JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Na Wu,Linyu Wang,Yi Xie,Yan Du,Yonghai Song,Li Wang
Riboflavin (RF) is one of the necessary vitamins. If human body lacks RF, it will lead to inflammation and dysfunction of mouth, lips and skin. Thus sensitive and accurate determination of RF is necessary. Here, an electroactive covalent-organic framework nanobelt (COF TFPB-Thi ) with thickness of 1.4 nm was prepared by amine-aldehyde condensation reaction between thionine and 1, 3, 5-tris (p-formylphenyl) benzene, which was then grown vertically on three-dimensional porous carbon derived from kenaf stem (3D-KSC) for double signal ratiometric electrochemical detection of RF. The resulted 3D-KSC/COF TFPB-Thi showed two reduction peaks at −0.08 V and −0.23 V, which came from the reduction of COF TFPB-Thi and the conjugated structure of COF TFPB-Thi , respectively . In the presence of RF, those RF molecules near the electrode surface were oxidized at 0.6 V. Then some oxidized RF (RF ox ) adsorbed on COF TFPB-Thi would oxidize COF TFPB-Thi into COF TFPB-Thi (ox) while other RF ox adsorbed on 3D-KSC kept unchanged. When the potential was scanned from 0.6 V to −0.6 V, both COF TFPB-Thi (ox) and RF ox adsorbed on 3D-KSC were reduced at −0.08 V and −0.45 V accordingly, while the reduction peak of −0.23 V of the conjugated structure of COF TFPB-Thi kept constant. When j -0.45 /j -0.23 was used as the response signal, the detection limit was 44 nM and the linear range was 0.13 μM −0.23 mM. By using j -0.08 /j -0.23 as the response signal, a detection limit of 90 nM and a linear range of 0.30 μM-0.23 mM (S/N = 3) were obtained. By using double signals, the measurement results can be corrected to make the results more accurate and reliable. The sensor also showed good selectivity, reproducibility and stability, which provided a good application prospects.
研究领域:
分析化学
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材料科学
·
电化学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 114
·112981-112987
·
IF 3.289
Xinai Zhang,Chenyong Huang,Yuxiang Jiang,Jianzhong Shen,Ping Geng,Wen Zhang,Qilin Huang
Glycans that attached to glycoproteins on cell surfaces are known to be functionally important and they have potential diagnostic values. The development of effective methods to analyze glycan expression on living cell surface is desirable for understanding their functions in disease progression and providing a powerful tool for early diagnosis. In this paper, thionine-bridged multiwalled carbon nanotube/gold nanoparticle (MWCNT/Th/AuNP) as a mediator/nanomaterial composite was synthesized to develop an electrochemical biosensor for glycan assay on living cancer cells. The MWCNT/Th/AuNP composite was fabricated by binding AuNPs to the surface of Th-coated MWCNTs, in which thionine acted as a linker to enable the negatively charged AuNPs to bind to the anionic MWCNT surface. When compared with conventional nanomaterial-based electrochemical biosensors, the MWCNT/Th/AuNP-modified electrode contained thionine as an electron mediator and simplified the electrochemical process based on enzymatic catalysis for signal amplification. Under a sandwich-type assay format, the MWCNT/Th/AuNP-based biosensor facilitated highly sensitive and specific detection of mannose on human liver and prostate cancer cells and offered great promise for the analysis of other glycans on living cells by utilizing a greater variety of lectins.
