Toxics
·
2023
·Vol. 11
·期 5
·428
·
IF 4.6
Yanli Man,Tian Sun,Chi Wu,Xingang Liu,Mingyuan He
The risks posed by chemicals in the environment are typically assessed on a substance-by-substance basis, often neglecting the effects of mixtures. This may lead to an underestimation of the actual risk. In our study, we investigated the effects of three commonly used pesticides—imidacloprid (IMI), cycloxaprid (CYC), and tebuconazole (TBZ)—both individually and in combination, using various biomarkers to assess their impact on daphnia. Our findings indicated that the order of toxicity, from highest to lowest, was TBZ, IMI, and CYC, as determined by acute toxicity as well as reproduction. The effects of the ITmix (IMI and TBZ) and CTmix (CYC and TBZ) combinations on immobilization and reproduction were evaluated by MIXTOX, revealing a higher risk of immobilization at low concentrations for ITmix. The effect on reproduction differed depending on the ration of pesticides in the mixture, with synergism observed, which may be caused mainly by IMI. However, CTmix showed antagonism for acute toxicity, with the effect on reproduction depending upon the composition of the mixture. The response surface also exhibited a switch between antagonism and synergism. Additionally, the pesticides extended the body length and inhibited the development period. The activities of superoxide dismutase (SOD) and catalase (CAT) content was also significantly induced at different dosage points in both the single and combination groups, indicating changes in the metabolic capabilities of detoxifying enzymes and target site sensitivity. These findings highlight the need for more attention to be focused on the effects of pesticide mixtures.Keywords:ecotoxicity;pesticide mixture;combined toxicity;MIXTOX;risk assessment
研究领域:
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Zi-Ying Zeng,Dong-Dong Zhou,Shu-Tong Yang,Miao-Miao Su,Zhong-Hua Yang
This work synthesized a novel adsorbent by doping COF and MIL-125(Ti) in the nanoparticles Fe 3 O 4 . The obtained results showed that the doping of COF and MIL-125(Ti) had a positive effect on enhancing the specific surface area and adsorption capacity. Moreover, the adsorption process has been confirmed to follow the Langmuir model and pseudo-second-order kinetic, and the maximum adsorption capacity for tetracycline and tebuconazole was 138.1 mg/g and 87.73 mg/g, respectively. Finally, the adsorbent displayed an excellent adsorption performance in three kinds of actual environmental water samples. The mechanics of the adsorption process mainly involved π-π stacking interaction, hydrogen-bonding interaction, electrostatic interaction, and complexation interaction. In short, the synthesized magnetic adsorbent displayed a good application potential for removing tetracycline and tebuconazole from wastewater.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2022
·
IF 4.966
Huiyuan Wang,Yunpeng Gai,Youfu Zhao,Minhui Wang,Zhonghua Ma
The calcium-calcineurin and high-osmolarity glycerol (HOG) pathways play crucial roles in fungal development , pathogenicity , and in responses to various environmental stresses. However, interaction of these pathways in regulating fungicide sensitivity remains largely unknown in phytopathogenic fungi. In this study, we investigated the function of the calcium-calcineurin signalling pathway in Fusarium graminearum , the causal agent of Fusarium head blight. Inhibitors of Ca 2+ and calcineurin enhanced antifungal activity of tebuconazole (an azole fungicide) against F. graminearum . Deletion of the putative downstream transcription factor FgCrz1 resulted in significantly increased sensitivity of F. graminearum to tebuconazole. FgCrz1-GFP was translocated to the nucleus upon tebuconazole treatment in a calcineurin-dependent manner. In addition, deletion of FgCrz1 increased the phosphorylation of FgHog1 in response to tebuconazole. Moreover, the calcium-calcineurin and HOG signalling pathways exhibited synergistic effect in regulating pathogenicity and sensitivity of F. graminearum to tebuconazole and multiple other stresses. RNA-seq data revealed that FgCrz1 regulated expression of a set of non-CYP51 genes that are associated with tebuconazole sensitivity, including multidrug transporters, membrane lipid biosynthesis and metabolism, and cell wall organization. Our findings demonstrate that the calcium-calcineurin and HOG pathways act coordinately to orchestrate tebuconazole sensitivity and pathogenicity in F. graminearum , which may provide novel insights in management of Fusarium disease.
