JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 43
·期 3
·622-630
·
IF 2.516
Chunying Li,Min Sun,Xiangping Ji,Sen Han,Jiaqing Feng,Wenjuan Guo,Juanjuan Feng
Triazine-based organic polymers@SiO 2 nanospheres were prepared and applied as an extraction coating onto stainless steel wires and the wires were filled into polyetheretherketone tube for in-tube solid-phase microextraction. Taking polycyclic aromatic hydrocarbons as targets, main factors affecting extraction performance of the tube were investigated through coupling to high performance liquid chromatography. Under the optimum conditions, an online analytical method for polycyclic aromatic hydrocarbons was established with large linear ranges (0.010-20 µg/L), low limits of detection (0.003-0.010 µg/L), high enrichment factors (533-2954), and good repeatability (relative standard deviations <1.7% for intraday test, <5.0% for interday test). The analysis method was successfully applied to the detection of trace targets in real water samples and the relative recoveries ranged from 82.9 to 119.9%, which demonstrated the applicability of extraction tube in sample preparation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 3.371
Xiaoqiang Jia,Yun He,Dawei Jiang,Chang Liu,Wenyu Lu
Polycyclic aromatic hydrocarbons (PAHs) like phenanthrene are harmful to human health and are difficult to eliminate from environments. In this study, a phenanthrene degrading strain, Pseudomonas aeruginosa PH1, was isolated from oil-contaminated soil. An engineered co-culture consortium was subsequently constructed comprising two engineered bacterial strains, Escherichia coli HY1 and P. aeruginosa PH2, for use in phenanthrene bioremoval. E. coli HY1 harbored two modules of terminal dioxygenases and an electron transfer chain, while P. aeruginosa PH2 contained a module of catechol 1,2-dioxygenase. Phenanthrene removal rates by the wild-type degrader, P. aeruginosa PH1, were 45%, 43%, and 81%, in mineral salt medium supplemented with 100 mg/L phenanthrene, 100 mg/L phenanthrene and 2% (wt/vol) oil, and 100 mg/L phenanthrene and 2% (vol/vol) n -hexadecane, respectively. In contrast, the engineered co-culture consortium removed 71%, 80%, and 88% of the phenanthrene in the corresponding treatments, thus achieving much higher removal efficiencies. Moreover, gas chromatography-mass spectrometry confirmed that the engineered co-culture consortium first oxidized phenanthrene to 9,10-dihydroxy phenanthrene and 1,2-dihydroxy phenanthrene and then metabolized these intermediates via catechol to carbon dioxide and water. During this process, E. coli HY1 dominated the initial oxidation steps, while P. aeruginosa PH2 was primarily responsible for ring cleavage. These results indicate that the engineered co-culture consortium described here represents a potential alternative and attractive means to remove PAHs from natural environments.
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Science
·
2018
·Vol. 6
·期 8
·2172-2188
·
IF 5.831
Haifeng Sun,Dinglingge Cao,Yanhong Liu,Hui Wang,Xue Ke,Tianyuan Ci
Tumor metastasis has become a major obstacle for the clinical treatment of malignant breast cancer. Thus, a delivery system capable of both antitumor and anti-metastasis efficacy is desired. In this work, a low molecular weight heparin (LMWH)-based reduction-sensitive delivery system was developed, aiming to combine the properties of both the antitumor drug and active excipients to achieve a synergistic antitumor and anti-metastatic efficiency as well as resolving the potential adverse effects of both doxorubicin and LMWH. That is, the efficiency of DOX could be enhanced and its toxicity could be lowered. Meanwhile, the biological properties of LMWH could be strengthened and its bleeding risk could also be alleviated. Briefly, drug-loaded crosslinked nanoparticles (DOX/cLLHC2) had a longer blood circulation time and exhibited a rapid reduction-triggered release of DOX. The in vitro assays revealed that DOX/cLLHC2 exhibited a higher cellular uptake and cytotoxicity compared to free DOX. Meanwhile, for the existence of LMWH, DOX/cLLHC2 inhibited cell migration and invasion, as well as effectively inhibited the tube-like formation of human umbilical vein endothelial cells. DOX/cLLHC2 possessed a superior tumor accumulation compared to lipoic acid unmodified nanoparticles and free DOX. Also, DOX/cLLHC2 exhibited encouraging antitumor and anti-metastasis efficiency in an orthotopic breast cancer model. Overall, DOX/cLLHC2 could exert antitumor, anti-metastasis, and anti-angiogenesis efficacy simultaneously as well as having lower systemic toxicity, which makes it suitable for the therapy of metastatic breast carcinoma.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY AND ECOLOGY
·
2018
·
IF 1.091
Tingfeng Liu,Keqiang Ding,Guang Guo,Feng Yang,Lihong Wang
