SPECTROSCOPY LETTERS
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2013
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IF 0.667
Pingping Li,Jing Song,Yizhong Shen,Youqiu He
Aqueous glutathione-capped cadmium/tellurium quantum dots with a diameter of about 3 nm were synthesized. The fluorescence was quenched in the presence of protoporphyrin IX disodium salt, with the excitation wavelength at 320 nm. Under the optimal conditions, the quenched fluorescence intensity was linear in the range of 0.096–16 µg · mL−1 with a concentration of protoporphyrin IX disodium salt, and the detection limit (3σ) was 2.8 × 10−2 µg · mL−1. The proposed method has been applied to the determination of protoporphyrin in serum samples with satisfactory results. The interaction mechanism was investigated.
研究领域:
分析化学
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生物医学
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DOI
高影响因子
JOURNAL OF RAMAN SPECTROSCOPY
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2020
·Vol. 51
·期 12
·2447-2457
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IF 2
Kainan Zhang,Xinjie Cai,Yaru Guo,Chuanzi Liu,Tao Jiang,Yining Wang
Changes in mineral content (mainly phosphate) are important for detecting primary dental caries. However, so far, there has not been a device that can provide detailed information on the changes in organic and inorganic content of dental caries during the caries development and sensitively found early dental caries. DIAGNOdent has been widely used as a laser device for detecting dental caries, but it does not provide information about dental inorganics. Our experiment synthesised hydroxyapatite-aromatic amino acids (HA-AAAs), protoporphyrin IX (HA-PPIX), and tetracycline hydrochloride (HA-TCN) complexes as a simple biological mineralization model related to teeth in vitro and achieved single-factor control to avoid impurity interference so that the test results are accurate. For the first time, Raman and DIAGNOdent were used to detect HA-AAAs, HA-PPIX, and HA-TCN and determine the fluorescence characteristics of each organic substance. Our results show that Raman results are consistent with DIAGNOdent readings when detecting complexes and that Raman relative intensity (RRI) calculated by Raman spectroscopy can directly reflect the phosphate content in the sample. This implies that Raman is possible to replace DIAGNOdent as a novel and more accurate caries detector after conversion of the spectrum results.
研究领域:
牙科学
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生物矿化
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光学检测技术
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2018
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IF 8.097
Mengmeng Xu,Hui Zhou,Yanan Liu,Jing Sun,Wenjie Xie,Ping Zhao,Jie Liu
Alzheimer’s disease (AD) has become one of the most serious societal problems globally, with no effective treatments. Parenchymal accumulation of amyloid beta (Aβ) plaques and the formation of neurofibrillary tangles are the hallmarks of AD. Their possible interactions and synergistic effects in AD have been gradually elucidated. The failure of many clinical trials suggests that it is difficult to treat AD with a focus on a single target. Instead, multiple targets may be an important direction for AD drug research. In this study, we used protoporphyrin IX (PX)-modified oxidized mesoporous carbon nanospheres (OMCN) (PX@OMCN@PEG(OP)@RVGs) as a novel AD multifunctional nanodrug having multiple targets. The nanodrug efficiently inhibits tau phosphorylation. In addition, the use of PX with focused ultrasound triggered the production of reactive oxygen species that significantly inhibited Aβ aggregation. Both approaches notably increased the cognitive level of APP/PS1 transgenic (Tg) mice and ultimately achieved dual-target inhibition of AD. Furthermore, the safe and effective delivery of PX across the blood–brain barrier (BBB) due to modification of the RVG peptide was demonstrated in vivo and in vitro. The favorable photothermal effect of the nanoparticles improved the BBB permeability of PX@OP@RVGs under near-infrared irradiation. The results demonstrated that the novel PX@OP@RVG multifunctional nanomedicine has a dual-target treatment capability for AD and can traverse the BBB, indicating the potential for the effective treatment of AD.
