JOURNAL OF PROTEOME RESEARCH
·
2025
·
IF 3.6
Shu-Jun Gu,Liu Xu,Hong-Kun Zhou,Jie Yang
Pancreatic ductal adenocarcinoma (PDAC) with negative carbohydrate antigen 19-9 (CA19-9) lacks effective diagnostic and monitoring indicators. We aimed to identify metabolic characteristics in such patients through metabolomics and explored potential plasma biomarkers. In this study, untargeted metabolomics analysis was performed on plasma samples from 30 healthy controls and 30 CA19-9 positive and 30 CA19-9 negative PDAC patients by using liquid chromatograph–mass spectrometer (LC–MS) technology. Compared with healthy controls, 36 differential metabolites were upregulated, and 3 differential metabolites were downregulated in the plasma of CA19-9 negative PDAC patients. Four upregulated differential metabolites, including palmitic amide, dolichol phosphate, cholesteryl acetate, and vitamin A2 aldehyde, were potential biomarkers for diagnosing CA19-9 negative PDAC patients. Enrichment analysis of KEGG pathways showed that glycine and threonine metabolism, tyrosine and tryptophan biosynthesis, thiamine metabolism, etc., were disrupted in CA19-9 negative PDAC patients. In summary, we determined the metabolic heterogeneity in PDAC patients with normal CA19-9 levels. The detected metabolic biomarkers may offer new approaches for the clinical early diagnosis of CA19-9 negative PDAC in the future.
研究领域:
肿瘤学
·
代谢组学
·
临床诊断
·
分子生物学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2012
·Vol. 30
·期 7
·1571-1574
·
IF 0.755
Jing Cao,Yang Li,Junxiang Feng
Two kinds of novel styryl chemosensory 2-FMNC and 3-FMNC, were designed and synthesized by an apporiate introduction of 9-benzylidene-9 H -fluorene group as fluorophore with the aim at avoiding photoisomerisation. These 9-benzylidene-9 H -fluorene derivatives showed the similar selectivity and sensitivity upon addition of metal ions. The sensitivity of FMNC to alkaline earth metal ions was Ba 2+ >Sr 2+ >Ca 2+ ≈Mg 2+ .
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DOI
高影响因子
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
·
2021
·Vol. 18
·期 12
·2531-2538
·
IF 0.533
Jun Tang,Jingfang Yang
Purpose: To investigate the potential role of curcumin in osteosarcoma (OS) cell migration and invasion, and also to explore the underlying mechanism of action.
Methods: OS cell lines, Saos-2 and U2OS, were treated with various concentrations of curcumin (0, 10, 20, 50 μM) for 48 h. Transwell assay was used to determine the migratory and invasive activities of human OS cells while miR-33b-5p expression levels were measured using quantitative real time polymerase chain reaction (qRT-PCR). Expressions of metastasis-related genes, such as matrix metalloproteinases (MMPs), were quantified by qRT-PCR or western blot. The interaction between miR33b-5p and Sirtuin 6 (SIRT6) was evaluated by luciferase assay.
Results: Curcumin inhibited the migratory and invasive activities of huamn OS cells, and downregulated MMP2 and MMP9 (p < 0.05). Moreover, in curcumin-treated OS cells, miR-33b-5p expression was upregulated while SIRT6 expression was significantly downregulated (p < 0.05). Overexpression of miR-33b-5p downregulated SIRT6 mRNA and protein expression, whereas transfection with miR-33b-5p inhibitor upregulated SIRT6 expression in OS cells (p < 0.05). Furthermore, curcumin suppressed SIRT6 expression by upregulating miR-33b-5p expression in OS cells. Finally, overexpression of SIRT6 or miR-33b-5p inhibitor reversed the suppression of OS cell metastasis induced by curcumin (p < 0.05).
Conclusion: Curcumin inhibits SIRT6 expression via upregulating miR-33b-5p expression, which leads to the inhibition of OS cell migration and invasion. Thus, curcumin is a potential therapeutic agent for the management of OS.
