JOURNAL OF DAIRY SCIENCE
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2020
·
IF 3.333
Zile Wang,Yuanze Sun,Demei Liang,Yuyang Zeng,Shuang He,Ghulam Mujtaba Mari,Tao Peng,Haiyang Jiang
ABSTRACT Antibiotic residues are major contaminants in milk because of their use in agriculture and animal husbandry. In particular, streptomycin, an aminoglycoside antibiotic, is a potential risk to consumers because of its ototoxicity, anaphylaxis, and growth inhibition. Herein, monoclonal antibodies for streptomycin were conjugated with europium microspheres to serve as detection probes for the development of a chromatographic time-resolved fluoroimmunoassay to detect streptomycin residues in milk. The method had a low detection limit of 0.58 µg/kg, a linear range of 0.8 to 6.25 μg/kg, and substantial recovery, from 85.6 to 108.3%. It showed slight cross-reactivity with another aminoglycoside analog. Strong correlations between the results of established chromatographic time-resolved fluoroimmunoassay and ultra-performance liquid chromatography-tandem mass spectrometry indicated that the established fluoroimmunoassay is a reliable method for rapid onsite detection of streptomycin in milk and it has great potential in food safety monitoring.
研究领域:
食品安全
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免疫学
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分析化学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2022
·
IF 3.898
Shujie Guo,Jiao Du,Fangzheng Yan,Zhi Wang,Jixiao Wang
Polyamide (PA) thin-film composite (TFC) nanofiltration (NF) membrane has extremely broad application prospects in separation of monovalent/divalent inorganic salts mixed solution. However, membrane fouling is the main obstacle to the application of PA, TFC and NF membrane. Streptomycin (SM) is a hydrophilic antibiotic containing a large number of hydroxyl and amino groups. In this work, the NF membrane was prepared via interfacial polymerization (IP) between trimesoyl chloride (TMC) in the organic phase and SM/piperazine (PIP) mixture in the aqueous phase. The NF membrane structure and performance were characterized in detail. The results showed that SM successfully participated in the IP. The negative charge and hydrophilicity of membrane surface were improved. The prepared membrane exhibited good anti-adhesion and anti-bacterial performance. Additionally, when the SM concentration was 2%, the prepared membrane exhibited the optimal permselectivity. The water permeance was 89.4 L·m −2 ·h −1 ·MPa −1 . The rejection of NaCl and Na 2 SO 4 were 17.17% and 97.84%, respectively. The NaCl/Na 2 SO 4 separation factor of the SM2-PIP/TMC membrane in 1000 mg·L −1 NaCl and 1000 mg·L −1 Na 2 SO 4 mixed solution was 40, which was 3.3 times that of PIP/TMC membrane. It indicated that SM2-PIP/TMC demonstrated excellent monovalent/divalent salts separation performance. This work provided an easy and effective approach to preparing anti-fouling NF membrane while possessing superior monovalent/divalent salts separation performance.
研究领域:
材料科学
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环境工程
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生物医学
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DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
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2020
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IF 5.88
Xingyu Zhao,Minmin Chen,Hualin Wang,Li Xia,Min Guo,Suwei Jiang,Qian Wang,Xingjiang Li,Xuefei Yang
Synergistic antibacterial strategies have received growing attention due to their significantly enhanced antibacterial activity. Herein, we demonstrated a synergistic antibacterial strategy based on streptomycin sulfate (SS) loaded polyethylene glycol-MoS 2 /reduced graphene oxide (PEG-MoS 2 /rGO) nanoflakes assisted with near-infrared (NIR). The nanoflakes of PEG-MoS 2 /rGO were ultrasonically exfoliated well from the nanoflowers of PEG-MoS 2 /rGO fabricated by hydrothermal method, which was of antibacterial activity against Staphylococcus aureus and Escherichia coli after loading of SS. Under the irradiation of NIR, the antibacterial activity was significantly enhanced by the synergistic effects of physical damage, protein synthesis inhibition, thermal injury and oxidative stress. Moreover, the cytotoxicity of the nanoflakes was very low. The results suggest the great potential of PEG-MoS 2 /rGO-SS as a photothermal antibacterial agent.