研究领域:
植物病理学
·
分子生物学
·
真菌遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Jinhua Jiang,Changpeng Zhang,Luyan Wang,Xiangyun Wang,Hongmei He,Shenggan Wu,Xueping Zhao
Limited literature had focused on the combined effect of triazole fungicides on aquatic organisms at environmental concentrations. In this research, difenoconazole (DIF) and tebuconazole (TEB) mixture exhibited additive effect on the acute toxicity to zebrafish embryos. The transcriptomics and metabolomics demonstrated DIF and TEB mixtures at aquatic life benchmark and environmental concentration simultaneously influenced the lipid metabolism, arachidonic acid metabolism, steroid hormone biosynthesis and tryptophan metabolism, but showed diverse response patterns mediating the combined effects on zebrafish embryos after 120 h exposure. The DIF and TEB mixture at aquatic life benchmark caused combined effect on yolk sac resorption and metabolites, was less than the additive effect of individual DIF and TEB. It was found environmental concentration of DIF and TEB caused much lower levels of IFN and IL6, induced higher levels of PGE 2 , l -kynurenine and formylanthranilate in zebrafish larvae, and their binary mixture caused synergistic effect on the accumulation of metabolites in metabolic pathways, which might cause more negative effect and risk on growth in zebrafish later life stages. Results further demonstrated that adding arachidonic acid (AA) increased the transcripts of Pla 2 , Ptgs1 , Cyp19a and Cxcl8b , allayed the accumulation of PLA 2 and 17 β -E 2 , and induced more PGF2 α , IFN and IL6 levels in zebrafish larvae, indicated AA metabolism might play important regulatory roles on hormone synthesis and immune response caused by DIF and TEB mixtures. Current results indicated the risk assessment of mixtures based on single concentration may not precisely estimate the environmental risk and health effect, it is crucially important to consider the multi-concentration combinations, and more attention should be paid to the environmental concentration.
研究领域:
环境毒理学
·
水生生物学
·
分子生物学
·
药理学
·
生态毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Toxics
·
2023
·Vol. 11
·期 4
·326
·
IF 4.6
Xiaoran Ma,Xin Chen,Haonan Hou,Donghui Liu,Xueke Liu,Peng Wang,Zhiqiang Zhou
As two commonly used fungicides, carbendazim and tebuconazole are widely found in the environment and in foods. Studies have reported that these fungicides can induce hepatic oxidative stress and other health risks. Nevertheless, the influences of exposure to carbendazim and tebuconazole at their acceptable daily intake (ADI) doses on hepatic oxidative stress, and the residual distributions in mice remain unclear. To fill these gaps, ICR (CD-1) mice were exposed to carbendazim and tebuconazole at their ADI doses by oral administration for 4 weeks in this study. The results showed that tebuconazole accumulated primarily in the epididymal fat of mice (16.84 μg/kg), whereas no significant residues of carbendazim in the tissues were observed. In addition, exposure to ADI doses of tebuconazole significantly reduced liver coefficients and induced hepatic oxidative stress in mice, including elevating the levels of glutathione and malonaldehyde. However, no significant impacts were observed on the hepatic redox homeostasis in mice after exposure to carbendazim at its ADI dose. The results could be helpful for understanding the exposure risks of carbendazim and tebuconazole in terms of low doses and long term.Keywords:carbendazim;tebuconazole;residue;oxidative stress;acceptable daily intake
研究领域:
毒理学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Bo Zhao,Fang Chen,Kexin Zhou,Manfeng Lin,Lihu Shi,Shican Mi,Haixia Pan,Qiang Yao,Xin Zhao
Microplastics and nanoplastics (NPs) are pollutants of global concern. However, the understanding of the combined effects of NPs and other pollutants in the soil-plant system remains limited, particularly for polyethylene (PE), the primary component of agricultural films. This study investigated the effects of PE NPs (0.5%, w/w), fungicide tebuconazole (Te, 10 mg·kg -1 ), and cadmium (Cd, 4.0 mg·kg -1 ) on the soil-wheat system under single and combined exposures. The synergistic toxicity observed between NPs and Te impacted the nutritional conditions and antioxidant mechanisms of the soil-wheat system. The NPs increased the concentration of Cd in roots and the proportion of bioavailable Cd, exacerbating oxidative stress in wheat and inhibiting biomass. The soil-wheat system responded to stress by upregulating or downregulating pathways related to carbohydrate, amino acid, and sugar metabolism under various treatments. Sixteen functional genes associated with carbohydrate metabolism, amino acid metabolism, energy utilization, and gene repair at KEGG level 3 were employed to sustain microenvironmental homeostasis. Correlation analysis between microorganisms and environmental factors showed that various PGPG played roles in maintaining the health of the soil-wheat system. These results help to elucidate the comprehensive effects of NPs with other pollutants on the soil-plant system and provide new perspectives for toxic mechanisms.