Effect of hydroxypropyl-β-cyclodextrin (HPCD) on the bioavailability and biodegradation of the polycyclic aromatic hydrocarbons (PAHs) pyrene (PYR) and benzo[α]pyrene (BaP) in spiked soils was investigated in 14-week incubation experiments. To evaluate the effect of HPCD in soils with a different matrix, humic substance (HS) was added into soil samples. A 6-h Tenax TA extraction method was used to evaluate pollutants bioavailability. The biodegraded and extracted fractions were compared to evaluate the impact of HPCD on PAHs biodegradation. Results indicated positive effects of HPCD on fast desorption behaviours of PAHs. The biodegraded fraction was consistent with that of the extracted for PYR. However, in terms of BaP, the results were contrary which suggests that biological factors may be limiting factors for BaP pollution remediation. HS weakened the HPCD solubilisation effect while accelerated the decay of PYR and BaP, also implying that bioavailability was not the sole factor limiting PAH biodegradation. In addition, analysis of microbial communities demonstrated that HPCD inhibited the growth of some soil bacteria while HS promoted the evolution of some soil microorganisms. A limited population of hydrocarbon degrader populations led to observing incomplete PAH biodegradation even in the presence of HPCD.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Xingru Hu,Chaohai Wang,Jiansheng Li,Rui Luo,Chao Liu,Xiuyun Sun,Jinyou Shen,Weiqing Han,Lianjun Wang
Developing novel coating materials for fast and sensitive solid-phase microextraction (SPME) is highly desired but few are achieved. In this work, a new material of metal–organic framework (MOF)-derived hollow carbon nanocubes (HCNCs) was prepared as a fiber coating material for SPME. The HCNC-coated fiber (denoted as HCNCs-F) exhibited a better enrichment performance than solid carbon nanocube (SCNC)-coated fiber (denoted as SCNCs-F) and commercial fibers based on the abundant active sites of the hollow structure, hydrophobic interactions, and π–π interactions. Moreover, because of the reduced mass-transport lengths of the hollow mesoporous structure, the HCNCs-F demonstrated a faster mass transfer compared with the SCNCs-F. The HCNCs-F was used to determine the six hydrophobic polycyclic aromatic hydrocarbons (PAHs) with wide linear ranges (10–2000 ng L–1 for naphthalene and 5–2000 ng L–1 for the other five analytes), good reproducibility (relative standard deviation < 8.8%), and low detection limits (0.03–0.70 ng L–1). Finally, the HCNCs-F was successfully applied for the determination of PAHs from the real water samples. It can be concluded from the results that MOF-derived hollow carbon materials are promising candidates for the fast SPME and can be used for practical applications in analytical chemistry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Lili Chen,Yuanyuan Zheng,Longbao Feng,Zonghua Liu,Rui Guo,Yuanming Zhang
The aim of this work was to examine the formation and properties of a novel polyelectrolyte complex of drug carrier system for the delivery of doxorubicin (DOX), which consists of hyaluronic acid (HA) coated hydrophobically modified chitosan (CS). Various batches of polyelectrolyte complexes with the molar ratio of deoxycholic acid (DCA) and chitosan (CS) of 0.1, 0.2, 0.3 were prepared, and were termed as CS-DCA 10 , CS-DCA 20 , and CS-DCA 30 respectively. The samples were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), Transmission electron microscopy (TEM), nuclear magnetic resonance hydrogen spectrum ( 1 H NMR) and dynamic light scattering (DLS). Particle sizes of synthesized polyelectrolyte complex nanoparticles (PCNs) were found to be in the range of 280–310 nm, larger than those of uncoated nanoparticles (~150 nm). The PCNs have large zeta potentials (about 26 mV) which make them stable and no sizes' change was determined. DOX could be easily incorporated into the PCNs with encapsulation efficiency (56%) and kept a sustained release manner without burst effect when exposed to PBS (pH 7.4) at 37 °C. Overall, these findings confirmed the potential of these PCNs for drug carrier and prolonged and sustained delivery in the bloodstream.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2018
·
IF 1.712
Xiaoqiang Jia,Yun He,Lei Huang,Dawei Jiang,Wenyu Lu
The high-molecular weight polycyclic aromatic hydrocarbons (PAHs) pyrene and typical long chain alkane n -hexadecane are both difficult to degrade. In this study, n -hexadecane and pyrene degrading strain Rhodococcus sp. T1 was isolated from oil contaminated soil. Strain T1 could remove 90.81% n -hexadecane (2 vol%) and 42.79% pyrene (200 mg·L − 1 ) as a single carbon within 5 days, respectively. Comparatively, the degradation of pyrene increased to 60.63%, but the degradation of n -hexadecane decreased to 87.55% when these compounds were mixed. Additionally, identification and analysis of degradation metabolites of Rhodococcus sp. T1 in the above experiments showed that there were significant changes in alanine, methylamine, citric acid and heptadecanoic acid between sole and dual substrate degradation. The optimal conditions for degradation were then determined based on analysis of the pH, salinity, additional nutrient sources and liquid surface activity. Under the optimal conditions of pH 7.0, 35 °C, 0.5% NaCl, 5 mg·L − 1 of yeast extract and 90 mg·L − 1 of surfactant, the degradation increased in single or dual carbon sources. To our knowledge, this is the first study to discuss metabolite changes in Rhodococcus sp. T1 using sole substrate and dual substrate to enhance the long-chain alkanes and PAHs degradation potential.
