Biomolecules
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2024
·Vol. 14
·期 12
·1553
·
IF 4.8
Zifu Ni,Linshang Zhang,Azhen Nie,Huan Wang,Xiaoling Wu
As the only naturally occurring β-amino acid, β-alanine has important application prospects in many fields. Driven by the huge demand, biosynthesis is becoming more and more popular as a potential alternative to the chemical synthesis of β-alanine. Although the direct pathway from L-aspartic acid to β-alanine, catalyzed by L-aspartic acid-α-decarboxylase (PanD), is ideal for β-alanine synthesis, it is hindered by the high cost of the substrate and limited economic viability. In this work, a cell-free dual enzyme cascade system based on methylaspartate lyase (EcMAL) and panD was constructed to safely and efficiently synthesize β-alanine using fumarate as a substrate. Taking the previously engineered EcMAL as the target, CgPanD was finally screened as the best candidate through gene mining, sequence alignment, and enzyme property analysis. Finally, under the optimal conditions of 35 °C, pH 8.0, and EcMAL: CgPanD concentration ratio of 1:5, the yield of β-alanine reached 80% theoretical yield within 120 min. This study provides a potential strategy for the biosynthesis of β-alanine, paving the way for future industrial-scale production.
研究领域:
生物化学
·
合成生物学
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产品关联
DOI
PubMed
高影响因子
Materials Research Letters
·
2024
·
IF 8.6
Xiaoqi Mao,Lianwen Wang,Guodong Sun,Xuezhe Zhang,Yongqing Fu,Longlong Dong
Breaking trade-off barriers of strength-ductility in Ti alloys and their composites has been a key challenge for their engineering applications. Herein, we developed a new strategy of introducing β-Ti ductile zones in heterostructured (Cu@CNTs/TC4)+TC18 composites, which were synthesized using two-stepped ball milling, spark plasma sintering and hot rolling processes. Such composites are composed of TC18-coarse grain zones (CGZ) and (TiC+α'')-reinforced TC4-fine grain zones (FGZ). They achieve an exceptional high strength and high ductility, which are superior to those of TC4 alloys and homogeneous Cu@CNTs/TC4 composites. Heterogeneous interfacial microstructures of TC4-FGZ/TC18-CGZ play an important role in strengthening and toughening effects. The grain-coordinated deformation between TC18-CG and TC4-FG is also the key reason for achieving an exceptional ductility.
研究领域:
材料科学
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力学工程
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产品关联
DOI
高影响因子
Small Methods
·
2024
·
IF 10.7
Lirong He,Xingang Liu,Cheng Han,Dehui Wang,Qi Wang,Xu Deng,Chuhong Zhang
For polyvinylidene fluoride (PVDF) based piezoelectric composites, epitaxial growth of ZnO nanorods (ZnO-nr) piezoceramic layer on PVDF is an effective way to improve their piezoelectric performance. However, the crystal nucleus of ZnO featuring polar surfaces that cannot be directly attached to hydrophobic PVDF with low surface energy. Herein, direct ink writing (DIW) 3D printing is employed for the first time to create β-PVDF reservoirs with significantly enhanced surface energy, facilitating the attachment and epitaxial growth of ZnO-nr. The printed β -PVDF reservoirs designed with programmed macro-pores and abundant inner micropores, enable a higher loading of ZnO-nr by more than one magnitude, thereby boosting the electro-mechanical response. The resulting PVDF/ZnO core–shell piezoelectric energy harvester (PEH) delivers an output voltage of 33.2 V, as well as an unprecedentedly high relative output voltage of 2.76 V/wt.%, which is 2.63 times that of the state-of-the-art 3D-printed PVDF/piezoceramics PEHs. Furthermore, it can differentiate subtle human motions whereas hybrid PEHs cannot distinct. This work demonstrates that the DIW 3D printing approach offers a simple and convenient design idea for creating high performance PEHs.