研究领域:
环境科学
·
农业
·
土壤学
·
植物生理学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AUSTRALIAN JOURNAL OF CHEMISTRY
·
2021
·Vol. 74
·期 12
·838-846
·
IF 1.321
Xicheng Xie,Lingyun Li,Lumei Wang,Chaoqiang Pan,Dongwei Zhang,Guoqing Shen,Xicheng Xie,Lingyun Li,Lumei Wang,Chaoqiang Pan,Dongwei Zhang,Guoqing Shen
This paper illustrates the establishment of a colourimetric method for detection of the fungicide tebuconazole (TEB) in aqueous samples based on an unmodified TEB-specific aptamer and gold nanoparticles (AuNPs). In the absence of TEB, the AuNPs are coated with a TEB-specific aptamer and then stably dispersed a NaCl solution of high concentration, leading to a red solution and producing a maximum UV absorption peak at 520 nm. In the presence of TEB, due to the specific high affinity between TEB and the TEB-specific aptamer, the aptamer combines with TEB to form stable compounds, causing the AuNPs to be exposed in the solution and aggregate. The aggregated AuNPs turn the solution from red to blue, presenting a maximum UV absorption peak at 650 nm. Therefore, the concentration of TEB in the system can be quantitatively detected through the changes in absorbance. This TEB selective colourimetric biosensor detects TEB over a linear concentration range of 20 to 400 nM (R = 0.99385) and has a limit of detection (LOD) of 4.13 nM. The average recovery of TEB is 94.9–104.8 % in the application of actual water samples with the relative standard deviations (RSD) ranging from 1.01 to 5.34 %. With considerable sensitivity and selectivity, this aptasensor indicates great potential for TEB detection in aqueous samples.
研究领域:
分析化学
·
环境科学
·
纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Kun Jia,Guilan Chen,Junquan Zeng,Fasheng Liu,Xinjun Liao,Chen Guo,Jiaqi Luo,Guanghua Xiong,Huiqiang Lu
Trifloxystrobin-tebuconazole (TFS-TBZ) is a novel, broad-spectrum fungicide that has been frequently detected in both the environment and agricultural products. However, its adverse effects on aquatic organisms remain unknown. In this study, the adverse effects of ecologically relevant TFS-TBZ concentrations (i.e., 75.0, 112.5, and 150.0 μg/L) on the heart and development of zebrafish were investigated. TFS-TBZ was found to substantially hinder development, inhibit growth, and cause significant abnormity at higher concentrations. Moreover, TFS-TBZ caused severe pericardial edema, heart loop failure, cardiac linearization, and ultra-slow heartbeat, implying that TFS-TBZ might induce congenital heart disease. TFS-TBZ inhibited Notch signaling and increased the intracellular generation of reactive oxygen species, resulting in decreased myocardial cell proliferation and increased apoptosis. The use of sodium valproate and Gadofullerene illustrated the relevance of the Notch signaling system and oxidative stress. Finally, TFS-TBZ exposure conveys severe developmental toxicity to the zebrafish heart. The underlying mechanism is regulation notch mediated‐oxidative stress generation, implying that TFS-TBZ may be potentially hazardous to aquatic organisms in the environment.
研究领域:
环境毒理学
·
水生生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2019
·
IF 5.108
Shuying Li,Hongbing Jiang,Kun Qiao,Wenjun Gui,Guonian Zhu
Silkworm ( Bombyx mori ) is one of the most important economic insects in the world, while pesticides impact its economic benefits. Tebuconazole is a fungicide that has been frequently detected in agriculture systems at concentrations that affect endocrine function in organisms. In the present study, silkworm larvae at different instar stages were exposed to tebuconazole, respectively. Cocoon weight, cocoon shell weight and cocoon shell rate were significantly decreased by 6.8%, 11.8% and 4.4% respectively, after exposure to 0.40 mg/L tebuconazole at 2nd -3rd instar stage. Vacuolization was found in the exposure silkworm under histopathological study at all stages exposures, indicating potential damage to silk gland. Downregulation of genes transcription ( Fibh , Fibl , P25 , Ser2 , Ser3 ) involved with protein synthesis in the silk gland were further observed, and the results showed significant decreasing in mRNA expression among the tebuconazole treatments. Ecdysteroid levels in silkworm were changed with pronounced decreases after exposed to tebuconazole. In contrast, exposure to tebuconazole significantly increased juvenile hormone 1 concentrations and the maximum increasing fold of juvenile hormone 1 was up to 3.73 which was observed at stage I exposure. In addition, co-exposure to 2 and 10 mg/L forskolin able to mitigate tebuconazole-induced downregulate of mRNA expression of Sgf1 in the present study, indicating the potential mechanism of tebuconazole-induced chronic toxicity in silkworm may relative to PI3K/AKT/TORC1/Sgf1 pathway.