✓ 已验证
产品关联
DOI
高影响因子
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
·
2018
·
IF 1.945
Nian-Qiu Shi,Hong-Wei Lai,Yong Zhang,Bo Feng,Xiao Xiao,Hong-Mei Zhang,Zheng-Qiang Li,Xian-Rong Qi
Polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer, or Soluplus®, is a relatively new copolymer and a promising carrier of amorphous solid dispersions. Knowledge on the inherent properties of Soluplus® (e.g. cloud points, critical micelle concentrations, and viscosity) in different conditions is relatively inadequate, and the application characteristics of Soluplus®-based solid dispersions made by microwave methods still need to be clarified. In the present investigation, the inherent properties of a Soluplus® carrier, including cloud points, critical micelle concentrations, and viscosity, were explored in different media and in altered conditions. Ibuprofen, a BCS class II non-steroidal anti-inflammatory drug, was selected to develop Soluplus®-based amorphous solid dispersions using the microwave-quench cooling (MQC) method. Scanning electronic microscopy (SEM), differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD), Raman spectroscopy (RS), and Fourier transform infrared spectroscopy (FT-IR) were adopted to analyze amorphous properties and molecular interactions in ibuprofen/Soluplus® amorphous solid dispersions generated by MQC. Dissolution, dissolution extension, phase solubility, equilibrium solubility, and supersaturated crystallization inhibiting experiments were performed to elucidate the effects of Soluplus® on ibuprofen in solid dispersions. This research provides valuable information on the inherent properties of Soluplus® and presents a basic understanding of Soluplus® as a carrier of amorphous solid dispersions.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 20
·17091-17095
·
IF 3.201
Youyou Pang,Rujian Zhao,Yao Lu,Jiyang Liu,Xiaoping Dong,Fengna Xi
Simple anti-counterfeiting printing using biocompatible, non-toxic and highly efficient luminescent materials plays crucial roles in economic and social fields as well as in our daily lives. In this work, new nitrogen doped graphene quantum dots (N-GQDs) are synthesized and applied in the preparation of quick-dry fluorescent ink for both writing and printing. The N-GQDs are synthesized based on a one-step bottom-up molecular fusion between 1,3,6-trinitropyrene and triethylamine by a solvothermal process with a high yield of 84.6%. Triethylamine simultaneously acts as a reaction medium and a nitrogen-doping agent for the formation of fluorescent N-GQDs. The as-prepared N-GQDs exhibit a single-layer graphene structure, high crystallinity, uniform size and excitation-independent fluorescence emission with a narrow half-peak width (66 nm). Successful doping of N atom in GQDs enables bright blue fluorescence with a high absolute photoluminescence quantum yield (17.8%). Owing to their excellent photostability and good solubility in ethanol, N-GQDs are applied to easily prepare quick-dry fluorescent ink for both writing and printing by simply blending GQDs with glycerol in an ethanol medium. Moreover, synthesis of a fluorescent powder and hydrogel is also achieved.