研究领域:
肿瘤学
·
分子生物学
·
药理学
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DOI
高影响因子
Asian Journal of Pharmaceutical Sciences
·
2026
·
IF 12.6
Chenxiao Chu,Xi Chen,Yaxin Deng,Jie Zhang,Xiaoshuang Bi,Yunfeng Ma,Mingli Wei,Jiansong Zhao,Haibing He,Jingxin Gou,Tian Yin,Xing Tang,Li Yang,Yu Zhang
Corneal neovascularization (CNV), an inflammation-driven pathological process responsible for irreversible vision loss, remains an urgent unmet challenge in ophthalmic medicine. Current interventions—ranging from surgery to topical steroids—fail to prevent recurrence or evade severe side effects like intraocular hypertension and even corneal ulceration. Here, we introduce CL-Peptide-Dex, a transformative contact lens (CL) engineered for matrix metalloproteinase-9 (MMP-9)-responsive dexamethasone (Dex) release, to resolve this decades-long challenge. By grafting dexamethasone on the lens surface via an MMP-9-cleavable peptide linker (GPLGLAGC), CL-Peptide-Dex achieves disease-adaptive drug delivery: rapid release during acute inflammation and sustained release throughout CNV progression. Unlike conventional drug-eluting systems, this surface-grafting strategy eliminates burst release while preserving lens transparency and oxygen permeability. In the rabbit corneal neovascularization model, a single administration of CL-Peptide-Dex could maintain a stable Dex level for 7 days, suppressed VEGF by 77.5%, and reduced neovascular area by 79.5%. Critically, CL-Peptide-Dex avoided intraocular pressure spikes and exhibited good corneal biocompatibility and compliance, enabling safe extended wear. By integrating MMP-9-responsive precision delivery with patient-friendly design, CL-Peptide-Dex represents a significant advance in precision ophthalmology.
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2026
·
IF 4
Shan Ye,Chenxi Xiang,Beibei Luo,Ruoyu Zhang,Yao Wang,Jinglei Chen,Zhuhong Yang,Zhong Ding
The rice root-knot nematode Meloidogyne graminicola causes severe yield losses in rice production, requiring eco-friendly control measures. Here, we isolated Enterobacter asburiae SA-9 from rice rhizosphere soil and evaluated the biocontrol potential of its volatile organic compounds (VOCs) against M. graminicola . In vitro, SA-9 VOCs exhibited potent fumigant activity, causing high mortality of second-stage juveniles (J2s) and significant inhibition of egg hatching. Chemotaxis assays showed that SA-9 disrupted nematode chemotaxis through repellent activity. Further investigations revealed that exposure to SA-9 VOCs significantly downregulated key chemosensory genes ( Mg-odr-1 , Mg-odr-3 , Mg-osm-9 , Mg-tax-4 ) in J2s, impairing their host-seeking and infection abilities. In double-layered pot, SA-9 VOCs effectively reduced root gall index and nematode populations, while concurrently promoting rice growth, accompanied by upregulation of auxin ( OsAUX1 , OsYUCCA1 ) and gibberellin ( OsGID1 ) biosynthesis-related genes. Furthermore, SA-9 VOCs induced genes related to systemic resistance in rice, as evidenced by the increased expression of defense-related genes in the salicylic acid ( OsPR1a , OsWRKY45 ) and jasmonic acid ( OsAOS2 , OsMYC2 ) signaling pathways. SPME-GC–MS profiling identified 12 VOCs produced by SA-9, with 2-phenylethanol, 2-methylbutyric acid, isobutyric acid and propanoic acid exhibiting significant contact and fumigation nematicidal activities against J2s. These findings demonstrate that SA-9 VOCs suppress M. graminicola through multiple mechanisms, highlighting their potential as sustainable biocontrol agents for managing root-knot nematodes in rice agroecosystems.
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DOI
PubMed
高影响因子
JOURNAL OF INORGANIC BIOCHEMISTRY
·
2026
·
IF 3.1
Zhijian Zhang,Zhongshan Zhou,Wujiu Jiang,Yuxing Tan
Four organotin acridine-9-carboxylate complexes ( C1 – C4 ) were successfully synthesized by the reaction of 9-acridinecarboxylic acid ( YCOOH ) with organotin precursors. The structures of these complexes were systematically characterized using elemental analysis, IR, NMR, X-ray single-crystal diffraction, X-ray powder diffraction and thermogravimetric analysis. Among them, the tributyltin complex C4 exhibited the most potent in vitro antiproliferative activity against HepG2, MCF-7, and NCI-H460 cancer cell lines, with IC 50 values as low as 0.08 μM against HepG2 cells. Mechanistic studies identified two functional pathways: (i) DNA intercalation, as evidenced by UV–Vis, fluorescence titrations, viscosity measurements, and molecular docking, which directly disrupts DNA architecture; and (ii) mitochondria-mediated apoptosis, characterized by collapse of mitochondrial membrane potential (Δ Ψ m ), cytochrome c (Cyt C) release, elevated ROS generation, upregulation of Bax and Cleaved-caspase-3, and downregulation of Bcl-2. Importantly, these two pathways act synergistically: DNA intercalation triggers early nuclear stress, which amplifies mitochondrial dysfunction and ROS burst, thereby lowering the apoptotic threshold and enhancing the overall cytotoxic efficacy. Collectively, the dual DNA-mitochondria targeting and their cooperative action establish acridine-based organotin complexes as promising anticancer candidates.