研究领域:
材料科学
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微生物学
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医学
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DOI
PubMed
高影响因子
RSC Advances
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2017
·Vol. 7
·期 61
·38471-38478
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IF 3.108
Jing Zhao,Yuangen Wu,Han Tao,Huayun Chen,Wenping Yang,Shuyi Qiu
A novel colorimetric aptamer sensor (aptasensor) for the detection of streptomycin (STR) is developed based on the peroxidase-like activity of gold nanoparticles (AuNPs) and their interaction with STR-aptamer complex. It is known that AuNPs can easily oxidize substrates in the presence of hydrogen peroxide in a manner identical to peroxidase and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is a commonly used peroxidase substrate with green oxidation product and a characteristic absorption peak at 733 nm. In the absence of STR, STR1 aptamer is adsorbed on the surface of AuNPs, and restrains its catalytic activity due to shield effect of ssDNA sequence against substrates. STR first binds with STR1 aptamer to form an STR-aptamer complex; thus, aptamer sequences do not get absorbed on the surface of the nanoparticles and their peroxidase activity is further greatly enhanced by the STR-aptamer complex, which enables AuNPs to catalyse the oxidation of the substrates. To achieve a higher level of sensitivity, several key parameters of the proposed aptasensor such as pH, concentrations of STR1 aptamer and AuNPs, and incubation and reaction temperature have been investigated. Under optimal conditions, the proposed aptasensor can detect STR in a linear range from 0.1 μM to 0.5 μM with a limit of detection (LOD) as low as 86 nM and exhibits good selectivity. Moreover, further studies also validate the applicability of the proposed aptasensor in milk samples, revealing that it may have enormous potential utility for practical STR detection in food products in the future.
研究领域:
分析化学
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食品科学
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纳米技术
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生物传感器
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Shaochi Wang,Han Wang,Ting Du,Tong Bu,Jingke Xu,Sijie Liu,Xuechi Yin,Yao Wang,Daohong Zhang,Jing Sun,Jianlong Wang
Antibiotic abuse has caused serious health risks for human beings for long. To address the problem, novel and facile detection techniques are highly desired. Here, an effective multiplex immunochromatographic platform (MICP) with synthesis-free and cost-effective merits is established for simultaneous detection of antibiotics on a single immunochromatographic assay (ICA) strip. Adopting crystal violet (CV) as a signal tag for multiplex ICA allows for direct coupling with multiple antibodies in several minutes. By combining CV and ICA perfectly, this convenient strategy offers improvements in convenience, speed, flexibility, and portability, eventually ensuring the optimized effectiveness of this approach. As a result, the established platform is successfully used to detect streptomycin (STR) and chloramphenicol (CAP) with visual detection mode, and the obtained total recoveries of milk and honey real samples changed from 83.82 to 113.38% with total RSD values of 0.48 to 4.15%.
研究领域:
分析化学
·
食品安全检测
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Wanqi Zhang,Zhicong Sun,Yuhang Tian,Yaoting Mou,Yemin Guo,Xia Sun,Falan Li
Streptomycin (STR) is an antibiotic fungicide that is easily enriched in the environment and threatens human health. In this paper, a ratiometric fluorescence aptasensor with silver nanoclusters-skimmed milk powder@zeolitic imidazolate framework-8 (AgNCs-SMP@ZIF-8) fluorescent material as the response signal and carbon quantum dots (CQDs) functionalized with aptamer as the reference signal was prepared. Magnetic graphene oxide selection technology was used to screen high-affinity STR-specific aptamer. The truncation strategy for removing non-binding site bases was based on molecular simulation docking, and it was found that the stem-loop structure formed by the truncated aptamer 52 (Apt52) was used for STR-specific recognition and had a higher STR affinity ( K d = 14.52 nM) compared to the initial 79-nucleotide aptamer. AgNCs was then covered in a protein coating and encapsulated in ZIF-8 to obtain AgNCs-SMP@ZIF-8 with enhanced red fluorescence intensity . The added Cu 2+ rapidly penetrated into ZIF-8 to induce red fluorescence quenching , while the CQDs modified by Apt52 maintained green fluorescence. Hence, the constructed ratiometric fluorescent sensor with Apt52 as the recognizing element can detect STR within 90 s with a detection limit of 0.98 nM. The ratiometric probe proposed has considerable potential for STR residue detection in milk.
