Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Miao Ting,Basit Abdul,Liu Junquan,Zheng Fengzhen,Rahim Kashif,Lou Huiqiang,Jiang Wei
Xylanases with high specific activity has been focused with great interest as a useful enzyme in biomass utilization. The production of recombinant GH11 xylanase (MYCTH_56237) from Myceliophthora thermophila has been improved through N-terminal signal peptide engineering in P. pastoris. The production of newly recombinant xylanase (termed Mtxyn11C) was improved from 442.53 to 490.7 U/mL, through a replacement of α-factor signal peptide with the native xylanase signal peptide segment (MVSVKAVLLLGAAGTTLA) in P. pastoris. Scaling up of Mtxyn11C production in a 7.5 L fermentor was improved to the maximal production rate of 2503 U/mL. In this study, the degradation efficiency of Mtxyn11C was further examined. Analysis of the hydrolytic mode of action towards the birchwood xylan (BWX) revealed that Mtxyn11C was clearly more effective than commercial xylanase and degrades xylan into xylooligosaccharides (xylobiose, xylotriose, xylotetraose). More importantly, Mtxyn11C in combination with a single multifunctional xylanolytic enzyme, improved the hydrolysis of BWX into single xylose by 40%. Altogether, this study provided strategies for improved production of xylanase together with rapid conversion of xylose from BWX, which provides sustainable, cost-effective and environmental friendly approaches to produce xylose/XOSs for biomass energy or biofuels production.
研究领域:
生物技术
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酶学
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生物化学工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
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IF 8.2
Lixi Cai,Yanqi Gao,Yunmeng Chu,Yuanqing Lin,Lixing Liu,Guangya Zhang
Tailoring magnetic nanocarriers with tunable properties is of great significance for the development of multifunctional candidate materials in numerous fields. Herein, we report a one-pot biomimetic silicification-based method for the synthesis of silica-coated magnetic nanoparticles. The synthesis process was mild, low cost, and highly efficient, which took only about 21 min compared with 4.5–120 h in other literature. Then, the carriers had been characterized by VSM, SEM, TEM , XRD, FT-IR, and EDS to confirm their function. To evaluate the usefulness of the carriers, they were adopted to couple the purification and immobilization of β-1,3-xylanase from the cell lysate in a single step with high immobilization yield (92.8 %) and high activity recovery (82.4 %). The immobilized enzyme also retained 58.4 % of the initial activity after 10 cycles and displayed good storage properties, and improved thermal stability , which would be promising in algae biomass bioconversion as well as other diverse applications.
研究领域:
生物化学
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纳米技术
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酶工程
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2023
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IF 3.3
Wenbin Jin,Kaipeng Wang,Xueyang Xu,Yiling Xu,Fengwei Li,qingzhi ji,Xiaodong Chen,Xiaohong Yu
Ferulic acid is a natural phenolic chemical which widely present in plants and widely used in food and cosmetic industries. It is used as the cross-linking agent for the preparation of edible films, and as skin protection agents. Enzymatic hydrolysis of lignocellulose to produce ferulic acid has been studied for many years and is rarely used in the industry due to easy deactivation of enzymes. Deep eutectic solvent (DES) is exploited as a novel co-solvent for improving the activity and stability of enzyme. A mixed enzyme system composed of feruloyl esterase and xylanase was constructed for catalytic degradation of de-starched wheat bran (DSWB) to produce ferulic acid. When the ratio of feruloyl esterase to xylanase reached 1:1, the release of ferulic acid reached the maximum. Four DES was synthesized and evaluated the effects of compositions and concentrations on the release of ferulic acid from DSWB. The mixed enzymatic system showed the best performance in the ChCl-Gly DES aqueous solution (volume ratio of 1:2). Through the determination of the enzymatic properties and catalytic conditions, the mixed enzymatic system was applied to the catalytic reaction. Ferulic acid yield 4.2 mg/g DSWB was obtained under the optimum conditions. The results suggested that the efficient production of ferulic acid from lignocellulosic biomass can be realized through the synergistic effect of feruloyl esterase and xylanase in DES aqueous solution.