研究领域:
材料科学
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能源技术
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纳米科技
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Yidong Ming,Yongqi Zhu,Yuan Chen,Bowen Jin,Chenhui Duan,Zihui Liang,Li Zhao,Shimin Wang,Binghai Dong,Haijin Li,Congcong Wu
SnO2 films as a promising electron transport layer (ETL) have been widely used in planar-type perovskite solar cells to achieve an impressive improvement in the conversion efficiency. However, compared with a mesoporous ETL, the interfacial charge carrier transfer of the SnO2 ETL is severely limited due to the issues of oxygen vacancy defects and crystal lattice mismatch between SnO2 and the perovskite, which generally leads to the growth of randomly stacked and porous perovskite layers and subsequently impacts the charge transport and transfer properties. In this work, we developed a facile approach by inducing a bifunctional molecule, β-alanine, into the SnO2 ETL, which can serve as a bridge to modulate the interfacial charge transfer and the perovskite crystallization kinetics. Benefited by the interfacial β-alanine, we grew a highly orientational perovskite layer that exhibited superior charge transport properties. Meanwhile, the β-alanine caused an intimate connection between the perovskite and SnO2 to enhance the interfacial charge transfer. As a result, the power conversion efficiency (PCE) of the β-alanine-modified device achieved a much-improved value of 19.67% and showed high reproducibility. This work provides a way for developing a high-performance ETL toward the scalable fabrication of highly efficient PSCs.
研究领域:
材料科学
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能源科学
✓ 已验证
DOI
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Jie Zhang,Xu Liu,Yu Sun,Zhaoyan Ge,Jian Shen,Jiang Yuan
Chronic diabetic wounds suffer from severe complications caused by long-term high levels of oxidative stress and bacterial infection. Quercetin (Que) has excellent anti-inflammatory, antioxidant, and antibacterial activity, making it a promising drug to address the above issues. To exploit the benefits of Que in a more effective and sustained way to treat diabetic wounds, carboxymethyl β-cyclodextrin (CMCD) was synthesized and conjugated to keratin, then complexed with Que to form Que@Ker-CMCD inclusion, followed by electrospinning with polyurethane (PU) to afford Que@Ker-CMCD/PU mats. The approach significantly enhanced water solubility, bioavailability, and sustained release of Que. Crucially, these mats exhibited robust antioxidant and antibacterial activities. Moreover, the mats fostered an environment conducive to cell proliferation, migration, angiogenesis, and re-epithelialization, pivotal processes in wound healing and remodeling. Consequently, a marked acceleration in remodeling chronic diabetic wounds was observed. In conclusion, this study introduces a novel therapeutic strategy that not only harnesses the multifaceted benefits of Que but also enhances its delivery and performance, offering a promising avenue for the effective treatment of chronic diabetic wounds.
研究领域:
生物材料学
·
药剂学
·
伤口愈合研究
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xian Qu,Yahui Ji,Jieyi Long,Donglin Zheng,Zhonghui Qiao,Yue Lin,Chang Lu,Yifa Zhou,Hairong Cheng
The β-1,6-glucans from Lentinus edodes have a variety of biological activities. However, the research on extraction and separation of β-1,6-glucans from L. edodes is limited and the yield is low. In the present study, we obtained the high-yield and -purity β-1,6-glucans (ALEPA) from L. edodes by using a sequential extraction and separation process, which is simple and suitable for industrialization . ALEPA significantly promoted the proliferation of splenic T lymphocytes and enhanced the phagocytosis activity of peritoneal macrophages in vivo . 16S rRNA sequencing results showed that ALEPA significantly increased the α diversity of gut microbiota and upregulated the relative abundances of short chain fatty acids (SCFAs)-producing bacterial species. Consistently, the SCFAs in the cecum of mice were upregulated. On a mechanical level, we found that the immunomodulatory effect of ALEPA depended on gut microbiota. Collectively, ALEPA is a promising functional food ingredient that regulates gut microbiota and enhances immune function.
