SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Xiaoxia Liu,Ronghao Liu,Yiming Lu,Qian Sun,Wenfeng Xue,Mengmeng Cheng,Yanzhao Yang
Recycling gold from e-waste is not only beneficial to environmental protection, but also to resource sustainability. However, it remains a great challenge due to the limitations of high cost, lengthy balance process and the interference of coexisting metals. Herein, a family of low-cost, nitrogen-rich content and acid-stable imidazolium-based poly(ionic liquid)s, designated as P(nDVB-mVBIMCl)s, were synthesized for recovery of gold from e-waste. The obtained P(2DVB-8VBIMCl)s achieves outstanding adsorption capacity (820.5 mg·g −1 ) and rapid equilibrium time (15 min) and satisfactory reusability (more than 8 cycles). XPS, EDS and ion chromatography revealed that the excellent adsorption performance was attributed to synergistic effect of ion exchange and chemical reduction. In addition, the P(2DVB-8VBIMCl)s showed excellent selectivity for gold in actual e-waste leaching solution, exhibited satisfactory selectivity coefficient for Fe 3+ (1.46 × 10 4 ) and Ni 2+ (1.12 × 10 4 ), obtaining 23.4 karat high-purity gold after calcination. In addition, the P(2DVB-8VBIMCl)s shows its good economic feasibility. Overall, this work provides a feasible adsorbent, which becomes a promising candidate for industrial gold recovery from e-waste.
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JOURNAL OF MOLECULAR STRUCTURE
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2023
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IF 3.8
Yunkai Sun,Xiaoxia Wu,Meng Cao,Xiaoqiang Xue,Dongfang Jiang,Zhao Liu
In the classical route of synthesizing of avanafil, the last step of the reaction uses EDCI and HOBT as an acid and amine binding agent. HOBT may contain residues of hydrazine, and potential mutagenic impurities containing acylhydrazide structures may be formed. The intermediate product (M6) of avanafil was synthesized by 6 steps using diethyl ethoxylmethylmalonate and S-methyl isothiourea semisulfide as starting materials. The potentially genotoxic impurity E (Imp-E) of avanafil was given by the reaction of M6 and hydrazine sulfate acid amine, yield of 20%. The single crystal of Imp-E was cultivated by solvent volatilization method, and its structure was analyzed by high resolution mass spectrometry (HRMS) and nuclear magnetic resonance spectroscopy (NMR). The Flack parameter of Imp-E was -0.03(3) by X-ray single crystal diffraction analysis, and the absolute configuration of Imp-E was determined as S configuration. The intramolecular / intermolecular hydrogen bonds of Imp-E make the molecules maintain a stable arrangement in space.
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CARBON
·
2023
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IF 10.9
Xuewei Shi,Alamusi Lee,Bo Yang,Lingxiao Gao,Huiming Ning,Kaiyan Huang,Xiaolin Luo,Lidan Zhang,Jiaofei Zhang,Chao Yang,Bin Gu,Ning Hu
Artificial hydrogel electronic skin (E-skin) sensing systems have received considerable attention due to their ability in providing tactile perception. Recent advancements in hydrogel E-skin have allowed for simultaneous sensing of touch position and pressure through construction of sensor arrays. However, the fabrication process is complex, and the sensing resolution is limited by the size and number of array cells. Here, we report a multi-modal sensory E-skin based on a polyvinyl alcohol (PVA) hydrogel embedded with carbon nano-materials, which can recognize touch position and pressure simultaneously without constructing arrays. Combining the advantages of high electron transfer efficiency of 1D nanotube and 2D graphene nanosheet, the 3D hierarchical cross-linked polydopamine (PDA) modified reduced graphene oxide (rGO)/carboxylic multiwalled carbon nanotube (MWCNT-COOH) composites were proposed as a conductive filler, which was added by nano-doping to hydrogel substrate to gain an elastic modulus and stretchability of the hydrogel similar to that of human skin. Meanwhile, a surface-capacitive touch (SCT) system and piezoresistive technology were adopted to sense touch position and pressure due to the stability of conductive networks and the piezoresistive properties of carbon nanomaterials. Our E-skin device demonstrates superior sensing performance and provides a new direction for designing novel E-skin sensor devices.
