JOURNAL OF FOOD SCIENCE
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2021
·Vol. 86
·期 5
·1714-1725
·
IF 3.167
Hai-Mei Li,Ting-Ting Xu,Qing-Xia Peng,Yong-Sheng Chen,Hua Zhou,Yu-Yun Lu,Ri-An Yan
Rutin (3′,4′,5,7-tetrahydroxy-flavone-3-rutinoside) was enzymatically acylated with benzoic acid and its esters (methyl benzoate and vinyl benzoate) using Thermomyces lanuginosus lipase (Lipozyme TLIM). The acylation reaction was optimized by varying the reaction medium, reaction temperature, acyl donor, substrate molar ratio, and reaction time. The highest conversion yield (76%) was obtained in tert -amyl alcohol (60 °C, 72 hr) using vinyl benzoate (molar ratio of 1:10) as acyl donor. The acylation occurred at the 2′′′-OH and 4′′′-OH of the rhamnose unit and the 2′′-OH position of the glucose moieties. Three novel rutin acylated derivatives (compounds 1 – 3 ) were purified and characterized by HR-MS and 1D and 2D NMR spectroscopy. We found that acylation significantly improved lipophilicity, capacity to inhibit lipid peroxidation, anticancer capacity and substantially maintained the antioxidant activity of rutin. This research provides important insights in the acylation of flavonoids with different glycosyl moieties. Practical Application In this study, three novel rutin derivatives were successfully synthesized and the highest conversion yield (76%) was obtained by reacting the rutin and vinyl benzoate at molar ratio of 1:10 in tert -amyl alcohol for 72 hr at 60 °C. Introducing a benzoic acid substituent into rutin molecule significantly improved their lipophilicity and inhibition of lipid peroxidation in lipophilic system. Furthermore, this study demonstrated that acylation significantly improved anticancer capacity and substantially maintained the antioxidant activity.
研究领域:
食品科学
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生物化学
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药物化学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 5
·1-8
·
IF 5.705
Wang Zonghua,Yu Gege,Xia Jianfei,Zhang Feifei,Liu Qingyun
The authors demonstrate the exploitation of reduced graphene oxide (RGO) as a template for immobilizing zeolitic imidazolate framework-8 (ZIF-8) crystals loaded with the electrochemical probe Methylene Blue (MB). The framework was deposited on the surface of RGO in a one-pot process. Transmission electron microscopy, scanning electron microscopy and X-ray diffraction were employed to characterize the nanocomposite. The electrochemical behavior of rutin at a glassy carbon electrode (GCE) modified with the nanocomposite was investigated by cyclic voltammetry and differential pulse voltammetry. The modified GCE displays high electrocatalytic activity toward rutin oxidation at a relatively low working potential (0.4 V vs. Ag/AgCl). Under the optimal conditions, the sensor has an amperometric response that is linear in the 0.1 to 100 μM rutin concentration range, with a 20 nM detection limit (at an S/N ratio of 3). The method was successfully applied to the determination of rutin in tablets and urine samples.
The zeolitic imidazolate framework ZIF-8 was loaded with Methylene Blue and deposited on the surface of reduced graphene oxide. A glassy carbon electrode was modified with the nanocomposite and then used for the determination of rutin with a 20 nM detection limit and a linear range from 0.1 to 100 μM.
研究领域:
材料科学
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分析化学
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药物分析
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DOI
PubMed
高影响因子
Fermentation-Basel
·
2024
·Vol. 10
·期 2
·87
·
IF 3.3
Wenjiao Xue,Chen Liu,Yao Liu,Hao Ding,Chao An,Shizhe Zhang,Saijian Ma,Qiwen Zhang
To assess the probiotic potential of strain 68-1 with rutin conversion capabilities, isolated from Chinese traditional Jiangshui, a complete genomic analysis and in vitro tests were conducted. The Oxford Nanopore Technologies (ONT, Oxford, UK)–Illumina (San Diego, CA, USA) hybrid sequencing platform was used for whole genome sequencing and the results showed that strain 68-1 had a chromosome sequence of 3,482,151 bp, with 46.53% GC content and five plasmids with a sequence length ranging from 2009 bp to 48,711 bp. Strain 68-1 was identified asLactiplantibacillus pentosusbased on the whole genome sequence. A total of 133 genes encoding for carbohydrate-active enzymes (CAZymes) were identified and genes that may be involved in rutin conversion were found in theL. pentosus68-1 genome.L. pentosus68-1 showed excellent tolerance to gastrointestinal juice and adhesion properties, and corresponding genes were identified. In addition,L. pentosus68-1 exhibited strong antibacterial and antifungal activity, where organic acids may play a crucial role in its antagonistic ability. Moreover, the gene cluster for plantaricin_EF production was detected. No high virulence factor was found in theL. pentosus68-1 genome and no hemolytic effect was observed. In addition,L. pentosus68-1 showed resistance to ampicillin, gentamycin, and kanamycin, and the genomic analysis indicated that horizontal ARG transfer should not be possible. The results show thatL. pentosus68-1 could be developed as a novel probiotic candidate to improve rutin bioavailability in the food and feed industry.
