RSC Advances
·
2026
·Vol. 16
·期 9
·8166-8177
·
IF 6.1
Yifei Meng,Xuan Ou,Zhenghui Liu,Shun Yao,Chuxiao Hu,Qiuchi Yu,Wenjie Xia
Achieving sustainable and cost-effective production and modulating the structure of biosurfactants is increasingly obtaining attention due to carbon neutrality and eco-safety. Nanotechnology-assisted approaches have been emerging as a promising strategy for this purpose. Here, we investigated the effect of metal oxide nanoparticles on the yield and molecular structure of trehalose lipids produced by Rhodococcus erythropolis WJ-2. Sunflower oil was identified as the optimal carbon source against other oils, yielding 12.60 g L−1. Subsequent optimization of four key medium components (sunflower oil, ZnSO4, (NH4)2SO4, and yeast extract) using a Box–Behnken design elevated the baseline yield to 24.75 g L−1. Interestingly, the trehalose lipid yield was further enhanced to 28.12 g L−1 compared to the optimized medium without NPs when supplemented with 0.5 g per L Fe2O3 NPs (≈20 nm). Furthermore, structural diversity analyzed by FTIR and HPLC-MS showed the obvious m/z value shifts of trehalose lipids with NPs comprising longer hydrophobic fatty acid chains and more unsaturated fatty acid derivatives. In the absence of Fe2O3 NPs, the main m/z peaks were detected at m/z 496, 694, 877, and 1044 (relative abundances: 14.19%, 27.08%, 44.18%, 14.55%), corresponding to short-to-medium-chain fatty acid derivatives (e.g., methyl hexanoate, methyl octanoate). In contrast, the dominant peaks shifted to m/z 673, 807, 874, and 1043 (relative abundances: 22.21%, 18.39%, 47.64%, 11.76%) with Fe2O3 NP supplementation. These findings suggest that the unique redox properties and high surface area of Fe2O3 nanoparticles could enhance the yield and modulate the structures to enable the tailored biosynthesis of glycolipids by influencing key-enzyme activity and metabolic flux. This nanoparticle-assisted fermentation strategy offers a promising route for the scalable production of designer biosurfactants for diverse industrial applications.
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高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Ruipu Xin,Suijian Qi,Chaoxi Zeng,Faez Iqbal Khan,Bo Yang,Yonghua Wang
In this study, the natural deep eutectic solvents (NADESs) based on trehalose and choline chloride have been prepared to enhance the protein thermostability. The results of fourier transform infrared spectroscopy and 1 H nuclear magnetic resonance spectroscopy suggested that there were intensive hydrogen-bonding interactions between trehalose and choline chloride in TCCL3-DES and TCCL3-DES75. The physicochemical properties of TCCL3-DES and TCCL3-DES75 were investigated in the temperature range of 293.15–363.15 K. Our results revealed that the thermostability of lysozyme , a model protein used in this study was dramatically increased in TCCL3-DES75, as evidenced by the disappearance of the denaturing peak from their Differential Scanning Calorimetry (DSC) traces. The results of circular dichroism (CD) experiments further demonstrated that the lysozyme in TCCL3-DES75 unfolded partially at 90 °C and recovered to the initial structure at 20 °C. The study suggests that TCCL3-DES75 might be a potential solvent for stabilizing proteins.
研究领域:
生物化学
·
分子生物学
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Tongchao Su,Youtong Zhang,Wenkai Du,Jie Zeng,Haiyan Gao
The trehalose (TL) was modified with sodium alginate, starch, and the effects of modified trehalose particle on the gluten protein and steamed bread properties were investigated. The results showed that for dough frozen after 30 d, the addition of 0.8 % modified TL significantly increased water-holding capacity by 12.12 %, improved the freezing rate, and the change of binding water could be better controlled. Besides, the α-helix content and random coil content of gluten protein was increased and decreased after adding modified TL, respectively, improving the ordered structure of wheat gluten protein and maintaining its aggregation characteristics and structural stability to some extent. Meanwhile, the brightness, water-holding capacity and specific volume of steamed bread was increased after adding 0.8 % modified TL, which improved and maintained the normal texture and internal structure of steamed bread. The results can provide a basis for the application of trehalose in flour products.