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FOOD AND CHEMICAL TOXICOLOGY
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2023
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IF 4.3
Kaiyuan Chen,Yahui Tang,Linhua Lan,Mengxuan Li,Zhongqiu Lu
Acute kidney injury (AKI) induced by diquat (DQ) progresses rapidly, leading to high mortality, and there is no specific antidote for this chemical. Our limited knowledge of the pathogenic toxicological mechanisms of DQ has hindered the development of treatments against DQ poisoning. Pyroptosis is a form of programmed cell death and was recently identified as a novel molecular mechanism of drug-induced AKI. To explore the role of pyroptosis in HK-2 cells exposed to DQ, the plasma membrane damage of the cells was detected by LDH release assay. Western blot was performed to detect the cleavage of GSDME. Proteomics analysis was performed to explore the mechanism of DQ induced nephrotoxicity . FerroOrange probe was used to measure the intracellular Fe 2+ levels. Herein, we show that DQ induces pyroptosis in HK-2 cells. Mechanistically, DQ induces the accumulation of mitochondrial ROS and initiates the cleavage of gasdermin E (GSDME) in an intrinsic mitochondrial pathway. Knockout of GSDME attenuated DQ-induced cell death. Further analysis revealed that loss of FTH1 induces Fe 2+ accumulation, contributing to DQ-induced pyroptosis. Knockdown LC3B could help restore the expression of FTH1 and improve cell viability . Moreover, we found DFO , an iron chelator , could reduce cellular Fe 2+ levels and inhibit pyroptosis. Collectively, these findings suggest an unrecognized mechanism for GSDME-dependent pyroptosis in DQ-induced AKI.
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ACS Catalysis
·
2023
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IF 12.9
Zhen Chen,Haixin Chen,Kun Wang,Jian Chen,Ming Li,Yi Wang,Panagiotis Tsiakaras,Shuqin Song
The photocatalytic oxygen reduction reaction (ORR) is a promising strategy that generates H2O2 with a lower energy input. Although great progress has been made in the development of photocatalysts, poor accessibility of O2 in photocatalysts interfacial microenvironment is the rate-limiting factor for the photocatalytic 2e– ORR and still little explored. Here, we design a simple method to regulate the hydrophobicity and O2 accessibility at a controllable crystal facet ratio of TiO2 photocatalyst by a facile stearic acid (SA) modification. The adsorption structure of SA bonding with TiO2 has been investigated in detail by Raman spectra, X-ray photoelectron spectroscopy, Fourier transform infrared spectra, and density functional theory calculations. It has been found that the stable ester bond is formed between SA molecules and the TiO2 surface, thus leading to the enhanced hydrophobicity with the increase of SA loading. The highest photocatalytic H2O2 production rate from 2e− ORR on SA-modified TiO2 can reach up to 3160 μM h–1 g–1, which is 1.69 times that of the corresponding pure TiO2 sample. From the results of electrochemical impedance spectroscopy and molecular dynamics simulation, the increase of the O2 concentration in the interfacial microenvironment benefiting from the SA modification is responsible for the enhancement of 2e– ORR activity rather than the increase of hydrophobicity.
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RSC Advances
·
2023
·Vol. 13
·期 22
·15063-15076
·
IF 3.9
Song Wang,Yonggang Zhang
Advanced oxidation processes are frequently applied to a variety of refractory organic wastewater, but rarely is electro-Fenton combined with activated persulfate technology applied to the removal of refractory pollutants. In this work, the electro-Fenton process was combined with zero-valent iron (ZVI) activated peroxymonosulfate (PMS), two advanced oxidation processes based on different radicals, to form the ZVI-E-Fenton-PMS process to treat wastewater, whose main advantages are the generation of more reactive oxygen species and the reduction of oxidant cost to achieve rapid removal of pollutants. This process can not only produce H2O2 and activate PMS at the cathode, but also reduce Fe(III) to realize the sustainable Fe(III)/Fe(II) redox cycle. The main reactive oxygen species in the ZVI-E-Fenton-PMS process were found to be ˙OH, SO4˙− and 1O2 by radical scavenging experiments and electron paramagnetic resonance (EPR), and the relative contributions of the three reactive oxygen species for the degradation of MB were estimated to 30.77%, 39.62% and 15.38%, respectively. Then, by calculating the ratio of the relative contributions of each component to the removal of pollutants at different PMS doses, it was found that the synergistic effect of the process was better when the proportion of ˙OH in the oxidation of reactive oxygen species (ROS) was higher and the proportion of non-ROS oxidation increased year-on-year. This study provides a new perspective on the combination of different advanced oxidation processes and reveals the advantages and potential of this process for application.
