Antibiotics-Basel
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2023
·Vol. 12
·期 4
·686
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IF 4.8
Ting Qin,Kai Chen,Bingwen Xi,Liangkun Pan,Jun Xie,Liushen Lu,Kai Liu
Aeromonas hydrophilais a Gram-negative bacterium that widely exists in various aquatic environments and causes septicemia in fish and humans. Resveratrol, a natural polyterpenoid product, has potential chemo-preventive and antibacterial properties. In this study, we investigated the effect of resveratrol onA. hydrophilabiofilm formation and motility. The results demonstrated that resveratrol, at sub-MIC levels, can significantly inhibit the biofilm formation ofA. hydrophila, and the biofilm was decreased with increasing concentrations. The motility assay showed that resveratrol could diminish the swimming and swarming motility ofA. hydrophila. Transcriptome analyses (RNA-seq) showed thatA. hydrophilatreated with 50 and 100 μg/mL resveratrol, respectively, presented 230 and 308 differentially expressed genes (DEGs), including 90 or 130 upregulated genes and 130 or 178 downregulated genes. Among them, genes related to flagellar, type IV pilus and chemotaxis were significantly repressed. In addition, mRNA of virulence factors OmpA, extracellular proteases, lipases and T6SS were dramatically suppressed. Further analysis revealed that the major DEGs involved in flagellar assembly and bacterial chemotaxis pathways could be regulated by cyclic-di-guanosine monophosphate (c-di-GMP)- and LysR-Type transcriptional regulator (LTTR)-dependent quorum sensing (QS) systems. Overall, our results indicate that resveratrol can inhibitA. hydrophilabiofilm formation by disturbing motility and QS systems, and can be used as a promising candidate drug against motile Aeromonad septicemia.Keywords:resveratrol;Aeromonas hydrophila;biofilm formation;motility;RNA-seq;quorum sensing
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FOOD CHEMISTRY
·
2014
·
IF 3.259
Limin Xiang,Lingyun Xiao,Yihai Wang,Haifeng Li,Zebo Huang,Xiangjiu He
Moderate consumption of red wine reduces the risk of heart disease and extends lifespan, which these healthy benefits are often attributed to its high antioxidant content. The relative contributions of wine polyphenols in healthy benefits were studied in this study. Among all wine polyphenols, caffeic acid was the richest one, while gallic acid showed the highest free radical scavenging activity. There was no significant difference between the prime red wine and the red wine adding 10-fold resveratrol on neuroprotective effects on SH-SY5Y cell line. The contribution percentage of resveratrol to the antioxidant activity of red wine was less than other tested polyphenols. It suggested that resveratrol may be negligible with respect to healthy benefits of red wine.
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Foods
·
2024
·Vol. 13
·期 23
·3810
·
IF 4.7
Song Yu,Lianpeng Zou,Jiawei Zhao,Yiping Zhu
Fumonisin B1 is a common food contaminant that has been found to adversely affect the reproductive system, especially Sertoli cells. However, the potential mitigation of FB1-induced cytotoxicity in Sertoli cells has not been fully elaborated. Resveratrol is a natural substance with anti-inflammatory, antioxidant, and anti-tumor properties. Herein, the protective effects of resveratrol against FB1-induced cytotoxicity in Sertoli cells were examined in this work. The mouse Sertoli cell line (TM4) was used as a research model. These results indicated that FB1 (40 μM and 80 μM) significantly reduces cell viability, disrupts the cell barrier, and induces an inflammatory response in TM4 cells. To our surprise, resveratrol (15 μM) showed an ability to reverse adverse effects induced by FB1 (40 μM). Furthermore, resveratrol could alleviate the FB1-induced apoptosis, decrease ROS level, and promote the antioxidant enzymes (CAT and SOD2) expression in FB1-treated TM4 cells. The addition of resveratrol could mitigate FB1-induced promoted phosphorylation of JNK and upregulation of c-jun expression. Interestingly, resveratrol was also able to mitigate the cytotoxicity of FB2 (40 μM), FB3 (40 μM), and an FB1-FB2-FB3 (40 μM-40 μM-40 μM) combination group on TM4 cells. In summary, this research displayed that resveratrol may alleviate fumonisin B1-induced cytotoxicity in Sertoli cells via inhibiting oxidative stress-mediated JNK/c-jun signaling pathway-induced apoptosis. This study provides new insights into the prevention and treatment of FB1-induced testicular toxicity and highlights the potential application value of resveratrol.