研究领域:
分析化学
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生物技术
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纳米技术
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2026
·
IF 4.4
Xinyuan Wang,Yue Liu,Jun Yang,Shaogui Wu
Nitrogen‑doped carbon dots with 24.9% quantum yield were prepared by one‑step hydrothermal synthesis. • Streptomycin was detected by fluorescence over 0–500 μM with a 0.47 μM LOD. • Smartphone G/B analysis enabled visual detection over 0–600 μM with a 0.21 μM LOD. • Water-sample recoveries of 91.8%–116.5% support on-site residue screening. Streptomycin sulfate (SM), a representative aminoglycoside antibiotic, is widely used for the treatment of tuberculosis and Gram-negative bacterial infections, but residues in water and food-related matrices may pose risks to human health. Here, we developed a portable sensing platform for on-site antibiotic residue monitoring. The platform uses carbon dots prepared from 4-aminobutyric acid and 5-amino-o-cresol by a hydrothermal route as a composite fluorescent nanoprobe. The probe showed a quantum yield of 3.8% in water and enabled both visual recognition and quantitative detection of SM. As the SM concentration increased, the probe color shifted from blue-green to yellow, allowing direct visual readout. The fluorescence assay showed a linear response over 0–500 μM with a limit of detection (LOD) of 0.47 μM, whereas the smartphone-assisted colorimetric readout was linear over 0–600 μM with an LOD of 0.21 μM. Selectivity, interference-resistance and spiked-recovery tests in real samples indicate that the method provides a sensitive, stable and matrix-tolerant route for low-cost screening of SM in food and environmental samples. Download: Download high-res image (179KB) Download: Download full-size image
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DOI
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Wanqi Zhang,Lu Han,Yanhao Yin,Yaoting Mou,Yuhang Tian,Zhicong Sun,Yanfang Wu,Dongfei Chen,Yemin Guo,Xia Sun,Falan Li
Excessive streptomycin (STR) residues pose significant risks to dairy product quality and safety. Thus, developing highly sensitive detection methods for STR is essential to safeguard dairy safety and public health. In this study, we present a dual signal amplification lateral flow strip (LFS) using enzyme-assisted cycling and composite probe composed of multiple gold nanoparticles (AuNPs) for STR detection. Through targeted mutation, an optimized aptamer (Apt52-0) with both high affinity for STR and recognition by nicking endonuclease was developed, exhibiting enhanced binding to STR with a K d value of 6.87 nM, compared to the original Apt52. Enzyme-assisted amplification was achieved using the hairpin structure of the Apt52-0, Vent (exo-) DNA polymerase, and Nt.Alwl nicking endonuclease, enabling dual cycling of the target and double-stranded DNA (dsDNA). The cyclic products were then isothermally amplified, and composite probe composed of multiple AuNPs was utilized to construct a dual signal amplification lateral chromatography sensor for STR detection. This aptamer-based LFS achieved a visual limit of detection (LOD) of 25 nM, and a LOD of 17.6 nM analyzed via ImageJ software. The test strip demonstrated promising sensing performance in practical application, with STR recovery rates of 95.8%–103.9% in milk samples. This dual signal amplification-based LFS offers an inexpensive, rapid and sensitive approach to STR detection.
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Bixia Lin,Ying Yu,Yujuan Cao,Manli Guo,Debin Zhu,Jiaxing Dai,Minshi Zheng
s The rapid detection of antibiotic residual in everyday life is very important for food safety. In order to realize the on-site and visual detection of antibiotic, a POCT method was established by using digital image colorimetry based on smartphone. Streptomycin was taken as the analyte model of antibiotics, streptomycin aptamer preferentially recognized analyte, and the excess aptamer hybridized with the complementary DNA to form the dsDNA . SYBR Green I combined with the dsDNA and then emitted obvious green fluorescence, thus the fluorescence intensity decreased with the increasing of streptomycin concentration. Then a smartphone-based device was constructed as the fluorescence readout. The smartphone camera acquired the images of the fluorescence derived from the samples, and the Touch Color APP installed in smartphone read out the RGB values of the images. There was a linear relationship between the G values and the streptomycin concentrations in the range of 0.1–100 µM. The detection limit was 94 nM, which was lower than the maximum residue limit defined by World Health Organization. The POCT method was applied for determining streptomycin in chicken and milk samples with recoveries in 94.1–110%. This method had the advantages of good selectivity , simple operation and on-site visualization.