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Cell Communication and Signaling
·
2023
·Vol. 21
·期 1
·1-17
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IF 8.4
Zhao Weiming,Wang Lan,Yang Juntang,Chen Xinyu,Guo Xiaoshu,Xu Kai,Wang Ningdan,Zhao Wenyu,Xia Cong,Lian Hui,Rosas Ivan,Yu Guoying
Background Matrix metalloproteinases (MMPs) play important roles in remodeling the extracellular matrix and in the pathogenesis of idiopathic pulmonary fibrosis (IPF). MMP19, which is an MMP, was significantly upregulated in hyperplastic alveolar epithelial cells in IPF lung tissues and promoted epithelial-mesenchymal transition (EMT). Recent studies have demonstrated that endothelial-to-mesenchymal transition (E(nd)MT) contributes to pulmonary fibrosis. However, the role of MMP19 in pulmonary vascular injury and repair and E(nd)MT remains unclear. Methods To determine the role of MMP19 in E(nd)MT and pulmonary fibrosis. MMP19 expressions were determined in the lung endothelial cells of IPF patients and bleomycin (BLM)-induced mice. The roles of MMP19 in E(nd)MT and endothelial barrier permeability were studied in the MMP19 cDNA-transfected primary human pulmonary microvascular endothelial cells (HPMECs) and MMP19 adenoassociated virus (MMP19-AAV)-infected mice. The regulatory mechanism of MMP19 in pulmonary fibrosis was elucidated by blocking its interacting proteins SDF1 and ET1 with AMD3100 and Bosentan, respectively. Results In this study, we found that MMP19 expression was significantly increased in the lung endothelial cells of IPF patients and BLM-induced mice compared to the control groups. MMP19 promoted E(nd)MT and the migration and permeability of HPMECs in vitro, stimulated monocyte infiltration into the alveolus, and aggravated BLM-induced pulmonary fibrosis in vivo. SDF1 and Endothelin-1 (ET1) were physically associated with MMP19 in HPMECs and colocalized with MMP19 in endothelial cells in IPF patient lung tissues. AMD3100 and bosentan alleviated the fibrosis induced by MMP19 in the BLM mouse model. Conclusion MMP19 promoted E(nd)MT by interacting with ET1 and stimulated monocyte infiltration into lung tissues via the SDF1/CXCR4 axis, thus aggravating BLM-induced pulmonary fibrosis. Vascular integrity regulated by MMP19 could be a promising therapeutic target for suppressing pulmonary fibrosis.
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MOLECULAR PHARMACEUTICS
·
2019
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IF 4.396
Xiao-Ling Xu,Meng-Lu Zhu,Di Liu,Gao-Feng Shu,Jing Qi,Yan Lu,Fei Wang,Xiao-Ying Ying,Jian Chen,Yong-Zhong Du
Therapeutic goals for metastatic breast cancer, including shrinkage of established metastasis and suppression of movement of tumor cells, are often hard to achieve and remain the main obstacles restricting the antimetastatic efficacy of targeted drug delivery systems (TDDSs). Herein, we proposed an E-selectin-targeting nanoplatform for the systemic treatment of metastatic breast cancer. Versatile functions, including killing the circulating tumor cells, shrinking the established lesions, as well as inhibiting the movement of tumor cells, were integrated into doxorubicin-loaded sialic acid-dextran-octadecanoic acid (SDO) micelles (SDD). The prepared SDD micelles could not only inhibit lung and liver metastasis in the orthotopic 4T1 tumors model, but also decrease the metastatic lesions in the metastatic 4T1 cell model, resulting in 27.33% reduced number of metastatic nodules when compared to those without sialic acid modification. It was found that the good antimetastatic effect of SDD was only partially attributed to its ability on removing metastatic cells and metastases. Most importantly, the blank SDO micelles left in the lesion could further inhibit the cell migration and cell–cell binding. These results suggest that SA-driven TDDS has the potential for specific targeting and effective treatment of cancer metastasis.
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TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY
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2019
·
IF 0.971
Yu Wang,Jiayi Li,Rui Zhou,Lincai Yang,Peisheng He,Lixuan Chen,Liying Luo,Haowen Chen,Lian Wei
The protective effects of vitamin E (45, 150, and 500 mg/kg/d) against reproductive toxicity induced in male Kunming mice by intragastric administration of 120 mg/kg/d bisphenol A was investigated. After two weeks, relative organ weights, sperm counts, sperm abnormality rates and serum testosterone concentrations were determined. Epididymal sperm counts were significantly decreased, sperm abnormality was increased, and serum testosterone concentrations were dramatically reduced following bisphenol A exposure. Vitamin E treatment led to a marked increase in sperm numbers and a decrease in the proportion of malformed sperm in a dose-related manner; serum testosterone levels were also restored. This suggests that vitamin E could be used as prophylactic agent for preventing bisphenol A-induced reproductive toxicity.
