JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 12
·126506
·
IF 3.721
Tingting Hao,Chunfeng Zhang,Han Lin,Wenting Wei,Fan Yang,Yangbo Wu,Lingmei Niu,Weijun Kang,Zhiyong Guo
Herein, a dual-mode aptasensor for subnanomolar detection of lead ions Pb2+ based on electrochemiluminescence (ECL) and fast scan voltammetry (FSV) was developed. It could be one-step fabricated using an all-in-one probe Fe3O4@Au-ssDNA&Ru-NH2 which has multiple functions: Fe3O4 core achieves one-step preparation of the aptasensor; Au shell provides a place for assembly and electrochemical reactions; ssDNA can selectively recognize and capture target Pb2+, and ferrocene labeled on it could provide fast scan voltammetric signal and participate in changes of ECL signals; and Ru-NH2 is a signal marker for ECL detection. In presence of target Pb2+, ssDNA folds to form G-quadruplex, and ferrocene labeled on its end approaches to the Au shell surface resulting in easier electron transfer. So the electrochemical signal is enhanced, and ECL quenching occurs. Electrochemical peak current increased about 1000 times at 100 V s−1 using FSV, making its sensitivity compatible with that of ECL to obtain highly sensitive dual-mode detection. Pb2+ could be detected in the concentration range from 10−10 mol l−1 to 10−6 mol l−1 with a limit of quantitation (LOQ) 10−10 mol l−1. Selectivity, stability, reproducibility, accuracy and practicality are good. This extremely simple method provides a valuable idea for developing biosensors for field detection applications.
研究领域:
分析化学
·
生物传感技术
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Nuo Duan,Wenhui Gong,Shijia Wu,Zhouping Wang
An improved SELEX technique was developed for selecting aptamers against ractopamine (RAC) by immobilizing ssDNA library on the magnetic beads. After sixteen selection rounds, a highly enriched ssDNA pool was sequenced and nine families were grouped according to their homology and secondary structures analysis. One representative aptamer candidate from each family was picked out for binding affinity identification by graphene oxide (GO) adsorption platform. The aptamer RAC-6 was demonstrated as the optimal aptamer with high specificity and dissociation constant (K d ) value of 54.22 ± 8.02 nM. To prove the potential application of aptamer RAC-6 in the quantitative determination of RAC, a fluorescent bioassay with aptamer RAC-6 was developed. The linear range for RAC was from 0.10 ng/mL to 100 ng/mL and the limit of detection was as low as 0.04 ng/mL. Furthermore, the method was validated for the analysis of RAC spiked real samples, and the recoveries were between 82.57% and 104.65%.
研究领域:
分子生物学
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分析化学
·
生物技术
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DOI
PubMed
高影响因子
LUMINESCENCE
·
2023
·Vol. 38
·期 5
·518-526
·
IF 2.9
Yanping He,Jingrui Zhang,Ruifen Tian,Yuying Lu,Ling Pan,Yuzhong Zhang
The abuse of sulfameter (SME) in animal husbandry can cause drug resistance and toxic or allergic reactions in humans. Therefore, it is very important to establish a simple, inexpensive, and efficient method for detecting SME in food. In this work, we propose a single fluorescent aptamer/graphene oxide (GO)-based biosensor to detect SME residues in milk. Aptamers that specifically bind to SME were screened using capture-SELEX and a ssDNA library immobilized on magnetic beads. The 68 active candidate aptamers were chemically synthesized for specificity and affinity characterization. Among the aptamers, the aptamer sulf-1 revealed the highest affinity (K d = 77 ± 15 nM) to SME and was selected to construct a GO-based fluorescent biosensor for real milk sample detection. Under optimal conditions, the single fluorescent aptasensor had a wide linear range (R 2 was 0.997) from 7 to 336 ng/ml and a low detection limit of 3.35 ng/ml that was calculated with a 3SD/slope. The single fluorescent method was also validated using SME-fortified milk samples, showing average recoveries ranging from 99.01% to 104.60% with a relative standard deviation of less than 3.88%. These results demonstrate that this novel aptamer sensor provides an opportunity for sensitive, convenient, and accurate detection of SME residues in milk.