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Yiran Tian,Xiuting Li,Fuyan Wang,Chenjie Gu,Ziqi Zhao,Hongjie Si,Tao Jiang
Here, straight upward Au nanowires (NWs) were successfully grown onto Fe 3 O 4 @TiO 2 matrix through a seed-mediated strategy to intensively improve its photocatalysis and SERS performances, facilitating a peculiar recyclable surface-enhanced Raman spectroscopy (SERS)-based immunoassay of CA19–9 in liquid form based on visible light irradiation. Such immunoassay was also supported by a smart heterobifunctional cross-linking agent-mediated probe of anti-CA19–9/4-MBA without metal nanoparticles . A low limit of detection of 5.65 × 10 −4 IU mL −1 and a wide linear range from 1000 to 0.001 IU mL −1 were demonstrated through repeated constructing the sandwich immunostructure with only one batch of nanocomposites . Moreover, the actual levels of CA19–9 for colorectal cancer patients were readily measured by the recyclable immunoassay, the results of which are principally consistent with the conventional CLIA detection. Thus, such a green strategy of visible light-induced recyclable immunoassay could be expected to have a potential practicability in the clinical diagnoses of cancer.
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DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 20
·6778
·
IF 4.927
Zhengzheng Yan,Jun Xing,Ruochong He,Qinping Guo,Ji Li
Convenient and sensitive detection of tumor biomarkers is crucial for the early diagnosis and treatment of cancer. Herein, we present a probe-integrated and label-free electrochemical immunosensor based on binary nanocarbon composites and surface-immobilized methylene blue (MB) redox probes for detection of carbohydrate antigen 199 (CA19-9), which is closely associated with gastric malignancies. Nanocarbon composites consisting of electrochemically reduced graphene oxides and carbon nanotubes (ErGO-CNT) are electrodeposited onto an indium tin oxide (ITO) electrode surface to form a 3D nanocomposite film, which could provide high surface area to immobilize abundant MB probes, facilitate the electron transfer of MB, and therefore, improve sensitivity. Polydopamine (PDA) served as a bifunctional linker is able to immobilize anti-CA19-9 antibodies and stabilize the inner probe, conferring the sensing interface with specific recognition capacity. Electrochemical detection of CA19-9 is achieved based on the decrease of the redox signal of MB after specific binding of CA19-9 with a wide linear range of 0.1 mU/mL to 100 U/mL and a limit of detection (LOD) of 0.54 nU/mL (S/N = 3). The constructed electrochemical immunosensor has good selectivity, repeatability, reproducibility, and stability. Furthermore, determination of CA19-9 in human serum samples is also realized.Keywords:electrochemically reduced graphene oxide;carbon nanotubes;methylene blue;CA19-9;immunosensor
研究领域:
生物化学
·
纳米技术
·
分析化学
·
医学诊断
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DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2020
·
IF 4.65
Yang Li,Junben Huang,Huimin Chen
Single crystals of Al 3 (IO 3 ) 9 ·(HIO 3 ) 6 ·18H 2 O with sizes up to 4 × 4 × 3 mm 3 were grown by the aqua-solution method at room temperature . It crystallizes in the noncentrosymmetric (NCS) hexagonal space group P 6 3 (No. 173) with unit cell parameters of a = 16.1149 (10) Å, c = 12.3886 (11) Å and Z = 2. Its structure features a 3D framework composed by the IO 3 − , HIO 3 and [Al(H 2 O) 6 ] 3+ units. It shows a powder second harmonic generation (SHG) response of about 3.5 × KDP at the particle size of 55–88 μm. The thermal stability , IR and UV–Vis–NIR diffuse reflectance spectra of Al 3 (IO 3 ) 9 ·(HIO 3 ) 6 ·18H 2 O were studied comprehensively. In addition, the first-principles calculation was carried out in order to understand the electronic structure and the origin of the linear and nonlinear optical properties .