研究领域:
食品科学
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分子生物学
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Meigui Huang,Lixia Cong,Ruifeng Ying,Mehraj Ahmad,Gang Hao,Khizar Hayat,Ahmad Mohammad Salamatullah
The intense bitterness of quercetin poses a challenge to its utilization in the food industry. To address this issue, three anionic polysaccharides (carrageenan, pectin, and trehalose) were individually incorporated to fabricate polysaccharide-coated liposome nanocarriers. Electronic tongue analysis revealed a significant decreasing bitterness value (10.34 ± 0.07 mV, sensory score 1.8 ± 0.2, taste weak bitter) in quercetin-loaded nanoliposomes , compared with the bitterness value of quercetin aqueous solution (14 ± 0.01 mV, sensory score 7.3 ± 0.3, taste strong bitter). Furthermore, the polysaccharide-coated nanoliposomes exhibited an even greater capacity to mask the bitterness of quercetin, with carrageenan coated nanoliposomes demonstrating the most pronounced effect. The superior bitter masking ability of carrageenan coated nanoliposomes can be attributed to its high charge and viscosity. In sensory evaluations, gummy incorporated with carrageenan-coated nanoliposomes received the highest ratings, exhibiting enhanced overall palatability and antioxidant activity . This study offers insights into expanding the use of bitter nutrients in food applications and paves the way for more appealing and healthful food products.
研究领域:
食品科学
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纳米技术
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药剂学
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2026
·
IF 5.9
Xiaorong Gou,Jiayi Li,Qianyuan Yu,Xinxin Hao,Shiyu Li,Yiyi Zhang,Hong Deng,Hua Guo,Xue Yang,Weiqi Zhang
Trehalose (Tr) is a naturally occurring disaccharide extensively used as a stabilizing and cryoprotective agent for protein and cell preservation. Owing to its unique bioactivities, Tr-based biomaterials have recently emerged as a promising class of functional materials for biomedical applications. In this work, we reveal that Tr can protect lysosomes from the damage caused by verteporfin (VP), a clinically used photosensitizer. Inspired by this finding, a Tr nanogel (NG) ionically crosslinked with ferric ion was designed for the enhanced photodynamic therapy (PDT) of VP using a murine 4T1 breast cancer model. The constructed Tr/VP NG demonstrates a hydrodynamic diameter of 157.1 ± 2.8 nm, effective cellular uptake and a light-induced reactive oxygen species (ROS) generation in cells. Compared with free VP, the Tr/VP NG exhibits a comparable phototoxicity but with relieved dark toxicity in vitro , which could be evidenced by a less lysosomal leakage in cells and a lower hemolytic activity. Moreover, the Tr/VP NG mediates an elongated tumor accumulation of VP and meanwhile significantly improves antitumor PDT efficacy in vivo . This Tr/VP NG highlights both the feasibility of lysosome protection to minimize undesired dark toxicity of VP and a facile approach for engineering Tr-based materials.
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高影响因子
PARASITOLOGY RESEARCH
·
2026
·
IF 1.9
Hou Jiaming,Ruan Rulin,Zhang Ting,Yang Yongqing,Xue Han,Song Mingxin,Han Caixia
In eukaryotes, with the exception of mammals, trehalose fulfills multiple biological functions, including energy provision and stress tolerance. Trehalose-6-phosphate phosphatase (TPP) is a key enzyme in the trehalose biosynthetic pathway, rather than a trehalose synthase per se. Loss of TPP function or knockdown of its expression impairs trehalose synthesis, which in turn leads to developmental abnormalities or lethality in nematodes. These observations suggest that TPP represents a potential target for the development of anti-parasitic therapeutics. This study focused on the cloning, expression, and characterization of Trichinella spiralis TPP (TsTPP). A recombinant plasmid was constructed to express and purify the recombinant TsTPP protein (rTsTPP), whose immunogenicity was confirmed using positive serum from Trichinella spiralis ( T. spiralis )-infected mouse. The expression of TsTPP at different developmental stages of T. spiralis was determined by immunofluorescence assay. The specific TPP inhibitor N-(phenylthio) phthalimide (NPP) was employed to evaluate its lethal effect on T. spiralis muscle larvae (ML) in vitro. Furthermore, molecular methods such as qPCR and Western blot were used to detect the transcription and protein expression levels of TsTPP upon NPP treatment, aiming to identify new drug targets against T. spiralis . We successfully constructed the recombinant plasmid and obtained rTsTPP. Positive serum assays demonstrated the immunogenicity of rTsTPP, and we used indirect immunofluorescence assay (IFA) to determine its localization across different developmental stages of T. spiralis . Distinct distribution patterns were observed: TsTPP was diffusely distributed throughout the body of newborn larvae (NBL), accumulated in the intestinal glands, midgut ampulla and genital primordia of muscle larvae (ML), and localized to the mid and posterior intestine of adult worms (AW). To verify the lethal effect of NPP in vitro, we treated ML with NPP at concentrations of 12.5, 25, 50, 100, and 200 µg/mL for 24, 36, and 48 h, and evaluated its lethal effect by statistical analysis of worm morphology. The experimental results showed that the mortality rate of ML gradually increased with increasing NPP concentration and culture time. When the concentration was 200 µg/mL, the survival rate of ML was less than 50%. Furthermore, when treated with NPP at concentrations of 12.5, 25, 50, 100, and 200 µg/mL for 24 h, the transcription levels of the TsTPP gene decreased by 19%, 23%, 30%, 38% (all p < 0.05), and 57% ( p < 0.01), respectively; after 36 h of treatment, the transcription levels of TsTPP gene were reduced by 18% ( p < 0.05), 23%, 35%, 39%, and 60% (all p < 0.01); after 48 h of treatment, the transcription levels of TsTPP gene decreased by 19%, 23%, 42%, 42%, and 65% (all p < 0.01), respectively. N-(phenylthio) phthalimide (NPP) exhibits significant lethal activity against T. spiralis by inhibiting the trehalose synthesis pathway, providing basic data for future NPP as a new clinical drug target.