Food & Function
·
2023
·Vol. 14
·期 11
·5417-5428
·
IF 6.1
Mengwei Jia,Huihui Gu,Yongling Lu,Lishuang Lv
Acrolein (ACR) is a highly reactive α,β-unsaturated aldehyde that plays a key role in the pathogenesis of human diseases, such as atherosclerosis and pulmonary, cardiovascular, and neurodegenerative disorders. We investigated the capture capacity of hesperidin (HES) and synephrine (SYN) on ACR by individual and combined means in vitro, in vivo (utilizing a mouse model), and via a human study. After proving that HES and SYN could efficiently capture ACR by generating ACR adducts in vitro, we further detected the adducts of SYN-2ACR, HES-ACR-1, and hesperetin (HESP)-ACR in mouse urine by ultraperformance liquid chromatography-tandem mass spectrometry. Quantitative assays revealed that adduct formation occurred in a dose-dependent manner, and that there was a synergistic effect of HES and SYN on capturing ACR in vivo. Moreover, quantitative analysis suggested that SYN-2ACR, HES-ACR-1, and HESP-ACR were formed and excreted through the urine of healthy volunteers consuming citrus. The maximum excretions of SYN-2ACR, HES-ACR-1, and HESP-ACR were at 2–4, 8–10, and 10–12 h, respectively, after dosing. Our findings propose a novel strategy for eliminating ACR from the human body via the simultaneous consumption of a flavonoid and an alkaloid.
研究领域:
药理学
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毒理学
·
生物化学
·
营养学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2023
·Vol. 14
·期 4
·1971-1988
·
IF 6.1
Ya-Li Wang,Song-Xia Lin,Yuan Wang,Tao Liang,Tao Jiang,Peng Liu,Xiao-Yi Li,Deng-Qin Lang,Qiang Liu,Chun-Yan Shen
Oxidative stress and inflammation play important roles in the development of diabetes mellitus. p-Synephrine, the primary pharmacologically active protoalkaloid in Citrus species, has been popularly consumed as a dietary supplement for weight loss management. However, the effects of p-synephrine on diabetes mellitus and the action mechanisms have not been clearly elucidated. In this study, the in vitro antioxidant effects of p-synephrine were evaluated. The data showed that p-synephrine treatment exhibited significant scavenging effects against DPPH, ABTS and OH radicals and showed high reducing power. Diabetic mice were developed by alloxan injection, followed by p-synephrine administration to investigate its hypoglycemic effects in vivo. The results showed that p-synephrine intervention significantly prevented alloxan-induced alteration in body weight, organ indexes, serum uric acid content and serum creatinine content. Meanwhile, p-synephrine application significantly improved the lipid profiles, superoxide dismutase (SOD) and catalase (CAT) activities and glutathione (GSH) contents in the serum and kidneys of diabetic mice and reduced the malondialdehyde (MDA) content in the serum of diabetic mice. Further assays suggested that p-synephrine treatment improved alloxan-induced decreases of glucose tolerance and insulin sensitivity. Also, p-synephrine supplementation altered histopathological changes in the kidneys and interscapular brown adipose tissues in diabetic mice. In addition, p-synephrine administration inhibited renal inflammation through suppressing tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) gene expression levels, as well as CD45 expression levels. The anti-inflammatory effects were probably involved in the regulation of nuclear factor-κB (NF-κB) activation and mitogen-activated protein kinase (MAPK) phosphorylation. In conclusion, p-synephrine application significantly ameliorated alloxan-induced diabetes mellitus by inhibiting oxidative stress via suppressing the NF-κB and MAPK pathways.
研究领域:
药理学
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内分泌学
·
分子生物学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2023
·
IF 5.1
Peng Zhou,Xiangzhou Li,Jun Zhou,Wensheng Li,Liqun Shen
A molecularly imprinted polymer (MIPs) was prepared based on a computer-aided design, which was used to selectively enrich and purify synephrine (SYN) from the extract waste liquid of Citrus aurantium L. The suitable functional monomers , cross-linkers and reaction systems for preparing MIPs were quickly screened with density functional theory (DFT) calculation to effectively avoid the design blindness of molecularly imprinted material. The synephrine molecularly imprinted polymer (SYN-MIPs) with imprinted holes and magnetic properties were prepared with nano Fe 3 O 4 as the core, and the structure, morphology, magnetic property and adsorption behavior were studied and the interaction mechanism of the adsorption process was clarified by DFT calculations. The saturated adsorption of SYN-MIPs for SYN was as high as 47.38–53.68 mg/g within 298 K to 308 K. The adsorption behavior of SYN-MIPs for SYN was well fit for the Freundlich adsorption and the pseudo-second-order kinetic model. It was worth mentioning that SYN-MIPs showed distinguished re-usability and specific recognition. The imprinting factor and selection factor to SYN were 3.13 and 2.43, respectively. The selective enrichment and purification of SYN from the extract waste liquid of Citrus aurantium L. achieved satisfactirily, and the purity of SYN enriched to 93.73% while the concentration of SYN was only 1.73% in the extract waste liquid. The study provides not only an efficient adsorption separation polymer materials but also a practical for the enrichment and purification process of SYN from the extract waste liquid of citrus plants.
研究领域:
化学工程
·
药物化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）