研究领域:
昆虫学
·
毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2021
·Vol. 108
·期 2
·267-275
·
IF 2.151
Pan Lixiang,Feng Xiaoxiao,Jing Jing,Zhang Jingcheng,Zhuang Ming,Zhang Yun,Wang Kai,Zhang Hongyan
The mixed application of pesticides and foliar fertilizer has been widely used in the production of cucumber, however, their effects on plant growth and pesticide dissipation are still unclear. In this study, the effects of mixed application of pymetrozine, tebuconazole and foliar fertilizer on the cucumber plant growth and pesticide dissipation were investigated simultaneously. The results show that the mixed use of pymetrozine, tebuconazole, especially adding foliar fertilizer, improved the physiological indexes (i.e., area, nitrogen content and chlorophyll content of the leaves, and root growth) of cucumber plants compared to those with the application of single pesticide. Meanwhile, it can significantly affect the dissipation of pymetrozine even in the slower growth matrices (lower leaves, stems, and plants). The residue of tebuconazole in cucumber plants was affected by the combination of formulation type and foliar fertilizer. This study can provide data for scientifically guiding the mixed application of pesticide and fertilizer.
研究领域:
农业科学
·
植物保护
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2023
·
IF 4.1
Shujing Li,Jing Sun,Yue Gao,Aihua Zou,Jiagao Cheng
BACKGROUND Prothioconazole (PTC) is one of the leading fungicide products worldwide. However, excessive use of PTC facilitates the development of resistance. Pesticide compounding technology plays an important role in reducing pesticide resistance. Microspherization technology for the construction of pesticide dual-loaded systems has recently provided a new direction for researching novel and efficient pesticide formulations. In this study, prothioconazole-tebuconazole @ polylactic acid microspheres (PTC-TBA@PLA MS) were constructed by combining these two technologies. RESULTS The final PTC-TBA@PLA MS was selected by an orthogonal method, which were uniformly spherical with smooth surface. The resultant drug loading (DL) and average particle size of PTC-TBA@PLA MS were 31.34% and 22.3 μm, respectively. A PTC-TBA@PLA MS suspending agent (SC) with a high suspension rate of 94.3% was prepared according to the suspension rate, dumping ability and stability. Compared with a commercial SC, the PTC-TBA@PLA MS SC had a larger cumulative release and better interfacial properties. Biological experiments showed that PTC-TBA@PLA MS SC had an obviously improved bactericidal effect than the commercial SC. CONCLUSION The constructed PTC-TBA@PLA MS system detailed here is expected to reduce the risk of resistance and the frequency of pesticide use while enhancing fungal control. This article is protected by copyright. All rights reserved.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 29
·期 1
·163
·
IF 4.6
Siwei Wang,Yanping Liu,Manshan Zhu
The present study describes the development of a highly effective approach for determining the residue distribution and dissipation of trifloxystrobin and tebuconazole, and their risk assessment in brown rice, husk, straw, and grain using high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS). The current study provides considerable novel information regarding the safe utilization of a mixture of trifloxystrobin and tebuconazole in paddy production. The samples demonstrated a range of mean recoveries between 72% and 86%, with a 1.1–9.2% relative standard deviation (RSD). The limits of quantification (LOQ) and half-lives (t1/2) for brown rice, husk, straw, and grain were, respectively, established to be 0.001–0.01 mg/kg and 4.1–7.7 days. The concentrations of terminal residues in the brown rice, husk, straw, and grain were, respectively, found to be 0.02–0.05, 0.03–0.11, 0.02–0.07, and 0.02–0.05 mg/kg after being treated twice at 168.75 g a.i./ha with 21 and 28 days of pre-harvest intervals (PHIs). Trifloxystrobin and tebuconazole presented a non-negligible chronic risk to human subjects, as evidenced by a risk quotient (RQ) value of less than 1.Keywords:trifloxystrobin and tebuconazole;paddy;residue distribution;risk assessment;dissipation dynamics
研究领域:
农业科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）