Materials Science & Engineering C-Materials for Biological Applications
·
2019
·
IF 4.959
Yu Zhu Yang,Na Xiao,Shi Gang Liu,Lei Han,Nian Bing Li,Hong Qun Luo
Intracellular pH level plays an important role in physiological and pathological processes. The development of nanoprobes for detecting in vivo pH levels is especially important for early diagnosis of disease. Therefore, we develop a hydrophilic carbon points (CDs) using quercetin and ethylenediamine as precursors to monitor intracellular pH. Under optimized conditions, the prepared CDs not only have uniform particle size and morphology, but also possess strong green fluorescence, photostability, and photoreversibility in water medium. The CDs exhibit pH-sensitive fluorescence effect under acidic and alkaline conditions, which is used to achieve “off-on-off” detection pH (from 3.5 to 13.5). Meanwhile, the pH-dependent mechanism is further investigated and explained, which is the fluorescence quenching caused by the pH-induced aggregation. Based on the pH-sensitive characteristics of CDs, it has been applied to the detection of aspartic acid and glutamic acid. More importantly, when applied to live cells, the pH-probe exhibits low cytotoxicity and high sensitivity, and is successfully used in intracellular pH fluorescence imaging. Consequently, this nanoprobe is expected to be used for real-time monitoring of intracellular pH level.
研究领域:
生物化学
·
纳米医学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Huangmei Zhou,Yu Zhao,Xihang Chen,Sanjun Zhang
pH is a fundamental physiological parameter related to myriad applications, therefore great efforts are continuously made to develop high performance pH indicators. Here, we exhaustively investigated the pH response of an acridine analog, 9-acridinecarboxylic acid with steady state absorption and fluorescence spectroscopies, picosecond resolved fluorescence spectroscopy, and femtosecond resolved transient absorption spectroscopy. The fluorescence intensity ratio (R 450/525 ) of 9-acridinecarboxylic acid showed a 7-fold change in the physiological pH range (pH ∼ 5–8), and it can be served as an excellent ratiometric and lifetime pH indicator. Analysis of the picosecond and femtosecond-resolved spectra combined with theoretical calculation revealed that 9-acridinecarboxylic acid existed in zwitterionic form at acidic condition with emission centered at 480 nm and lifetime of about 2.1 ns and anionic form at alkaline condition with emission centered at 430 nm and lifetime of about 6.6 ns. As pH decreased, the protonation of heterocyclic N atom resulted in the redshift of fluorescence peak and shortening of the fluorescence lifetime. Moreover, 9-acridinecarboxylic acid exhibited good selectivity, photostability and excellent biocompatibility and was successfully applied for intracellular pH imaging by ratiometric fluorescence.
研究领域:
分析化学
·
生物物理学
·
光谱学
·
荧光成像
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Lingling Li,Yang Li,Yijing Dang,Tingting Chen,Ao Zhang,Chunyong Ding,Zhiai Xu
Intracellular pH is a key physiological factor for controlling the activities and functions of cells and lysosome is a vital subcellular organelle. Thus, developing a novel lysosome-targeting fluorescence probe for selective and sensitive detection of lysosomal pH in living cells is very important. In this work, we synthesized a series of fluorescence probes based on imidazole-fused benzothiadiazole . The optical properties of these probes were easily adjusted by modifying the substituents with different electron-withdrawing/donating ability in imidazole moiety. All of them showed acid-response and decreased fluorescence intensity during pH values changing from 4.0 to 8.5. The introduction of morpholine group allowed them to specifically respond to the changes of lysosomal pH. Among them, probe MIBTAA possessed a suitable p K a value (5.3) and showed good linear response to pH (R 2 = 0.9918) with red emission when pH changed from 4.4 to 5.6. The probe was successfully applied for monitoring pH variation in living cells induced by proton-pump inhibitor Baf-A1 and chloroquine , indicating its great potential for pH imaging in biological applications.
研究领域:
生物化学
·
细胞生物学
·
医学影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Jie Zhou,Limin Zhang,Yang Tian
To develop in vivo monitoring meter for pH measurements is still the bottleneck for understanding the role of pH plays in the brain diseases. In this work, a selective and sensitive electrochemical pH meter was developed for real-time ratiometric monitoring of pH in different regions of rat brains upon ischemia. First, 1,2-naphthoquinone (1,2-NQ) was employed and optimized as a selective pH recognition element to establish a 2H+/2e– approach over a wide range of pH from 5.8 to 8.0. The pH meter demonstrated remarkable selectivity toward pH detection against metal ions, amino acids, reactive oxygen species, and other biological species in the brain. Meanwhile, an inner reference, 6-(ferrocenyl)hexanethiol (FcHT), was selected as a built-in correction to avoid the environmental effect through coimmobilization with 1,2-NQ. In addition, three-dimensional gold nanoleaves were electrodeposited onto the electrode surface to amplify the signal by ∼4.0-fold and the measurement was achieved down to 0.07 pH. Finally, combined with the microelectrode technique, the microelectrochemical pH meter was directly implanted into brain regions including the striatum, hippocampus, and cortex and successfully applied in real-time monitoring of pH values in these regions of brain followed by global cerebral ischemia. The results demonstrated that pH values were estimated to 7.21 ± 0.05, 7.13 ± 0.09, and 7.27 ± 0.06 in the striatum, hippocampus, and cortex in the rat brains, respectively, in normal conditions. However, pH decreased to 6.75 ± 0.07 and 6.52 ± 0.03 in the striatum and hippocampus, upon global cerebral ischemia, while a negligible pH change was obtained in the cortex.
