi both in DMSO concentrations and antibiotics, but also additionally exemplified effectiveness of this method for activating silent fungal secondary metabolites. This method could be applied to other fungal isolates to elicit their metabolic potentials to investigate secondary metabolites from silent biosynthetic pathways.Keywords:Penicillium purpurogenumG59;marine-derived fungus;neomycin resistance;DMSO;antitumor activity;fungal metabolite production
研究领域:
微生物学
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药物化学
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分子生物学
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PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2018
·
IF 2.831
Ying-chun Wan,Yan-jie Liu,Chen Liu,Hui-ting Ma,Hui-fang Yu,Jia-wei Kang,Chen-long Gao,Zhong-qiao Wu,Dan Zheng,Bin Lu
A new analytical method was developed to detect neomycin in complex biological samples using molecularly imprinted polymer to construct an optical sensor. Fluorescent neomycin-imprinted polymers (fMIPs) containing both imprinted cavity and boronate affinity site were synthesized on the surface of silica-modified quantum dots. The fMIPs exhibited high selectivity to neomycin by having two binding sites for the target analyte. Neomycin analogues (competing for imprinted cavity) and D-glucose (competing for the boronate affinity site) did not affect the selectivity of the fMIPs. When combined with a fluorescent microplate reader, the obtained fMIP sensor displayed a linear concentration-dependent fluorescence quenching in response to neomycin in the range of 2–1000 μg/L, with a limit of detection as 0.16 μg/L. The fMIP sensor was able to detect trace neomycin in biological samples accurately after simple sample pre-treatment. The sensitivity of the fMIP sensor was higher than HPLC equipped with a fluorescence detector. The fMIP sensor containing the doubly selective binding sites provides a selective, sensitive, accurate, and high through-put approach for neomycin monitoring.
研究领域:
分析化学
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生物医学工程
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药物分析
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Sawsan Dacrory
3D multifunctional scaffold has been designed based on Cs/SA/NS/NPHA. Nanoparticles hydroxyapatite (NPHA) was prepared via precipitation method of sodium dihydrogen phosphate in presence calcium chloride . Different ratios of Chitosan (CS)/Sodium Alginate (SA) were used to prepare Cs/SA scaffolds in presence of CaCl 2 as a cross linker . NPHA was incorporated in CS/SA scaffold and neomycin sulfate (NS) was added as an antimicrobial agent. The structure and surface morphology of the scaffolds were investigated via infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and thermal gravimetric analysis (TGA) techniques. Additionally, Antimicrobial activity of the scaffold has evaluated against Gram- negative and Gram- positive bacteria. The result showed promising antimicrobial activity against Bacillus subtilis , Staphylococcus aureus , Escherichia coli , Pseudomonas aeruginosa and Candida albicans . Furthermore, cytotoxicity against MG63 osteosarcoma cell and fibroblast normal cell line has investigated. The result showed anti-proliferative against MG63. DFT calculations and molecular docking were used to study the reactivity of the compounds. The results exhibited that Cs/SA/NS/NPHA is potent expected to be used in bone tissue regeneration .
研究领域:
生物材料学
·
药理学
·
组织工程学
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 2
·1-8
·
IF 5.705
Shi Qiaoqiao,Huang Jie,Sun Yaning,Deng Ruiguang,Teng Man,Li Qingmei,Yang Yanyan,Hu Xiaofei,Zhang Zhijun,Zhang Gaiping
The authors describe an ultrasensitive method for simultaneous detection of neomycin (NEO) and quinolones antibiotics (QNS). It is based on the use of (a) two immuno-nanoprobes (a probe for NEO and a probe for QNS), (b) surface-enhanced Raman scattering (SERS) detection, and (c), a portable lateral flow assay (LFA). The two probes consist of gold nanoparticles (AuNPs) conjugated to the Raman active molecule 4-aminothiophenol (PATP), and to monoclonal antibody against NEO (NEO mAb) or against NOR (NOR mAb). Quantitative detection of NEO and QNS was realized via SERS of the PATP-coated AuNPs captured in the test line of a LFA. Under optimized condition, the visual limits of LFA are 10 ng·mL −1 for NEO and 200 ng·mL −1 for NOR, and with LODs down to 0.37 pg·mL −1 and 0.55 pg·mL −1 by using SERS. The NEO test line is not interfered by the NEO analogues gentamycin, streptomycin and tobramycin, but the NOR test line suffers from different degrees of cross-reactivity (CR) to 12 common other QNS, the CRs ranging from 1.5% to 136%. The recoveries of NEO and NOR from spiked milk samples ranged between 86% and 121%, with relative standard deviations (RSD) from 3% to 6%. The method is highly sensitive, accurate and effective. It may be applied to simultaneous detection of NEO and 8 QNS, including NOR, enoxacin, ciprofloxacin, ofloxacin, fleroxacin, marbofloxacin, enrofloxacin, and pefloxacin.
Schematic of a lateral flow assay (LFA) based on an indirect competitive model. By using two test lines, the LFA can detect the neomycin and quinolones antibiotics simultaneously. Based on the surface-enhanced Raman scattering (SERS), the LFA shows high sensitivity to antibiotics with low limit of detection.
研究领域:
分析化学
·
纳米技术
·
药物分析
·
食品安全
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2018
·
IF 2.441
Mian Zu,Jinan Jiang,Hui Zhao,Shili Zhang,Yan Yan,Shiwei Qiu,Shuolong Yuan,Jiawei Han,Yue Zhang,Weiwei Guo,Shiming Yang
Irreversible hearing loss induced by aminoglycoside in human through local or systemic administration route negatively impacts quality of life. The aim of this work was to develop and validate an analytical method suitable for the detection and quantification of neomycin in cochlear perilymph of guinea pig after local application. The SupelMIP SPE column was used for the pre-treatment of matrix. Chromatographic separation was conducted by a reversed phase ODS column (100 × 2.1 mm, 3 μm) at 40 °C in gradient mode with 0.2‰ (v/v) HFBA in water and 0.2‰ (v/v) HFBA in acetonitrile as mobile phase , at a flow rate of 0.30 mL/min, with retention time of 3.50 and 3.62 min for internal standard tobramycin and analyte neomycin , respectively. The MS was performed with positive ionization mode, with data acquisition in Multi Reaction Monitor (MRM) mode. This method was proved to be specific, accurate (97.1–115% of nominal values) and precise (CV% < 15%). Calibration curves for matrix matched standard of neomycin ranged from 1.25 to 200 μg/mL, with LOD and LLOQ of 0.625 and 1.25 μg/mL in blank matrix. The matrix effect was corrected to (−0.1) - 1.33 by adding internal standard. The relative SPE recovery values were ≥98.9% in low, medium and high QC samples. Neomycin in matrix proved to be stable under room temperature - and −20 °C, or under three freeze-thawing cycles, or under processing as well. Finally, the proposed method was successfully applied to a toxicokinetics study of neomycin in perilymph after round window membrane (RWM) administration, which was in accordance with threshold shift of auditory brainstem response (ABR) test related to hearing loss.
研究领域:
药理学
·
分析化学
·
耳科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）