FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Yujing Lu,Yicheng Guo,Lina Wang,Yoshimasa Nakamura,Hang Qi
Polyphenols extracted from Ascophyllum nodosum (PEAn) exhibit significant bioactivities but are limited by poor photothermal stability and low membrane permeability, which hinder their applications. This study explores the use of royal jelly exosomes (RExo) as a novel food-derived nanocarrier to enhance the stability and targeted delivery of PEAn. RExo (105.01 ± 7.24 nm) demonstrated a spherical morphology and expressed key exosomal markers (CD63, CD81, TSG101), enriched in metabolism-related proteins. The yield of RExo was optimized to a 12.72-fold increase at pH 6.0 compared to pH 2.0. Ultrasound-assisted loading achieved 85.73 ± 1.73 % encapsulation efficiency, as confirmed by spectroscopic analysis. Encapsulation significantly enhanced the photothermal stability and gastrointestinal tolerance of PEAn. In B16F10 cells, RExo improved PEAn uptake, inhibited tyrosinase activity, reduced melanin synthesis, and suppressed cell migration. Mechanistically, the anti-melanogenic effect of PEAn-RExo may be partially dependent on autophagy induction. This study provides a promising natural strategy for the development of functional foods and personalized nutrient delivery systems.
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高影响因子
MOLECULES
·
2026
·Vol. 31
·期 5
·
IF 5.1
Jing Sun,Yu Zhang,Hao Zhang,Rui Chen,Lin Zhang,Fengmao Liu,Xiaofeng Xue
Royal jelly, a nutrient-rich bee product characterized by high water content and active components, is particularly susceptible to quality deterioration during storage. While temperature effects have been extensively documented, the specific role of light exposure in quality degradation remains largely unexplored. despite its relevance during production, handling, transportation, and display. This study systematically investigated the volatile organic compounds (VOCs) of royal jelly under different storage conditions using gas chromatography-ion mobility spectrometry (GC-IMS) combined with fingerprint analysis. Results from dual-column validation demonstrated that even short-term light exposure at 25 °C induced pronounced alterations in VOC profiles, triggering the accumulation of off-flavor aldehydes (e.g., hexanal, nonanal) and ketones, along with 2-furfural generated via Maillard reaction. Concurrently, characteristic fresh-aroma esters and alcohols were significantly depleted. Multivariate statistical analysis confirmed light exposure as the predominant factor driving quality deterioration, with temperature variation under dark conditions producing comparatively minor effects within the same short timeframe. This work provides the first systematic evidence establishing insights into early volatile changes in royal jelly and identifies key VOC markers that offer valuable insights for optimizing storage strategies and developing rapid quality monitoring protocols.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Shixuan Su,Shanshan Li,Jing Hu,Ting Yu,Lingchen Tao,Fuliang Hu,Yunlei Xianyu
Royal jelly as a functional food has great potential to promote human health. As a unique ingredient in royal jelly, 10-hydroxy-2-decenoic acid (10-HDA) is a vital criterion to evaluate the quality of royal jelly. Current approaches for 10-HDA detection mainly rely on high-performance liquid chromatographic (HPLC), but it requires large-scale equipment and skilled technicians that are costly and time-consuming. Herein, we report a colorimetric sensing strategy for 10-HDA detection based on Ag(I) and tetramethylbenzidine (TMB), a widely used chromogenic reagent. Ag(I) can oxidize TMB to generate oxidized TMB (oxTMB) showing a light-blue color. Due to the chelation with Ag (I), the introduction of 10-HDA can prevent the generation of oxTMB that results in a color fading and a decrease in absorbance. This colorimetric sensing strategy enables highly sensitive detection of 10-HDA with a linear range from 1 μM to 10 μM and a limit of detection of 0.13 μM. More importantly, this 10-HDA assay allows for a visual readout either by a smartphone or the naked eye with low cost, convenience, and rapid response that has been successfully applied for evaluating the quality of royal jelly in actual samples. The sensing strategy developed in this work provides a new colorimetric approach that holds great promise as a point-of-care platform for the food industry.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Shuai Zhuang,Huihui Ming,Wenjie Yu,Liping Luo
Royal jelly (RJ) is a highly perishable food during storage. However, the quality of RJ is hard to evaluate due to its complex and variable compositions. Nanoliter electrospray ionization mass spectrometry (nano-ESI-MS) is an emerging direct mass spectrometry method suitable for rapid analysis of complex food components and food quality. In this study, we investigated the freshness and metabolite changes of RJ stored at 4 °C and 25 °C using nano-ESI-MS and verified the results with UPLC-Q/TOF-MS. The nano-ESI-MS identified 20 compounds and quantified 9 metabolites with high stability and reproducibility. Multivariate analysis of the nano-ESI-MS data distinguished RJ of different freshness and suggested 45–60 and 7 days as the freshness threshold of RJ stored at 4 °C and 25 °C, respectively. The multivariate analysis of UPLC-Q/TOF-MS data output similar results to that of nano-ESI-MS. The relative abundance changes of metabolites identified by UPLC-Q/TOF-MS were approximately consistent with the quantification results of nano-ESI-MS, indicating the reliability of nano-ESI-MS in monitoring RJ freshness. This study proved the potential of nano-ESI-MS as a rapid method for monitoring RJ freshness and thus promoted the quality control of RJ during production.
