Food Bioengineering
·
2024
·Vol. 3
·期 2
·151-159
·
IF 0
Ziye Zhao,Ning Nie,Xinwei Li,Ye Shi,Xudong Liu,Yifei Zhang
Nucleotides, such as uridine 5′-monophosphate (5′-UMP) and cytidine 5′-monophosphate (5′-CMP), are heavily demanded as food additives, dietary supplements, and medicinal intermediates. Although enzymatic phosphorylation is an effective approach to producing these nucleosides, its practical application is challenging due to the need for the expensive enzymes and coenzyme guanosine-5′-triphosphate (GTP). Here we prepared biocompatible calcium-based nanoflowers incorporated with cascade enzymes, uridine-cytidine kinase (UCK) and acetate kinase (ACK), for the production of nucleotides. The spatial distribution of these enzymes was optimized to maximize their catalytic performances. The most active nanoflowers (Ca 3 (PO 4 ) 2 & ACK)& UCK that display the rate-limiting enzyme UCK on the outer layer were used in a packed-bed reactor for continuous synthesis of 5′-UMP and 5′-CMP. The catalytic performance of the catalyst retained over 80% within 10 h, showing good operational stability.
研究领域:
生物催化
·
纳米技术
·
酶工程
·
生物化学
·
食品科学
·
药物化学
✓ 已验证
DOI
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Dongmei Hao,Yanbin Sun,Dezhong Xie,Fuhao Chu,Weiguang Wang,Anran Zhou,Chen Chen,Yong Wang,Hongying Lü
The oxidation of 5-hydroxymethylfurfural (HMF) to produce high value-added 5-formyl-2-furancarboxylic acid (FFCA) represents a promising biomass conversion reaction that holds potential for biomass-based material for the drug synthesis and polyester industry. The present study presents a metal-free and alkali-free catalytic system consisting of N-hydroxyphthalimide (NHPI) as an organic molecule and a deep eutectic solvent (DES). This system was successfully employed for the aerobic oxidation process of HMF to FFCA, demonstrating exceptional catalytic activity with 100% conversion and 96% FFCA yield in the absence of any alkali. Multiple hydrogen bond interactions were found to be indispensable and play a crucial role in promoting the catalytic process. These H-bond interactions serve three essential purposes: facilitating the formation of deep eutectic solvents (DESs) between polyethylene glycol and tetrabutylammonium chloride, accelerating the dissolution and activation of HMF, and aiding in the generation of active free radicals for NHPI. This work demonstrated the predominant oxidation ability of the NHPI-DESs system for the oxidation of HMF to FFCA through hydrogen bond interaction, thereby offering invaluable insights into the aerobic oxidation processes involving hydrocarbons or alcohols.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2026
·Vol. 91
·期 32
·11063-11070
·
IF 3.3
Zhang Tongtong,Wang Ning,Yang Wenjing,Guo Zhen
Herein, we report a novel, photocatalyst-free protocol
enabling
the efficient synthesis of diverse 5-amino-1,2,4-thiadiazoles from
readily available benzamidines hydrochloride and isothiocyanates.
This transformation proceeds via a cascade C–N/N–S bond
formation. Mechanistic studies, including radical trapping experiments,
control reactions, and UV–vis spectroscopy, indicate that the
key step involves photoinduced oxidation of an in situ-generated thiolate
anion, yielding a critical sulfur-centered radical. The reaction exhibits
broad substrate scope and excellent functional group tolerance and
takes place without the need of any chemical oxidants and catalysts,
thus providing a simpler, more cost-effective, and greener synthetic
route for the construction of thiadiazoles.
