INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Shuoshuo Yang,Guoqi Ni,Mengyang Xia,Hailing Li,Zhonghong Gao
Oxidative stress is associated with most traumatic or pathological bone defects, and seriously affects the effect of implantation. The construction of antioxidative and osteogenic coatings is of great significance to accelerate the bone regeneration of implants. In this study, baicalein (BAI), a nature flavonoid drug, was loaded in bovine serum albumin (BSA) by desolvent method to prepare BAI-BSA composite protein, and tannic acid (TA)/BAI-BSA coatings were further built via layer by layer self-assembly technology. BAI-BSA possesses good biocompatibility that showed no cytotoxicity to osteoblasts and erythrocytes, and helps to enhance the activity of alkaline phosphatase (ALP) and promote the formation of osteogenic mineralized calcium nodules. After assembled with TA, BAI-BSA coating significantly promoted cell adhesion and in vitro osteogenic mineralization of MC3T3-E1. Moreover, BAI drug loading improved the antioxidative function of BSA coatings effectively. The scavenging rates of (TA/BAI-BSA-10) 4 for ABTS +• and DPPH • free radicals were 69.6 ± 16.1 % and 53.4 ± 2.4 %, respectively. At cellular level, the TA/BAI-BSA coating effectively inhibited the impact of oxidative stress on the oxidative damage of osteoblasts. The drug-loaded protein coatings possess both great antioxidative and osteogenic functions, which have important potential in the field of bone repair.
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高影响因子
DESALINATION
·
2020
·
IF 7.098
Fuqing Ma,Chaojie Cai,Xinzhen Zhao,Hilal Ahmad,Changkun Liu
A novel thin-film nanocomposite (TFN) forward osmosis (FO) membrane was fabricated by loading bovine serum albumin (BSA)/gold nanoparticles (GNPs) on the surface of a cockscomb-like nylon supporting membrane. The influence of the embedded BSA/GNPs on surface morphology , surface roughness , surface charge, separation, and FO performance of the TFN-FO membrane was investigated. The water flux of the BSA/GNPs-modified FO membrane could be increased to 55.2 ± 5.4 LMH, and the J s /J w value was substantially decreased to 0.034 g/L, indicating a high selectivity of the modified membrane. BSA/GNPs located between the top of the nylon supporting membrane and the bottom of the polyamide (PA) skin layer provided passage for water transport and increased the roughness of the PA layer, thus enhancing the effective area on which the osmotic pressure could act. In addition, BSA/GNPs located at the bottom surface of the nylon supporting membrane provided a nearly electroneutral surface in the solution and alleviated dilutive concentration polarization . In summary, choosing a nylon membrane with a special cockscomb-like surface structure as the supporting layer to load BSA/GNPs nanoparticles proved to be a creative and effective nano-modification method for fabricating high-performance TFN-FO membranes.
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2018
·
IF 3.997
Jing Zhang,Biao Shen,Liqun Chen,Lingdong Chen,Yangsheng Meng,Jie Feng
A multifunctional envelope-type mesoporous silica nanoparticle (MSN) was delicately designed for subcellular co-delivery of drug and therapeutic peptide to tumor cells. Firstly, a kind of cell apoptosis peptide (KLAKLAK) 2 (KLA) was anchored on surface of MSN via disulfide bond to obtain MSN-SS-KLA. Subsequently, anticancer drug doxorubicin hydrochloride (DOX) was loaded into the pores of MSN-SS-KLA. Then, the drug loaded MSN-SS-KLA (DOX@MSN-SS-KLA) was further coated with bovine serum albumin (BSA) to obtain a biological media stable MSN based drug delivery system (DDS), DOX@MSN-SS-KLA/BSA, for cancer synergetic therapy. The results show that stability of the DOX@MSN-SS-KLA/BSA is much better than that of DOX@MSN-SS-KLA and it could keep well dispersed in serum for more than 24 h. After accumulating at tumor site by EPR effect, the DOX@MSN-SS-KLA/BSA could be effectively phagocytosed by HeLa cells and release apoptotic peptide KLA as well as DOX simultaneously responding to reductive stimulus inside the cells. In vitro cell experiment results show that the DOX@MSN-SS-KLA/BSA complex exhibits much better inhibition on HeLa cells compared with pure DOX, indicating that co-delivery of KLA and DOX is expected to achieve synergetic therapy of cancer.