研究领域:
神经科学
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药物递送
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生物医学工程
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DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2026
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IF 8.8
Jiyang Chu,Liangmin Wei,Beibei Chu,Xingguang Chen,Feiran Xu,Baocai Xu
A model system was established for zinc protoporphyrin IX (ZnPP) formation. • The system is based on pepsin-digested myoglobin as the sole heme substrate. • Acidification and proteolysis synergistically release heme from myoglobin. • Intracellular enzymes convert released heme into ZnPP and protoporphyrin IX (PPIX). • A reducing microenvironment protects porphyrins and sustains ZnPP formation. Zinc protoporphyrin IX (ZnPP) is a natural red pigment formed in fermented meat products, in which lactic acid bacteria are thought to play a key role; however, the mechanism remains unclear. This study aimed to investigate ZnPP formation by Leuconostoc mesenteroides subsp. mesenteroides (LM). A model system based on pepsin-digested myoglobin was established, and the conversion process was characterized by fluorescence spectroscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, and biochemical analyses. After optimizing the key parameters, the LM strain increased ZnPP production by 89.08% compared with the uninoculated control under the optimal conditions of 60 min pepsin digestion and 200 μM Zn 2+ . During fermentation, the strain acidified the system from pH 6.5 to 4.7–5.0 and secreted extracellular proteases, which together disrupted the myoglobin aggregates and promoted heme release. The mobilized heme was then converted into ZnPP and protoporphyrin IX (PPIX) through intracellular enzymatic activity, accompanied by an increase in non-heme iron and a decrease in free Zn 2+ . Additionally, the LM strain enhanced the total antioxidant capacity and reduced protein carbonyl content, indicating the formation of a reducing microenvironment that may protect porphyrin intermediates and enzyme activities. Collectively, these findings provide physiological and biochemical evidence for the key steps involved in ZnPP formation by LM and support a multi-step cooperative model for the conversion of myoglobin heme into ZnPP. Download: Download high-res image (248KB) Download: Download full-size image
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高影响因子
JOURNAL OF FLUORESCENCE
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2020
·Vol. 30
·期 6
·1463-1468
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IF 2.093
Xue Gao,Zhiying Ma,Xiuying Liu,Lijun Tang,Jianrong Li
Graphene quantum dots (GQDs), which have high photostability, anti-photobleaching and scintillation, good biocompatibility and low toxicity, are important member of the fluorescent material family, and have attracted extensive research interest. In this paper, a fluorescence resonance energy transfer (FRET) biosensor based on protoporphyrin IX (PpIX) and GQDs was developed for melamine detection. PpIX was bound to the surface of GQDs to produce self-assembled nanosensors, and a FRET process occurred between GQDs and PpIX. However, due to the combination of melamine and PpIX, the FRET process was shut down in the presence of melamine. The FRET system could quickly and accurately detect melamine with a detection range of 1.0 × 10 −8 to 2.0 × 10 −6 mol/L based on the fluorescence intensity ratio of PpIX and GQDs, and the detection limit was 3.6 × 10 −9 mol/L. This method obtained satisfactory results when it was employed to the determination of melamine in milk samples.
研究领域:
分析化学
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纳米技术
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食品安全
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DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
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2024
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IF 5.8
Zhixia Gong,Tiaozhen Yu,Zhi Cai,Ying Yi,Jingli Zhang,Weiquan Cai,Yihua Yin,Guanghua He,Yaqi Xiao,Peiyu Xu,Huiyi Wu,Sihui Lin
Photoactivated pesticides with high activity, environmental friendliness, and no drug resistance represent the direction of pesticide development. However, poor photostability and lack of targeting ability greatly restrict their practical application. Herein, a novel amphiphilic photoactivated polymeric propesticide was synthesized by conjugation of mPEG with the photo/enzyme-labile o-nitrobenzyl ester group followed by grafting protoporphyrin IX (PpIX), which could self-assemble into photo/esterase responsive polymer micelles in aqueous solutions for a controlled release of PpIX. The fluorescence quenching caused by the aggregation of PpIX into hydrophobic core could enhance its photostability. UV/esterase stimulation could trigger the release of PpIX and restore its photodynamic activity. Compared to a single stimulus, dual stimulation could trigger more PpIX release and resulting in higher photodynamic activity and antibacterial effect. In addition, light exposure also showed little influence on the photodynamic activity and antibacterial effect of the system due to enhanced photostability. Compared with PpIX, the nanosystem exhibited higher wettability and retention ability on plant leaves and could resist rainwater washout. Weed control experiment also demonstrated that the system’s control effect on barnyard grass. Therefore, this study provides new ideas for promoting the application of photoactivated pesticides in the field of plant protection.
研究领域:
植物保护
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农药科学
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2025
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IF 3.9
Lu Zhengnan,Wang Yi,Zhang Peng,Sheng Yang,Sun Yixin,Bradley Mark,Zhang Rong
Locally induced photodynamic therapy (PDT) with internally generated light could offer opportunities for the efficient treatment of deep tissue tumors and bacteria hidden in places where external light cannot reach. To achieve this, luminol acrylamide was synthesized and used to prepare nanospheres that were loaded with a polyurethane photosensitizer and the enzyme horseradish peroxidase. With H 2 O 2 the nanoparticles were self-illuminating and showed good antibacterial abilities and also anticancer effects via a PDT mechanism indicating efficient self-luminous functionality. The self-luminating nanospheres could potentially be used in sterilization and for cancer depression.
研究领域:
纳米医学
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光动力疗法
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癌症研究
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抗菌治疗
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生物医学工程
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高影响因子
ANALYTICAL SCIENCES
·
2016
·Vol. 32
·期 8
·887-892
·
IF 1.174
Kang Bei Hua,Li Na,Liu Shi Gang,Li Nian Bing,Luo Hong Qun
Herein is reported a simple and label-free fluorescent detection method for hemin based on using protoporphyrin IX (PPIX) as a fluorescent signal reporter. PPIX emits weak fluorescence in an aqueous solution. When PPIX binds to G-quadruplexes, the fluorescence intensity of PPIX is greatly increased. While in the presence of target hemin, hemin competes with PPIX toward G-quadruplexes because its affinity to G-quadruplexes is higher than that of PPIX. With the formation of the hemin-G-quadruplex complex, PPIX is released to the solution from the G-quadruplex accompanied by quenching of the fluorescence of the system. This fluorescence change of the system can be used to monitor hemin with a low detection limit of 36 nM. In addition, the possible binding sites for PPIX binding to the G-quadruplex are discussed based on competition between hemin and PPIX. What is more, this method might pave the way for applying G-quadruplexes and PPIX to more sensing systems.
研究领域:
分析化学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
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