研究领域:
食品科学
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分析化学
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生物技术
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DOI
PubMed
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 27
·3110-3117
·
IF 2.896
Mingyan Song,Chen Lyu,Nuo Duan,Shijia Wu,Imran Mahmood Khan,Zhouping Wang
The abuse of ribavirin, an antiviral drug, in poultry breeding can cause quality degradation and drug resistance. So it is of great importance to establish a simple and effective method for detecting ribavirin in foods. In this work, aptamers that could especially bind to ribavirin with high affinity were obtained by the Capture-SELEX method. After 15 rounds of enrichment, ssDNA library pool was enriched and then analyzed by high-throughput sequencing. The seven most enriched sequences were selected as candidate aptamers for affinity and specificity characterization. Among the candidate aptamers, APT-1 was proved to be the optimal aptamer. The dissociation constant (Kd) values of APT-1 obtained by the two methods of colorimetric and fluorescence were 34.34 ± 6.038 nmol L−1, 61.19 ± 21.48 nmol L−1, respectively. To study the binding mechanism of the selected aptamer, molecular docking was conducted and results indicated that hydrogen bonds were formed at binding sites located at G37, T38, A40, T53 and A54. Furthermore, to confirm the practicability of the selected aptamer, a fluorescence assay was designed, showing the liner range within 1.0–50 ng mL−1 and the low detection limit of 0.67 ng mL−1. Besides, the aptamer was applied for the detection of ribavirin in chicken samples and the recoveries ranged from 87.26% to 105.57%, which showed great application potential in food safety.
研究领域:
分析化学
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食品科学
·
分子生物学
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DOI
PubMed
高影响因子
Cell Reports
·
2026
·Vol. 45
·期 10
·
IF 7.7
Juan Zhang,Mengjiao Lan,Hui Fu,Xucong Teng,Erwei Zhang,Xiran Peng,Chen Li,Bingjie Pan,Hang Yu,Yu Cai,Yan Yan,Yulan Jin,Zongyi Bo,Pumin Zhang,Ying Liu,Dongli Pan,Wei Wu,Jiyong Zhou
Many α-herpesviruses, such as herpes simplex virus type 1 (HSV-1) and pseudorabies virus, possess GC-rich genomes prone to forming G-quadruplex (G4) structures. However, the impact of these G4 structures on viral genome maintenance is unknown. Here, we demonstrate that G4s impede lagging strand DNA synthesis in the viral genome, generating single-stranded DNA (ssDNA) gaps that activate an ATR-mediated replication checkpoint. To complete genome duplication, these viruses co-opt host ssDNA gap prevention pathways, including PARP-mediated backup Okazaki fragment maturation and post-replicative gap-filling mechanisms, to resolve G4-induced ssDNA gaps. Pharmacological G4 stabilization, combined with inhibition of these pathways, synergistically inhibits viral replication. In terminally differentiated neurons, only gap-filling pathways are essential for virus replication, and disrupting them or stabilizing G4s robustly impairs HSV-1 reactivation in trigeminal ganglion explants. Collectively, our findings identify G4-induced ssDNA gaps as key barriers to α-herpesvirus replication and reactivation and highlight host ssDNA gap prevention pathways as promising therapeutic targets.
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DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2018
·
IF 4.155
Tu Jiawei,Gan Ying,Liang Tao,Hu Qiongwen,Wang Qian,Ren Tianling,Sun Qiyong,Wan Hao,Wang Ping
Invisible mercury ion is an extremely poisonous environmental pollutant, therefore, a fast and highly sensitive detection method is of significant importance. In this study, a liquid-gated graphene field-effect transistor (GFET) array biosensor (6 × 6 GFETs on the chip) was fabricated and applied for Hg2+ quantitate detection based on single-stranded DNA (ssDNA) aptamer. The biosensor showed outstanding selectivity to Hg2+ in mixed solutions containing various metal ions. Moreover, the sensing capability of the biosensor was demonstrated by real-time responses and showed a fairly low detection limit of 40 pM, a wide detection ranged from 100 pM to 100 nM and rapid response time below one second. These results suggest that the GFET array biosensor based on ssDNA aptamer offers a simple fabrication procedure and quite fast method for mercury ion contaminant detection and are promising for various analytical applications.