研究领域:
微生物学
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食品科学
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基因组学
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
·
IF 5.4
Jiahui Tong,Yifan Zhao,Xiao Ning,Hongyi Peng,Jingyu Yan,Danlei Qin,Feng Tian,Xiuping Wu,Chunfang Wang,Bing Li
Wound healing is often impeded by bacterial infections, excessive reactive oxygen species (ROS), and inflammatory responses, challenges that traditional dressings inadequately address. Rutin, a natural flavonoid recognized for its antioxidant and anti-inflammatory effects, is limited in biomedical applications because of its low solubility in water. This research presents a novel approach using nitrogen and zinc-doped rutin-derived carbon dots (ZR-CDs), which enhances both antioxidant activity and bioavailability by leveraging the nanoscale properties and surface modifiability of carbon dots. The ZR‑CDs are covalently integrated into an oxidized hyaluronic acid (OHA)/Pluronic F‑127 hydrogel through Schiff‑base chemistry, yielding an injectable, shape‑adaptive composite hydrogel (ZCD@OF), aimed at promoting the healing of infected wounds. In vitro, ZR-CDs exhibit excellent biocompatibility, free-radical scavenging, and protection against ROS-induced cellular dysfunction. The ZCD@OF hydrogel exhibited robust antibacterial efficacy while maintaining favorable rheological properties for wound coverage. In vivo on an infected wound model, ZCD@OF significantly accelerates wound closure, promotes angiogenesis and collagen deposition, and suppresses pro-inflammatory cytokines (IL-6, TNF-α). This study highlights the therapeutic potential of ZCD@OF hydrogel in complex wound treatment, illustrating its capacity to synergistically integrate carbon dot with advanced biomaterial strategies for improved therapeutic outcomes.
研究领域:
材料科学
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生物医学工程
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药理学
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DOI
高影响因子
Frontiers in Microbiology
·
2025
·
IF 4.5
Yue Gao,Binbin Huang,Yunyi Qin,Bing Qiao,Mengfei Ren,Liqing Cao,Yan Zhang,Maozhen Han
Background: Obesity and high fasting blood glucose (FBG) resulting from high-fat diets (HFDs) have emerged as significant public health concerns, garnering increasing attention. Recently, gut microbiota has been linked with metabolic diseases such as type 2 diabetes (T2DM), and its mediating role in dietary supplements has been confirmed. Seeking various dietary supplements to lose body weight (BW) and decrease FBG and explaining the underlying mechanism have become the research hotspots in T2DM studies.Methods: In this study, rutin and proanthocyanidins (PA) were selected as dietary supplements (200 mg/kg × day, oral gavage, 6 weeks) in T2DM mice induced with HFD to assess their efficacy in weight loss, FBG reduction, gut microbiota alterations, and the associated underlying mechanisms.Results: Our findings indicate that rutin was more effective than PA in relieving inflammation and fat hypertrophy, although both significantly reduced BW and FBG within 2 weeks after the intervention. Analysis of 16S rRNA amplicons revealed substantial alterations in the gut microbial community composition of mice administered with PA and rutin compared to HFD-fed mice. Importantly, several core microbes, particularly a series of probiotics, such as Akkermansia, Lactococcus, Odoribacter, Faecalibaculum, and Roseburia were identified, which were significantly correlated with the changes in BW and FBG.Conclusion: Overall, our study highlights that rutin and PA can reduce BW, FBG, and inflammation by modulating the gut microbiota composition, providing novel perspectives for managing and treating weight and FBG concerns in obesity and T2DM patients through dietary supplements in clinical treatment.
