MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Ruimeng Sun,Ruijuan Lv,Ting Du,Yuhan Li,Yang Zhang,Lixia Chen,Yanfei Qi
Multi-enzymatic cascade reaction for analyte has the advantage of volume-confined environment and high-performance. Herein, a freeze–thaw induced co-assembly (FTIA) of multi-enzyme-AuNPs hybrids for high performance cascade reaction was developed for the first time. This preparation method does not require extra reagents and complex coupling steps, and has the superiority of simplicity, rapidity, low cost and environmental friendliness . Given the close proximity of glucose oxidase (GOx) and horseradish peroxidase (HRP) on the surface of AuNPs , this new detector greatly reduced the diffusion and decomposition of H 2 O 2 and improved the sensitivity of glucose detection. Hence, the acquired AuNPs/GOx/HRP can undoubtedly be used to construct a sensitive and speedy colorimetric sensor for the assay of glucose (linear range of 42–2670 μM with an LOD of 10 μM). In addition, it was proved that AuNPs can be used as versatile scaffolds by simply replacing GOx with xanthine oxidase (XOD) to detect hypoxanthine (linear range of 20–1330 μM with an LOD of 20 μM). The constructed glucose detection method can be completed in 10 min and has been applied to the assay of glucose in human blood samples.
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高影响因子
ELECTROANALYSIS
·
2023
·Vol. 35
·期 9
·e202300064
·
IF 3
Jinjin Zhang,Wenjing Liu,Qi Wang,Jianying Qu
In this work, two-dimensional cobalt metal organic framework nanosheets ( CoTP ) were prepared by the simple hydrothermal reaction of cobalt nitrate hexahydrate with terephthalic acid, and then calcined at a high temperature under the environment of nitrogen to obtain cobalt carbon nanomaterials ( Co-CNs ). The gold nanomaterials ( AuNPs ) were prepared by redox reaction. The prepared materials were characterized and analyzed by XRD, SEM and IR. A novel electrochemical sensor ( AuNPs/Co-CNs ) was constructed successfully on a glassy carbon electrode combining Co-CNs with AuNPs , which showed significant electrocatalytic activities for acetaminophen ( PA ) and 4-aminophenol ( 4-AP ) and could be used to detect them simultaneously. Under the optimal conditions, the linear ranges for determination of PA and 4-AP were 0.50–250.00 μM and 0.50–350.00 μM with the detection limits of 0.11 μM and 0.10 μM, respectively. Compared with other related reports, the proposed sensor exhibits a wider linear ranges, good sensitivity, accuracy and selectivity with potential applications in real sample analysis.
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Nanoscience and Nanotechnology Letters
·
2019
·Vol. 11
·期 5
·638-644
·
IF 0
Jiang Pengfei,Haji Chomba,Ye Xiufang,Chang Menglei,Li Wenjun,He Xuliang,Chen Dongchu,Nie Libo
An inductive coupled plasma mass spectrometry (ICP-MS) method based on gold nanoparticles (AuNPs) and bio-barcode signal amplification was presented for the sensitive detection of gastric cancer related gene. The target DNA, magnetic nanoprobes and SiO2/AuNPs barcode probes
were hybridized to form a "sandwich" structure, then a large amount of AuNPs which were amplified by bio-barcode technology were detected by ICP-MS. This method presented a limit of detection as low as 1 fM. The ratio of the background-subtracted 197 Au signals for totally complementary
DNA, single-base mismatched DNA, double-base mismatched DNA and totally mismatched DNA was 115:30:16:1, respectively. It's suggested that the complementary and mismatched DNA can be distinguished clearly. This novel ICP-MS biosensor is prospective in DNA detection with high sensitivity and
specificity.