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EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2018
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IF 3.466
Wen-Bin Zhao,Chi-Xiao Qiu,Ying Shen,Wen-Hui Liu,Jie Zhou,Ying-Chun Xu,Zhan Zhou,Shu-Qing Chen
Antibody-drug conjugates (ADCs) consist of cytotoxic agents covalently conjugated to monoclonal antibodies that substantially improve antitumour activity and reduce systemic toxicity. With the growing number of ADCs in clinical applications, more accurate bioanalysis data are urgently needed to facilitate the development and rational use of ADCs. Herein, we used antigen-positive cells as antigen carriers and ofatumumab (OFA-HL) and ofatumumab-based ADC (OFA-HL-MMAE) as examples to establish a new ligand-binding assay (LBA) method based on flow cytometry. We proved that the new method met the required analytical performance criteria and the lower limit of quantitation (LOQ) was 0.2 μg/mL. In addition, the LOQ of the quantitative OFA-HL flow cytometry method was reduced to 0.025 μg/mL by choosing an optimized fluorescent antibody, which indicated that the LOQ of the new method can be improved. What's more, the new method showed good stability and specificity when we used it to determine the concentrations of OFA-HL and OFA-HL-MMAE in mouse serum. During the bioanalysis of ADCs, various factors should be considered. Therefore, choosing optimal methods for ADC bioanalysis is necessary. This new method using in situ antigens not only extends the scope of application of the conventional LBA methods by avoiding the need for soluble antigens, but also improves the authenticity of ADC bioanalysis as a supplementary approach, which is valuable for developing accurate ADC assays.
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FOOD HYDROCOLLOIDS
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2019
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IF 5.839
Hongqiang Chen,Like Mao,Zhanqun Hou,Fang Yuan,Yanxiang Gao
The role of the oil droplets in emulsion gelation (as active or inactive fillers) was modified by additional emulsifiers, and structures and functionalities of emulsion gels were evaluated. Emulsion gels based on denatured protein (whey protein isolate) were prepared via an acid-induced approach, and four emulsifiers, including glycerol monostearate (MG), tween 20 (TW), modified starch (OSA) and gum Arabic (GA) were tested. The including of MG or TW led to delayed onset of gelation, and the emulsion gels formed were of lower gel strength (described as storage modulus and fracture force), compared with the emulsion gels without additional emulsifiers. Increase in the content of MG or TW resulted in the gels with lower water holding capacity , and slight changes in swelling ratio. The presence of OSA or GA allowed faster gelation of the emulsions, forming gels with higher storage modulus and fracture force. Increase in the content of OSA or GA did not affect WHC of the gels, but contributed to higher swelling ratios. Microstructural observation indicated that the emulsion gels with OSA or GA had smaller pore size. When vitamin E was incorporated within the gels, the emulsifiers presented different effects on its degradation kinetics. Vitamin E degraded much faster in the systems with higher content of MG or TW, and lower retention ratios were obtained after the storage (55 °C for 12 days). On the contrary, the emulsion gels with OSA or GA provided good protection for vitamin E, and increase in the content of the emulsifiers allowed higher retention ratios.
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ECS Journal of Solid State Science and Technology
·
2024
·Vol. 13
·期 3
·037005
·
IF 1.8
Ning Lu,Boyu Guo,Zhikang Rao,Juntao Chen,Ying Xu
Electrochemical impedance sensors are widely used for bacterial detection and interpreted by the equivalent circuit model (ECM) to evaluate the concentration changes on the electrode interface. However, this method is mainly based on prior models and focuses on frequency domain analysis which cannot precisely describe unknown dynamic processes and a fractional surface coverage of electrode. Distribution of Relaxation Times (DRT) method, as an alternative to interpret impedance spectra can be applied to solve the problem. In this research, two electrode schemes with different modification steps were designed to collect impedance data. An Ensemble Tree-SHAP model was then adopted to quantitatively analyze the impedance data processed by ECM-DRT method in time-frequency domain and applied for the detection of Escherichia coli (E.coli). The results showed that using a dataset integrated with time-frequency domain features significantly improved the accuracy of bacterial concentration prediction. As a result, the RMSE of untrained concentration with LightGBM model was 6.4\times 1{0}^{-4}\,log CFU ml−1, with the sensor detection limit about 2.69 log CFU ml−1. The research provided a tool combining time-frequency analysis for accurate prediction of bacterial concentration, which is crucial for evaluation of the antibacterial effect of long-term antibiotics on E.coli in future.
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