研究领域:
营养学
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微生物学
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代谢疾病研究
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Rui Liu,Xin Zhang,Ri-jia Liu,Yuan Sun,Bin-qiao Ren
In this study, we created a brand-new g-C 3 N 4 -MWCNTs/Ag/β-CD-MOF electrode that can be used as a platform for the electrochemical sensing of Rutin (RT). The g-C 3 N 4 -MWCNTs/Ag/β-CD-MOF composite was synthesized via calcination treatment and electrodeposition, coupled with hydrothermal reaction. This composite could appreciably enlarge the electrochemically active surface area of the as-prepared sensor to promote electron transfer and result in the enhanced response signals.With the assistance of differential pulse voltammetry (DPV) and cyclic voltammetry (CV), the electrochemical performance of sensor can be greatly enhanced. Under these optimum conditions, the designed electrochemical sensor was able to sense RT at the lower detection limit (LOD) of 0.69 nM (S/N = 3) with a wider range of 5–500 nM and showed high sensitivity of 1.97 μA . nM −1. cm −2 at room temperature. Additionally, the selective investigation on RT was performed with the related interfering molecules, and the g-C 3 N 4 -MWCNTs/Ag/β-CD-MOF/GCE showed decent stability and reproducibility. The real-time application of the modified electrode for RT detection was monitored in both pharmaceutical and serum samples with the satisfactory recovery. Overall, this research contributes to the field of the electroanalytical sensing and offers the potential applications in the drug monitoring.
研究领域:
分析化学
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药物监测
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生物医学工程
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DOI
高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Yan-mei Shi,Li-qin Chao,Lin Mei,Zhi-hong Chen,Xiu-min Li,Ming-san Miao
Herein, a soluble tetramine phthalocyanine indium-decorated reduced graphene oxide (rGO-InTAPc) nanocomposite was prepared via non-covalent functionalization and used to construct a novel electrochemical sensor for sensitive determination of rutin in Tartary buckwheat tea. The rGO-InTAPc as an improved electrode material can significantly increase the electroactive surface area and electron transfer capacity. Moreover, the electrochemical response can be dramatically expanded by the enrichment effect of rutin through the adsorption of π-π stacking. Under optimized conditions, the rGO-InTAPc sensor displayed a linear dependence for rutin concentration ranging from 0.005 to 100 μM with a detection limit of 2.0 nM ( S/N = 3). The proposed sensor with excellent selectivity and reproducibility was quantitatively validated in commercially available Tartary buckwheat tea with rutin level ranging of 9.06–13.87 mg/g, which results were highly consistent with these of HPLC. This strategy of rutin detection will address a long-standing analytical challenge to establishing facile and sensitive methods for quality monitoring in tea drinks.
研究领域:
分析化学
·
食品科学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Kaifeng Yang,Fangming Han,Yafeng Jin,Xiaobo Li
Herein, a stable and ultra-sensitive rutin electrochemical sensor was successfully developed. This sensor based on glassy carbon electrode (GCE) modified with C-GCS@ZIF-F/PL nanocomposite, which was made of thermally carbonized glucose (GCS) doped with flower-like ZIF (ZIF-F) and pencil lead (PL). The electrochemical response of rutin was considerably significant at C-GCS@ZIF-F/PL/GCE, demonstrating favorable conductivity and electrocatalytic properties for detection of rutin. Under optimal conditions, the linear range is 0.1–100 μM, with a low detection limit (LOD) of 0.0054 μM. It also exhibits excellent stability, reproducibility, as well as selectivity over common interfering ions such as Na + , uric acid, quercetin and riboflavin, etc. Meanwhile, the practical utility of developed sensor was evaluated in food samples including honey, orange, and buckwheat tea, achieving satisfactory recovery rates ranging from 98.2% to 101.7%. This paper introduces a novel technique for the detection of rutin in foods.
研究领域:
分析化学
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食品科学
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电化学传感器
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DOI
PubMed
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2020
·
IF 1.415
Li Jun Li,Xiao Qing Liu,Xi Ping Du,Ling Wu,Ze Dong Jiang,Hui Ni,Qing Biao Li,Feng Chen
Isoquercitrin is a flavonoid with important applications in the pharmaceutical and nutraceutical industries. However, a low yield and high production cost hinders the industrial preparation of isoquercitrin. In the present study, isoquercitrin was prepared by biotransformation of rutin using α-L-rhamnosidase from Aspergillus niger JMU-TS528 combined with high-speed countercurrent chromatography (HSCCC) purification. As a result, the optimum transformation pH, temperature, and time were pH 4.0, 60 °C, and 60 min, respectively. The Km and Vmax were 0.36 mM and 0.460 mmol/min, respectively. For isoquercitrin production, the optimal rutin concentration and transformation time were approximately 1000 mg/L and 60 min. The rutin transformation rate reached 96.44%. The isoquercitrin was purified to a purity of 97.83% using one-step purification with HSCCC. The isoquercitrin was identified using UPLC-Q-TOF-MS. The comprehensive results indicated that the biotransformation procedure using the α-L-rhamnosidase from A. niger JMU-TS528 combined with HSCCC was a simple and effective process to prepare isoquercitrin, which might facilitate the production of isoquercitrin for industrial use.