研究领域:
神经科学
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2014
·Vol. 14
·期 10
·1495-1504
·
IF 3.65
Pan Zhang,Xiaoxue Song,Weijun Tong,Changyou Gao
The dual-labeled microcapsules via nanoparticle/polymer assembly based on polyamine–salt aggregates can be fabricated for the ratiometric intracellular pH sensing. After deposition of SiO 2 nanoparticles on the poly(allylamine hydrochloride)/multivalent anionic salt aggregates followed by silicic acid treatment, the generated microcapsules are stable in a wide pH range (3.0 ∼ 8.0). pH sensitive dye and pH insensitive dye are simultaneously labeled on the capsules, which enable the ratiometric pH sensing. Due to the rough and positively charged surface, the microcapsules can be internalized by several kinds of cells naturally. Real-time measurement of intracellular pH in several living cells shows that the capsules are all located in acidic organelles after being taken up. Furthermore, the negatively charged DNA and dyes can be easily encapsulated into the capsules via charge interaction. The microcapsules with combination of localized pH sensing and drug loading abilities have many advantages, such as following the real-time transportation and processing of the carriers in cells.
研究领域:
材料科学
·
生物医学工程
·
分析化学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2018
·Vol. 16
·期 48
·9402-9408
·
IF 3.423
Pan Li,Zhe Chen,Yishun Huang,Jing Li,Fan Xiao,Shiyao Zhai,Zhiming Wang,Xuanjun Zhang,Leilei Tian
A new DNA-based fluorescent probe, which is a hybrid molecule of an i-motif forming sequence (IFS) and mono-functionalized tetraphenylethene (TPE), has been synthesized and investigated. A distinct pH-responsive aggregation-induced emission (AIE) effect has been observed from this hybrid molecule, i.e. the fluorescence of TPE will be turned on when the IFS part folds up under acidic conditions. According to the fact that a hybrid molecule with a relatively rigid structure shows a more obvious AIE effect, and whose nano-sized aggregates are formed at a high concentration, we assume that the solubility of the hybrid molecule in water will be reduced due to IFS folding, resulting in aggregation and the resultant AIE effect. Finally, due to its excellent pH responsiveness, this DNA-based probe employing an AIEgen has been applied in monitoring intracellular pH.
研究领域:
生物化学
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 12
·2049
·
IF 5.719
Tianyu Gao,Jinwei Lin,Leilei Xu,Jianguo Guan
In living bodies, pH values, which are precisely regulated and closely associated with diseased cells, can act as an efficient biologically intrinsic indicator for future intelligent biomedicine microsystems. In this work, we have developed flask-like carbonaceous nanomotors (FCNMs), via loading Fe3O4nanoparticles (NPs) into a cavity, which exhibit a self-adaptive feature to a specific physiological pH by virtue of the pH-dependent dual enzyme-like activities of Fe3O4NPs. Specifically, the peroxidase-like activity of Fe3O4NPs in an acidic pH range, and the catalase-like activity in a near neutral and alkaline pH range, determine the products in the motion system (•OH, ions and O2), whose diffusions from the inner to the outside of the flask result in fluid movement providing the driving force for the movement of the FCNMs. Correspondingly, changes of the product concentrations and species in the physiological pH range (4.4–7.4) result, firstly, in velocity decrease and, then, with increase in pH, increase of the FCNMs occurs. Thanks to the non-linear velocity responsiveness, the FCNMs show intriguing pH taxis towards 6.8 (generally corresponding to the physiological pH in tumor microenvironments), where a maximum velocity appears. Furthermore, the superparamagnetic feature of the Fe3O4NPs simultaneously endows the FCNMs with the abilities to be magnetic-oriented and easily separated. This work could significantly increase the possibility of nanomotors for targeted therapy of tumors and next-generation biotechnological applications.Keywords:nanomotors;pH responsiveness;Fe3O4nanoparticles;dual enzyme-like activities;physiological pH chemotaxis
研究领域:
生物医学工程
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2016
·
IF 2.101
Feng-Fei Pang,Sen Li,Wen-Quan Sun,Guo-Zhi Han
We report the reversible conductivity and morphology modulation of PEDOT:PSS composites based on pH. Through studying effects of pH on the morphology and conductivity of PEDOT:PSS composites, it was found that different pH of PEDOT:PSS composites solution will result in different morphology of PEDOT:PSS composites film . At the same time, conductivity of the film also changed accordingly. With the increase of pH from 3 to 11, the microstructure of PEDOT:PSS film reversibly changed from a continuous network to non-continuous clusters, and the corresponding conductivity decreased from 0.62 S/cm to 0.42 S/cm. Furthermore, the mechanism was innovatively investigated by Raman spectra , along with UV–Vis spectra and SEM .