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Yan Song,Fengming Gu,Yijiangcheng Li,Weihong Zhou,Fu-an Wu,Jun Wang,Sheng Sheng
The general cutworm, Spodoptera litura (Lepidoptera: Noctuidae) is a worldwide destructive omnivorous pest and the endoparasitoid wasp Meteorus pulchricornis (Hymenoptera: Braconidae) is the dominant endoparasitoid of S. litura larvae. Trehalase is a key enzyme in insect trehalose metabolism and plays an important role in the growth and development of insects. However, the specific function of trehalase in parasitoid and host associations has been less reported. In this study, we obtained two trehalase genes ( SlTre1 and SlTre2 ) from our previously constructed S. litura transcriptome database; they were highly expressed in 3rd instar larvae. SlTre1 was mainly expressed in the midgut, and SlTre2 was expressed highest in the head. SlTre1 and SlTre2 were highly expressed 5 days after parasitization by M. pulchricornis . Treatment with the trehalase inhibitor validamycin A significantly inhibited the expression levels of SlTre1 and SlTre2 , and the trehalase activity. Besides, the content of trehalose was increased but the content of glucose was decreased 24 h after validamycin A treatment in parasitized S. litura larvae. In addition, the immune-related genes in phenoloxidase (PO) pathway and fatty acid synthesis-related genes in lipid metabolism were upregulated in parasitized host larvae after validamycin A treatment. Importantly, the emergence rate, proportion of normal adults, and body size of parasitoid offspring was decreased in parasitized S. litura larvae after validamycin A treatment, indicating that validamycin A disrupts the trehalose metabolism of parasitized host and thus reduces the fitness of parasitoid offspring. The present study provides a novel perspective for coordinating the application of biocontrol and antibiotics in agroecosystem.
研究领域:
昆虫学
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生物化学
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分子生物学
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寄生虫学
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农业生态学
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Qian Wu,Liying Zhu,Qing Xu,He Huang,Ling Jiang,Shang-Tian Yang
Fermentations employing anaerobes always suffer from the restriction of stringent anaerobic conditions during the production of bulk and fine chemicals. This work aims to improve the oxidative stress tolerance of C. tyrobutyricum CCTCC W428, an ideal butyric-acid-producing anaerobe, via the introduction of trehalose biosynthesis capability. Compared with the wild type, the engineered strain showed a wider substrate spectrum, an improved metabolic profile, and a significantly increased specific growth rate upon aeration and acid challenge. Molecular simulation experiments indicated that CoA transferase maintained its native folded state when protected by the trehalose system. Furthermore, qRT-PCR was combined assays for acid-related enzyme activities under various conditions to verify the effects of trehalose. These results demonstrate that introducing a trehalose biosynthetic pathway, which is redundant for the metabolism of C. tyrobutyricum, can increase the robustness of the host to achieve a better oxidative resistance.
研究领域:
微生物学
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生物技术
·
代谢工程
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高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Xiao Huang,Taisong Pan,Jian Shao,Qianwan Qin,Ming Li,Weichang Li,Wei Sun,Yuan Lin
The unsatisfactory performance of Zn metal anodes significantly impedes the commercial application of aqueous zinc-ion batteries (AZIBs). Herein, we introduce a trace amount of a multifunctional trehalose additive to enhance the stability and reversibility of Zn metal anodes. The trehalose additive exhibits a stronger Zn2+ ion affinity due to abundant lone-pair electrons, disrupting hydrogen bonds in H2O, regulating solvation structures, and tuning the Zn–electrolyte interface. Consequently, the Zn metal anode demonstrates a remarkable Coulombic efficiency of 99.80% and a cycle stability exceeding 4500 h at 1 mA cm–2. Even under stringent conditions of 10 mA cm–2, the Zn metal anode maintains a cumulative capacity of 2500 mA h cm–2 without a short circuit. Furthermore, Zn//Zn symmetric batteries exhibit excellent low-temperature cycle performance (over 400 h at −10 °C). As a proof of concept, assembled Zn//NH4V4O10 and Zn//MnO2 pouch cells demonstrate an improved electrochemical performance. This work presents an electrolyte additive strategy for achieving stable zinc anode operation in AZIBs.