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 8
·1-11
·
IF 5.833
Zhang Youxiong,Li Bing,Wei Xianhu,Gu Qihui,Chen Moutong,Zhang Jumei,Mo Shuping,Wang Juan,Xue Liang,Ding Yu,Wu Qingping
A facile and versatile competitive electrochemical aptasensor for tobramycin (TOB) detection is described using electrochemical-deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework (AuNPs/P-MOF) as signal-amplification platform and a DNA probe labeled with methylene blue (MB) at the 3′-end (MB-Probe) as a signal producer. First, F-Probe (short complementary DNA strands of both the aptamer and the MB-Probe label with a sulfhydryl group at the 5′-end) was immobilized on the AuNPs/P-MOF modified electrode as detection probes, which competed with TOB in binding to the aptamer. TOB-aptamer binding resulted in F-Probe remaining unhybridized on the electrode surface, so that a significant current response was generated by hybridizing with MB-Probe instead. The developed strategy showed favorable repeatability, with a relative standard deviation (RSD) of 4.3% computed over five independent assays, and high stability, with only 6.8% degradation after 15 days of storage. Under optimal conditions, the proposed aptamer strategy exhibited a linear detection range from 100 pM to 500 nM with a limit of detection (LOD) of 56 pM (S/ N = 3). The electrochemical aptasensor demonstrated remarkable selectivity, and its feasibility for accurate and quantitative detection of TOB in milk samples was confirmed (RSD < 4.5%). Due to its simple design, easy operation, and high sensitivity and selectivity, the proposed method could expect to detect other antibiotics by replacing the aptamers. In summary, this study provides a simple and effective new strategy for electrochemical aptasening based on MOF-based sensing interface. Graphical abstract
Scheme illustration of label-free competitive electrochemical aptamer-based detection of tobramycin based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework as signal-amplification platform.
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高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 6
·B524
·
IF 3.12
Yingchun Li,Zedong Li,Hao Liu,Siyue Chen,Xiaojin Guo,Min Lin,Fei Li
Point-of-care testing (POCT) of neurotransmitters, such as dopamine (DA) and 5-hydroxytryptamine (5-HT), can be used for early diagnosis of neurological diseases. Among various POCT platforms, electrochemical method-based platforms have attracted increasing interest owning to their high detection sensitivity and specificity as well as fast response time. In this work, we developed a portable electrochemical POCT platform for detection of neurotransmitters, which is based on integration of a three-dimensional (3D) gold nanoparticles/carbon nanotubes (AuNPs/CNTs) sponge synthesized by a simple in-situ growth and eco-friendly ice-templating method modified screen-printed electrode with a miniaturized USB electrochemical analyzer. We demonstrated the good performance of the portable electrochemical platform for detection of two typical neurotransmitters, i.e., DA and 5-HT, with detection sensitivities of 1.02 μA μM−1 cm−2 and 0.55 μA μM−1 cm−2, and detection limits of 0.06 μM and 0.30 μM for DA and 5-HT, respectively. We further verified the feasibility and practicability of the platform by simultaneous detection of DA and 5-HT in spiked saliva. The developed portable electrochemical platform provides a simple and user-friendly way for early diagnosis of neurological diseases in the future, especially at point of care.
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Biosensors-Basel
·
2026
·Vol. 16
·期 7
·388
·
IF 6.2
Yaning Liang,Shiyi Fang,Zhuoer Chen,Yuzhuo Chen,Qingqing Yang,Xuelan Shu,Tao Le
Gold nanoparticles (AuNPs) are widely used in aptasensors because of their high extinction coefficient and aggregation-dependent color differences. However, recent studies have indicated that nonspecific interactions between target molecules and AuNPs may dominate the detection signal rather than aptamer–target specific binding. This study systematically investigated the interactions between 13 aminoglycoside antibiotics and AuNPs. We found that all aminoglycoside antibiotics interacted strongly with AuNPs, considerably reducing their salt stability. Furthermore, methoxy polyethylene glycol thiol reversed AuNP aggregation induced by aminoglycoside antibiotics, indicating that it occurs at the secondary minimum. Using density functional theory, we analyzed the molecular structures and charge distribution characteristics of the aminoglycoside antibiotics, elucidating that they replace citrate ions on AuNP surfaces via a ligand exchange mechanism, thereby inducing aggregation. Additionally, both aptamer targets and complementary DNA struggled to desorb the aptamer (KAN6-1) from the AuNP surfaces. Our study demonstrates that the label-free colorimetric assay based on aggregation of unmodified citrate–AuNPs is neither suitable for characterizing the binding affinity of aminoglycoside aptamers nor viable for constructing corresponding colorimetric sensors to detect this class of antibiotics. Thus, researchers should incorporate mechanistic verification and rigorous controls when employing this system to ensure reliable results.
