PHYTOCHEMICAL ANALYSIS
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2021
·Vol. 32
·期 6
·1074-1081
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IF 3.373
Yanfang Wu,Yindi Chu,Qinyu Yang,Mengjia Li,Miao Yu,Ruixue Deng,Kun Liu,Xinsheng Wang,Enguo Fan
Introduction Syringa pubescens Turcz. was reported to be abundant in the Funiu Mountains of Henan Province and can be used to treat hepatitis and cirrhosis. In order to develop and utilise the resource, a fast and simple technique to extract bioactive compounds is needed. Objectives Our aims were to provide an extraction technique of glycosides from S. pubescens and study the antioxidant activity of this material. Methods Box–Behnken design (BBD) was employed with three factors at three levels. The process parameters such as ethanol concentration ( X 1 ), temperature ( X 2 ), and solvent–solid ratio ( X 3 ) could significantly influence efficiency and yield of target compounds. High-performance liquid chromatography (HPLC) was used to determine the content of glycosides. DPPH (α,α-diphenyl-β-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and reducing power were used to evaluate the antioxidant activity of S. pubescens extract. Results The optimal parameters for the maximal extraction yield were obtained with ethanol concentration of 68%, temperature of 89°C, solvent–solid ratio of 46 mL/g, and time of 20 min. The S. pubescens extract showed strong antioxidant properties in vitro . Conclusion The findings indicated the potential application of solvothermal extraction method to extract bioactive compounds from S. pubescens Turcz. Furthermore, the S. pubescens extract could be used as an important resource of antioxidant activity.
研究领域:
植物化学
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药用植物学
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天然产物化学
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DOI
PubMed
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2022
·Vol. 66
·期 24
·2200455
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IF 6.575
Yuanjie Guo,Yu Ma,Liyuan Ma,Zixin Guo,Yingliang Xiao,Yunjun Liu,Jie Li,Shengnan Wang,Yixiang Liu
Scope This study assesses whether oleuropein prevents ovalbumin (OVA)-induced food allergy (FA) and investigates the underlying mechanisms. Methods and results A Balb/c FA mouse model is established and maintained for 7 weeks. The subjects are administered OVA by oral gavage to induce FA and supplemented with different oleuropein doses (1.00–20.00 mg kg −1 per day) to evaluate its preventative efficacy. The results indicate that oleuropein effectively alleviates OVA-induced allergy symptoms and promotes temperature elevation in sensitized mice. The secretion of serology-specific OVA-immunoglobulin (Ig)E, OVA-IgG, and histamine is inhibited in the sensitized mice. Oleuropein dramatically upregulates the expression of intestinal tight junction (TJ) proteins, regenerating gene (Reg) IIIγ, and interleukin (IL)-22, enhancing the physical and biochemical barrier function of the intestinal epithelium. Additionally, oleuropein improves the immune homeostasis of the intestinal epithelium by affecting the function of mucosal mast cells and regulatory T (Treg) cells. The disordered intestinal flora of the sensitized mice also improves after oleuropein administration. Conclusions These findings suggest that oleuropein prevents FA by enhancing intestinal epithelial barrier function and improving immune homeostasis and intestinal flora in sensitized mice. Therefore, diets rich in oleuropein should be recommended for people with FA.
研究领域:
免疫学
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胃肠病学
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营养学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Yueping You,Yi Wang,Meiyi Liang,Chenglin Wu,Zhiheng Huang,Jinlin Guo,Hulan Chen,Yu Fan
Given the limitations associated with conventional methods for oleuropein (OLE) analysis, such as their high cost, lengthy analysis time, and complex sample preparation procedures, this study proposed a novel colorimetric sensing assay based on nanozyme technology, with a specific focus on PCN-224-Pd composite materials. PCN-224 exhibited distinctive structural characteristics and possessed a substantial specific surface area, making it an ideal dispersion platform for Pd nanoparticles. This effectively enhanced the catalytic activity and stability of the nanozyme. Technologies such as transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), inductively coupled plasma optical emission spectrometry (ICP-OES), and fourier-transform infrared spectroscopy (FTIR) were employed to comprehensively characterize the morphology, structure, and composition of the synthesized PCN-224-Pd. Experimental characterizations revealed that PCN-224-Pd exhibited high affinity and the origin of PCN-224-Pd as oxidase mimics was likely dependent on the conversion of dissolved oxygen to 1 O 2 and •OH. Under optimal catalytic conditions, we developed a colorimetric sensing assay for oleuropein with an extended linear range (0.1–400 μg/mL) and an impressively low detection limit (9.7 ng/mL). These results indicated that the PCN-224-Pd based method held immense potential for practical applications as it could serve as a visual inspection technique for detecting oleuropein.