研究领域:
环境科学
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纳米技术
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分析化学
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生物技术
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DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2019
·Vol. 31
·期 9
·095104
·
IF 3.399
Yu Su,Tiantian Xue,Lianpin Wu,Yunliang Hu,Jie Wang,Qianying Xu,Yanyu Chen,Zhenkun Lin
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in the clinic, with the characteristics of occult onset, rapid progression, and high degree of malignancy. Alpha fetoprotein (AFP) is the most important biomarker of HCC, which is widely used in early screening, diagnosis, and prognosis observation. A series of immunoassays have been developed and frequently used in the detection of AFP based on antibodies. Unfortunately, the shortcomings of antibodies, such as thermal unstable and fluctuant activity by batches, lead to the inaccuracy in the detection of AFP. In this study, aptamers instead of antibodies were adopted as the specific recognition element for AFP, aiming to seek an alternative strategy to immunoassays. An AFP-specific ssDNA aptamer was grafted to magnetic nanoparticles (Fe3O4@SiO2) via avidin–biotin interaction, and the resultant aptamer functionalized magnetic nanoparticles (Ap-MNPs) were adequately characterized and tested. The Ap-MNPs in solution exhibited a fast response to the outer magnetic field, and can be completely separated in several minutes. It was found that Ap-MNPs have good specificity to the target AFP, as the recovery of AFP (87.0%) was much higher than the competitive proteins IgG (38.9%), HSA (18.5%), and FIB (11.4%). A convenient and efficient label-free detection method of AFP in serum was developed based on Ap-MNPs in combination with high-performance liquid chromatography. The linearity of this method was over a range of 1–50 μg ml−1 with a correlation coefficient of 0.9999, and the limit of detection was 0.27 μg ml−1. This study indicated that aptamers are an ideal tool for the recognition and detection of biomarkers, and thus will find wide applications in clinical practice.
研究领域:
生物标志物检测
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纳米技术
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癌症研究
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临床诊断
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DOI
PubMed
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 17
·3551
·
IF 4.6
Senhao Hu,Wenjing Wang,Yu Zou,Chunmei Li,Hongyan Zou,Chengzhi Huang,Lei Zhan
The development of low-background, facile, and robust fluorescent nanoprobes for imaging and monitoring of intracellular mRNA changes remains a great challenge. Taking advantage of the high fluorescence quenching efficiency of core-shell gold@polydopamine (Au@PDA) nanocomposites and Ca 2+ -promoting DNA adsorption stability, a simple and universal bioconjugate strategy was designed to a construct fluorescent nanoprobe for highly efficient tumor-related mRNA imaging. The fluorescence of Cy5-labeled DNA was quenched up to 92.38% by the AuNP and PDA via nanometal surface energy transfer (NSET) and photoinduced electron transfer (PET), respectively. TK1 mRNA, a biomarker of tumor growth, initiates hybridization and results in fluorescence recovery, which built the foundation for identifying the expression level changes in living cells. More importantly, three kinds of tumor-related mRNA (TK1 mRNA, GalNAc-T mRNA, and C-myc mRNA) can be detected simultaneously with different fluorophore-modified recognition sequences, which can avoid false positive signals and improve the reliability of cancer diagnostic, holding great promise for cancer diagnosis, prognosis, and therapy.
研究领域:
纳米生物技术
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分子成像
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癌症诊断
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生物分析化学
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基因表达监测
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2018
·Vol. 136
·期 8
·47102
·
IF 1.901
B.-Q. Huang,H. Ding,Z.-L. Xu,Y.-J. Tang
In this work, the biomacromolecule, single-stranded deoxyribonucleic acid (ssDNA) was innovatively incorporated into the polyamide layer to tailor the permeate flux and antifouling performance of the nanofiltration (NF) membranes. With active amines groups, the ssDNA was as the aqueous phase monomers along with piperazine (PIP), and reacted with trimesoyl chloride on polyethersulfone substrate to fabricate thin-film composite (TFC) NF membranes. The NF membrane prepared under optimal ratio of ssDNA/PIP had a pure water permeability of 75.8 L m −2 h −1 (improved 58% compared to PIP NF membrane) and Na 2 SO 4 rejection of 98.0% at 6.0 bar. The rejections for different inorganic salts were the order: Na 2 SO 4 (98.0%) > MgSO 4 (89.2%) > MgCl 2 (72.8%) > NaCl (23.0%). Furthermore, the TFC NF membranes showed good antifouling performance in long-term running with 300 ppm bovine serum albumin and humic acid solution. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135 , 47102.