研究领域:
生物化学
·
制药工程
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食品科学
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DOI
PubMed
高影响因子
ACS Omega
·
2024
·
IF 3.7
Yun-qiong Yang,Yuan Zhang,Yang Liu,Feier Lin,Hao Zhang
The construction of tunable white-light-emitting materials has garnered increasing attention in the scientific community. In this study, N-doped carbon dots (N-CDs) were surface-modified with carboxylatopillar[5]arene (CP[5]) using an EDC–NHS coupling reaction to create CCDs. CCDs were then conjugated with rhodamine 6G (R6G) through host–guest interactions to fabricate the CCDs–R6G composites. These composites produced two-color fluorescence emission peaks at 447 and 557 nm when excited by a wavelength of 340 nm. Excitingly, white-light emission (0.28, 0.30) can be readily achieved by varying the R6G concentration. To further explore potential applications, a new detection method for rutin (RT) based on the inner filter effect (IFE) was developed. Experimental results verify the practicality and reliability of the fluorescence sensor based on CCDs–R6G composites for RT detection in real samples.
研究领域:
材料科学
·
分析化学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Abdelhadi El Jaouhari,Luyun Yan,Jinhua Zhu,Dongbao Zhao,Md. Zaved Hossain Khan,Xiuhua Liu
Exceptional sensitivity behavior of molecularly imprinted polymer (MIP) approach towards an electrochemical sensor application encourages combining this approach with other materials to tune their properties such as large surface area, high catalytic effect, electrical, thermal and mechanical stability for the fabrication of sensors with high sensitive performance. In the present work, highly sensitive MIP sensor was developed and successfully applied for rutin recognition. The MIP was decorated on the surface of zeolitic imidazolate framework (ZIF-8) and reduced graphene oxide (rGO) composite modified with glassy carbon electrode (GCE) to fabricate GCE/rGO/ZIF-8/MIP electrode. Spectroscopic and microscopic analyses such as XRD , FT-IR, BET and SEM were used to evaluate the composition and the morphology of the surface of GCE/rGO/ZIF-8/MIP electrode. The electrochemical characterization of electrodes was performed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Also different parameters influencing the sensitivity of GCE/rGO/ZIF-8/MIP, such as the percentage of rGO, template:monomer ratio, number of electropolymerization cycles, accumulation time and pH were optimized. Under optimal conditions, the MIP sensor shows a wide linear range and low limit of detection as well as good reproducibility, stability and selectivity , and used successfully for the determination of rutin in real solutions.
研究领域:
分析化学
·
材料科学
·
药物分析
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产品关联
DOI
PubMed
高影响因子
Current Analytical Chemistry
·
2024
·Vol. 21
·期 2
·124-133
·
IF 1.7
Zhengming Qian,Zhuobin He,Dongyin Lian,Qi Huang,Guoying Tan,Juying Xie
BackgroundFlavonoid is a type of active constituent in herbs and always used as the quality control markers of herbal medicines. Owing to the extensive diversity of flavonoids, numerous reference compounds are necessitated for the analysis of flavonoids, and some are usually very expensive, which engenders challenges in the analysis of flavonoids in herbal medicines. Consequently, the development of a simple, rapid, and reference compounds saving method is important for the determination of flavonoids in herbal medicines.ObjectiveIn order to develop a high-performance liquid chromatography (HPLC) method for the determination of 5 flavonoids (mangiferin, hesperidin, baicalin, buddleoside, and rutin) in five herbal medicines (Anemarrhenae rhizome, Sophorae flos, Citri reticulatae pericarpium, Scutellariae radix, and Chrysanthemi indici flos) with rutin.MethodsFive herbal medicine samples were prepared according to the Chinese Pharmacopoeia which includes ultrasound and reflux methods. The separation of the sample was performed on a PoroShell 120 EC-C18 (4.6 mm×100 mm, 2.7 μm) by gradient elution with 0.1% formic acid and acetonitrile at a flow rate of 1.0 mL/min. The wavelengths were set as follows: Anemarrhenae rhizome (363 nm), Sophorae flos (256 nm), Citri reticulatae pericarpium (236 nm), Scutellariae radix (263 nm), Chrysanthemi indici flos (354 nm).ResultsThe method validation showed that the established HPLC method was accurate and stable for quantitative analysis of flavonoids in five herbal medicines. The comparative analysis revealed that the determination results of the current HPLC method and Chinese Pharmacopoeia method are consistent, exhibiting less than 1% relative error. Remarkably, the developed HPLC method needs one cheapest reference compound (rutin) and costs 8 min for sample HPLC analysis.ConclusionThe developed HPLC method for quantitative analysis of five flavonoids in five herbal medicines is simple, rapid, and reference compound saving, which provides a good alternative method for quality control of flavonoids in herbal medicines.
研究领域:
药学
·
分析化学
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DOI
高影响因子
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