研究领域:
电化学
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生物传感
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纳米技术
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
BIOELECTROCHEMISTRY
·
2019
·
IF 4.474
Xueyan Li,Xianwen Kan
In this work, a novel electrochemical sensing platform was designed and fabricated by the modification of boronic acid functionalized carbon nanodots (B-CNDs) and poly(thionine) (pTHI) on an electrode surface. B-CNDs can not only accelerate electron transfer but also covalently interact with cis-diol groups of dihydronicotinamide adenine dinucleotide (NADH) through functionalized boronic acid groups. Meanwhile, pTHI served as an inner reference element to provide a built-in correction, which enabled the sensor to detect NADH with high accuracy and reliability based on a ratiometric signal (∆I NADH /∆I THI ). The electrochemical experimental results demonstrated that the ratiometric strategy-based sensor possessed good selectivity and high sensitivity. A linear range of 5.0 × 10 −7 - 2.0 × 10 −4 mol/L for NADH detection was obtained with a limit of detection of 1.5 × 10 −7 mol/L. The sensor has been applied to analyze NADH in human serum samples with satisfactory results. The simple and effective ratiometric strategy reported here can be further used to prepare electrochemical sensors for selective, sensitive, and reliable detection of other cis-diol compounds.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2023
·Vol. 36
·期 3
·e202300324
·
IF 3
Kankan Chen,Jiawei Xu,Jingjing Song,Zhonggang Liu,Zheng Guo
The ratiometric electrochemical sensors have been highly explored to minimize system errors and environmental interference. However, it is extremely desirable on the development of an effective ratiometric electrochemical sensor in a facile approach without the complicated assembly process. In this work, an effective internal reference probe was coupled based on the polymerized thionine (pTh) via a one-step electrodeposition method. The ratiometric electrochemical sensing platform was applied for 5-hydroxytryptamine (5-HT) detection, where the internal reference probe, Th, presented the robust redox signal and can be clearly distinguished from the oxidation signal of 5-HT. The proposed pTh modified GCE presented the outstanding electrochemical performance on sensing 5-HT in a wide concentration range with a detection limit of 8.4 nΜ. Further, the fabricated sensor was efficiently employed for the detection of 5-HT in various human serum samples and the obtained results were consistent with the reported range in serum samples. This work offers the great possibility on the fabrication of efficient ratiometric sensors in a facile approach and presents great application prospects in clinical diagnosis.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Shasha Pang,Xianwen Kan
In this work, a facile, one-pot, and green synthetic approach was proposed to prepare a novel nanocomposite of nitrogen doped graphene-thionine-gold nanoparticles (NG-Thi-AuNPs), which was then modified on electrode to fabricate an electrochemical sensor for diethylstilbestrol (DES) accurate and reliable detection based on ratiometric strategy. The synthesized NG-Thi-AuNPswas characterized by transmission electron microscope, scanning electron microscope, Fourier transform infrared spectrum, and X-ray diffraction. Due to the merits of good conductivity and high electroactive surface of NG and AuNPs, as well as the synergistic interaction toward DES, the nanocomposite showed high sensitivity for DES detection. Moreover, the modified Thi exhibited a constant current response regardless how the DES concentration changed, which made Thi to be a good inner reference to improve the accuracy and reliability of the sensor. Thus, a ratiometric signal (Δ i DES /Δ i Thi ) was measured and calculated for DES detection with a wide linear range and a low limit of detection of 5.0 × 10 −8 - 8.0 × 10 −5 mol/L and 9.6 × 10 −9 mol/L, respectively. Due to the excellent conductivity and high electrocatalytical activity of the nanocomposite, the sensor was employed for the sensitive detection of H 2 O 2 with the linear range of 5.0 × 10 −7 - 1.0 × 10 −2 mol/L. With the good anti-interference, reproducibility, and stability, the prepared sensor was successfully used for real samples determination.
研究领域:
分析化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Xiu-Xiu Dong,Li-Peng Yuan,Yi-Xin Liu,Min-Fu Wu,Bo Liu,Yuan-Ming Sun,Yu-Dong Shen,Zhen-Lin Xu
Progesterone (P4) is a kind of hormone that can cause neuropathic disturbances in humans when the concentration overpasses a certain degree. In this work, an electrochemical immunosensor capable of detecting P4 sensitively and selectively was developed. Thionine-graphene oxide (Thi-GO) composites with excellent biocompatibility were synthesized and coated to a clear glassy carbon electrode. P4 coating antigen (P4-OVA) was immobilized to the electrode, then sample as well as biotinylated antibody (biotin-P4 Ab) were added. The free P4 can compete with P4-OVA for binding to biotin-P4 Ab. After the further addition of streptavidin-HRP, H 2 O 2 was introduced to develop electrical signal for quantitative determination of P4. After careful optimization of assay conditions, the proposed immunosensor showed a linear range from 0.02 to 20ngmL −1 for P4 in milk samples. The averaged recoveries from spiked samples ranged from 84.0% to 102.0%, which correlated well with standard HPLC-MS/MS. The biosensor also showed good specificity, reproducibility and stability, indicating its potential application in monitoring of P4 in a simple and low cost manner.
