LANGMUIR
·
2022
·
IF 4.331
Chaonan Li,Ya Wang,Zihao Wang,Xiaogang Li
Although paraquat is a widely used herbicide, it is toxic to humans if ingested or absorbed through an open wound. To improve the safety of paraquat, a new formulation of paraquat based on photoresponsive polymers was exploited. Photoresponsive β-cyclodextrin polymer microspheres (AZO-CD) were synthesized via a host–guest interaction between β-cyclodextrin and azobenzene. AZO-CD were characterized by Fourier transform infrared spectrometry, circular dichroism, ultraviolet (UV) spectrophotometry, and thermogravimetric analysis, and their photoresponsiveness was also evaluated. AZO-CD were used to load paraquat, which yielded photoresponsive paraquat-loaded microspheres. For the paraquat-loaded microspheres, irradiation with UV light or sunlight induced the isomerization of azobenzene into the cis form. Then, the cis-azobenzene was liberated from the cavities of the β-cyclodextrin. The paraquat-loaded microspheres released paraquat continuously over time. Furthermore, under UV light, the herbicidal capacity of the paraquat-loaded microspheres against barnyard grass was comparable to that of free paraquat at the same dose. Our findings show that loading paraquat into AZO-CD provides a safe and environmentally friendly herbicide formulation.
研究领域:
农业化学
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环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomed Research International
·
2022
·Vol. 2022
·期 1
·1254824
·
IF 3.246
Jielun Yu,Lichun Zhang,Xiaoshuang Li,Kaixuan Lv,Shiyu Sun,Weihua Wu,Lifeng Ping,Guifang Guo,Wei Tan,Shoudong Guo,Kezhou Wang,Aihua Zhao,Nana Yang
Background . This study was designed to investigate differences in biochemical parameters between mouse and humans after paraquat (PQ) poisoning and develop a suitable animal model for studying organ damage after PQ poisoning. The prognostic factors of PQ-poisoned patients were further analyzed. Methods . Thirty C57BL/6J mice were randomly divided into five groups (control, sham, and 3 PQ doses), and the mouse model was established by intragastric administration of PQ. Physiological indexes such as the body weight, mental state, and mortality rate were observed. Biochemical parameters were analyzed 24 h after PQ poisoning. We also performed a retrospective analysis of clinical data from 29 patients with PQ poisoning admitted to the Emergency Department of the Affiliated Hospital of Taishan Medical College between April 2016 and February 2018. Biochemical parameters were compared between the mouse model and patients with PQ poisoning. Results . In the PQ poisoning mouse model, the lethal dose group PQ360 showed remarkable increases in serum levels of potassium (K + ), carbon dioxide (CO 2 ), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) compared with the nonlethal dose PQ100 and PQ200 groups. The biochemical results of the patients showed that K + and Cl - levels were significantly reduced in the death group compared to the survival group. Levels of ALT, AST, blood urea nitrogen (BUN), and amylase were higher, and the neutrophil-to-lymphocyte ratio (NLR) was increased in the death group compared with the survival group. Conclusions . The combination of age, PQ dosage, K + , Cl - , BUN, ALT, AST, amylase, and NLR can be used to more accurately predict the outcome of patients with PQ poisoning. C57 mice are an appropriate animal model to study liver and kidney functions following PQ exposure.
研究领域:
毒理学
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医学
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实验动物学
✓ 已验证
DOI
高影响因子
Frontiers in Cell and Developmental Biology
·
2021
·
IF 6.684
Sun Yan-Li,Wang Xue-Lin,Yang Lei-Lei,Ge Zhao-Jia,Zhao Yong,Luo Shi-Ming,Shen Wei,Sun Qing-Yuan,Yin Shen
Paraquat (PQ) is a widely used non-selective and oxidizing herbicide in farmland, orchards, flower nursery, and grassland. Overuse of PQ will accumulate in the body and affect the reproduction in mammals. In this study, we found that PQ could reduce the female fertility by oral administration for 21 days in mice. PQ exposure could impair the nuclear maturation by perturbing the spindle assembly and kinetochore–microtubule attachment to cause the misaligned chromosomes during meiosis. In the meantime, PQ exposure disturbed the mitochondrial distribution and enhanced the level of reactive oxygen species and early apoptosis, which thereby deteriorated the early embryo development. Also, PQ administration could cause some changes in epigenetic modifications such as the level of H3K9me2 and H3K27me3. Therefore, PQ administration reduces the female fertility by impairing the nuclear and cytoplasmic maturation of oocytes in mice.