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高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Zihan Shi,Dingqiang Lu,Wei Xu,Lihua Geng,Chang-jiang-sheng Lai
Developed an electrochemical biosensor for the bitter taste receptor TAS2R1, utilizing a nanocomposite of CMK-3 and AuNPs. • Proposed system-dependent C₁/₂,sys to quantify ligand-triggered electrochemical signals of the TAS2R1 biosensor. • H-bond & hydrophobic forces govern TAS2R1-ligand binding; Phe75, Ile254, Leu250, Phe252/259, Arg256 are key binding residues. The bitter taste receptor TAS2R1 mediates significant physiological functions not only in the gustatory system but also in extra-oral tissues, including the respiratory tract, immune system, and metabolic pathways. To elucidate its ligand recognition mechanism, we developed an electrochemical biosensor for TAS2R1 based on a composite of ordered mesoporous carbon and gold nanoparticles. The biosensor was used to quantitatively analyze the interaction kinetics with ten bitter ligands. Combined with molecular docking and molecular dynamics simulations, this study identified key residues in TAS2R1 that may be involved in ligand recognition; among these, the nitrogen-containing groups and hydroxyl groups of the ligand molecules appear to be important structural features influencing ligand recognition by TAS2R1. This work provides a molecular foundation for elucidating the structure-activity relationship of TAS2R1 and facilitates its applications in bitter taste masking and drug development. Download: Download high-res image (174KB) Download: Download full-size image
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 7
·495
·
IF 5.4
Shi Jian,Shan Xiaomu,Wang Hongren,Xu Yuanze,Zhang Zezheng,Fan Xiuwei,Du Xuejian,Jiang Shouzhen
A high-performance optical fiber surface plasmon resonance (SPR) biosensor for the determination of dopamine (DP) is reported based on gold nanocone arrays combined with gold nanoparticles (AuNPs). The sensor was fabricated via thermal nanoimprinting, gold sputtering, and single-stranded DNA-assisted AuNPs immobilization to form gap hotspots. Finite element simulation and experimental results verified strong electromagnetic field enhancement via extraordinary optical transmission and SPR-LSPR coupling. The sensor achieved a detection limit of 2.7 × 10 -14 M for DA, with sensitivity and FOM improved by 37.4% and 54.2% relative to conventional gold film structures. It showed excellent selectivity and stability in serum, cerebrospinal fluid (CSF), and whole blood. This robust, scalable design provides a promising label-free platform for ultrasensitive DA detection in clinical point-of-care applications. Graphical abstract
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高影响因子
Foods
·
2026
·Vol. 15
·期 7
·1164
·
IF 6
Tahirou Sogore,Meimei Guo,Jin Huang,Xinyu Liao,Tian Ding,Mofei Shen
Foodborne pathogens cause hundreds of millions of illnesses annually, underscoring the urgent need for advanced antimicrobial food packaging materials. The objective of this study was to develop a crosslinked cyclodextrin metal–organic framework, loaded with gold nanoparticles (CL-AuNPs@CD-MOF) and integrated into a PLA-Zein composite film with humidity-responsive antimicrobial release, as a sustainable and high-performance packaging solution to address the critical limitations of conventional materials in controlling microbial contamination during food storage. Therefore, gold nanoparticles (AuNPs) were synthesized via a green approach using CD-MOFs as stabilizers and p-coumaric acid as a natural reducing agent, then crosslinked with diphenyl carbonate (DPC) to produce CL-AuNPs@CD-MOF. Crosslinking conditions were optimized to a CD-MOF:DPC ratio of 1:1, 1080 min reaction time, and 80 °C, preserving the cubic morphology and crystalline structure while transforming burst release into sustained antimicrobial activity againstE. coliandS. aureusover 7 days. Then, the incorporation of CL-AuNPs@CD-MOF into PLA-Zein films yielded a composite packaging material with favorable mechanical and barrier properties, including a water vapor transmission rate of 539.44 g/m2·24 h and an oxygen permeability of 235.90 cm3/m2·24 h·0.1 MPa. Progressive elimination ofE. coli,S. aureus, andL. monocytogenesover 7 days was confirmed, with antimicrobial efficacy originating exclusively from the CL-AuNPs@CD-MOF component. Application onAgaricus bisporusover 12 days of refrigerated storage demonstrated superior preservation performance: mushrooms inoculated withL. monocytogenesand packaged with CL-AuNPs@CD-MOF/PLA-Zein exhibited a weight loss of only 6.20 ± 2.06%, compared to 17.74 ± 3.15% for PLA-Zein and 41.50 ± 3.01% for PE controls. Color stability was equally improved, with lightness values of 71.46 ± 1.47 retained under CL-AuNPs@CD-MOF/PLA-Zein packaging, versus 58.37 ± 0.86 for PLA-Zein and 23.34 ± 2.34 for PE. Mushrooms inoculated withE. coliandS. aureusfollowed consistent trends. These results establish CL-AuNPs@CD-MOF/PLA-Zein as a promising multifunctional antimicrobial packaging platform for sustainable food preservation.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 4