研究领域:
生物技术
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酶学
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化学工程
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材料科学
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DOI
高影响因子
APPLIED SOIL ECOLOGY
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2026
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IF 5.6
Ruijia Luo,Fangyu Xu,Jiajun Wang,Tengyang Gao,Ying Liu,Wansong Zong,Liang Tang,Haoyu Sun,Minghong Wu
Veterinary antibiotic residues are increasingly detected in the soil environment, therefore raising concerns about their effects on soil health. Xylanases are key soil extracellular enzymes responsible for hemicellulose degradation and organic carbon turnover; however, their responses to antibiotic exposure, particularly at the level of direct intermolecular interactions, remain poorly understood. Considering their extensive use and frequent detection in soil media, sulfadiazine (SD) and tetracycline hydrochloride (TC) were chosen as representative veterinary antibiotics for this study. The molecular mechanisms underlying the effects of the SD, TC, and SD + TC mixtures on the enzymatic activity of endo -β-1,4-xylanase (XYL) were investigated using various spectroscopy techniques, interactive effect assessments, and molecular docking studies. The results revealed that SD inhibited XYL activity in a concentration-dependent manner, whereas TC increased XYL activity in a dose-dependent manner. These alterations in XYL activity were mediated by spontaneous antibiotic–enzyme interactions at a 1:1 stoichiometric ratio, rather than by the induction of significant structural perturbations in the enzyme. Furthermore, SD and TC were found to target distinct regulatory sites in XYL, which may explain their opposite effects: SD interacted with the catalytic center, while TC bound to the dimer interface. Molecular modeling further visualized and corroborated the interaction behaviors of the tested antibiotics at their respective binding sites. Moreover, the interactive effect of the SD + TC mixture on the XYL activity shifted from synergistic to antagonistic when the SD-to-TC ratio decreased, indicating that TC binding at the inter-subunit interface might directly modulate the binding behavior of SD. This study establishes a methodological framework for exploring the intermolecular interactions between typical veterinary antibiotics and soil biomacromolecules, thereby providing critical evidence for determining the molecular-scale effects of antibiotic contaminants and facilitating precision risk assessment in the soil environment.
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DOI
高影响因子
BRAZILIAN JOURNAL OF BIOLOGY
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2021
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IF 1.651
Huang Y.,Zhang N.-J.,Zhao Z.
As an important enzyme, xylanase is widely used in the food, pulp, and textile industry. Different applications of xylanase warrant specific conditions including temperature and pH. This study aimed to carry out sodium alginate beads as carrier to immobilize previous reported mutated xylanase from Neocallimastix patriciarum which expressed in E. coli, the activity of immobilization of mutated xylanase was elevated about 4% at pH 6 and 13% at 62 °C. Moreover, the immobilized mutated xylanase retained a greater proportion of its activity than the wide type in thermostability. These properties suggested that the immobilization of mutated xylanase has potential to apply in biobleaching industry.
研究领域:
酶工程
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工业微生物学
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生物技术
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DOI
PubMed
高影响因子
Biocatalysis and Agricultural Biotechnology
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2025
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IF 3.8
Han Lichun,Zhong Xiufang,Fu Zhilei,Yan Jinlong,Xiao Lin,Liu Xiaoyan,Rana Abdul Basit,Wang Jinpeng,Yin Dafeng,Fan Guangsen
Wheat bran, rich in phenolic compounds like ferulic acid (FA), faces limited bioconversion due to its recalcitrant lignocellulosic structure. This study developed a synergistic catalytic system using feruloyl esterase (BpFae03105) and xylanase (XynAS) for efficient conversion into FA and xylo-oligosaccharides (XOS). A multi-stage optimization strategy was employed. First, the effective ranges of eight basic parameters were determined through single-factor experiments. Then, Plackett-Burman design was used to screen for significant factors influencing FA yield, identifying enzymatic hydrolysis temperature, time, and enzyme addition ratio as the most critical. The conditions were further optimized using the Box-Behnken response surface method, resulting in the following optimal conditions: enzymatic hydrolysis temperature of 43 °C, reaction time of 4.2 h, shaker rotational speed of 120 rpm, pH 5.0 phosphate buffer, solid-to-liquid ratio of 1:115 (w/v), enzyme addition ratio (BpFae03105:XynAS) of 1:11.2, BpFae03105 concentration of 8 U/mL, and wheat bran particle size greater than 20 mesh. Under these conditions, the FA yield reached 77.98%, a 2.65-fold increase compared to the unoptimized process. In addition, XOS production achieved a substantial yield of 26.24%, marking a significant breakthrough in co-product generation. The proposed mechanism suggests XynAS degrades xylan, disrupting the lignocellulosic matrix and exposing feruloyl ester groups, thereby enhancing substrate accessibility and BpFae03105 catalytic efficiency. The synergistic enzymatic action overcame limitations of traditional individual enzymes systems, particularly product inhibition, enabling integrated wheat bran valorization. Overall, this study provides a novel, scalable biorefinery approach for high-value utilization of agricultural by-products, offering economic and environmental benefits for sustainable biomass processing.
