FOOD CHEMISTRY
·
2025
·
IF 9.8
Qianqian Lu,Lingling Guo,Xinxin Xu,Hua Kuang,Liqiang Liu,Chuanlai Xu,Maozhong Sun
The mass production and use of organophosphorus pesticides (OPs) have led to a threat to human health. Therefore, establishing a sensitive, rapid, and high-throughput detection method is of great importance. In this study, computer-aided molecular design was firstly applied to design the specific haptens of phorate (PHO), fenthion (FEN), and profenofos (PRO), and high-performance monoclonal antibodies against PHO, FEN, and PRO were prepared. On this basis, a multiplex fluorescent microsphere immunochromatographic assay (FM-ICA) was developed for simultaneous detection of three OPs in cowpea and orange samples. The quantitative detection ranges of PHO, FEN, and PRO were 9.039–140.129, 6.686–108.613, and 5.215–71.479 μg/kg, respectively, in cowpeas; and 7.120–121.566, 3.592–92.185, and 3.579–60.081 μg/kg, respectively, in oranges. The results of recovery and real sample testing showed that the multiplex FM-ICA was rapid and accurate, making it suitable for the on-site testing of a large number of samples.
研究领域:
分析化学
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环境科学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Ri-Sheng Chen,Jian-Cheng Yuan,Chong-Lan Luo,Yu Wang,Zi-Jian Chen,Ze-Shan Lin,Zhen-Lin Xu
Profenofos (PFF) is a commonly used organophosphorus insecticide that requires strict monitoring due to its potential environmental, ecological, and human health risks originating from residues in soil and water systems, as well as accumulation in crops. In this study, a novel monoclonal antibody (mAb) specific to PFF was prepared for the first time and the recognition mechanism was investigated through molecular simulation. Subsequently, a mAb-based colloidal gold immunochromatographic assay (GICA) was developed for the rapid screening of PFF in fruit and vegetable samples. The mAb exhibited an IC 50 value of 12.9 ng/mL, and limit of detection (LOD) of 4.6 ng/mL, respectively in indirect competitive immunosorbent enzyme-linked immunosorbent assay (ic-ELISA). After optimization, the developed GICA exhibited a visual limit of detection (vLOD) of 20 ng/mL and a quantitative of detection (qLOD) of 5.2 ng/mL, with a linear range from 10.0 to 83.8 ng/mL. Good correlation was observed between the results of GICA and standard Gas Chromatography-Tandem Mass Spectrometry (GC-MS/MS) in matrix and recovery test. The developed GICA can be used for rapid sample detection within 15 min, which is an excellent tool for screening PFF in foods and environmental samples.
研究领域:
环境科学
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食品科学
·
免疫学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Pengcheng Yan,Xingwang Zhang,Yu Zhang,Pengfei Zheng,Guanyu Wu,Xiaojie She,Zhao Mo,Jintao Dong,Keqiang Xu,Henan Li
Given the health risks posed by profenofos (PFF) pesticide residues in aquatic environments, photoelectrochemical (PEC) aptasensors provide a viable solution for efficient detection, with accelerated photoelectrode interfacial carrier migration being critical for high-sensitivity sensing. A variable-valence Ce 3+ /Ce 4+ ionic bridge-modified carbon nitride (CN) material was engineered for PEC aptasensing applications. As a rare-earth species, Ce 3+ /Ce 4+ features incompletely filled 4 f and unoccupied 5d orbitals, which induce electron trap states in the CN matrix. These states restructure CN’s band configuration, narrow its band gap, and enhance photogenerated charge carrier generation. Moreover, Ce 3+ /Ce 4+ ionic bridge serves as an efficient electron transport medium, boosting charge migration and diffusion across the CN surface, thus elevating the material’s overall PEC performance. The resulting Ce 3+ /Ce 4+ /CN-based PEC aptasensor achieved sensitive PFF detection, with a linear range of 1–30000 fM and a detection limit of 0.33 fM (S/N = 3). It also exhibited strong selectivity, stability, reproducibility, and a low relative standard deviation (RSD=4.89% < 5%). This strategy enables effective aquatic PFF residue determination, while the valence-reversible ion-doped CN holds promise for broader rapid PEC analysis of organophosphorus pesticides.