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JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
·
2014
·Vol. 140
·期 12
·2065-2075
·
IF 3.009
Li Peichun,Yang Sen,Dou Mengmeng,Chen Youran,Zhang Jie,Zhao Xiaoyan
Purpose The aim of this study was to investigate whether resveratrol (Res) combined with artemisinin (ART) possess synergistic effect on different cancer cells. Materials and methods The viability of HepG2 and HeLa cells treated with ART and Res was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Combination index (CI) analysis and isobologram were used to assess the synergistic effect of ART and Res in different ratios. Wound-healing assay was used to investigate the migration rate. AO staining and fluorescent microscopy measurements were performed to detect the cell apoptosis. Reactive oxygen species (ROS) was measured with 2′7′-dichlorofluorescein diacetate (DCFH-DA). Results MTT assay indicated that ART and Res inhibited the growth of HeLa and HepG2 cells in a dose-dependent manner. The combination of ART and Res exhibited the strongest anticancer effect at the ratio of 1:2 (ART to Res). The combination of the two drugs also markedly reduced the ability of cell migration. Apoptosis analysis showed that combination of ART and Res significantly increased the apoptosis and necrosis rather than use singly. Additionally, ROS levels were elevated by combining ART with Res. Conclusions Taken together, the present study suggested that ART and Res possessed the synergistic anti-tumor effect. ART in combination with Res could be an effective therapeutic strategy for cancer.
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JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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2016
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IF 1.685
Jinxin Che,Junling Shi,Zhenhong Gao,Yan Zhang
An enzymatic reaction system was developed and optimized for bioconversion of resveratrol from glucose. Liquid enzyme extracts were prepared from Alternaria sp. MG1, an endophytic fungus from grape, and used directly or after immobilization with sodium alginate. When the enzyme solution was used, efficient production of resveratrol was found within 120 min in a manner that was pH-, reaction time-, enzyme amount-, substrate type-, and substrate concentration-dependent. After the optimization experiments using the response surface methodology, the highest value of resveratrol production (224.40 µg/l) was found under the conditions of pH 6.84, 0.35 g/l glucose, 0.02 mg/l coenzyme A, and 0.02 mg/l ATP. Immobilized enzyme extracts could keep high production of resveratrol during recycling use for two to five times. The developed system indicated a potential approach to resveratrol biosynthesis independent of plants and fungal cell growth, and provided a possible way to produce resveratrol within 2 h, the shortest period needed for biosynthesis of resveratrol so far.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Zhenyu Wang,Yulin Lei,Xiwei Peng,Huifang Zhang,Huanqing Niu,Yong Chen,Chenjie Zhu,Dong Liu,Hanjie Ying
Resveratrol is an important natural product with a variety of biological activities. Plants and fruits are the primary dietary sources of resveratrol. To produce resveratrol more efficiently at a nutraceutical grade, a grape suspension cell culture was developed as an alternative resource for resveratrol. Subsequently, surface-adhesion-based immobilization of the grape cells was exploited to establish a continuous (repeated-batch) production process. The maximum resveratrol production by immobilized cells in the first batch culture reached 413 mg/L but dropped apparently in the second batch. A preimmobilization approach was further developed to enhance the grape cell adhesion on the carrier, which greatly improved the stability of continuous production. The immobilized cells were used continuously for 56 days throughout seven repeated batches with an average production of 163 mg/L, 2.2-fold higher than that of the suspension culture. This study demonstrated that surface adhesion of plant cells could be an effective method for the long-term continuous production of plant metabolites.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Shengtao Bo,Sui Kiat Chang,Ting Zhou,Hong Zhu,Yueming Jiang,Bao Yang