研究领域:
生物化学
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生物技术
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酶工程
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可再生能源
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农业副产物利用
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产品关联
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2021
·Vol. 193
·期 11
·3469-3482
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IF 2.926
Li Hailong,Chen Xindong,Wang Can,Chen Xuefang,Guo Haijun,Xiong Lian,Zhang Hairong,Huang Chao,Chen Xinde
Understanding factors that affect the catalytic efficiency and synergism of enzymes is helpful to enhance the process of bioconversion. In this study, birch wood (BW) was sequentially treated by delignification (DL), deacetylation (DA), and decrystallization (DC) treatments. The physiochemical structures of treated BW were characterized. Moreover, the influences of sequential treatments on the catalytic efficiency and synergism of xylanase and cellulase were studied. DL treatments efficiently improved the conversion of cellulose and xylan. A high degree of synergy (DS) between xylanase and cellulase was produced during hydrolysis of DL-treated BW. DA treatments enhanced xylan conversion but reduced the DS between xylanase and cellulase for xylan hydrolysis, whereas DC treatments enhanced cellulose conversion but reduced the DS between xylanase and cellulase for cellulose hydrolysis. The cellulose conversion of lithium chloride/N,N-dimethylacetamide (LiCl/DMAc)-treated BW (89.69%) was higher than the cellulose conversion of ball milling (BM)-treated BW (81.63%), whereas the xylan conversion of LiCl/DMAc-treated BW (83.77%) was lower than the xylan conversion of BM-treated BW (87.21%). This study showed that the catalytic efficiency and synergism of xylanase and cellulase are markedly affected by lignin hindrance, hemicellulose acetylation, and cellulose crystallization. Graphical abstract
研究领域:
生物化学
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可再生资源与环境工程
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DOI
高影响因子
Innovative Food Science & Emerging Technologies
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2025
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IF 6.8
Kexun Liu,Zhengjie Xie,Yan Huang,Huayi Suo,Guohua Zhao,Damao Wang
A two-step strategy integrating cellulase/xylanase hydrolysis (pH 4.90, 50.5 °C, 5 h) and Lactobacillus acidophilus 336,636 fermentation (48 h) is developed to enhance the valorization of lignocellulosic brewer's spent grain (BSG). This treatment yielded significant improvements: total phenolic content increased by 2.5-fold ( p < 0.05), flavonoids exhibited a 2.8-fold elevation (p < 0.05), and Ferric-Reducing Antioxidant Power (FRAP) demonstrated a twofold increase compared to untreated BSG. Hydration properties were also enhanced, with water-holding capacity improving by 61 % and soluble protein content rising by 78 %. Metabolomics analysis revealed upregulated pathways for isoquinoline alkaloid biosynthesis and phenylalanine metabolism, alongside the emergence of novel bioactives such as fraxin. In bread-making trials, substituting ≤15 % BSG for wheat flour maintained equivalent texture and volume ( p < 0.05) while elevating dietary fiber content by 2.94-fold ( p < 0.05), protein content by 1.15-fold (p < 0.05), and antioxidant activity by 2.11-fold (p < 0.05). However, exceeding 20 % substitution disrupted gluten networks, leading to reduced volume and sensory acceptability ( p < 0.05). Mechanistically, synergistic release of phenolic acids and microbial β-glucan secretion during fermentation enhanced water-binding capacity and flavor development, enabling this strategy to convert beer waste into nutrient-dense, antioxidant-enriched functional ingredients for staple foods.
研究领域:
食品科学与技术
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生物加工与发酵技术
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废弃物高值化利用
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功能食品
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生物化学
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可持续农业
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Xiaozan Dai,Guanchen Liu,Quan Zhang,Chao Zhu,Huipeng Gao,Youduo Wu,Chi Cheng,Hailong Lin,Chuang Xue
The fungus Trichoderma reesei is widely employed for industrial enzyme production. However, the mechanisms coordinating enzyme production with amino acid biosynthesis and metabolism remain incompletely understood. Here, we characterized a novel C2H2 zinc finger transcription factor, TrTRC-1, in T. reesei. The deletion of TrTRC-1 enhanced cellulase, xylanase, and extracellular protein production by 9.8–32.4% compared to that in T. reesei Rut C30, while simultaneously stimulating mycelial growth and conidiation. Transcriptomic analysis revealed that the deletion of TrTRC-1 significantly altered the expression of genes associated with primary metabolic pathways, amino acid biosynthesis/metabolism, and carbohydrate-active enzymes. In a 5 L bioreactor, T. reesei ΔTrTRC-1 achieved an FPase activity of 17.35 IU/mL and exhibited robust saccharification efficiency on lignocellulosic biomass. These findings demonstrate that TrTRC-1 serves as a multifunctional regulator in T. reesei, advancing our understanding of the regulatory network that balances enzyme production with cellular metabolism and providing a strategic basis for constructing microbial chassis strains with industrial application prospects.