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PLoS One
·
2019
·Vol. 14
·期 2
·e0212335
·
IF 2.776
Yifan Zhang,Yong Chen,Yiting Cai,Zongyan Cui,Jinjie Zhang,Xiaohou Wang,Lirong Shen
Honey adulteration is becoming increasingly alarming incidents in food safety. Monitoring and detecting adulteration face greater challenges. Honey contains the major royal jelly proteins (MRJP) secreted by bee workers. To detect honey adulteration fast and accurately, a rapid gold sandwich immunochromatographic strip (GSIS) was developed based on two specific polyclonal antibodies (PoAbs) against the MRJP1, the most abundant protein of all MRJPs. We determined the best of pH value (pH 8.6) and PoAb SP-1 amount (5 μg/mL) in conjunction with colloidal. The cut-off value (sensitivity) of GSIS in detecting MRJP1 is 2.0 μg/mL in solution. Validation analysis with RJ, milk vetch honey, acacia honey and honey adulteration containing rice syrup and corn syrup with different ratios demonstrated that the GSIS could show consistent Test line (T line) when the test samples contain more than 30% pure honey or MRJP1 0.4 mg/g. The validation results by isotope ratio mass spectrometry on the same pure and all adulteration milk vetch honey samples showed the same information of GSIS test. The qualitative assay GSIS provided a valuable new way for honey authenticity and laid the foundation for the future application of GSIS with monoclonal antibodies in honey authentication.
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npj Science of Food
·
2026
·
IF 9.7
Wang Xingyang,Zhou Enning,Liu Dongyuan,Zheng Daojun,Qi Huasha,Chen Dafu,Wu Liming,Li Qiangqiang
Current research on 3D-printed foods for dysphagia has largely prioritized printability and structural fidelity, with limited attention to nutritional and biological functionality. We fabricated Pickering emulsions (PEs) loaded with Alpinia oxyphylla essential oil (AoEO) using royal jelly protein (RJP) as a natural stabilizer. Rheological analysis revealed gel-like behavior (tanδ < 1) and shear-thinning properties. At 40–50 mg/mL protein, storage modulus ( G ’) and apparent viscosity increased with protein concentration and oil volume fraction, promoting a stable three-dimensional network. The PE with 50 mg/mL protein and 70% oil phase showed superior extrudability and self-support, meeting IDDSI Level 5 (Minced & Moist) requirements. Bioactivity assays showed the MIC and MBC values of this PE were 10 and 30 mg/mL, while its IC 50 values for DPPH and ABTS radical scavenging were 4.236 and 62.751 mg/mL, respectively. Microstructural and FTIR analysis indicated that RJP formed a dense barrier at the oil-water interface, effectively encapsulating AoEO, reducing volatile loss, and enabling controlled release during intestinal digestion. These findings confirm that the emulsion possesses antibacterial and antioxidant effects, favorable 3D-printing properties, and sustained-release capacity, offering practical benefits for dysphagia patients.
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Journal of Functional Foods
·
2024
·
IF 3.8
Lu Xiu,Rongguang Ge,Siyu Lu,Linwei Li,Wenqi Huang,Guojun Du,Zengli Zhang,Jie Zhang,Zhongxiao Wan,Jie Chang
Diabetes, a ruthless chronic metabolic disorder, is increasingly considered to jeopardize global well-being over the decades. Royal jelly (RJ) is a complex natural product secreted by worker bees containing a unique fatty acid known as 10-hydroxy-2-decenoic acid (10-HDA). This study aimed to verify the protective effect on cognitive function of RJ and 10-HDA in diabetic mice and explore the underlying mechanism. Briefly, our animal experiment results suggested that 10-HDA had a significant hypoglycemic effect and improved cognitive performance. Through western blotting, RJ and 10-HDA intervention notably increased Atg7, Atg16L1, Beclin-1, Atg5, Atg12, Atg3, and LC3-II expression and decreased p62 expression. Meanwhile, decreased mTOR expression and increased ULK1 expression were also observed in the 10-HDA intervention group. In conclusion, 10-HDA can regulate mTOR/ULK1 pathway to activate autophagy to alleviate hippocampal damage, thereby improving cognitive function in mice with diabetes, which may provide a new option for real-world cognitive impairment management.