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DOI
高影响因子
FOOD CONTROL
·
2011
·
IF 2.812
Jianwei Dong,Na Gao,Yuan Peng,Chun Guo,Zhiqiang Lv,Ying Wang,Caihong Zhou,Baoan Ning,Ming Liu,Zhixian Gao
A surface plasmon resonance (SPR) sensor that uses molecularly imprinted polymer (MIP) ultrathin film as sensing material was developed for detecting profenofos in tap water . The MIP ultrathin film was anchored on a gold chip by surface-initiated radical polymerization . The gold surface was first modified by 11-mercaptoundecanoic acid to form a self-assembled monolayer (SAM). To easily graft 2,2-azobis (2-amidinopropane) hydrochloride onto the SAM, the carboxyl of SAM was activated using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N -hydroxysuccinimide. Then, the MIP film was synthesized by thermal-initiated polymerization using methacrylic acid as functional monomer and trimethylolpropane trimethacrylate as cross-linker. On the basis of this principle, two methods for ultrathin film preparation were studied and the most suitable technique was adopted. The prepared ultrathin film was characterized by atomic force microscopy . The MIP-SPR sensor was used to detect profenofos in tap water spiked with 0.001–0.1 μg mL −1 of the insecticide. The sensor showed high sensitivity, selectivity and good stability. The recoveries and repeatability (RSD, n = 4) of the sensor were 89.81–100.99% and 3.69–5.76%, respectively, while the detection limit based on a signal-to-noise ratio of 3 (S/N = 3) was 3.6 × 10 −4 μg mL −1 .
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
ANALYST
·
2022
·Vol. 147
·期 18
·4105-4115
·
IF 5.227
Xin Tan,Wancui Xie,Qi Jia,Fangyuan Zhao,Wei Wu,Qingli Yang,Xiudan Hou
In this work, a simple, sensitive and selective colorimetric method was established for the detection of profenofos. Firstly, novel flower-shaped AuPtRh trimetallic nanospheres were synthesized via a simple one-pot method, and had outstanding peroxidase catalytic activity. AuPtRh nanospheres with a great specific surface area were linked with an aptamer via Au–S and Pt–S bonds to specifically recognize profenofos. A graphene oxide grafted stainless-steel mesh (SSM–GO) was prepared to be a carrier and the aptamer–AuPtRh was nonspecifically adsorbed on the surface of SSM–GO, which was to be the capture probe for the detection of profenofos in real samples. They were characterized and confirmed by transmission electron microscopy, atomic force microscopy, etc. Through the investigation of the catalytic performance on the basis of the Michaelis equation, the Vmax of AuPtRh nanospheres was 22.27 × 10−8 M s−1, and Km was 0.6632 mM, which indicated that the affinity of AuPtRh nanospheres was relatively higher than that of horseradish peroxidase and Au NPs. In the presence of profenofos, the aptamer–AuPtRh would specifically combine with profenofos, which would further detach from SSM–GO. Then, it was introduced into the 3,3′,5,5′-tetramethylbenzidine/H2O2 (TMB/H2O2) system to form blue oxTMB. The linear range of this colorimetric biosensor was 1–300 ng L−1 and the limit of detection was 0.725 ng L−1. It also had good recovery and anti-interference ability in real samples, which provided a new strategy for the rapid detection of pesticides.
研究领域:
分析化学
·
纳米技术
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环境科学
·
生物传感器
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xiaojie Shi,Huimin Liu,Mei Zhang,Fengzhen Yang,Jiansen Li,Yemin Guo,Xia Sun
An ultrasensitive aptasensor based on luminol-hydrogen peroxide (H 2 O 2 ) electrochemiluminescence (ECL) system was proposed for determination of profenofos. AuNPs decorated with MWCNTs ( [email protected] ) were used to provide a favorable microenvironment for luminol loading and improve the ECL signal due to their good biological activity, excellent electrical conductivity and high specific surface area. Gold and silver core-shell nanoparticles ( [email protected] ) could act as effective catalyzer to accelerate H 2 O 2 decomposition to produce various intermediate reactive oxygen species for signal amplification and thus increased the ECL signal again. Under optimal conditions, the proposed ECL aptasensor obtained an extremely low detection limit of 5.32 fg/mL (S/N = 3) with a good detection range from 100 fg/mL to 1 μg/mL. Moreover, the proposed aptasensor had excellent specificity, outstanding repeatability and favorable stability. Finally, the proposed ECL aptasensor was used for the detection of profenofos in real samples and the recoveries ranged from 94% to 102%. This strategy could provide a good reference for developing effective aptasensors for detection of other small molecules and toxins.
