Bioengineering & Translational Medicine
·
2022
·Vol. 7
·期 3
·e10395
·
IF 10.684
Ruiyi Zhou,Lihong Zhu,Zhaohao Zeng,Rixin Luo,Jiawei Zhang,Rui Guo,Lei Zhang,Qunying Zhang,Wei Bi
Alzheimer's disease (AD) is an aging-related neurodegenerative disease. The main pathological features of AD are β-amyloid protein (Aβ) deposition and tau protein hyperphosphorylation. Currently, there are no effective drugs for the etiological treatment of AD. Rifampicin (RIF) is a semi-synthetic broad-spectrum antibiotic with anti-β-amyloid deposition, anti-inflammatory, anti-apoptosis, and neuroprotective effects, but its application in AD treatment has been limited for its strong hydrophobicity, high toxicity, short half-life, low bioavailability, and blood–brain barrier hindrance. We designed a novel brain-targeted and MRI-characteristic nanomedicine via loading rabies virus protein 29 (RVG29), rifampicin, and Gd on poly ( l -lactide) nanoparticles (RIF@PLA-PEG-Gd/Mal-RVG29). The cytotoxicity assay demonstrated that RIF@PLA-PEG-Gd/Mal-RVG29 had favorable biocompatibility and security. Fluorescence imaging in vivo showed that PLA-PEG-Gd/Mal-RVG29 could deliver rifampicin into the brain by enhancing cellular uptake and brain targeting performance, leading to improvement of the bioavailability of rifampicin. In in vivo study, RIF@PLA-PEG-Gd/Mal-RVG29 improved the spatial learning and memory capability of APP/PS1 mice in the Morris water maze, as compared to rifampicin. Immunofluorescence, TEM, immunoblotting, and H&E staining revealed that RIF@PLA-PEG-Gd/Mal-RVG29 reduced Aβ deposition in hippocampal and cortex of APP/PS1 mice, improved the damage of synaptic ultrastructure, increased the expression level of PSD95 and SYP, as well as reduced the necrosis of neurons. These findings suggest that RIF@PLA-PEG-Gd/Mal-RVG29 may be an effective strategy for the treatment of AD.
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Materials Today Advances
·
2023
·
IF 10
Xiaodan Tang,Yichao Zhao,Hongmei Yu,Shuanping Cui,Hunter Temple,Eric Amador,Yun Gao,Ming-li Chen,Shaoyan Wang,Zhizhi Hu,Wei Chen
Carbon dots have many new and interesting phenomena, and the concentration dependent luminescence wavelength is an intriguing one. Herein, nitrogen-sulfur co-doped carbon dots (NSCDs) diluted to different concentrations, the high concentration of G-NSCDs (0.2 mg mL −1 ) with green fluorescence and the low-concentration of B-NSCDs (0.01 mg mL −1 ) with blue fluorescence were obtained. Further, it was found that the detection behaviors are different for different concentrations. The “turn-off” fluorescent sensor of G-NSCDs can specifically recognize rifampicin in a linear range of 0.2–20 μM with a detection limit of 56.6 nM employing the mutual effect of photo-induced electron transfer and dynamic quenching. Meanwhile, a “turn-off” B-NSCDs fluorescence sensor was constructed to effectively identify morin based on the internal filtration effect and static quenching, achieving a suitable linearity of 0.2–30 μM and a detection limit of 51.2 nM. Due to the strong chelation of morin and Al 3+ , a “turn-on” fluorescent probe for sequential detection Al 3+ was fabricated based on B-NSCDs-morin system, obtaining a good linearity of 0.1–2.0 μM with a satisfactory detection limit of 45.8 nM. These interesting behaviors indicate that NSCDs are expected to become novel sensing materials for the monitoring of rifampicin, morin and Al 3+ in organisms, which is beneficial for our health.