研究领域:
材料科学
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环境工程
·
生物化学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 24
·19564-19570
·
IF 3.201
Chenggang Shao,Yuxin Liu,Jinxia Qi,Yu Su,Yonghui Chen,Huaguo Xu,Zhenkun Lin,Huaqin Guan
Aptamers have recently been widely employed as recognition elements for the discovery and detection of protein biomarkers. Therefore a comprehensive and in-depth understanding of the interaction between aptamers and their target proteins is especially important. Herein we report a real-time and label-free strategy to detect the interaction between alpha-fetoprotein (AFP), a specific biomarker of hepatocellular carcinoma, and its specific ssDNA aptamer based on dual polarization interferometry (DPI). The ssDNA aptamer was immobilized on the surface of a sensor chip followed by AFP injection, while the changes of mass, thickness and density on the AFP–aptamer interface were monitored and recorded online by DPI. The experimental results showed that there were two different interaction patterns between AFP and the aptamer, which were specific binding and unspecific binding, and the former was found to be the dominant one. AFP binding makes the conformation of the aptamer change, resulting in an increase of mass/thickness and a decrease of density. The concentration of AFP is very important for the AFP–aptamer interaction, as a high AFP concentration will lead to a high probability of the aptamer capturing AFP and therefore a large binding amount and a high mass loading rate. The developed detection method proved effective to study the interaction behavior and mechanism between AFP and its aptamer, and will find wide applications in the detection and understanding of protein–aptamer interaction.
研究领域:
生物化学
·
分子生物学
·
医学诊断
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DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 2
·1-9
·
IF 5.833
Zhang Dongwei,Liu Yang,Ding Jina,Hayat Kashif,Zhan Xuejia,Zhou Pei,Zhang Dan
A short ssDNA (Apt-21) rationally truncated from the parent 100 nt As(III) aptamer was used for colorimetric determination of As(III). Apt-21 serves dual functions, i.e., recognition of trace As(III) and regulation of AuNPs dispersion by surface attachment, while gold nanoparticles (AuNPs) functioned as colorimetric signal reporters. Under the optimal conditions, the ratio of the absorbance at 650 nm to 520 nm (A 650 /A 520 ) of AuNPs changed proportionally with increasing concentration of As(III), which showed a linear relationship within the concentration ranges 1–30 ppb and 30–100 ppb with a detection limit of 0.18 ppb. The feasibility of this assay was demonstrated by determining As(III) in spiked water samples with mean recoveries ranging from 96.5–107.1%. Graphical abstract
Schematic representation of colorimetric detection of As(III) based on the short ssDNA (Apt-21) and gold nanoparticles (AuNPs)
研究领域:
分析化学
·
环境科学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-8
·
IF 5.479
Mao Anqi,Wei Chunying
DNA-templated silver nanoclusters (DNA-Ag NCs) with cytosine (C)-rich sequences (four or more segments of consecutive (2–5) C-bases) were synthesized. They display green and/or orange/red emissions under different excitation wavelengths. There is indication that more consecutive C-bases lead to longer emission wavelengths. The ratio of the red and green emissions of the DNA-Ag NCs depends on whether the NCs were synthesized under acidic or basic conditions. We also prepared the DNA copper/silver nanoclusters (DNA-Cu/Ag NCs) which can be synthesized in shorter time and display higher stability. The DNA-Cu/Ag NCs, under 470 nm photoexcitation, always display green emission, with a peak at 550 nm, irrespective of whether being prepared under acidic or basic conditions. The fluorescence of the Cu/Ag NCs is selectively quenched by Hg(II) ions which can be quantified by this nanoprobe with a detection limit as low as 2.4 nM. The quenching mechanism was studied by Stern-Volmer plots and lifetime studies which revealed that both static and dynamic quenching are operative.
Schematic presentation of fluorescent DNA-Ag nanoclusters (NCs) exhibiting red and green emission under different pH values, and green emissive DNA-Cu/Ag NCs for sensitive and rapid detection of Hg 2+ .
研究领域:
生物化学
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分析化学
·
纳米科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）