✓ 已验证
DOI
PubMed
高影响因子
ChemCatChem
·
2026
·Vol. 18
·期 9
·e70777
·
IF 4.1
Weidong Zhao,Xiedong Li,Fu Zhao
The valorization of biomass-derived platform molecules driven by renewable electricity is widely regarded as a green and sustainable approach, in which the rational design of electrocatalysts is crucial for achieving efficient and selective conversion. Herein, a series of CuAg heterostructured electrocatalysts was constructed in situ on copper foam (CF) substrates via a two-step electrodeposition method for the electrocatalytic hydrogenation of 5-hydroxymethylfurfural (HMF) to 2,5-bis(hydroxymethyl)furan (BHMF). Experimental results demonstrate that initial Cu deposition plays an important role in increasing the electrochemically active surface area (ECSA) and enhancing interfacial synergy. Subsequent introduction of Ag significantly lowers the onset overpotentials for both the hydrogen evolution reaction (HER) and the electrocatalytic hydrogenation (ECH) of HMF, thereby accelerating the overall reaction kinetics and effectively suppressing the dimerization side reaction. The optimized CuAg/CF-2 catalyst achieves 92.1% HMF conversion and 88.9% BHMF selectivity within only 1 h of electrolysis, with a BHMF productivity of 0.164 mmol cm −2 h −1 . Density functional theory calculations reveal that Ag incorporation modulates the electronic structure of Cu sites and constructs a CuAg heterointerface with characteristic geometric features, which facilitates the generation of adsorbed hydrogen (H*), enhances the adsorption and activation of HMF, while simultaneously promoting BHMF desorption and suppressing competitive HER. Graphical Driven by renewable electricity, biomass-derived molecules can be valorized in a green and sustainable manner via electrocatalytic pathways. In this work, CuAg heterostructured electrocatalysts (CuAg/CF) constructed on copper foam (CF) substrates enable efficient and highly selective electrocatalytic hydrogenation of 5-hydroxymethylfurfural (HMF) to 2,5-bis(hydroxymethyl)furan (BHMF), highlighting the potential of heterointerface engineering in biomass electrocatalytic conversion.
✓ 已验证
DOI
高影响因子
JOURNAL OF CHEMICAL SCIENCES
·
2026
·Vol. 138
·期 4
·118
·
IF 1.8
Xu Guohao
A Ga-substituted ZSM-5 zeolite was successfully synthesized via a hydrothermal synthesis method, and its catalytic performance and physicochemical properties in propane aromatization were systematically investigated. Comprehensive characterizations including XRD, SEM, ICP-AES, IR, NH 3 -TPD, XPS, N 2 adsorption-desorption and TG analyses revealed that Ga species exhibited the lower tendency to incorporate into the ZSM-5 zeolite framework compared to Al species. The gallium-doped aluminosilicate (GaAlZ5), possessing the largest specific surface area and highest acid content, demonstrated a superior aromatic selectivity of 85.6% and propane conversion of 66.1%, which exceeded those of gallosilicate (GaSiZ5) by 22.5% and 5.6%, respectively. Moreover, the intergranular mesopores in GaAlZ5 enhanced the mass-transfer rate of aromatics, effectively suppressed the formation of coke precursors, and endowed the catalyst with excellent long-term stability as well as regeneration stability. This work provides a facile synthesis strategy and systematic theoretical support for the design of high-stability and high-selectivity Ga-based zeolite catalysts for propane aromatization. Graphical abstract This graphical abstract illustrates the bifunctional tandem pathway of propane aromatization, where propane is activated to propylene via dehydrogenation over Ga-based Lewis acid sites, followed by aromatization under the synergy of Brønsted and Lewis acid sites. This work reports a Ga-modified ZSM-5 zeolite (GaAlZ5) with optimized acid properties and pore structure, which delivers 66.1% propane conversion and 85.6% aromatics selectivity with excellent recyclability, providing a facile approach for light alkane valorization.
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
·
IF 9.1
Shi-Jie Jiang,Peng-Xiao Lv,Xing Chen,Ying-Hao Wang,Yan-Hui Chong,Qian Zhang,Jing-Lan Feng,Hai-Ming Guo
Due to their extensive use and widespread distribution, per - and polyfluoroalkyl substances (PFAS) pose significant risks human health and the environment. Development of efficient and environmentally benign adsorbents is urgently needed. In this study, three fluorine-free polymers (labeled QA2P-P , QA4P-P , and QA6P-P ) with cationic binding sites were constructed from quaternized pillar[5]arenes conveniently. These polymers exhibited excellent removal performance for three representative PFAS (perfluorooctanoic acid [PFOA], perfluoropentanoic acid [PFPeA], and ammonium perfluoro-2-propoxypropionate [GenX]). Notably, QA2P-P exhibited >99% removal efficiency for PFOA and GenX. Furthermore, QA2P-P reduced PFOA and GenX from 1 μg L −1 to <4 and < 10 ng L −1 , respectively. The maximum adsorption capacity of QA2P-P was 868.9 mg g −1 for PFOA, 399.6 mg g −1 for GenX, and 452.1 mg g −1 for PFPeA. These results positioned QA2P-P ahead of all previously reported fluorine-free materials and make it comparable to outstanding fluorinated adsorbents. Electrostatic and hydrophobic interactions worked together to enhance the removal capacity. All the polymers were easily synthesized, stable, and readily regenerated. The present research provided valuable guidance for the design of fluorine-free and efficient adsorbents for PFAS.