·272
·
IF 5.4
Yang Dong,Li Xiangyu,Wu Jing,Yao Tuanli,Chen Chaoxian,Li Xuhua,Chen Jifan,Lan Xueyun,Wang Jiao,Wang Haihua
Semicarbazide (SEM), a metabolite of nitrofurazone, poses potential risks as a carcinogen and teratogen in animal-derived foods. This research is centered on creating a homemade electrochemical sensor with high sensitivity for detecting SEM with dual signals. The working electrode was modified through the in-situ growth of a copper-iron bimetallic organic framework (Cu/Fe-BTC) on carbon cloth (CC), followed by the decoration of gold nanoparticles (AuNPs) on the Cu/Fe-BTC/CC surface. The Cu/Fe-BTC component provides abundant metallic active sites, resulting in a distinct [Fe(CN) 6 ] 3−/4− peak observed at 0.67 V (I 1 , vs. SCE). Concurrently, the incorporation of AuNPs enhances the electrode high electron transfer capacity, which amplifies the signal peaked at 0.18 V (I 2 , vs. SCE), thereby establishing an effective dual-signal detection system. Furthermore, a molecularly imprinted polymer (MIP) layer was electropolymerized onto the modified electrodes, utilizing resorcinol as the functional monomer and SEM as the template. Then, the peak intensities of the dual signals decreased upon target (SEM) recognition and increased during template elution, generating a measurable detection signal. By calculating the current changes, ΔI D = ΔI 1 + ΔI 2 , the adsorbed template molecule concentration could be quantified. Under optimal conditions, the sensor has excellent stability, accuracy, and repeatability in analyzing various samples, including serum, urine, meat, egg, honey, canned food, flour, milk powder, fish, and shrimp. The determination range is linear, spanning from 0.005 to 100 nM, with a limit of detection at 3.9 pM. Therefore, the Cu/Fe-BTC and AuNPs-based MIP sensor represents a reliable dual-signal strategy for the quantitative determination of SEM. Graphical
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高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 5
·321
·
IF 5.4
Liu Shicong,Zhang Yuanyuan,Zhao Hongyang,Liu Junqiu,Hou Chunxi
A “turn-on” fluorescence sensor was developed for cholesterol detection on the basis of fluorescence resonance energy transfer (FRET) between the organic fluorophore 1-(4-(1,2,2-triphenylvinyl)phenyl)pyrene (TPE-Py) and a gold nanoparticle-β-cyclodextrin conjugate (AuNPs-β-CD). Mechanistically, the AuNPs-β-CD, an energy acceptor, was synthesized via Au–S bonding, and TPE-Py transferred energy to the AuNPs via FRET, resulting in fluorescence quenching of TPE-Py. In the presence of cholesterol, which has a stronger binding affinity for the β-CD cavity, TPE-Py was competitively displaced, enabling the recovery of the fluorescence signal. This sensor has high sensitivity for cholesterol, with a low detection limit of 66 nM and a linear range of 0.5–30 µM. Furthermore, the sensor was successfully used to determine the cholesterol content in food samples with satisfactory recoveries, revealing its potential for food analysis.
Section
Graphical abstract
The alternative text for this image may have been generated using AI.
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高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2026
·
IF 4.6
Zhitan Huang,Tao Liu,Jiaqi Geng,Siyue Zhang,Neha,Miantai Ye,Wei Sun
Kanamycin (KANA), a broad-spectrum antibiotic, is a potential threat to the human health and environment. Therefore, it is of great significance to establish a highly sensitive and selective detection method for KANA. A novel molecularly imprinted polymers (MIPs) electrochemical sensor based on Au nanoparticles (AuNPs), nitrogen-doped graphene (NG) and MXene nanocomposite modified glassy carbon electrode (MIPs/AuNPs-MXene-NG/GCE) was developed for KANA analysis in this paper. The physicochemical performances of the nanocomposite were characterized using various techniques including SEM, TEM, XRD, XPS and electrochemsitry. The results showed that the decoration of AuNPs on MXene enhanced the conductivity and electrochemical response, the presence of MXene and NG increased the specific surface area and active sites, and the synergistic effects were favorable to the electrode performances. Under the optimal conditions, MIPs/AuNPs-MXene-NG/GCE was applied to KANA detection using differential pulse voltammetry (DPV), which achieved a wide detection range from 0.1 nmol/L to 1.0 mmol/L with a low detection limit of 0.033 nmol/L (S/N = 3). Moreover, the sensor shows excellent stability, selectivity and reproducibility, and successfully applied to the detection of KANA content in different types of milk samples with satisfactory results. The recovery was ranged from 86.0% to 113.0% with the relative standard deviation less than 4.06%. Therefore, the developed sensor provides a new idea for the efficient analysis of KANA in food samples.
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