Prenylated stilbenoids are good candidates of nutraceuticals presented in food resources. The levels of natural prenylated stilbenoids are usually low. Biotransformation is a promising synthesis strategy to produce novel bioactive compounds. However, information regarding biosynthesis of prenylated stilbenoids is rare. In this work, prenyltransferase and geranyl diphosphate biosynthesis pathway were overexpressed in E. coli . Multiple prenyltransferase genes were tested and Ambp1 was found to be effective on resveratrol geranylation. The products were identified by mass spectrometry and nuclear magnetic resonance spectroscopy as 4- C -geranyl resveratrol ( 1 ) and 3- O -geranyl resveratrol ( 2, novel chemical). By optimization of culture conditions, a yield of 36.9% was achieved for the conversion to geranylated resveratrol from resveratrol. These two compounds demonstrated good antioxidant activities with IC 50 values of 28.09 μM for 4- C -geranyl resveratrol and 403.88 μM for 3- O -geranyl resveratrol. The results were helpful for developing novel technique to produce prenylated phenolics.
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高影响因子
Materials
·
2022
·Vol. 15
·期 16
·5552
·
IF 3.748
Weiwei Zhu,Jiajie Long,Meiwu Shi
(1) Background: Supercritical CO2fluid (SCF-CO2)-assisted impregnation presents advantages on loading active drugs to polymer substrates, since it enables the realization of a drug-loaded polymer without any solvent residue. Besides, CO2gas and drugs can be recycled and utilized again. Resveratrol-loaded diacetate fiber by SCF-CO2-assisted impregnation was done to give diacetate fiber biological activity function for enhancing its added value. (2) Methods: The effect of SCF-CO2temperature, pressure and treatment time on loading ability (LA) of resveratrol onto diacetate fiber was explored by ultraviolet visible spectrophotometer. The variation of structure and property of diacetate fiber was analyzed by characterization instruments. (3) Results: LA had been increasing with SCF-CO2treatment time, temperature and pressure when SCF-CO2was above 70 °C, 12 MPa. The inhibiting rate of resveratrol to free radicals was affected positively by SCF-CO2. After resveratrol was impregnated by SCF-CO2it appeared some small white granular substances on the surface of diacetate fiber. It had a good interaction between resveratrol and molecular chain of diacetate fiber. (4) Conclusions: Resveratrol was well-loaded onto the diacetate fiber by SCF-CO2assisted impregnation.Keywords:diacetate fiber;supercritical CO2fluid;resveratrol;loading ability
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JOURNAL OF DENTISTRY
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2021
·
IF 4.379
Rui Guo,Wenan Peng,Hongye Yang,Chenmin Yao,Jian Yu,Cui Huang
Objectives This paper aimed to evaluate the influence of resveratrol-doped adhesive on the durability and antibiofilm capability of dentin bonding . Methods Experimental adhesives were prepared by incorporating resveratrol into a universal adhesive at concentrations of 0 (control), 0.1, 1, and 10 mg/mL. The microtensile bond strength, fracture modes, and adhesive–dentin interface nanoleakage were assessed after 24 h of water storage, 10,000 times of thermocycling or 1-month of collagenase ageing. Relevant antibiofilm capability on Streptococcus mutans ( S. mutans ), in situ zymography , degree of conversion, and cytotoxicity of resveratrol-doped adhesives were also determined. Results Irrespective of thermocycled or collagenase ageing, the resveratrol-doped adhesive (1 mg/mL) maintained the bond strength and reduced the nanoleakage expression. Meanwhile, the inhibitory ability on endogenous protease activity and S. mutans biofilm formation with acceptable biocompatibility were obtained. Conclusions This study suggested that the resveratrol-doped adhesive achieved effective improvement on dentin bond durability and secondary caries management. Clinical significance The application of the resveratrol-doped adhesive indicates promising benefits to increase the lifetime of composite restorations.