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高影响因子
PHYTOMEDICINE
·
2025
·
IF 8.3
Xue-Ting Xu,Jun-Kai Fan,Xi Chen,Si-Yi Que,Run-Xin Zhang,Yu-Yan Xie,Tian-Ci Zhou,Kunmei Ji,Zhen-Fu Zhao,Jia-Jie Chen
Background Mast cells (MCs) mediate high-affinity IgE Fc receptor (FcεRI)-mediated allergic reactions. Royal jelly acid (RJA), the major lipid constituent of royal jelly, has anti-inflammatory, anti-tumor, antibacterial, and immunomodulatory properties. Purpose This study aimed to investigate how RJA affects IgE-mediated MC activation and to elucidate mechanisms underlying anti-allergic effects. Methods Mouse bone marrow-derived mast cells (BMMCs) and rat basophilic leukemia cells-2H3 (RBLs) were stimulated with IgE-antigen (Ag). β-hexosaminidase (β-hex) and histamine (HA) release, inflammatory cytokines expression, and cellular morphology changes were examined in stimulated cells. Transcriptome changes associated with RJA-treated activated-MCs were analyzed. Network pharmacology, gene expression, and western blot analyses were employed to explore the relationship between H3 lactylation and anti-allergic effects of RJA on MC. In vivo studies were conducted in IgE-mediated passive cutaneous anaphylaxis (PCA), ovalbumin (OVA)-induced active systemic anaphylaxis (ASA), and HA-induced passive systemic anaphylaxis (PSA) mouse models. Results RJA inhibited IgE-dependent MC activation as evidenced by reduced β-hex and HA release. RJA inhibited MAPK and NF-κB signaling, down-regulated RELA and NFκB1 transcripts, and decreased NF-κB reporter activity. RJA inhibited cellular H3 lactylation and H3K9 lactylation (H3K9la), leading to reduced histone lactyllysine enrichment in RELA or NFκB1 promoter loci. RJA alleviated allergic symptoms in PCA and ASA mice by intraperitoneal injection or oral administration, but did not affect HA-induced hypothermia. Conclusion RJA inhibits IgE-dependent MC activation by reducing cellular H3K9la and suppressing NF-κB signaling via reduced histone lactyllysine enrichment in RELA or NFκB1 promoter sites. RJA may support MC inhibition in the prevention and treatment of allergic diseases.
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高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2024
·Vol. 68
·期 19
·2400098
·
IF 4.5
Xinqi Huang,Lu Xiu,Yumei An,Yuan Gong,Sunao Li,Xueshi Chen,Chao Liu,Jianghuiwen Lu,Haiyan Shan,Jie Chang,Mingyang Zhang
The objective of this study is to elucidate how Royal jelly (RJ) and 10-hydroxy-2-decanoic acid (10-HDA) prevents diabetic skin dysfunction by modulating the pyroptosis pathway. Type 2 diabetes models are induced by fat diet consumption and low dose of streptozotocin (STZ) in C57BL/6J mice and treated with RJ (100 mg kg –1 day –1 ) and 10-HDA, the major lipid component of royal jelly (100 mg kg −1 day −1 ) for 28 weeks. The results show that serum concentrations of glucose and triglyceride are significantly lower in the RJ group or 10-HDA than diabetes mellitus (DM) group. Compared to the control group, pyroptosis proteins, GSDMD, ASC, Caspase-1, and IL-1β are increased in the skin of the diabetic model, accompanied by the activation of the Wnt/β-catenin signal pathway. Further evaluations by RJ exhibit superior improvement of skin damage, repress activation of the Wnt/β-catenin pathway, and attenuate keratinocyte pyroptosis, but 10-HDA cannot completely suppress the activation of Wnt/β-catenin pathway and pyroptosis, which shows a relatively weak protective effect on skin damage which shows that RJ is a better effect on skin injury after DM.