✓ 已验证
DOI
高影响因子
NEUROENDOCRINOLOGY
·
2023
·Vol. 113
·期 6
·641-656
·
IF 4.1
Zheng Biaolin,Chen Xiubing,Chen Fengping,Liao Xiaomin,Wang Feng,Yang Zhe,Yi Nan,Shi Xiaoyan,Qin Shanyu,Jiang Haixing
Introduction: 5-Iodotubercidin, a type of purine derivative, has attracted increasing attention in tumor chemotherapy because of its potential as an antitumor agent in recent years. In this study, we confirmed the effects on apoptosis in insulinoma cell lines induced by 5-iodotubercidin and tried to illuminate the underlying mechanisms. Methods: We used 5-iodotubercidin in the treatment of insulinoma cells and the cell proliferation was examined using CCK-8 assay, colony-forming assays, and insulinoma animal models. Cell apoptosis was examined using TUNEL assays and Western blotting. Cellular DNA damage was shown by comet assay and immunofluorescence. The expression of apoptosis-regulating proteins and DNA damage biomarker was investigated by Western blotting. Subcutaneous inoculation of the insulinoma cells into nude mice was to measure blood glucose, insulin levels, and tumor growth. ATM siRNA and p53 siRNA were used as loss-of-function targets to evaluate 5-iodotubercidin treatment. Results: 5-Iodotubercidin inhibited the proliferation of insulinoma cells and induced DNA damage and cell apoptosis. Moreover, 5-iodotubercidin induced ATM and p53 activated. In vivo, 5-iodotubercidin inhibited the growth of Ins-1 and Min-6 cells xenografts in nude mice. Conclusion: 5-Iodotubercidin induces DNA damage leading to insulinoma cells apoptosis by activating ATM/p53 pathway. Therefore, this is a potential strategy for treating insulinoma
✓ 已验证
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2023
·
IF 3.4
Feifei Song,Tao Gu,Lin Zhang,Jiaxing Zhang,Shengping You,Wei Qi,Rongxin Su
5-Hydroxytryptophan (5-HTP) is a chemical precursor of serotonin, which synthesizes melatonin and serotonin in animals and regulates mood, sleep , and behavior. Tryptophan hydroxylase (TPH) uses tetrahydrobiopterin (BH4) as a cofactor to hydroxylate L -tryptophan ( L -Trp) to 5-HTP, and the low catalytic activity of TPH limits the rate of hydroxylation of L -Trp. In this study, the catalytic mechanism and structural features of L -Trp-TPH1-BH4 were investigated, and the catalytic activity was improved using a rational design strategy. Then the S337A/F318Y beneficial mutation was obtained. Molecular dynamics simulations showed that the S337A/F318Y mutant formed a salt bridge with TPH1 while forming an additional hydrogen bond with the substrate indole ring, stabilizing the indole ring and enhancing the binding affinity of the variant to L -Trp. As a result, the yield of 5-HTP was increased by 2.06-fold, resulting in the production of 0.91 g/L of 5-HTP. The rational design of the TPH structure to improve the hydroxylation efficiency of L -Trp offers the prospect of green production of 5-HTP.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2023
·
IF 22.1
Kezhen Qian,Wenmin Tian,Lijie Yin,Zixu Yang,Feixiang Tian,Dezhen Chen
The nature of Zn species and their roles in catalytic cracking of high-density polyethylene (HDPE) thermal decomposing vapor over Zn-promoted HZSM-5 (Zn/Z5) for aromatics were investigated. The Zn/Z5 zeolites were prepared using ion exchange and impregnation methods followed by H 2 reduction. Results indicated that hydrogen reduction of the Zn/Z5 significantly improved the aromatic yield. [ZnOH] + and bridged Zn 2+ species were critical species promoting BTX formation, with the former demonstrating higher activity. Besides, the synergistic effect between the Zn species and Brønsted acid sites on HZSM-5 boosts the aromatic yield. Incorporation of Zn was also found to promote long-chain scission. Among all the Zn/Z5, hydrogen reduced 2 wt% Zn/Z5 (SAR=25) is most effective in producing BTX, with a maximum yield of BTX (48%) and BTX selectivity (93%); hydrogen reduced 2 wt% Zn/Z5 (SAR=50) produced the highest yield of total aromatics (53%).
