MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 4
·1-8
·
IF 5.7
Wu Ning-Ning,Chen Lin-Ge,Xiao Min-Zhi,Yuan Rong-Yao,Wang Hai-Bo
A fluorescent method is described for trypsin determination through the strong electrostatic interactions between cationic polyelectrolytes and single-stranded DNA (ssDNA) templated Au nanoclusters (AuNCs). The ssDNA-AuNCs display improved fluorescence emission with excitation/emission maxima at 280/475 nm after being incorporated with poly(diallyldimethylammonium chloride) (PDDA). Fluorescent enhancement is mainly attributed to the electrostatic interactions occurring between PDDA and ssDNA templates. This can make the conformation of the ssDNA templates to change. Thus, it offers a better microenvironment for stabilizing and protecting ssDNA-AuNCs, and results in fluorescence emission enhancement. By using protamine as a model, the method is employed for the determination of trypsin. The assay enables trypsin to be determined with good sensitivity and a linear response ranging from 5 ng⋅mL −1 to 60 ng⋅mL −1 with a 1.5 ng⋅mL −1 limit of detection. It is also extended to determine the trypsin contents in human’s serum samples with recoveries between 98.7% and 103.5% with relative standard deviations (RSDs) between 3.5% and 4.8%. Graphical abstract A novel fluorescent strategy has been developed for of trypsin determination by using protamine mediated fluorescent enhancement of DNA templated Au nanoclusters.
研究领域:
分析化学
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 1
·263
·
IF 4.2
Shuji Liu,Yue Zhang,Yihui Chen,Yongchang Su,Bei Chen,Yin Wang,Min Xu,Kun Qiao,Shuigen Li,Zhiyu Liu
Protamine is a cationic peptide derived from fish sperm and has several important functional properties: antibacterial properties, acting as a carrier for injectable insulin and as a heparin antagonist, combatting fatigue, etc. Thus, it has been widely used in medicinal applications and food products. CulturedTakifugu flavidusis a type of pufferfish with a delicious taste that is popular in China, and its production is increasing significantly. Therefore, protamine was extracted via acid extraction from the sperm ofTakifugu flavidusand further isolated and purified via sephadex gel chromatography, ion exchange chromatography, and desalination chromatography. Furthermore, the physicochemical properties of protamine were investigated. The results showed that the sperm of the culturedT. flaviduswere non-toxic, and the extracted and purified protamine had high contents of arginine (36.90%) and lysine (27.02%), respectively. The secondary structure of protamine was mainly β-folded and irregularly curled. Additionally, protamine exhibited high thermal stability with a denaturation temperature of 176 °C. This study would provide a theoretical basis for the structural analysis, bioactivity, and resource development of pufferfish protamine and help to promote the development of the pufferfish industry.
研究领域:
生物化学
·
医药学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 36
·16537-16542
·
IF 3.591
Yingxi Zhou,Hongbo Jiang,Yuting Wang,Song Zhao,Lianzhe Hu,Yan Zhang
In this research, a distyryl-anthracene derivative (DSAI) with two quaternary ammonium groups was synthesized. It emitted strong yellow-green fluorescence after aggregating with heparin through electrostatic attraction. In the presence of protamine, the fluorescence of DSAI remarkably quenched because of the greater affinity between heparin and protamine. Based on the change of fluorescence signal, a highly sensitive and selective method is successfully constructed to detect heparin and protamine. The DSAI sensor toward heparin showed a broad linear range (0.1 to 1.1 μg mL−1) and a low detection limit (down to 5.64 ng mL−1). The linear detection for protamine was in a wide range (0.1 to 1.7 μg mL−1) with the detection limit of 5.20 ng mL−1. Moreover, DSAI was successfully applied to detect heparin and protamine in complicated goat serum samples with satisfactory results. This simple and ultrasensitive biosensor has potential applications in diagnostics, therapeutics and biological research.