研究领域:
食品科学
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免疫学
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微生物学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE RESEARCH
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2012
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IF 2.332
Hai-Min Shen,Hong-Bing Ji
Inspired by β-CD, a macrocyclic oligomers of d -(+)-glucopyranose and a renewable material, which could be obtained from starch, that can promote a lot of organic reactions in water, a green solvent, several amino alcohol-modified β-CDs CD-1 to CD-7 were synthesized in the yields of 36–61%. Their conformations in vacuum and in aqueous solution were optimized by quantum calculation. Their complexes with sodium molybdate prepared in situ were characterized by 1 H NMR and were applied in the asymmetric oxidation of thioanisole. Their performance in inducing enantioselectivity was investigated in detail. For the optimal one, CD-1, moderate enantioselectivity (56% ee) was achieved in aqueous CH 3 COONa–HCl buffer solution (pH 7.0). The abilities of CD-1 to CD-7 to induce asymmetry are highly dependent on the pH value of the reaction medium and the structure of the modifying group. The origin of the moderate enantioselectivity and the reaction mechanism were investigated with the aid of 1 H ROESY NMR studies and quantum calculation. The moderate enantioselectivity was attributed to the two different binding models between CD-1 and thioanisole, which could be defined as intramolecular catalysis and intermolecular catalysis, in which intramolecular catalysis gave ( S )-methyl phenyl sulfoxide and intermolecular catalysis gave ( R , S )-methyl phenyl sulfoxide.
研究领域:
有机化学
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绿色化学
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催化化学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CATALYSIS
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2015
·
IF 1.964
Wenchao Ren,Zile Hua,Tongguang Ge,Xiaoxia Zhou,Lisong Chen,Yan Zhu,Jianlin Shi
Hierarchically structured Ti-β zeolites were prepared using a two-step post-synthesis approach. The physicochemical properties of the resultant Ti- m β-S x materials were determined using various techniques, including X-ray diffraction, scanning electron microscopy, inductively coupled plasma atomic emission spectroscopy, transmission electron microscopy, and ultraviolet-visible and ultraviolet-Raman spectroscopies. The porous structures of the synthesized hierarchical Ti-β zeolites were well preserved. Their catalytic performance was compared with that of pure microporous Ti-β materials using alkene epoxidation as a model reaction. It was found that although comparable catalytic activities were obtained for both types of catalyst in the epoxidation of small molecules, e.g., cyclohexene, the synthesized hierarchical Ti-β gave an enhanced catalytic performance in the epoxidation of bulky 1-dodecene.
研究领域:
材料科学
·
催化化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
·
2015
·Vol. 53
·期 11
·1387-1395
·
IF 3.113
Huan Yu,Jian Sun,Yunti Zhang,Guangyan Zhang,Yanfeng Chu,Renxi Zhuo,Xulin Jiang
A novel kind of graft polymer poly(aspartic acid)-ethanediamine- g -adamantane/methyloxy polyethylene glycol (Pasp-EDA- g -Ad/mPEG) was designed and synthesized for drug delivery in this study. The chemical structure of the prepared polymer was confirmed by proton NMR. The obtained polymer can self-assemble into micelles which were stable under a physiological environment and displayed pH- and β-cyclodextrin (β-CD)-responsive behaviors because of the acid-labile benzoic imine linkage and hydrophobic adamantine groups in the side chains of the polymer. The doxorubicin (Dox)-loaded micelles showed a slow release under physiological conditions and a rapid release after exposure to weakly acidic or β-CD environment. The in vitro cytotoxicity results suggested that the polymer was good at biocompatibility and could remain Dox biologically active. Hence, the Pasp-EDA- g -Ad/mPEG micelles may be applied as promising controlled drug delivery system for hydrophobic antitumor drugs. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2015 , 53 , 1387–1395
研究领域:
生物材料学
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药剂学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
APOPTOSIS
·
2025
·
IF 8.1
Miao Jintian,Jiang Xinyan,Wang Siyun
Cisplatin resistance significantly hinders the efficacy of ovarian cancer treatment, presenting a major challenge in improving patient outcomes. This study identifies the m6A reader protein YTHDF1 as a key regulator of cisplatin resistance in ovarian cancer through its modulation of the FZD7/Wnt/β-catenin signaling pathway. Using cisplatin-resistant ovarian cancer cell lines (A2780/DDP and SKOV3/DDP), we observed elevated YTHDF1 expression, which positively correlated with tumor cell proliferation and migration. Silencing YTHDF1 reduced FZD7 expression, inhibited Wnt/β-catenin signaling, and restored cisplatin sensitivity both in vitro and in vivo. Mechanistic investigations revealed that YTHDF1 binds to m6A-modified FZD7 mRNA, enhancing its stability and translation. Functional studies in xenograft mouse models demonstrated that targeting YTHDF1 suppressed tumor growth and enhanced apoptosis in cisplatin-resistant ovarian cancer cells. These findings highlight the YTHDF1-FZD7 axis as a novel therapeutic target for overcoming cisplatin resistance, paving the way for improved treatment strategies in ovarian cancer.