研究领域:
生殖生物学
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毒理学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 14
·1-13
·
IF 2.8
Hou Enhui,Kong Zhiguo,Wu Jianfeng,Wang Hairui,Nie Ping,Lu Ming,Chang Limin
Mxenes, a general term for a novel class of two-dimensional carbon–nitrogen compounds, which attracted extraordinary attention because of their unique electrochemical property. In this paper, an Ag-TiO 2 /MXene composite material was prepared for paraquat (PQ) detection by differential pulse voltammetry (DPV). It was found that the detection limit reached as low as 0.01 μM and linear range was from 0.05 to 15 μM (split into two sections: 0.05 to 1 μM and 1 μM to 15 μM). The Ag-TiO 2 /MXene composite electrode material exhibits excellent anti-interference properties to the species including the ions such as Cu 2+ , Na + , Ca 2+ , Zn 2+ , Cl − , SO 4 2− and biomass molecules such as glucose, ascorbic acid and uric acid. In the presence of organophosphorus pesticides, the electrode also showed good anti-interference. The excellent paraquat detection capability of the Ag–TiO 2 /MXene electrode material should be attributed to the synergistic effect obtained by decorating Ag and TiO 2 on MXene. In conclusion, the functionalization of MXenes through surface-modification provided a feasible route for ultrasensitive electrochemical sensors construction.
研究领域:
材料科学
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电化学
·
环境监测
✓ 已验证
产品关联
DOI
高影响因子
BIOLOGICAL & PHARMACEUTICAL BULLETIN
·
2015
·Vol. 38
·期 3
·470-475
·
IF 1.828
Xianqin Wang,Meiling Zhang,Jianshe Ma,Yuan Zhang,Guangliang Hong,Fa Sun,Guanyang Lin,Lufeng Hu
Numerous people die of paraquat (PQ) poisoning every year in the world. Although several studies regarding paraquat (PQ) poisoning have been conducted, the metabolic changes in plasma remain unknown. In this study, the metabolomics of 15 PQ poisoned patients with plasma PQ concentrations in excess of 0.1 µg/mL and 16 healthy volunteers were investigated. The plasma samples were evaluated through the use of gas chromatography-mass spectrometry (GC/MS) and analyzed by partial least-squares discriminant analysis (PLS-DA). Based on the metabolomics data, a support vector machine (SVM) discrimination model was developed. The results showed the plasma levels of urea, glucose oxime and L-phenylalanine decreased and cholesterol increased in PQ poisoned patients in comparison to healthy volunteers. The SVM discrimination model was developed, and performed with a high degree of accuracy, to distinguish PQ poisoned patients from healthy volunteers. In conclusion, metabolic pathways including the urea cycle, and amino acid, glucose, and cholesterol metabolism were impaired after PQ poisoning. An SVM discrimination model, based on metabolomics data, was established and may become a new powerful tool for the diagnosis of PQ poisoning.