研究领域:
微生物学
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分子生物学
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工业生物技术
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真菌遗传学
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代谢工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Yao Lu,Ying Sun,Yamin Dong,Zhenyong He,Yiwen Xiao,Boliang Gao,Du Zhu
Multifunctional xylanases present considerable potential across various sectors, including the food, chemical and biorefinery industries. Herein, a novel trifunctional xylanase Xyn1.28, identified from the lignocellulose-degrading endophytic fungus Chaetomium globosum DX-THS3, exhibits high xylanase activity (230.37 ± 2.69 U/mg) along with notable endo -glucanase activity and rare arabinofuranosidase activity, which accounted for 17–33 % and 43 % of the xylanase activity, respectively. When combined with commercial cellulase cocktail Celic CTec2 at a 1:10 activity ratio, a significant synergistic effect enhanced sugar release by 27.5 % during unpretreated corn straw degradation. Alkali-treated corn cob degradation efficiency was also improved, reducing the time to achieve maximum sugar yield by 12 h. The sugar release profiles during the initial degradation stages indicated that Xyn1.28 plays a crucial role in the initial stages of hydrolyzing biomass. Furthermore, molecular docking analysis indicated that the unique site GLN-74 may significantly influence the arabinofuranosidase activity of Xyn1.28 by forming a hydrogen bond with HIS-108. Experiments involving the mutated GLN-74 further confirmed the critical role of this site in the arabinofuranosidase activity of Xyn1.28. Overall, this rare multifunctional endo -xylanase is well-positioned to facilitate the development of efficient lignocellulose degradation systems and advance cost-effective biomass conversion.
研究领域:
生物化学
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酶工程
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可再生资源
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产品关联
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
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2020
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IF 3.448
Ting Liu,Baoyou Pei,Jinqing Lin,Guangya Zhang
β-1,3-xylanase plays an important role in the conversion of algal biomass into renewable chemical commodities and functional xylooligosaccharides. Efficient immobilization of the target enzymes that could integrate the separation, purification, and immobilization in one-step may have great potentials. The cheap and easily obtained pitch was adopted as the raw material to prepare the hyper-cross-linked polymers with the specific surface areas of 560 m 2 /g and the Ni 2+ loading about 80 mg/g. As a carrier, it could immobilize and purify the β-1,3-xylanases directly from the crude cell debris with the activity recovery of (80.66 ± 0.87) %. The half-life of the immobilized β-1,3-xylanases was 50 min at 50 °C, which was significantly longer than the free ones with only 14 min. The immobilized β-1,3-xylanases could retain 65 % of the activity after stored at 30 ℃ for 80 h, they could also retain 87 % of the original activity after 15 cycles of reuse. In terms of potential applications in the manipulation of algae Caulerpa lentillifera , the immobilized β-1,3-xylanases could release 30 % more reducing sugar in 85 h. The method proposed here is promising for algal biomass manipulation because of its low cost, stable storage, convenient recycling and excellent reusability.
研究领域:
生物化学
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可再生能源
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酶工程
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2018
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IF 3.129
Yu Zhu,Chenghu He,Haixiao Fan,Zhaoxin Lu,Fengxia Lu,Haizhen Zhao
In order to improve the cholesterol-binding capacity (CBC) of foxtail millet bran dietary fiber (FMBDF), xylanase-catalyzed hydrolysis was used. Single-factor experimental design and orthogonal experimental design were applied to optimize the hydrolysis conditions. The optimal hydrolysis conditions were as following: hydrolysis pH 3.8, xylanase dose 50 U/g FMBDF, hydrolysis temperature 50 °C and hydrolysis time 2 h. Under above conditions, the CBC value (11.57 mg/g) of the xylanase modified FMBDF (X-FMBDF) was 2.23 fold of FMBDF (5.19 mg/g). Analysis of monosaccharide composition, infrared spectrum and scanning electron microscopy showed that xylanase treatment could change the monosaccharide composition and microstructure of FMBDF, which contributed to the binding of dietary fiber to cholesterol. It was suggested that enzymatic treatment was a good method to modify the functional properties of dietary fibers.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）