研究领域:
分析化学
·
环境科学
·
纳米技术
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Jiansen Li,Fengzhen Yang,Xiaofeng Chen,Honggang Fang,Chuanyun Zha,Jingcheng Huang,Xia Sun,Mohamed Bedair Mohamed Ahmed,Yemin Guo,Yuan Liu
Due to the diversification and complexity of organophosphorus pesticide residues brings great challenges to the detection work. Therefore, we developed a dual-ratiometric electrochemical aptasensor that could detect malathion (MAL) and profenofos (PRO) simultaneously. In this study, metal ions , hairpin-tetrahedral DNA nanostructures (HP-TDN) and nanocomposites were used as signal tracers, sensing framework and signal amplification strategy respectively to develop the aptasensor. Thionine (Thi) labeled HP-TDN (HP-TDNThi) provided specific binding sites for assembling Pb2+ labeled MAL aptamer (Pb2+-APT1) and Cd2+ labeled PRO aptamer (Cd2+-APT2). When the target pesticides were present, Pb2+-APT1 and Cd2+-APT2 were dissociated from the hairpin complementary strand of HP-TDNThi, resulting in reduced oxidation currents of Pb 2+ (I Pb 2+ ) and Cd 2+ (I Cd 2+ ), respectively, while the oxidation currents of Thi (I Thi ) remained unchanged. Thus, I Pb 2+ /I Thi and I Cd 2+ /I Thi oxidation current ratios were used to quantify MAL and PRO, respectively. In addition, the gold nanoparticles (AuNPs) encapsulated in the zeolitic imidazolate framework (ZIF-8) nanocomposites ( [email protected] ) greatly increased the catch of HP-TDN, thereby amplifying the detection signal. The rigid three-dimensional structure of HP-TDN could reduce the steric hindrance effect on the electrode surface , which could greatly improve the recognition efficiency of the aptasensor for the pesticide. Under the optimal conditions, the detection limits of the HP-TDN aptasensor for MAL and PRO were 4.3 pg mL-1 and 13.3 pg mL-1, respectively. Our work proposed a new approach to fabricating a high-performance aptasensor for simultaneous detection of multiple organophosphorus pesticides, opening a new avenue for the development of simultaneous detection sensors in the field of food safety and environmental monitoring.
研究领域:
分析化学
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环境科学
·
食品安全
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纳米技术
·
生物传感器
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Liancheng Fang,Yujing Zhou,Tianming Chen,Yuehan Geng,Zijing Li,Wei Zha,Taozhong Shi,Rimao Hua
Profenofos residues in the environment pose a high risk to mammals and non-target organisms. In this study, the biodegradation and detoxification of profenofos in an efficient degrading strain, Cupriavidus nantongensis X1 T , was investigated. Strain X1 T could degrade 88.82 % of 20 mg/L profenofos in 48 h. The optimum temperature and inoculation amount of strain X1 T for the degradation of profenofos were 30–37 °C and 20 % ( V /V), respectively. Metabolic pathway analysis showed that strain X1 T could degrade both profenofos and its main metabolite 4-bromo-2-chlorophenol. Metabolite toxicity analysis results showed that dehalogenation was the main detoxification step in profenofos biodegradation. The key gene and enzyme for profenofos degradation in strain X1 T were also explored. RT-qPCR shows that organophosphorus hydrolase (OpdB) was the key enzyme to control the hydrolysis process in strain X1 T . The purified enzyme OpdB in vitro had the same degradation characteristics as strain X1 T . Divalent metal cations could significantly enhance the hydrolysis activity of strain X1 T and enzyme OpdB. Meanwhile, strain X1 T could degrade 60.89 % of 20 mg/L profenofos in actual field soil within 72 h. This study provides an efficient biological resource for the remediation of profenofos residual pollution in the environment.
研究领域:
环境微生物学
·
生物修复
·
酶学
·
生态毒理学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2012
·Vol. 137
·期 5
·1252-1258
·
IF 4.23
Na Gao,Jianwei Dong,Ming Liu,Baoan Ning,Chaonan Cheng,Chun Guo,Caihong Zhou,Yuan Peng,Jialei Bai,Zhixian Gao
A quartz crystal microbalance (QCM) sensor based on molecularly imprinted ultra-thin films was developed for detecting profenofos in real samples. Films prepared by physical entrapment (MIP-A) and in situself-assembly (MIP-B) were compared. The results indicated that the best sensing signal was obtained through the in situself-assembly method. The QCM sensor chip was pretreated with 11-mercaptoundecanoic acid (MUA) to form a self-assembled monolayer (SAM), and then polymer films were immobilized directly on the SAM using surface-initiated radical polymerization. In this paper, all detection experiments were taken in air. The reaction was processed in solution, and the electrode was washed with deionized water and dried with N2 before QCM measurement. The film was characterized by a scanning electron microscope (SEM), AC impedance and cyclic voltammetry. Analysis of the QCM response in the presence of different concentrations of profenofos showed a good linear correlation during 1.0 × 10−8 to 1.0 × 10−5 mg mL−1 (y = 5log x + 42.5, R = 0.9960) and 1.0 × 10−5 to 1.0 × 10−3 mg mL−1 (y = 25.86log x + 146, R = 0.9959), respectively. The MIP-QCM sensor was used to detect profenofos in tap water, and showed good recovery and repeatability.