研究领域:
材料科学
·
无机化学
·
光学材料
·
晶体学
·
非线性光学
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DOI
高影响因子
Materials for Renewable and Sustainable Energy
·
2018
·Vol. 8
·期 1
·1-9
·
IF 0
Jiang L.,Zou Z. W.,Pei Q. M.,Zheng D. S.,Li F. S.,Tian Y. H.
Re 2 Mg(Ni 0.7 − x Co 0.2 Mn 0.1 Al x ) 9 ( x = 0‒0.04) alloys are prepared by induction melting, and the influence of the partial substitution of Ni by Al on the structure, hydrogen storage, and electrochemical properties of the alloys are investigated systematically. These alloys mainly consist of two main phases with LaNi 5 phase and (La,Mg) 2 Ni 7 phase, and minor LaNi 2 phase. The pressure-composition isotherms shows that, with Al content increasing in the alloys, the maximum hydrogen storage capacity decreased from 1.16 wt% ( x = 0) to 0.99 wt% ( x = 0.04). The changes of enthalpy and entropy reveal that the thermodynamic stability and the disordered degree of the hydride alloys increase with the Al addition. Results of electrochemical studies indicate that the substitution of Al for Ni can noticeably improve the cycle stability of the alloy electrode. The capacity retention after 80 cycles is enhanced from 63.6% ( x = 0) to 76.5% ( x = 0.04). However, the maximum discharge capacity of the alloys decreases. The Re 2 Mg(Ni 0.7 − x Co 0.2 Mn 0.1 Al x ) 9 ( x = 0‒0.04) alloys exhibit excellent dischargeability.
研究领域:
材料科学
·
氢能源研究
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产品关联
DOI
AICHE JOURNAL
·
2025
·
IF 4
Xinrui Liu,De Ao,Mao Ye,Zhi Wang,Zhihua Qiao,Song Zhao
Metal–organic framework (MOF) glass holds great promise for gas separation applications. However, the inherent low porosity of MOF glass poses a considerable challenge in improving membrane permeability. In this work, a self-supporting MOF crystal-glass composite (CGC) membrane was synthesized via an innovative blending and melting approach, in which ZIF-62 powder was converted into an amorphous glassy phase and ZIF-9 powder was partially flux-melted, effectively preventing the reorganization of Zn─N bonds within ZIF-62. Consequently, the composite component structure significantly enhances the porosity and permeability of the MOF glass membrane. Furthermore, the incorporation of cobalt into the membrane matrix has notably enhanced its adsorption affinity for CO 2 , thereby boosting CO 2 /CH 4 selectivity. The resultant a g [(ZIF-9) 0.2 /(ZIF-62) 0.8 ] membrane has demonstrated exceptional CO 2 permeability of 20,324 barrer and superior CO 2 /CH 4 selectivity, both surpassing the 2018 upper bound. Thus, the innovative strategy for the design of MOF CGC membranes offers effective solutions to the challenges inherent in gas separation processes.
研究领域:
材料科学
·
环境科学
·
化学工程
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Wanchuan Ding,Ge Zhang,Hui Zhang,Jingkun Xu,Yangping Wen,Jie Zhang
Poly(9-fluorenecarboxylic acid) (PFCA) thin film was successfully fabricated onto the surface of indium tin oxide glass by one-step electropolymerization of commercially available 9-fluorenecarboxylic acid in boron trifluoride diethyl etherate. Meanwhile, PFCA thin film could specifically recognize Fe 3+ . Fe 3+ was generated by the oxidization of Fe 2+ in the presence of H 2 O 2 that was produced by biochemical reactions and resulted in fluorescence quenching of PFCA thin film. Furthermore, a sensing platform was designed for highly selective fluorescent detection of glucose. The qualitative and quantitative analysis of Fe 3+ , H 2 O 2 and glucose with detection limits of 1 μM, 2.9 μM and 3.4 μM respectively could be implemented by a simple, cost-effective, reusable, practical and multi-functional PFCA thin film. The fabricated PFCA-based chemo/bio sensing film for fluorescent detection of Fe 3+ in rice sample or glucose in serum sample was assessed. Satisfactory results indicate that this work will provide not only a new strategy for fluorescent detection of important substances in agricultural science, but also significant theoretical guidance and important reference value for the practical application and development of multi-functional sensing device.
研究领域:
分析化学
·
生物传感
·
材料科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）