研究领域:
生物化学
·
分析化学
·
医学诊断
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 56
·期 6
·4396-4406
·
IF 3.553
Zhang Kena,Ma Ge,Wang Haiqing,Liang Zhenda,Zhou Li,Yan Bing
The dynamics of the nucleolus in living cells are important in understanding cellular behavior and malignant transformation. However, construction of a fluorescent probe for long-term monitoring of nucleolus is significantly constrained by the cost, cellular barriers, cytotoxicity, and photobleaching. Herein, a one-pot microwave-assisted synthesis is developed for facile and quick preparation (domestic microwave oven, 7.5 min) of fluorescent carbon dots (CDs) for nucleolus imaging and tracking merely by adding protamine (PTM) into the precursor solution. The as-prepared carbon dots (CD-PTMs) feature photostability, biocompatibility, multicolor emission, and fluorescence enhancement upon binding with RNA. Significantly, CD-PTMs exhibit the capability of crossing both the cytoplasm and nuclear membranes, and located at the nucleolus for long-term (at least 12 h) imaging studies. Moreover, CD-PTMs are demonstrated to be capable of serving as efficient nanocarriers for in vitro transfection applications by taking advantage of the positively charged surface PTM derivative, showing the potential of CD-PTMs as multifunctional nanoprobes for the bioapplications. Graphical abstract
研究领域:
材料科学
·
纳米技术
·
细胞生物学
·
生物医学工程
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Pirah Ayaz,Xi Liu,Zhi Chen,Yuyun Yu,Javaria Khayaban E. Erum,Tingting Sun,Hongyan Diao,Xiangdong Liu
The immobilization of antibacterial peptides (APs) onto silk material substrates remains challenging due to the scarcity of green, low-cost, and efficient methods for covalent bonding between these two proteins. The present work proposed a novel strategy for covalently grafting APs onto silk fiber surfaces for antibacterial purposes. Firstly, silk fiber surfaces were grafted with glutamine (Gln) through a cure-induced esterification reaction, the AP protamine (PM) was then covalently grafted via transglutaminase (TGase)-catalyzed cross-linking onto the fiber surfaces. FTIR, SEM, XPS investigations verified the successful attachment of PM and the covalent connection between the PM and the fiber surfaces. The modified silk fabric showed bacteria reduction rates higher than 99.99 % against S. aureus and E. coli , and maintained its antibacterial properties after 20 washing cycles, highlighting a desirable antibacterial activity and considerable durability. Moreover, the modifications did not sacrifice the appearances and wearing comfortabilities of silk fabric, but even led to significant enhancement in the moisture transfer properties. This strategy, based on naturally occurring amino acid, AP and enzyme, could not only be an attractive alternative for real-world application in the silk industry but also could be extended to the modification of other protein-based materials.
研究领域:
材料科学
·
生物化学
·
纺织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Zichun Qi,Nana Pan,Dongxue Han,Jiahua He,Jin-ao Li,Luqi Yang,Xiaojuan Wang,Fang Huang
Though the heparin-protamine complex (HP complex) is a crucial system utilized in clinical settings, the metabolic pathways of this complex remain inadequately understood. Herein, the enzymatic degradation of the heparin-protamine complex by trypsin and its broader implications were investigated. By utilizing fluorescent gold nanoclusters liganded with the HP complex (AuNCs-HP complex), we observed significant morphological and spectral changes during enzymatic degradation. Experiments showed that AuNCs-HP complex could be degraded and cleaved into small fragments by trypsin. Moreover, the AuNCs-HP complex demonstrated its potential as a highly sensitive spectral sensing platform, enabling precise measurement of trypsin activity with an outstanding detection limit (0.34 ng mL −1 ). Additionally, we explored its utility for specific tumor cell detection, focusing on lung adenocarcinoma cells, and successfully identified their presence through distinctive fluorescence changes. These remarkable findings not only contribute valuable insights into targeted degradation systems but also offer promising opportunities for cancer biomarker detection. The AuNCs-HP complex serves as an innovative tool for real-time trypsin activity monitoring, paving the way for advanced biomedical applications.