研究领域:
分析化学
·
环境科学
·
材料科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 4
·239
·
IF 5.4
Li Guangyan,Cheng Xiu,Zhu Minnan,Tian Yayang,Chen Linfeng
To address the need for rapid detection of profenofos (PFF) residues, we developed a self-cascaded colorimetric sensor using the bifunctional nanozyme Ce-UiO-66@His, prepared by doping histidine (His) into Ce-UiO-66. The introduction of His optimized the valence state distribution of cerium and increased the concentration of oxygen vacancies, thereby endowing Ce-UiO-66@His with enhanced phosphatase-like and laccase-like activities. Crucially, these two enzymes can efficiently catalyze in sequence under the same catalytic conditions, thereby achieving an integrated self-cascaded catalytic reaction of PFF. Specifically, the phosphatase-like activity hydrolyzes PFF to generate 4-bromo-2-chlorophenol, which is then oxidized by the laccase-like activity and coupled with 4-aminoantipyrine to form a red quinone-imine dye with strong absorbance. This self-cascaded, dual-recognition system provides exceptional selectivity and high sensitivity for PFF, with a detection limit of 0.71 µg/mL. Furthermore, by integrating smartphones and RGB recognition, a portable photo-colorimetric method was developed that realizes rapid and visual quantification of PFF. This work not only presents a new strategy for rapid monitoring of PFF residues but also offers insights into designing efficient biomimetic self-cascaded sensing systems. Graphical abstract
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PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Jia Wei,Yi Zhang,Wangui Peng,Weihong Huang,Yunfei Cao,Wenming Yang,Wanzhen Xu
Pesticide residues in food pose significant risks to human health, necessitating accurate monitoring methods. To address this, a lattice distortion strategy-based highly sensitive photoelectrochemical (PEC) sensor was developed for profenofos (PFF) detection. Substitution of Bi 3 + with Co 2+ in the Bi 2 MoO 6 lattice induced enhanced lattice distortions and oxygen vacancies, reducing the bandgap and dramatically improving visible-light absorption and charge transfer efficiency. These modifications synergistically boosted PEC performance, with 4 % Co-doped Bi 2 MoO 6 achieving optimal results, including a reduced narrowed bandgap (2.28 eV) and a significantly enhanced photocurrent density compared to pristine Bi 2 MoO 6 . The sensor exhibited a broad linear detection range (0.5 mg L −1 to 0.01 ng L −1 ) and an ultralow detection limit of 3.3 pg L −1 , demonstrating excellent sensitivity and selectivity in real food samples. This work presents an innovative efficient, non-toxic, and eco-friendly tool for pesticide residue detection, paving new avenues for food safety monitoring and public health protection.
研究领域:
食品科学
·
环境科学
·
分析化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Wenze Li,Weichen You,Xiaofan Yang,Yi Wang,Rimao Hua,Saraschandra Naraginti,Meiqing Zhu
As a typical chiral organophosphate insecticide, the enantiomer of profenofos exhibits significant bioselective toxicity in the environment. This study investigates the enantioselective effects of chiral profenofos and 2-Bromo-4-chlorophenol on BSA. Multispectral analysis confirmed that S/R -profenofos and 2-Bromo-4-chlorophenol alterd the microenvironment of BSA, decreasing the α-helices content from 53.6 % to 52.9 %, 53.0 %, and 52.8 %. The binding constants K a for the interaction of S/R -profenofos and 2-Bromo-4-chlorophenol with BSA were 4.70, 4.66 and 4.23 × 10 4 M −1 , respectively. The LC 50 of S/R -profenofos and 2-Bromo-4-chlorophenol for zebrafish were about 0.670, 0.739 and 0.954 mg/L, while the acetylcholinesterase inhibition rate were 86.64 %, 81.21 % and 73.64 %, respectively. The toxicity levels follows the pattern of S -profenofos> R -profenofos>2-Bromo-4-chlorophenol, which was also confirmed by the molecular docking and cell experiments. This study evaluated the interaction mechanism between chiral pesticides and model proteins at the molecular level, providing a new perspective on the food safety risk of chiral pesticides.
研究领域:
环境科学
·
分子生物学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）