研究领域:
电化学
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材料科学
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高影响因子
MOLECULES
·
2022
·Vol. 27
·期 11
·3395
·
IF 4.927
Changli Ma,Taisen Zuo,Zehua Han,Yuqing Li,Sabrina Gärtner,Huaican Chen,Wen Yin,Charles C. Han,He Cheng
The atomic picture of cellulose dissolution in alkali/urea aqueous solution is still not clear. To reveal it, we use trehalose as the model molecule and total scattering as the main tool. Three kinds of alkali solution, i.e., LiOH, NaOH and KOH are compared. The most probable all-atom structures of the solution are thus obtained. The hydration shell of trehalose has a layered structure. The smaller alkali ions can penetrate into the glucose rings around oxygen atoms to form the first hydration layer. The larger urea molecules interact with hydroxide groups to form complexations. Then, the electronegative complexation can form the second hydration layer around alkali ions via electrostatic interaction. Therefore, the solubility of alkali aqueous solution for cellulose decreases with the alkali cation radius, i.e., LiOH > NaOH > KOH. Our findings are helpful for designing better green solvents for cellulose.Keywords:neutron total scattering;cellulose;dissolution mechanism;layered structure;complexation
研究领域:
化学
·
材料科学
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高影响因子
Food Packaging and Shelf Life
·
2024
·
IF 8.5
Honglian Guo,Yue Xu,Haiyi Chen,Xinyu Si,Menghan Zhou,Enlong Zhu
To extend the shelf-life of cherry tomatoes infected with Botrytis cinerea , this study analyzed the effect of Pichia membranifaciens (PM) on a composite film of gelatin (Gel) and trehalose (Tre), and explored the effect of different concentrations of trehalose on the yeast in the bioactive film and its control effect on cherry tomatoes. Scanning electron microscopy showed that Tre/Gel-based film can be applied as effective carriers to protect the cellular integrity of PM. Fourier-transform infrared spectroscopy, thermogravimetric analysis, and tests of mechanical properties showed that there was interaction between PM and the Tre/Gel base film, which maintained the thermal stability of the base film. The addition of PM reduced the water vapor permeability, hygroscopicity, and moisture content of the Tre/Gel base film, effectively extending the active film's storage time. This study also explored the growth curve of PM and found that 15% trehalose was effective in maintaining PM activity and could result in a 30-day survival rate of 62% for PM in Tre/Gel films, with 72.41% inhibition of B. cinerea . Finally, the PM/Tre/Gel film significantly reduced the incidence of decay in cherry tomatoes and improved the storage quality and fungal resistance of the fruit. Thus, PM/Tre/Gel films may be applied as a new antifungal packaging material for the preservation of fresh fruit.
研究领域:
食品科学
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微生物学
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高影响因子
Applied Materials Today
·
2025
·
IF 6.9
Qianwen Zhang,Weiqing Zheng,Lingqin Cai,Jia Wan,Yongzhi Liang
Conductive hydrogels have garnered extensive attention in flexible sensing technologies due to their unique combination of mechanical compliance and electrical conductivity. However, conventional systems face a critical limitation that the inability to synergistically integrate optical transparency, balanced mechanical/electrical properties, fast self-recovery, and anti-freezing property, thereby constraining their advanced applications. This study explores a facile “one-step” strategy to engineer a poly (acrylic acid)-Zr 4+ /trehalose hydrogel, where biologically derived trehalose serves dual roles as both a cryoprotectant (enabling -45 °C operability) and mechanical toughener. The Zr 4+ -enabled ionic coordination network simultaneously establishes a stiff structured matrix, yielding multifunctionality such as high transparency (up to 92 %), robust mechanical performance (2.86 MPa tensile strength, 1542 % strain, 11.2 MJ/m 3 toughness), high ionic conductivity (32.1 mS/cm), and rapid autonomous recovery (99.6 % efficiency within 10 min). Notably, we developed an innovative ultrafast healing mechanism achieving 79.2 % healing efficiency in just 1 min, overcoming the time-dependent limitations of conventional dynamic networks, ensuring durability under cyclic mechanical stress. This systemic integration of previously incompatible properties positions the hydrogel as a candidate for multifunctional flexible sensors, particularly in extreme environments where traditional systems fail. Its high ionic conductivity and subzero resilience enable reliable sensing in extreme environments, while sensitive strain/pressure detection ensures precise monitoring of subtle motions. The synergy between anti-freezing capability of trehalose and dynamic network of zirconium addresses critical challenges in wearable sensors, combining durability, environmental adaptability, and signal fidelity.
研究领域:
生物材料
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柔性电子
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生物医学工程
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可穿戴传感器
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高分子化学
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力学-电学耦合材料
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高影响因子
危险品化学品经营许可证（带存储）