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Food Analytical Methods
·
2020
·Vol. 14
·期 3
·616-630
·
IF 2.667
Qi Dandan,Ma Chengying,Wang Wenwen,Zhang Licui,Li Jianke
Royal jelly produced by nurse bees from tea tree (tea royal jelly) is a special bee product in China and it is popular with consumers owing to its multiple healthcare functions. However, the volatile compound profile of tea royal jelly has not been investigated. To obtain a comprehensive understanding of volatile compound profile of tea royal jelly, a method based on headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME/GC-MS) and chemometric analysis was applied. Moreover, two royal jelly samples produced from pagoda tree were also investigated to reveal the difference in volatile compounds between royal jelly produced from tea tree and other plants. A total of 66 volatile compounds were identified in tea royal jelly, including 16 alkanes, 14 aldehydes, 9 alcohols, 9 ketones, 8 esters and lactones, 5 phenols, 2 organic acids, and 3 other compounds, and most of them were discovered in royal jelly for the first time. Notably, α -ionone, an important aroma compound in tea, was first identified in tea royal jelly. Moreover, the result of chemometric analysis indicates a significant difference on volatile compound profile between royal jelly produced from tea and pagoda trees. To sum up, 35 differential compounds were identified and they could well distinguish royal jelly samples. Our data offers the first characterization of volatile compound profile of tea royal jelly and significantly expands the knowledge of volatile compounds in royal jelly. It is potentially useful as a new approach to distinguish royal jelly produced from different botanical origins.
Graphical abstract
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高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2014
·Vol. 37
·期 14
·1826-1833
·
IF 2.594
Dan Liu,Tianbin Zhang,Yechun Cheng,Qiong Jia
A polymer monolith microextraction method coupled with high-performance liquid chromatography was developed for the determination of adenosine triphosphate, adenosine diphosphate, and adenosine monophosphate. The monolithic column was synthesized inside fused-silica capillaries using thermal initiation free-radical polymerization with glycidyl methacrylate as the monomer, ethylene dimethacrylate as the cross-linker, cyclohexanol, and 1-dodecanol as the porogen. N -Methylolacrylamide, an important hydrophilic monomer, was incorporated into the polymerization mixture to enhance the hydrophilicity of the poly(glycidyl methacrylate- co -ethylene dimethacrylate) column. The obtained poly(glycidyl methacrylate- co - N -methylolacrylamide- co -ethylene dimethacrylate) monolith was characterized by scanning electron microscopy, Fourier-transform infrared spectra, and X-ray photoelectron spectroscopy. Optimum conditions for the preconcentration and separation of the target adenosines were also investigated. Under the optimum conditions, we obtained acceptable linearities, low limits of detection, and good relative standard deviations. The developed polymer monolith microextraction with high-performance liquid chromatography method exhibited a good performance with recovery values in the range of 76.9−104.7% when applied to the determination of the adenosines in five royal jelly samples.
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Food Analytical Methods
·
2018
·Vol. 12
·期 3
·705-711
·
IF 2.245
Tu Xijuan,Wu Siyuan,Liu Weiyi,Gao Zhaosheng,Huang Shaokang,Chen Wenbin
An analytical method based on sugaring-out assisted liquid-liquid extraction (SULLE) and HPLC-fluorescence detection has been developed for the determination of bisphenol A (BPA) and bisphenol B (BPB) in royal jelly. Experimental parameters of SULLE including the extraction solvent, amount of phase separation agent, initial concentration of acetonitrile (ACN), and sample size were investigated. The optimal SULLE procedure involves 0.3 g sample extracted by 2 mL ACN-water mixture (50%, v / v ), followed by phase separation with the addition of 0.4 g glucose. The obtained extract was injected into the reversed-phase HPLC system without clean-up step. The chromatography separation of BPA and BPB from royal jelly matrix was achieved in the mobile phase composed of 40% ACN and 60% water with isocratic elution at 1.0 mL/min. Limits of quantification for BPA and BPB were 40 μg/kg and 45 μg/kg, respectively, and limits of detection for BPA and BPB were 16 μg/kg and 18 μg/kg, respectively. Average recoveries of BPA and BPB at three spiked levels were in the range of 88.32~93.59% and 95.14~97.48%, respectively. Precision expressed as relative standard deviations (RSDs) in the interday and intraday analysis were all lower than 5%. The developed method was applied to the analysis of ten commercial and eight raw royal jelly samples. Results indicated that none of BPA and BPB were detected in these 18 samples. The proposed method is simple, fast, and sensitive, and could be used for the monitoring of BPA and BPB contamination in royal jelly.
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高影响因子
危险品化学品经营许可证（带存储）