研究领域:
分析化学
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材料科学
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生物化学
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DOI
高影响因子
Antioxidants
·
2021
·Vol. 10
·期 11
·1697
·
IF 6.313
Shiling Feng,Chunyan Zhang,Tao Chen,Lijun Zhou,Yan Huang,Ming Yuan,Tian Li,Chunbang Ding
Oleuropein (OLE) is a secoiridoid glycoside that mainly exists in olives with multifaceted health benefits. The present study aimed to investigate the stress resistance and lifespan extension effects of OLE inCaenorhabditis elegans. The results showed that OLE could significantly prolong the lifespan ofC. elegansby 22.29%. Treatment with OLE also significantly increased the survival rates of worms against lethal heat shock and oxidative stress. Meanwhile, OLE supplementation increased the expression and activity of antioxidant enzymes and suppressed the generation of malondialdehyde in nematodes. In addition, the results from mutants implied that OLE might mediate longevity and stress resistance via DAF-16/FoxO, which played a vital role in the insulin/IGF-1 signaling (IIS) pathway. To further identify the molecular targets of OLE, mRNA level and loss-of-function mutants of IIS-associated genes were investigated. The data revealed that OLE activated IIS by down-regulating the upstream components,daf-2andage-1. Furthermore, another stress response and longevity pathway in parallel to DAF-16, SKN-1/Nrf2, was also shown to involve in OLE-induced beneficial effects. Collectively, these results provide the theoretical basis that OLE could enhance the stress resistance and increase the lifespan ofC. elegansthrough the IIS and SKN-1/Nrf2 signaling pathways.Keywords:oleuropein;Caenorhabditis elegans;longevity;stress resistance
研究领域:
分子生物学
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老年学
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药理学
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遗传学
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DOI
PubMed
高影响因子
Journal of Functional Foods
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2023
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IF 5.6
Shuyan Han,Hongmei Hou,Yiren Zhang,Zhongxiang Fang,Yun Li,Le Zhang,Yandan Wang,Shijia Zhang,Hui Zhang,Qingzhe Jin,Gangcheng Wu,Xingguo Wang
Naturally existing polyphenols in virgin olive oil possess significative biological activities and are potential nutraceutical therapeutic for breast cancer. In the present study, we investigated the anti-breast cancer activity of oleuropein and its hydrolysates, and further explored their molecular mechanisms. A similar cellular viability was observed in MCF-7 and MDA-MB-231 cells both treated with oleocanthal (3 μg/mL), hydroxytyrosol (30 μg/mL) and oleuropein (200 μg/mL) showed the same cellular viability, indicating oleocanthal structure may be the main structural component part of the physiological activity of oleuropein. Results showed that olive polyphenols produced reactive oxygen species and suppressed breast cancer cells. For flow cytometry analysis, the three polyphenols impacted the MCF-7 cell and MDA-MB-231 cycle, stagnated in the G1 phase and S phase, respectively, via reducing Cyclin D protein expression and promoting cell apoptosis. The pro-apoptosis, anti-proliferation capacity and delaying cell cycle of the three olive oil polyphenols could be achieved through the PI3K-AKT signaling pathway, mainly inhibited phosphorylation of AKT, PI3K protein.
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高影响因子
Analytical Methods
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2016
·Vol. 8
·期 7
·1532-1542
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IF 1.915
Lianfei Zhao,Hua Wang,Huiyan Gu,Lei Yang
Rapid and efficient solvent extraction of syringin and oleuropein from Syringa reticulata var. mandshurica branch bark was achieved using microwave-assisted ionic liquid–lithium salt pretreatment, followed by ultrasonic breakdown of the plant cell walls. The conditions for this novel sample treatment method, including microwave pretreatment time and power, ultrasound irradiation extraction time and power, types and concentrations of ionic liquid and lithium salt, and the liquid–solid ratio, were optimized using a dual response surface methodology. The proposed approach under optimal conditions was compared with conventional extraction methods. No degradation of syringin and oleuropein was observed in stability studies performed with a reference standard. The experimental results indicate that the proposed approach is a simple and efficient sample preparation technique that has strong potential for application in the field of sample analysis.
研究领域:
植物化学
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分析化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）