研究领域:
食品安全
·
生物医学检测
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Yanni Luo,Xuecai Tan,David J. Young,Quanyou Chen,Yuehui Huang,Defen Feng,Chenhao Ai,Yan Mi
In photoelectrochemical sensor (PEC sensor), sensitivity and selectivity are two essential factors which are determined by photosensitive of materials and identification of elements. Herein, a novel PEC aptamer sensor for streptomycin-specific detection was developed, with which the visible-light-active TiO 2 /BiOI/BiOBr heterostructure and aptamers were employed as photoactive material and bio-identification elements, separately. The combination of an appropriate amount of TiO 2 with BiOI/BiOBr enhanced the photocurrent response, and thus is beneficial to the construction of PEC sensors. In addition, the one-pot synthesis of TiO 2 /BiOI/BiOBr has the advantage of being environmentally-friendly. Under optimized conditions, the photocurrent response of aptamer/TiO 2 /BiOI/BiOBr/ITO is linear with SRT concentration from 0.05 to 150 nM, and the detection limit (S/N = 3) is as low as 0.04 nM. This novel PEC sensing strategy provided an ultra-sensitive sensor with high selectivity and stability for SRT detection.
研究领域:
生物传感技术
·
材料科学与工程
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Haishuai Peng,Yuanyuan Hui,Lei Zhang,Fuxin Zhang,Yufang Liu,Jianbin Zheng,Rong Jia,Yuxuan Song,Bini Wang
Streptomycin (STR) residue excess in animal-derived foods can cause severe side-effects, such as ototoxicity and nephrotoxicity. Herein, a novel electrochemical aptasensor based on Ti 3 C 2 and metal–organic frameworks (MOFs) nanomaterials was developed for the rapid detection of STR in milk samples. The nanocomposites were synthesized using an impregnation method, and their morphology and structure were characterized via several characterization methods. The nanocomposites exhibited excellent electrochemical properties. Under the optimal conditions, the aptasensor offered a wide STR detection range from 0.01 to 200 nM with a lower limit of detection of 0.0033 nM. Furthermore, the aptasensor demonstrated remarkable reproducibility, stability, and selectivity and was successfully applied for the STR detection in milk samples with satisfactory recoveries ranging from 94.0% to 105.0%. The proposed method can be considered a new and effective method for the rapid detection of STR. Moreover, it provides a new sensing platform for the detection of other toxins.
研究领域:
分析化学
·
食品科学
·
生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2022
·
IF 4.52
Haishuai Peng,Yuanyuan Hui,Meixue Pu,Ding Yang,Aiqing Zhao,Weizhe Wang,Shan Wu,Bini Wang
Streptomycin (STR) residues in dairy products could cause serious health risks for human or affect the quality of the dairy products fermented by lactic acid bacteria , thereby resulting in economic losses. Thus, STR detection methods need to be developed quickly, simply, and sensitively. In this study, polyethyleneimine (PEI)-functionalized NH 2 -MIL-101(Fe) (PEI-Fe-MOF) was characterized and found to exhibit an enhanced electrochemical characteristics, when compared to Fe-MOFs. [email protected] core-shell NPs could further amplify its electrochemical signal. Based on the PEI-Fe-MOF/ [email protected] NPs nanocomposites, we have developed a novel electrochemical aptasensor to detect STR rapidly. When detected under optimal conditions, a wide linear range between 0.1 and 200 nM was observed for the aptasensor with a detection limit of 0.033 nM. Furthermore, the developed aptasensor has been satisfactorily applied for the rapid STR detection in dairy products with recoveries ranging from 98.2% to 110.1%. Therefore, our method can be considered a new effective method for the rapid detection of STR in dairy products.
研究领域:
食品科学
·
分析化学
·
生物传感技术
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DOI
高影响因子
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