研究领域:
生物化学
·
生物医学工程
·
癌症研究
·
纳米技术
·
光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Guangxin Feng,Shuwei Tang,Yuxin Gang,Mingyong Zeng,Haohao Wu
Nanostructured iron (III) compounds are promising candidates for iron fortification applications due to their good solubility, bioavailability, and redox inertia. The current study synthesized ferric oxyhydroxide nanoparticles (FeONPs) based on chondroitin sulfate (ChS) and its nanocomposites with protamine sulfate (PS) or chitosan (CS) in neutral aqueous solution under ambient conditions, and evaluated their iron availability to polarized human intestinal epithelial (Caco-2) cells. Ultraviolet-visible spectroscopy, dynamic light scattering, transmission electron microscopy and energy-dispersive X-ray spectroscopy showed that ChS-FeONPs were wave-like anionic particles where FeONPs attached on the polysaccharide chains and PS/ChS-FeONPs and CS/ChS-FeONPs were irregular anionic nanoparticles where FeONPs scattered across the entire region. The calcein-fluorescence-quenching assay in polarized Caco-2 cells showed good iron uptake from ChS-FeONPs, PS/ChS-FeONPs and CS/ChS-FeONPs mainly via endocytosis, with the latter two exhibiting better iron absorption. Overall, our study provides a more facile and greener alternative route to synthesize the bioavailable nano-sized iron.
研究领域:
营养学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 45
·26765-26770
·
IF 3.119
Yiping Chen,Zuan Lin,Chenfang Miao,Qianqian Cai,Fenglan Li,Zongfu Zheng,Xinhua Lin,Yanjie Zheng,Shaohuang Weng
The development of a simple detection strategy for trypsin (Try) is urgent, and is ascribed to the diagnostic value of Try in several diseases. Herein, a facile but effective fluorescence strategy for Try was developed based on the protamine (Pro)-induced aggregation of carbon quantum dots (CQDs). The fluorescence of negatively charged CQDs was quenched with Pro due to the assembly of CQDs and Pro (CQDs/Pro) through electrostatic interaction. However, the highly positively charged Pro, which is rich in basic arginine residues, was preferred to be hydrolyzed by Try. Try can induce the deaggregation of CQDs/Pro, thereby enabling the release of CQDs to restore the fluorescence intensity. Thus, the use of CQDs/Pro as a testing platform will be employed as a “turn-on” method for Try. In addition, the fluorescence-resuming response was proportional to Try, ranging from 25 ng mL−1 to 500 ng mL−1 with a limit of detection (LOD) of 8.08 ng mL−1. This “turn-on” fluorescence assay for Try was label-free, convenient, and relatively free of interference from coexisting substances. Actual applications for Try monitoring and trypsin inhibitor screening also illustrated the considerable prospect of CQDs in the clinical field, combined with the superiority of the simple mixing operation.
研究领域:
生物化学
·
医学诊断
·
纳米材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 29
·3586-3591
·
IF 3.1
Li Zhang,Jiyan Huang,Mixue Chen,Hongmei Huang,Yi Xiao,Ronghua Yang,Youyu Zhang,Xiaoxiao He,Kemin Wang
Amphiphilic aggregation-induced emission (AIE) molecules show superior potential for fabricating novel ultrasmall nanoprobes. Here, an anionic dipyridyl tetraphenylethene (TPE) derivative is rationally designed and a super-small self-assembled AIEgen nanoprobe (TPE-2Py-SO3NaNPs, ca. 2.48 nm) is thus conveniently constructed for the supersensitive detection of protamine and trypsin. In HEPES/DMSO solution (8 : 2, v/v, pH = 7.4), negatively charged TPE-2Py-SO3NaNPs exhibited an AIE effect in the presence of positively charged protamine, presenting a fluorescence enhancement at 498 nm together with a large Stokes shift of 150 nm and a low detection limit of 8.0 ng mL−1. In addition, the in situ formed TPE-2Py-SO3Na/protamine nanocomposite can be dissociated by trypsin due to the highly selective degradation of protamine via enzymatic hydrolysis, achieving a detection limit for trypsin as low as 5.0 ng mL−1.