研究领域:
肿瘤学
·
分子生物学
·
表观遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Licheng Zhang,Jinfeng Li,Lianmin Ji,Lijuan Li
Deep eutectic solvent (DES) is gaining much attention as a sustainable and green solvent for chemical separation. This work proposed a series of β-diketone based DES composed by benzoyl trifluoroacetone as hydrogen donor and compounds with different functional group as hydrogen acceptors. The newly prepared DES presented much higher lithium capacity than the conventional solvent extraction system. Due to the strong electron donating nature of phosphine oxide and hydrophobicity of longer carbon chains, TRPO and HBTA with molar ratio of 1/1 showed the best performance for lithium extraction efficiency and separation effect over sodium. The single crystal of the complex formed by lithium and diketone was obtained for the first time, and the stoichiometry of complex formed by HBTA/TRPO-DES and alkali metal was determined to be 1:1:1 by single crystal X-ray diffraction. The synthesized HBTA/TRPO-DES was used to recover lithium from mother liquor of lithium carbonate which was a complex solution with high molar ratio of Na/Li, the extraction of lithium at O/A = 1/3 could reach 90%, and the separation factor of Li/Na was 335 under that condition. After scrubbing and stripping, the DES could be used to next extraction cycle, and the extraction ability remained practically constant. The present work may provide a novel way for lithium recovery from alkaline solutions.
研究领域:
化学工程
·
环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2022
·
IF 10.53
Xin Qiu,Jian Ke,Wenbei Chen,Huixian Liu,Xiaoping Bai,Yibing Ji,Jianqiu Chen
With the development of pharmaceutical field, the demand for single enantiomer drugs is gradually increasing. Recently, chiral membrane separation technology has great potential in the field of enantioseparation due to its simple preparation, continuous operation and easy scale-up. In this study, a novel β-cyclodextrin-ionic liquid thin film composite membrane (β-CD-IL TFCM) was fabricated by interfacial polymerization method to achieve the enantioseparation of chiral drugs. Various fabrication conditions of the membrane were optimized with tryptophan as a model molecule to improve its enantioselectivity. Under optimal fabrication conditions, the percent enantiomeric excess (e.e.%) of tryptophan was 100.0% and the solute flux (J S ) was 8.0 nmol cm −2 h −1 . Enantioseparation of various chiral drugs was achieved using β-CD-IL TFCM, including propranolol (e.e.% = 73.4%), warfarin (e.e.% = 11.4%), metoprolol (e.e.% = 3.4%), ibuprofen (e.e.% = 17.8%) and terbutaline (e.e.% = 3.3%). In addition, molecular docking simulation was applied to investigate the separation mechanism of chiral drugs, demonstrating that multiple interactions promote the separation of drug enantiomers. These results reveal the excellent enantioseparation performance of β-CD-IL TFCM, which has a wide prospect in realizing industrial application of chiral membranes.
研究领域:
药学
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材料科学
·
分子生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）