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2018
·
IF 13.709
Xuanrong Sun,Guowei Wang,Hao Zhang,Shiqi Hu,Xin Liu,Jianbin Tang,Youqing Shen
Polymer micelles are one of the most investigated nanocarriers for drug delivery; many have entered clinical trials and some are in clinic use, but their delivery systems have not yet shown the expected high therapeutic efficacy in clinics. Further understanding their in vivo behaviors, particularly how quickly and by what mechanism polymer micelles are cleared (i.e., via micelles or unimers) once injected, is key to solving this dilemma. Herein, we hope to answer these questions for the clinically relevant polyethylene glycol-block-poly(ε-caprolactone) (PEG-PCL) and PEG-block-poly(d,l-lactide) (PEG-PDLLA) micelles. A small fraction of the hydrophobic chain ends was conjugated with a pair of fluorescence resonance energy transfer (FRET) dyes, Cy5 and Cy5.5, and used to fabricate FRET micelles whose FRET efficiency was correlated to the percentage of polymer chains in the micelles, the micelle degree. In vitro, serum proteins induced PEG-PCL micelle dissociation to some extent; mouse serum or blood surprisingly did not induce micelle dissociation but once with shear applied by a microfluidic channel caused most PEG-PCL micelles dissociated. After intravenous administration in mice, the PEG-PCL or PEG-PDLLA micelles were quickly sequestered into the liver as unimers, and the micelle degree in the blood quickly decreased to about 20%. The FRET-imaging experiments showed that in blood vessels the micelles quickly dissociated into unimers, which were found associated with albumin in blood and in liver. Thus, it is concluded that, upon intravenous injection, the shear and the bloodborne proteins (particularly albumin) induced the most (∼80%) PEG-PCL and PEG-PDLLA micelles to quickly dissociate into unimers, which were sequestered by Kupffer cells, while intact micelles were difficult to clear. These micelles were able to penetrate tumors and were very stable with cell membranes, but dissociated gradually inside cells. These findings on in vivo micelle fate and the clearance mechanism are directional for the rational design of polymer micelles for improved therapeutics; particularly, improving micelle stability in blood is the prerequisite for surface functionalizations such as introducing targeting ligands.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2018
·Vol. 135
·期 5
·2903-2913
·
IF 2.209
Wang Yujie,Wu Hui,Wang Jiuxia,Lou Pengxiao,Zhao Yang,Bai Guangyue
Thermodynamics of interaction between cationic gemini surfactants [C 12 H 25 (CH 3 ) 2 N(CH 2 ) s N(CH 3 ) 2 C 12 H 25 ]Br 2 , ( s = 2, 6 or 10, assigned as C 12 C s C 12 Br 2 ) and sodium deoxycholate (NaDC) in aqueous solution has been investigated by isothermal titration calorimetry (ITC), fluorescence and turbidity measurements. The critical micelle concentrations for NaDC-rich mixture (cmc mix,12-s-12 ) were determined by fluorescence method. The cmc mix,12-s-12 values are much smaller than those for pure C 12 C s C 12 Br 2 surfactants, and decrease as the spacer length ‘s’ increases. There is a strong synergistic effect between two surfactants in the NaDC-rich region. In the higher C 12 C s C 12 Br 2 concentration range above cmc mix,12-s-12 , we did obtain from ITC and turbidity measurements the critical parameters for different events that occur as the C 12 C s C 12 Br 2 concentration increases, including the critical concentrations ( C P , C R , C M ) of formation and redissolution for LC of the mixed C 12 C s C 12 Br 2 /NaDC systems and transition to gemini-rich micelles. In comparison with the mixed C 12 C s C 12 Br 2 /NaC systems reported with sodium cholate (NaC), the mixed systems with NaDC have lower cmc mix,12-s-12 , C P , C R and C M , and thus different self-aggregation processes are well controlled by surfactant concentration and molar fraction. Importantly, ITC allowed us to directly measure the enthalpy changes corresponding to different self-aggregation processes—redissolution enthalpy (∆ H R ) of LC of mixed surfactants and transition enthalpy (∆ H M ) from LC to gemini-rich micelles. Eventually, we discussed the cross-linking effect on NaDC-rich micelles of the gemini surfactant (C 12 C s C 12 Br 2 ) with double hydrophobic chains and cationic head groups linked through a methylene spacer.
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 46
·5516-5523
·
IF 2.073
Qing Wang,Jiaxin Lian,Zhongdan Hua,Ying Yang,Ying Li,Xiuwen Hao,Yaxin Cao,Xianqi Zeng
A novel magnetic solid-phase extraction (MSPE) sorbent, magnetic multiwalled carbon nanotube-octadecylphosphonic acid modified zirconia (mMWCNT–ZrO2–C18) composite, was fabricated via a simple solvothermal reduction method to extract polycyclic aromatic hydrocarbons (PAHs) from edible oils, coupling with high performance liquid chromatography analysis. The hydrophobic ZrO2–C18 nanoparticles were introduced to enhance the extraction capability. Factors influencing the extraction efficiency, i.e. the type and volume of desorption solvent, amount of the sorbent, and adsorption and desorption time, were systematically investigated. Under the optimal conditions, calibration curves were linear (R2 > 0.9937) over the concentration range from 0.20 to 200 ng g−1. The limits of detection (S/N = 3) and limits of quantification (S/N = 10) achieved were in the range of 0.06–0.55 and 0.20–1.83 ng g−1, respectively. The intra- and inter-day relative standard deviations were less than 7.21%. A comparative study indicated that higher recoveries were obtained by using mMWCNT–ZrO2–C18 compared to when using bare mMWCNTs. The results indicated that the mMWCNT–ZrO2–C18 nanomaterial was endowed with great adsorption capability and had significant potential in the preconcentration of PAHs from edible oil samples.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）