研究领域:
生物化学
·
分析化学
·
医学检测
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Rui Jiang,Song Zhao,Langkun Chen,Maoyu Zhao,Wenjing Qi,Wensheng Fu,Lianzhe Hu,Yan Zhang
In this research, a new DSA (Distyryl-anthracene) derivative with four carboxyl groups was designed and synthesized. This molecule exhibits aggregation-induced emission property (AIE). With the AIE character, a convenient and sensitive fluorescent probe for the detection of protamine , heparin and heparinase has been developed. The protamine can directly induce the aggregation of probe, which caused by electrostatic attraction. In this way, the turn-on detection of protamine is achieved, and the detection limit is as low as 30 ng mL −1 . When heparin appears, the probes will be redisperse in solution, which causes a decrease in fluorescence intensity. Besides, this method also shows good selectivity and sensitivity, and the linear range of heparin is from 0.08 to 8 μg mL −1 with detection limit of 37 ng mL −1 . After hydrolyzing heparin by heparinase, the probes rebind with protamine and the fluorescence enhance. The fluorescence enhancement was linearly related to the concentration of heparinase in the range of 0.02–2.0 μg mL −1 and detection limit as low as 143.7 ng mL −1 . In addition, the results exhibited that the recovery percentage of heparinase in bovine samples reached to 96–101%.
研究领域:
生物化学
·
分析化学
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 10
·1-10
·
IF 5.705
Huang Yan-Qin,Yang Li-Na,Wang Yong-Sheng,Xue Jin-Hua,Chen Si-Han
The authors report on a one-pot approach for synthesizing highly fluorescent protamine-stabilized gold nanoclusters. These are shown to be a viable nanoprobe for selective and sensitive fluorometric determination of lead(II) via quenching of fluorescence via Pb(II)-Au(I) interaction. Under optimized conditions, fluorescence measured at excitation/emission peaks of 300/599 nm drops in the 80 nM–15 μM lead(II) concentration range. The detection limit is 24 nM, and relative standard deviations (for n = 11) at concentrations of 0.10, 4.0 and 15 μM are 1.6, 2.5 and 1.9%, respectively. The relative recoveries of added lead(II) in the water samples ranged from 97.9 ± 2.29% to 101.2 ± 1.83%.
Lead(II) ions are found to be able to selectively and sensitively quench the fluorescence of the protamine-gold nanoclusters (PRT-AuNCs). Thereby, an inexpensive, selective and sensitive lead(II) assay was established.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemosensors
·
2026
·Vol. 14
·期 2
·51
·
IF 4.4
Nana Ma,Xueling Dong,Ruinan Li,Chuang Du,Yawen Wang,Jiaxin Bai,Run Ran,Xulin Liu,Dianshuo Zhang,Haikui Zou
Heparin (Hep) and its clinical antidote protamine (PRO) play essential yet antagonistic roles in anticoagulant therapy, necessitating reliable analytical tools to monitor their levels and interactions. Herein, we report that coptisine chloride (COP), a natural isoquinoline alkaloid, acts as an aggregation-induced emission (AIE) sensor enabling dual-responsive fluorescence modulation toward Hep and PRO. Owing to its rigid polycyclic and intrinsically twisted molecular framework, COP displays typical AIE behavior. In a DMSO/PBS mixture (PBS fraction = 99%,v/v), COP forms strongly emissive aggregates with Hep through electrostatically driven complexation, allowing sensitive Hep detection with a limit of detection (LOD) of 0.70 μg/mL. Subsequent competitive binding of PRO to Hep disrupts the COP–Hep aggregates, giving rise to fluorescence quenching and reversible PRO sensing (LOD: 0.49 μg/mL). Theoretical calculations together with multiple characterization techniques reveal an aggregation–disaggregation mechanism governing the dual fluorescence modulation. Moreover, COP achieves accurate Hep quantification in spiked diluted human serum, affording satisfactory linearity and recoveries (LOD = 0.71 μg/mL; recoveries 98.3–101.6%). These results demonstrate that COP, a structurally simple natural AIE luminogen, serves as a sustainable, biocompatible, and accessible tool for reversible Hep and PRO analysis in complex media.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）