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Food & Function
·
2021
·Vol. 12
·期 22
·11449-11459
·
IF 5.396
Erdong Yuan,Shiying Nie,Chunli Qi,Bo Chang,Jiaoyan Ren
Resveratrol has a variety of physiological activities, but its bioavailability in the body is low. In this study, the interaction between the peptide SH, prepared from Scomberomorus niphonius, and resveratrol was judged by fluorescence spectroscopy. Then, SHa1 was obtained by the purification of SH, and its effect on the characteristics of resveratrol was studied. SHa1 interacted with resveratrol at 37 °C for 30 min to obtain the complex SHa1-R, which then showed an obviously stronger inhibition on B16 cells than resveratrol using the MTT assay after in vitro gastrointestinal digestion. The solubility and digestive stability of SHa1-R were higher than that of free resveratrol. The intestinal absorption rate of SHa1-R was also increased compared with resveratrol according to the non-inverted rat intestinal sac model. The structure of SHa1 was analyzed by UPLC, auto amino acid analysis, and UPLC-MS/MS. The molecular weight of SHa1 was mainly concentrated under 1000 Da, and it was rich in glutamic acid, aspartic acid, lysine, and leucine. Eighteen possible peptides were identified from SHa1. The results suggested that the peptide SHa-1 may help to increase the bioavailability of resveratrol by increasing the solubility, digestive stability and intestinal absorption of resveratrol, thereby promoting its inhibitory effect on B16 cells.
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ACS Applied Nano Materials
·
2023
·
IF 5.9
Shulin Du,Wenjing Wang,Shuwei Liu,Wenzhe Xu,Ze Wang,Li Rong,Wenjie Feng,Qilin Liu,Yi Liu,Hao Zhang
Natural polyphenol compounds have exhibited multiple therapeutic effects in numerous reports. However, there is still a strong desire to combine polyphenols with other functional components to explore novel therapeutic systems. Herein, we demonstrate the construction of core–shell nanostructures with a variety of water-dispersed nanoparticles (NPs), including polymers, noble metals, metal alloys, semiconductors, preassembled NPs as the core, and resveratrol-formaldehyde resin as the shell. The as-prepared core–shell nanostructures inherit the bioactivities of resveratrol and the functionalities of the encapsulated NPs. More importantly, the thicknesses of the resin shell can be further regulated based on treatment requirements. In vitro studies demonstrate that the resveratrol-formaldehyde resin-encapsulated NPs exhibit a promising antitumor property due to their selective and responsive cytotoxicity toward cancer cells. Benefiting from the universality of this encapsulation strategy, as well as the diversity of polyphenols and functional NPs, it is believed that this work will promote the development of therapeutic techniques based on polyphenols.
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CRYSTENGCOMM
·
2017
·Vol. 19
·期 41
·6154-6163
·
IF 3.474
Hongyan He,Qi Zhang,Jian-Rong Wang,Xuefeng Mei
It was reported that the alkaloid component piperine (Pip) can drastically improve the bioavailability of the compound resveratrol (RSV) when co-administered. Therefore, cocrystallization experiments of RSV and Pip were attempted, and four cocrystals are successfully obtained from solution evaporation experiments. Their single crystals are acquired and the structural determination reveals that all the cocrystals contain solvent molecules within the crystals. Confirmed by VT-PXRD, the cocrystals transformed into the amorphous state after desolvation. Considering the toxicity of the solvent molecule, only RSV–Pip co-1 was utilized for further dissolution and pharmacokinetic experiments. The dissolution results demonstrate that RSV–Pip co-1 shows lower dissolution and apparent solubility than the RSV constituent under different buffers in the presence of different surfactants. The reduced dissolution and apparent solubility have a strong diminishing impact on the absorption of RSV, thus leading to unfavorable bioavailability, even though the coformer Pip possesses bioenhancer properties.
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