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2020
·
IF 4.65
Bingxin Xie,Lei Lei,Jinghua Xu,Shiqing Xu
An pH-sensitive citric acid coated Ce/Tb:KGd 2 F 7 nanocrystals with a broad detection range from 3 to 10 is established. Under the 254 nm excitation, the Tb 3+ emission intensity originated from Ce 3+ → Gd 3+ →Tb 3+ decreases gradually with the increase of pH, while its intensity almost keeps unchanged in pH 3 to 6 and increases in pH 6 to 10 under the 311 nm excitation, which activates the ratiometric pH sensing with high sensitivity.
研究领域:
材料科学
·
纳米技术
·
传感器
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 49
·30971-30979
·
IF 3.361
Huadong Zhang,Zhongyi Yan,Zhizhou Yang,Jinshui Yao,Qiuhong Mu,Dan Peng,Hui Zhao
A novel silicone resin (SR-OH) containing phenolic hydroxyl (Ph–OH) groups was designed and synthesized via co-hydrolysis/condensation and catalytic hydrogenation. During the curing process, the cross-linking degree of the resin was further increased by the Si–O–Ph bonds formed by the reaction of the Ph–OH and terminal Si–OH groups. Thermogravimetric analysis (TGA) showed that the cured resin product exhibited excellent thermal and thermo-oxidative stability, which was much higher than that of a typical methyl phenyl silicone resin (SR-Ph). The temperature at which 5% weight loss occurs (Td5) was up to 606 °C (nitrogen) and 542 °C (air), and its char yield at 800 °C was 91.1% and 85.3% in nitrogen and air, respectively. The significant improvement in thermal stability was mainly attributed to the formation of Si–O–Ph bonds which not only increases the cross-linking degree of the resin but also significantly prevents degradation by the ‘back-biting’ and oxidative cleavage.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Xiulian Yin,Peng Liu,Xuechao Xu,Jianming Pan,Xin Li,Xiangheng Niu
In recent years, a growing number of nanosized materials that can mimic the catalytic characteristics of some natural enzymes have experienced a period of rapid development. Among these nanomaterials defined as ‘nanozymes’, peroxidase-mimicking materials draw intensive interest because of their potential applications in various fields, including biosensing, medicine, and environmental engineering. Nonetheless, most of currently explored peroxidase nanozymes exhibit favorable activity only in acidic environments, which greatly restricts their use especially in biochemical analysis. Developing peroxidase mimics with good catalytic performance in a broad pH range is highly desired and still challenging. Herein, we report a vitriolization strategy to broaden the catalytic pH scope of peroxidase-like CoFe 2 O 4 . The vitriolization process endows the nanomaterial with sustainable acidity on its surface. As a result, the obtained peroxidase nanozyme (SO 4 2− /CoFe 2 O 4 ) exhibits good catalytic activity in neutral or even weak alkaline solutions. This provides us a one-step assay for colorimetric glucose detection in one pot at physiological pH via combining the proposed peroxidase mimic with glucose oxidase, which shows advantages of easier operation and faster detection while offering comparable analytical performance.
研究领域:
生物化学
·
医学检测
·
纳米材料科学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 22
·10849-10856
·
IF 3.3
Hufeng Fang,Shan Xu,Jinhong Gong,Lidan Tang,Xiaomei He,Ying Lin,Hao Yang,Kun Yan,Dan Su,Yujing Leng
Metabolic alkalosis, the most common acid–base disorder, is related to significantly increased mortality in hospitalized patients. Hunting for pH sensors lying in the neutral to alkaline pH range still remains challenging and in demand. Herein, we engineered a colorimetric/fluorescent method for monitoring alkalic pH based on a phenylboronic acid-containing probe (DDPB). In this approach, the dicyanoisophorone group of DDPB acted as an optical signal moiety, while the boronic acid acted as the latent pH response site. It was exactly deciphered that the reversible pH monitoring principle is based on the deprotonation/protonation mechanism and intramolecular charge transfer (ICT) strategy via1H NMR and DFT calculations. This approach presented a pH-sensitive response over pH values ranging from 7.00 to 10.23 (pKa = 9.33) with excellent reversibility. We further explored its application in sensing pH on a paper substrate for visual and rapid detection. In addition, it was successfully applied to measure pH variations in HK-2 cells when the cells underwent alkalosis. This work extends the application of optical probes based on boronic acid under alkaline pH conditions, and provides a new strategy for the design of alkaline pH probes.
研究领域:
化学
·
材料科学
·
生物医学工程
·
细胞生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）