研究领域:
毒理学
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医学
·
代谢组学
✓ 已验证
DOI
PubMed
高影响因子
IONICS
·
2025
·
IF 2.6
Cao Lin-An,Li Xiao-Qin,Li Yi-Qian,Huo Yun-Fei,Chen Lu
Paraquat (PQ) is a highly toxic herbicide but widely used in agricultural production. Highly sensitive detection of trace PQ is vital and challenging. Herein, two-dimensional electronically conductive metal–organic framework (2D EC-MOF) Ni 3 (HHTP) 2 (HHTP: 2,3,6,7,10,11-hexahydroxytriphenylene) is in situ constructed on the carbon paper and employed as an electrochemical sensor for PQ detection for the first time. The Ni 3 (HHTP) 2 electrochemical sensor exhibits a high detection sensitivity (175 μA·μM −1 ·cm −2 ), low detection limit (4.4 nM), good reproducibility, and strong anti-interference ability. In addition, the sensor shows reliable performance in the detection of PQ in real samples including apples, cucumbers, tomatoes, and pears (detection recovery is 95.4–104.4%). Furthermore, zeta potential test (a method which can obtain zeta potential value on the particle surface) and density functional theory (DFT) calculation confirm that the strong electrostatic interactions between active sites and PQ are conducive to the high-sensitivity detection of PQ. The accession of 2D EC-MOF to PQ electrochemical sensor provides new ideas for sensor design and broadens the scope of EC-MOF’s applications. Graphical abstract
研究领域:
分析化学
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材料科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
Experimental and Therapeutic Medicine
·
2019
·Vol. 18
·期 5
·3643-3649
·
IF 1.448
Cui Huang,Jing Ma,Bai‑Xiang Li,Yan Sun
Wingless (Wnt) signaling regulates the proliferation and differentiation of midbrain dopamine (DA) neurons. Paraquat (PQ) and maneb (MB) are environmental pollutants that can be used to model Parkinson's disease (PD) in rodents. A previous study demonstrated that developmental exposure to PQ and MB affects the expression of Wnt1, Wnt5a, nuclear receptor‑related factor 1 (NURR1) and tyrosine hydroxylase (TH). However, how Wnt signaling regulates these developmental factors in vitro is yet to be determined. To explore this, SH‑SY5Y cells were exposed to PQ and MB. The results of the current study indicated that exposure to PQ and MB decreased Wnt1, β‑catenin, NURR1 and TH levels and increased Wnt5a levels. Furthermore, Wnt1 silencing has the same effect as exposure to PQ and MB. Additionally, the neurotoxicity induced by PQ and MB is more severe in siWnt1‑SH‑SY5Y cells compared with normal SH‑SY5Y cells. Therefore, Wnt1 may serve an important role in regulating developmental DA factors, and may be a candidate gene for PD diagnosis or gene therapy.
研究领域:
神经科学
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毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Wenwen Chen,Chen Li,Zhi Yu,Ying Song,Xiubing Zhang,Dejiang Ni,De Zhang,Pei Liang
Paraquat is a highly effective herbicide and widely used in agricultural production. However, paraquat residue is harmful for human health and can cause irreversible hazard. Thus, it is crucial for monitoring of paraquat residues. In this paper, an efficient SERS platform based on cactus-inspired nanoparticles is proposed for sensitive detection of paraquat. The cactus-liked nanoparticles obtained from one-pot stepwise reduction method possess multiple spiny structures and can produce abundant hot spots, resulting in remarkable SERS performance. SEM, TEM, UV–vis and Raman tests were conducted to characterize and optimize the morphology of cactus-liked nanoparticles under different preparation conditions. The synthesis mechanism and corresponding parameters influence mechanism of cactus-liked nanoparticles were explored in detail. Optimized substrate exhibited a high sensitivity with the detectable concentration of crystal violet (CV) down to 10 -9 M and an excellent reproducibility proved by SERS mapping. Furthermore, it behaved good linear relationship with a correlation coefficient (R 2 ) of 96.89% between Raman intensities and concentrations of paraquat, which indicates the SERS substrate prepared with cactus-liked nanoparticles could offer a great potential for identification of paraquat.
研究领域:
农业科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MEDICAL SCIENCE MONITOR
·
2021
·
IF 2.649
Feiya Jiang,Tongtong Wang,Sha Li,Yu Jiang,Zhuo Chen,Wen Liua
Background Fluorofenidone (AKF-PD) is an anti-fibrotic small-molecule compound. Its mechanism of action on paraquat (PQ)-induced pulmonary fibrosis is still unclear. Material/Methods Forty-eight SD rats were divided into 4 groups: control group, PQ group, PQ+AKF-PD group, and AKF-PD group. The pathological changes of lung tissues were observed by Masson and HE staining. The UPLC-QTOF-MS analysis was performed to detect the differences in metabolites among groups, then the possible mechanisms of the anti-pulmonary fibrosis effects of fluorofenidone were further revealed by network pharmacology analysis. Biological methods were used to verify the results of the network pharmacology analysis. Results The results showed that fluorofenidone treatment significantly alleviated paraquat-induced pulmonary fibrosis. Metabolomics analysis showed that 18 metabolites were disordered in the serum of paraquat-poisoned rats, of which 13 were restored following fluorofenidone treatment. Network pharmacology analysis showed that the drug screened a total of 12 targets and mainly involved multiple signaling pathways and metabolic pathways to jointly exert anti-pulmonary fibrosis effects. Autophagy is the main pathway of fluorofenidone in treatment pulmonary fibrosis. The western blot results showed that fluorofenidone upregulated the expression of LC3-II/I and E-cadherin, and downregulated the expression of p62, α-SMA, and TGF-β1, which validated that fluorofenidone could inhibit the development of paraquat-induced pulmonary fibrosis by increasing autophagy. Conclusions In conclusion, metabolomics combined with network pharmacology research strategy revealed that fluorofenidone has a multi-target and multi-path mechanism of action in the treatment of pulmonary fibrosis.