研究领域:
分析化学
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生物传感器
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电化学
·
纳米材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2014
·
IF 4.517
Shan Liu,Maochao Wei,Xiangli Zheng,Shuai Xu,Changli Zhou
We are just beginning to exploit the fascinating potential of thionine , called electrochemical probe that can selectively recognize specific polycyclic aromatic hydrocarbons (PAHs), as tools for the detection of tricyclic aromatic hydrocarbons phenanthrene (PHE) and anthracene (ANT). A novel electrochemical sensing platform by modification of electroactive thionine functionalized graphene onto glass carbon electrode (Th/GRs/GCE) surface was constructed. The immobilized thionine showed a remarkable stability, which may benefit from the π–π stacking force with graphene. Under optimum conditions, the proposed electrochemical sensor exhibited high sensitivity and low detection limit for detecting PHE and ANT. The total amount of PHE and ANT could be quantified in a wide range of 10 pM–0.1 μM with a good linearity ( R 2 = 0.9979) and a low detection limit of 0.1 pM ( S / N = 3). Compounds which possess one or two benzene rings or PAHs with more than three rings, such as benzene, naphthalene (NAP), benzo[a]pyrene (BaP) and pyrene (PYR) show little interference on the detection. Consequently, a simple and sensitive electrochemical method was proposed for the determination of PHE and ANT, which was used to determine PHE and ANT in waste water samples. The electrochemical method provides a general tool that complements the commonly used spectroscopic methods and immune method for the detection of PAHs.
研究领域:
分析化学
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环境监测
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电化学传感器
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分子识别
·
污染物检测
✓ 已验证
DOI
高影响因子
RUSSIAN JOURNAL OF ELECTROCHEMISTRY
·
2024
·Vol. 59
·期 12
·1151-1161
·
IF 1.1
Biao Jin,Liu Shanshan,Jin Dongri
The design of the electrode materials for electrochemical sensors is crucial for the simultaneous fast detection of ascorbic acid (AA) and uric acid (UA). A hierarchical porous carbon material (KACM) was prepared via high-temperature calcination and KOH activation using azalea (a species of rhododendron) biomass as the carbon source. KACM and thionine (Thi) were simultaneously assembled on a glassy carbon electrode (GCE) to construct a porous carbon/thionine ratiometric electrochemical biosensor for the detection of ascorbic acid and uric acid. Electrochemical evaluation showed that the Thi/KACM/GCE has good electrochemical performance, strong catalytic activity toward AA and UA oxidation, and good separation of the two oxidation peaks. Under the optimized conditions, Thi/KACM/GCE has a wide linear range (0.05–9 mM) and low detection limit (6.4 μM for AA and 10 μM for UA). Additionally, the biosensor exhibited good selectivity, stability, and reproducibility. Thi/KACM/GCE was used for the simultaneous detection of AA and UA in human urine with 99.4–101.0% recovery of the compounds. This study reports for the first time the use of azaleas in electrochemical biosensors, and the results show that small changes in the structural properties of biomass materials can affect their electrochemical properties.
研究领域:
电化学
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生物传感器
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分析化学
·
纳米材料
·
环境与生物医学分析
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Xiaoli Qin,Yan Yin,Huijing Yu,Wenjuan Guo,Meishan Pei
An ultrasensitive electrochemical aptasensor for the quantitative detection of kanamycin antibiotic was fabricated based on a novel signal amplification strategy. This aptasensor was developed using thionine functionalized graphene (GR-TH) and hierarchical nanoporous (HNP) PtCu alloy as biosensing substrates for the first time. HNP-PtCu alloy with controllable bimodal ligament/pore distributions was successfully prepared by two-step dealloying of a well-designed PtCuAl precursor alloy combined with an annealing operation. GR-TH composite was synthesized by one-step reduction of graphene oxide (GO) in TH solution. Greatly amplified sensitivity was achieved by using GR-TH/HNP-PtCu composite owing to its large specific surface and good electron-transfer ability. Under the optimized conditions, the proposed aptasensor exhibited a high sensitivity and a wider linearity to kanamycin in the range 5×10 −7 –5×10 −2 μg mL −1 with a low detection limit of 0.42 pg mL −1 . This aptasensor also displayed a satisfying electrochemical performance with good stability, selectivity and reproducibility. The as-prepared aptasensor was successfully used for the determination of kanamycin in animal derived food.