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Food Science & Nutrition
·
2020
·Vol. 8
·期 7
·3590-3601
·
IF 1.797
Jun Yang,Ting Peng,Jiyong Huang,Guohua Zhang,Jiaheng Xia,Maomao Ma,Danwen Deng,Deming Gong,Zheling Zeng
Drug-induced liver injury (DILI) is one of the common adverse effects of drug therapy, which is closely associated with oxidative stress, apoptosis, and inflammation response. Medium-chain fatty acids (MCFA) were reported to relieve inflammation and attenuate oxidative stress. However, little has been known about the hepatoprotective effects of MCFA in DILI. In the present study, acetaminophen (AP) and rifampicin (RFP) were used to establish DILI models in LO2 cells, and the cytoprotective effects of MCFA on hepatocellular injury were investigated. Results showed that the optimal condition for the DILI model was treatment with 10 mM AP or 600 µM RFP for 24 hr. LCFA treatment markedly reduced the cell viability and increased the activities of alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase. Meanwhile, LCFA treatment aggravated cell apoptosis, mitochondrial dysfunction, and oxidative stress. The mRNA and protein expression levels of inflammatory cytokines (IL-1β and TNF-α) were significantly elevated by LCFA. In contrast, MCFA treatment did not significantly affect cell viability, apoptosis, oxidative, stress and inflammation, and it did not produce the detrimental effects on DILI models. Therefore, we proposed that MCFA may be more safe and suitable than LCFA as nutrition support or the selection of daily dietary oil and fat for the patients with DILI.
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JOURNAL OF SOLID STATE CHEMISTRY
·
2018
·
IF 2.179
Jian Su,Xiaofeng Xiang,Rui Lv,Hui Li,Xin Fu,Boyi Yang,Wen Gu,Xin Liu
In this work, a simple, rapid and high-selectivity method for detection of rifampicin based on upconversion luminescence core-shell structure NaYF 4 :Yb, Tm@SiO 2 composites(UCCS) is developed via hydrothermal method , encapsulated with silica shells and characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), Transmission electron microscopy (TEM), photoluminescence (PL) and lifetime measurement. Systematical luminescence studies showed that NaYF 4 :Yb, Tm@SiO 2 represents a high quenching efficiency on detecting rifampicin through inner filter effects (IFE). Meanwhile, the fluorescence intensity of NaYF 4 :Yb, Tm@SiO 2 decreased with the gradually increasing concentration of rifampicin and the low detection limit is 8.5 μΜ. The sensing system was successfully applied in detection of rifampicin in human urine samples for the first time with high recyclability and the fluorescence quenching mechanism of UCCS/rifampicin mixture were also investigated deeply.
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ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Jiahang Liu,Yingying Chu,Xiaoyang Li,Mengying Qian,Weiming Zhang
The co-catalytic effect of hydroquinone/quinone electron shuttle has received great attention in the area of Fenton-like reaction. However, the crucial roles of phenolic or hydroquinone moieties in the chemical structures of organic pollutants have always been overlooked. In this study, we discovered that the introduction of rifampicin (RIF), a representative of rifamycin antibiotics, could trigger complete degradation of atrazine (ATZ) in the Fe(III)/H 2 O 2 system. By exploring the influences of initial Fe(III), H 2 O 2 , RIF concentrations and pH values on ATZ degradation efficiency, key components affecting metal ion valence transitions in the system were identified. Quenching experiments, probe tests and EPR analysis verified that •OH served as the primary active species, while HO 2 •/O 2 •− was also generated. Through monitoring Fe(II) concentration variations and employing mass spectrometric analyses, the oxidation pathway of RIF was elucidated: When mixed with Fe(III), the naphthohydroquinone moiety of RIF immediately acted as a direct electron donor to reduce Fe(III) to Fe(II), while itself being oxidized to RIF-Q. Upon H 2 O 2 addition, it rapidly triggered the classical Fenton reaction with Fe(II) to generate •OH, enabling ATZ degradation. Simultaneously, RIF-Q underwent further oxidative breakdown, with some degradation products retaining the capacity to continuously donate electrons to Fe(III), thereby promoting its reduction. This study provides new insights for the co-catalytic effect of pollutant-derived electron shuttles on the transition of metal ion valences.
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BULLETIN OF THE KOREAN CHEMICAL SOCIETY
·
2018
·Vol. 39
·期 5
·657-664
·
IF 0.522
Nai-Di Tan,Jian-Hang Yin,Yaqing Yuan,Lei Meng,Na Xu
Herein, a novel bright-green and highly fluorescent polyethyleneimine capped copper nanoclusters (CuNCs@PEI) has been hydrothermally synthesized, which is employed as a fluorescence probe to determine rifampicin. Under optimum synthetic conditions, the morphology, surface chemistry and optical features of resulting CuNCs@PEI are well characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, UV–Vis absorption spectra, transient and steady-state fluorescence. In comparison to previous reports, the new species with a larger diameter of approximately 3 nm exhibit higher fluorescence quantum yield (QY) up to nearly 10.7%, and demonstrate tolerability to extreme pH, high ionic strengths as well as long-term UV irradiation. These excellent optical properties ascribe to unique grain size and surface chemical features of products, which are further discussed in this work. In addition, we employed as-prepared CuNCs@PEI to detect rifampicin based on fluorescence quenching phenomenon. The fluorescence intensity of probe is linearly proportional to rifampicin over the concentration rage from 0 to 20 μM, where the limit of detection (LOD) is 50 nM. Besides, this probe can be also used to assay rifampicin in serum samples with satisfactory recoveries.