研究领域:
医学
·
药理学
·
生物信息学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Ying Song,Jinlei Chen,Xiaobin Yang,De Zhang,Yanqiu Zou,Dejiang Ni,Jiaming Ye,Zhi Yu,Qiang Chen,Shangzhong Jin,Pei Liang
Fe 3 O 4 magnetic nanoparticles with a particle size of ∼ 250 nm were synthesized by the thermal solvent method, and then modified with polyethyleneimine (PEI) as the intermediate layer on the Fe 3 O 4 surface to form ultra-thin polymer interlayer to replace the traditional carbon shell and silicon shell interlayer. The seeding of Fe 3 O 4 magnetic particles was completed thanks to the strong positive charge of the amino group of PEI-adsorbed 3–5 nm colloidal gold on the surface of magnetic beads, and the addition of silver nitrate and reducing agent to the magnetic microsphere enabled the silver shell to be coated on the surface of the magnetic microspheres almost without affecting their magnetism. Moreover, the effects of the additive amount of colloidal gold, silver nitrate and ultrasonic time on the magnetic nanoparticles were systematically studied. Morphology and characterization analysis showed that the prepared Fe 3 O 4 @Ag magnetic nanoparticles (MNPs) had uniform particle size, good dispersibility, strong magnetic response, and powerful SERS performance. Furthermore, the SERS performance of the composite nanometer material was investigated by using 4-aminophenol and pesticide paraquat as active targets, with the molecular concentration down to 10 -8 M and 10 -10 M, respectively, confirming that the functional composite had a good SERS activity and Fe 3 O 4 magnetic core.
研究领域:
材料科学
·
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Hui Fang,Xin Zhang,Shang Jie Zhang,Luo Liu,Yong Mei Zhao,Hai Jun Xu
In our study, we introduced a rapid, low-cost and nondestructive ‘surface spray’ method to achieve the quantitative detection of the pesticide residues on fruits skins. Taking advantage of the high enhancement ability of the surface-enhanced Raman spectroscopy (SERS) of the optimized Ag nanoparticles colloid, the limit of detection (LOD) of paraquat (PQ) on the peels of pears and apples could reach the order of magnitude of 10 −9 M, much lower than the maximum residue limit of 0.05 mg/kg (∼10 −4 M) in a large number of countries including the USA and China. The quantitative detection of PQ was achieved as well. This opens a new avenue for advancing the applications of SERS technique in the fields of food safety, drug security, and environment monitoring.
研究领域:
食品安全
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环境监测
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Tianyu Qiu,Cheng Liu,Yan Ding,Lixiang Wang,Yuhui Liu,Yang Sun,Zhengsheng Mao,Peng Chen,Hao Sun,Feng Chen,Yue Cao
Applying antioxidant coating materials to prepare surface-enhanced Raman spectroscopy (SERS) sensing substrates can effectively enhance the sensitivity and stability for the analysis of molecules. In this study, we have leveraged SERS to develop an innovative sensor for the swift identification of Paraquat (PQ), enabling on-site detection of this herbicide. The newly devised sensor distinguishes itself through its exceptional oxidation resistance. This resistance is attributed to the physical properties of the nanoparticles, specifically the silver shell coating and loading on the molybdenum disulfide (MoS 2 ). By the creation of “hot spots” of the composite nanoparticles (Ag@AuBPs on flower-like MoS 2 ), the kit achieves a remarkably low detection limit as low as 1.0 × 10 −10 M for Paraquat in lake water, soil, and clothing samples, allowing for rapid and direct identification of PQ in complex environments.
研究领域:
分析化学
·
毒理学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）