研究领域:
分析化学
·
生物传感技术
·
食品安全检测
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Huijun Guo,Jie Chen,Siqi Li,Yifei Qi,Guoxin Wang,Yue Zhang
In this work, a ratiometric double signals electrochemical sensing platform was constructed for creatinine (Cre) analysis employing silver nanoparticles (AgNPs)@ MXenes/thionine acetate (THAT) composites. The synthesis of MXenes/THAT composites was utilized to modify on the surface of a screen-printed electrode (SPE) via dripping coating, followed by the electro-deposition of AgNPs. While the anodic current of the AgNPs decreased in the presence of Cre due to the competive reaction for forming metal complexes, the current of THAT remained invariant, which attained a ratiometric response. Under optimized conditions, the response current ratio ( I Ag /I THAT ) decreases while the concentration of Cre increased linearly between 0 ∼ 1.2 mM, with 0.01 μM as a adaptive detection limit. It provided a new sensing platforming for employing double-signals nanostructures with remarkable stability and sensitivity to design ratiometic sensors for analysis of Cre in human urine samples with real-time.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 6
·1-8
·
IF 5.479
Liu Xinyan,Deng Keqin,Wang Hao,Li Chunxiang,Zhang Shaowei,Huang Haowen
Gold nanoparticles (AuNPs) with a size of ~3 nm were placed on a thionine-multiwalled carbon nanotube (Thi-CNTs) conjugate to form a novel AuNP-Thi-CNTs nanocomposite. Its morphology and composition were characterized by transmission electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The nanocomposite was placed on an electrode and used as a redox-active signaling interface to fabricate a ratiometric electrochemical aptasensor for 17β-estradiol (E2). The potentiostatic insertion method was applied to insert an aptamer against E2 into a thin alkane monolayer to warrant an adequate distance between aptamers. The aptamer against E2 acts as both a collector and separator to specifically bind E2. The electrode displays two peak signals (at +0.50 V vs. SCE for E2; and at −0.32 V for Thi) which increase and decrease, respectively, in the 12 pM to 60 nM E2 concentration range. Therefore, the current ratio can be used to reliably, reproducibly, and sensitively quantify the concentration of E2.
Schematic presentation of a novel AuNP-Thi-CNTs nanocomposite. AuNP-Thi-CNTs showed good electrocatalytic oxidation to E2. AuNP-Thi-CNTs was used as self-redox signal interface to fabricate aptasensor. Dual signals of extrinsic E2 and inner Thi was applied to monitor the concentration of E2.
研究领域:
分析化学
·
纳米技术
·
电化学
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2021
·Vol. 146
·期 14
·4557-4565
·
IF 4.616
Xiangyu Ma,Kun Qian,Onome Ejeromedoghene,Martha Kandawa-Schulz,Wei Song,Yihong Wang
Taking a composite of a nanomaterial and a signal molecule as a substrate material can provide a label-free electrochemical platform. Besides, the nanomaterial with a high catalytic activity towards the signal molecule can improve the sensitivity of the platform. Herein, a thionine functionalized Fe–N–C nanocomposite was employed as the substrate. Firstly, the electrocatalytic activity of Fe–N–C towards the electroreduction of thionine was explored. Then, an immobilization-free and label-free electrochemical platform for the determination of microRNA-21 based on Fe–N–C–thionine/Fe3O4@AuNPs was constructed. A magnetic glassy carbon electrode (MGCE) was used to keep the magnetic Fe–N–C–thionine/Fe3O4@AuNPs modified onto the surface of the MGCE. Fe–N–C and Fe3O4 nanoparticles can co-catalyze the electroreduction of thionine and a strong electrochemical reduction signal of thionine could be realized in the differential pulse voltammetry (DPV) test. Also, a catalytic hairpin assembly (CHA) reaction was utilized to enhance the sensitivity of the developed electrochemical biosensor. Besides, the developed biosensor shows excellent specificity and reproducibility in the test of human serum samples, indicating its wide application prospects in the early-stage diagnosis of tumors.
研究领域:
生物传感技术
·
分子诊断
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）