✓ 已验证
产品关联
DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2022
·
IF 5.876
Xiuli Shao,Siqi Wang,Youhui Zhou,Xuan Zhang,Haizhou Tian,Zheng Wang,Zhong-Yong Yuan,Huanting Wang
The ZSM-5 zeolite nanosheets are highly desired materials for catalytic reactions and membrane separation . Their synthesis depends on specially designed complex surfactants ; however, the high-cost limits their large-scale industrial production. Herein, the tailored synthesis of coffin-shaped multilamellar ZSM-5 composed of orderly stacked 2D nanosheets is carried out using cetyltrimethylammonium bromide (CTAB) as a structure-directing agent (SDA) in the presence of silicalite-1 seed sol. The morphology and b -axis thickness of nanosheets is controlled by adjusting the amount of silicalite-1 seed sol, CTAB/TEOS ratio, SiO 2 /Al 2 O 3 ratio, and synthesis time. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis indicate that the hydrophobic tail of CTAB acts as an inhibitor, effectively preventing the continuous growth along b -direction with an average thickness of 20–100 nm. This work highlights the role of silicalite-1 seed sol and CTAB in the orderly stacked sheets growth process. This crystallization strategy opens a facile and inexpensive approach for multilamellar ZSM-5 zeolite fabrication with 2D-nanosheets structures, and their practical applications in catalytic conversions of bulky molecules.
✓ 已验证
产品关联
DOI
高影响因子
Reaction Kinetics Mechanisms and Catalysis
·
2022
·Vol. 135
·期 4
·1957-1970
·
IF 1.843
Wang Chengqian,Zhao Caiyi,Zhang Long
To enhance the selective fructose dehydration to 5-hydroxymethylfurfural (HMF), an efficient dehydration of fructose to 5-HMF in H 2 O-MIBK- pressured CO 2 in 20 min at 200 °C with a better product yield of 85.6% and 89.1% product selectivity was achieved. The high activity attributed to the combination of the weak acidity by CO 2 dissolved in the water and the efficient separation of fructose with 5-HMF in this H 2 O-MIBK two-phase system. The possible reaction mechanism is discussed. It was found that the solvents ratio was the one of key factors determining the yield of target products. Compared to the related reported results in the reaction, this process characterized of more favorable product yield and higher selectivity, it can be considered as a potential technique for the scale-up production of 5-HMF from fructose.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS RESEARCH
·
2022
·Vol. 37
·期 2
·543-553
·
IF 2.909
Jia Yanfei,Wei Jianwen,Yuan Yuan,Geng Linlin,Chen Siqi,Liao Lei
A stable and effective amine-modified adsorbent for CO 2 capture was prepared with the impregnation method using the ZSM-5/SBA-16 (ZS) as support and the tetraethylenepentamine (TEPA) as modifier. The CO 2 adsorption performance was investigated by thermal gravimetric analysis (TGA) in mixed gases (15% CO 2 and 85% N 2 ) at 30–90 °C. At 60 °C, the ZS displayed the highest CO 2 adsorption capacity of 2.80 mmol g −1 at 60% TEPA loading. The adsorption kinetics analysis showed that the adsorption process of amine-modified ZS was dominated by both physical and chemical adsorptions. After five cycles of adsorption/desorption, ZS-T-60 kept better stability with the equilibrium adsorption capacity of the composite material was 2.486 mmol g −1 , which was 11.1% lower than the original adsorption capacity. The composite material ZS showed good CO 2 capture ability, indicating the promising future of these adsorbents for CO 2 adsorption from actual flue gas after desulfurization. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）