研究领域:
分析化学
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纳米生物技术
·
药物分析
·
分子诊断
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转化医学
·
药代动力学
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Yi Zhang,Qingbo Deng,Chang Tang,Minglu Zhang,Zilong Huang,Zhifeng Cai
Development of excellent sensors to determine trace concentrations of rifampicin is of intense importance for medicine analysis and human health. Herein, a facile and green fluorescent probe was established for the determination of rifampicin by using folic acid protected copper nanoclusters (FA-Cu NCs). Many characterization methods were applied for the analysis of the as-prepared FA-Cu NCs including UV–visible absorption spectra, fluorescence spectra, Fourier-transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), fluorescence lifetime and X-ray photoelectron spectroscopy (XPS). The TEM image suggested that the as-prepared FA-Cu NCs were highly dispersed. The as-synthesized FA-Cu NCs emerged blue fluorescence under UV light and demonstrated maximum emission wavelength at 446 nm under the maximum excitation wavelength of 358 nm. After the addition of rifampicin, the FL intensities of FA-Cu NCs were uncommonly quenched. The related experimental data intimated that the quenching mechanisms were assumed to the inner filter effect (IFE) and static quenching. The as-proposed probe platform displayed an obvious linear relationship with rifampicin concentrations varying from 0.5 to 100 µM, and the corresponding detection limit (LOD) was 0.073 µM (S/N = 3). Finally, the as-established detection platform was successfully employed to analyze trace concentrations of rifampicin in real samples.
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ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Wanling Cai,Xiulan Weng,Zuliang Chen
Antibiotics in water and soil are persistent, bioaccumulative and toxic to aquatic organisms and human health. To address it, as one of the new technologies , green synthesized magnetic Fe 3 O 4 nanoparticles by Excoecaria cochinchinensis extract used to remove rifampicin (RIF) was investigated in this study. Results showed the adsorption efficiency of RIF reached 98.4% and the maximum adsorption capacity is 84.8 mg/g when 20 mL of RIF at a concentration of 20 M was adsorbed by 10 mg Fe 3 O 4 at a temperature of 303 K. The morphology of the green Fe 3 O 4 characterized by SEM demonstrated the dimensions ranging from 20 to 30 nm. The N 2 adsorption/desorption isotherms revealed that the surface area of Fe 3 O 4 was 111.8 m 2 /g. In addition, adsorption studies indicated that the kinetics fitted the pseudo second-order and isothermal adsorption conformed to the Langmuir isotherm. Furthermore, due to their magnetic properties, the Fe 3 O 4 nanoparticles were easily separated and reused and the mechanism for removing RIF occurred through adsorption rather than chemical redox reaction. Finally, the reusability of Fe 3 O 4 for adsorption of RIF showed that the removal efficiency decreased to 61.5% after five cycles.
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ACS Applied Bio Materials
·
2024
·
IF 4.6
Zhaoxin Xia,Yulong Liao,Ge Gao,Shiyong Zhang
The poor accumulation of antibiotics in the cytoplasm leads to the poor eradication of intracellular bacteria. Herein, a polyelectrolyte complex (PECs@Rif) allowing direct cytosolic delivery of rifampicin (Rif) was developed for the treatment of intracellular infections by complexation of poly(α-lipoic acid) (pLA) and oligosaccharide (COS) in water and loading Rif. Due to the thiol-mediated cellular uptake, PECs@Rif delivered 3.9 times higher Rif into the cytoplasm than that of the free Rif during 8 h of incubation. After entering cells, PECs@Rif released Rif by dissociating pLA into dihydrolipoic acid (DHLA) in the presence of intracellular thioredoxin reductase (TrxR). Notably, DHLA could reduce endogenous Fe(III) to Fe(II) and provide a catalyst for the Fenton reaction to produce a large amount of reactive oxygen species (ROS), which would assist Rif in eradicating intracellular bacteria. In vitro assay showed that PECs@Rif reduced almost 2.8 orders of magnitude of intracellular bacteria, much higher than 0.7 orders of magnitude of free Rif. The bacteremia-bearing mouse models showed that PECs@Rif reduced bacterial levels in the liver, spleen, and kidney by 2.2, 3.7, and 2.3 orders of magnitude, respectively, much higher than free Rif in corresponding tissues. The direct cytosolic delivery in a thiol-mediated manner and enhanced oxidative stress proposed a feasible strategy for treating intracellular bacteria infection.
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MOLECULES
·
2023
·Vol. 28
·期 14
·5496
·
IF 4.6
Ting Li,Chaoyue Yang,Houkang Cao,Siyi Mo,Bo Li,Zhipeng Huang,Ruobing Zhang,Jianzhao Wu,Kefeng Zhang,Ya Gao
Bergenin (BER), a natural component of polyphenols, has a variety of pharmacological activities, especially in improving drug metabolism, reducing cholestasis, anti-oxidative stress and inhibiting inflammatory responses. The aim of this study was to investigate the effects of BER on liver injury induced by isonicotinic acid hydrazide (INH) and rifampicin (RIF) in mice. The mice model of liver injury was established with INH (100 mg/kg)+RIF (100 mg/kg), and then different doses of BER were used to intervene. The pathological morphology and biochemical indicators of mice were detected. Meanwhile, RNA sequencing was performed to screen the differentially expressed genes and signaling pathways. Finally, critical differentially expressed genes were verified by qRT-PCR and Western blot. RNA sequencing results showed that 707 genes were significantly changed in the INH+RIF group compared with the Control group, and 496 genes were significantly changed after the BER intervention. These differentially expressed genes were mainly enriched in the drug metabolism, bile acid metabolism, Nrf2 pathway and TLR4 pathway. The validation results of qRT-PCR and Western blot were consistent with the RNA sequencing. Therefore, BER alleviated INH+RIF-induced liver injury in mice. The mechanism of BER improving INH+RIF-induced liver injury was related to regulating drug metabolism enzymes, bile acid metabolism, Nrf2 pathway and TLR4 pathway.Keywords:bergenin;isonicotinic acid hydrazide;rifampicin;liver injury;RNA sequencing
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Journal of Cleaner Production
·
2019
·
IF 6.395
Ze Lin,Xiulan Weng,Gary Owens,Zuliang Chen
Most environmental contaminants exist as a complex mixture of both metallic and organic pollutants and it is thus often a major challenge to remove both components simultaneously. In this paper, due to its low cost and environmentally friendliness, iron nanoparticles (Fe NPs) were successfully prepared using a one-step green synthesis for the simultaneous in-situ remediation of mixed contaminants. Specifically Fe NPs were used for the simultaneous removal of Pb(II) and rifampicin. The removal efficiency for Pb(II) and rifampicin were 100% and 91.6%, respectively. To better understand the mechanism of mixed contaminant removal by Fe NPs various characterization methods and the major conditions impacting removal efficiency were examined. It was concluded that (1) polyphenols and caffeine, both ubiquitous in green tea extracts, acted as both reducing and capping agents during the preparation of Fe NPs, reducing the agglomeration of Fe NPs and increasing their stability and reactivity; (2) the removal mechanism of contaminants involved Pb(II) being adsorbed onto the surface of Fe NPs due to the innate strong adsorption of metal ions onto iron oxides , while rifampicin and Pb(II) both interacted positively with the functional groups on the surface of Fe NPs. Overall, this study demonstrated that green synthesis Fe NPs had potential for use as inexpensive and efficient adsorbent for the simultaneous removal of both Pb(II) and rifampicin from wastewater.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Zhi-Wei Pan,Ting-Ting Du,Qiuyu Wu,Meng-Ting Wang,Hao Pan,Tianyue Zhang,Qiang Liu,Wen-Jing Yi
Rifampicin (RIF), a critical anti-tuberculosis drug and broad-spectrum antibiotic, poses significant risks to food safety and public health when misused. To ensure efficient monitoring, we developed a ‘two-pronged’ sensing strategy using yellowish-green fluorescent carbon dots (CDs) for both laboratory analysis and on-site detection. The CDs exhibit excitation-independent photoluminescence and robust photostability. In the laboratory mode, RIF selectively quenches the CDs' fluorescence, achieving a detection limit (LOD) of 0.67 μM (linear range: 3–25 μM). For on-site application, a paper-based sensor was constructed by depositing CDs onto filter paper. Quantitative analysis of RIF on test strips was realized via a custom-developed WeChat mini-program, ‘RIF Tester’, which converts colorimetric changes into concentrations under ambient light, achieving high recoveries (88.7% ~ 110.0%) across a 0–90 μM range. This study marks the first development of a smartphone-integrated, instrument-free paper platform for rapid RIF monitoring, offering a powerful tool for resource-limited settings.
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