研究领域:
纳米技术
·
药学
·
癌症研究
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生物医学工程
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高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Yimu Qiao,Xue Han,Feiyong Chen,Cuizhen Sun,Linxu Xu,Rupeng Liu,Xue Shen
To guide the practical application of ozonation and adsorption coupled with ceramic membrane technology , the mitigation mechanisms of O 3 pre-oxidation, powdered activated carbon (PAC) pre-adsorption, and combined O 3 -PAC pretreatment on membrane fouling caused by natural organic matter (NOM) were investigated. The membrane and water quality were characterized by scanning electron microscopy (SEM), energy dispersive spectrometer (EDS), three-dimensional fluorescence-parallel factor analysis (PARAFAC-EEM), dissolved organic carbon (DOC), laser particle size and Zeta potential . Results showed that the membrane flux decline caused by humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and surface water were alleviated by 35.2%, 55.2%, 31.0% and 59.0% after O 3 -PAC pretreatment. Meanwhile the removal rates of HA, SA and BSA were 26.2, 74.8% and 42.3%, respectively. The combination of PAC and O 3 can effectively control the membrane fouling caused by HA and SA. Ozonation can degrade organic matter into small molecules, and PAC has high adsorption to these small molecules, by recycling PAC can take away this part of organic matter, thereby reducing membrane fouling. O 3 showed no obvious advantage for membrane fouling caused by BSA, and the decrease of membrane fouling was mainly attributed to the adsorption of PAC. This investigation highlights the potential mechanism of O 3 -PAC in alleviating the membrane fouling caused by different organic compounds, contributing to the efficient and low-carbon operation of the production.
研究领域:
环境工程
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水处理
·
膜技术
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2013
·Vol. 37
·期 7
·2174-2178
·
IF 2.966
Xing Liu,Qi Wang,Yu Zhang,Lichun Zhang,Yingying Su,Yi Lv
In this paper, a novel and simple colorimetric method for the determination of glutathione (GSH) was developed. The BSA–MnO2 NPs possess oxidase-like activity that can catalyze the oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB), and the existence of GSH can cause reduction of oxidized TMB along with a visual color variation. A good linear relationship can be obtained from 0.26 to 26 μM with a limit of detection of 0.1 μM. Furthermore, the good recoveries for serum samples indicated that the present colorimetric methodology was feasible, simple and sensitive, with a promising application in routine analysis of biosamples.
研究领域:
分析化学
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生物化学
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纳米技术
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高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2015
·Vol. 55
·期 12
·2829-2837
·
IF 1.52
Shushan Yuan,Juan Wang,Xiaojun Wang,Shengru Long,Gang Zhang,Jie Yang
Poly(arylene sulfide sulfone) (PASS) is a kind of newly developed polymeric membrane material which has excellent mechanical strength, thermal stability, and solvent resistance. And, it would be a potential material for high temperature ultrafiltration and organic solvent filtration. In this article, PASS hybrid ultrafiltration membrane with improved antifouling property was prepared by mixing TiO 2 nanoparticles which were grafted with polyacrylic acid (PAA). These membranes were prepared by a phase inversion technique and their separation performance and antifouling property of the prepared membranes were investigated in detail by SEM, FTIR, EDS, contact angle goniometry, filtration experiments of water, and BSA solution. The results shown that the TiO 2 - g -PAA nanoparticles dispersed well in membrane matrix, the hydrophilicity of the membranes prepared within TiO 2 - g -PAA nanoparticles have been improved and these membranes exhibited excellent water flux and antifouling performance in separation than that of the pure PASS membranes and PASS membranes with TiO 2 nanoparticles. More specifically, among membrane sample M0, M1.5, and MP1.5, MP1.5 which contained 1.5 wt% TiO 2 - g -PAA exhibited the highest water permeation (190.4 L/m 2 h at 100 kPa), flux recovery ratio, and the lowest BSA adsorption amount. POLYM. ENG. SCI., 55:2829–2837, 2015. © 2015 Society of Plastics Engineers
研究领域:
材料科学
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化学工程
·
膜技术
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高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2024
·
IF 8.1
Chunru Sun,Min Xiao,Jianghao Tian,Huixian Zhang
A polysulfone/metal–organic framework (MOF) based photocatalytic nanofiltration membrane is proposed in this study. Interfacial polymerization (IP) is the universal method of preparing nanofiltration (NF) membranes. In this work, the (TFN) membranes were synthesized with an in-situ interfacial synthetic method to incorporate BiOCl/MIL-101(Fe)@PDA(BMP) into the polyamide (PA) selective layer. Five distinct BMP loadings (0, 0.05, 0.1, 0.15, and 0.2 wt%) were established to examine the impact of additional BMP on the physicochemical characteristics of the produced TFN membranes. Under the irradiation of a xenon lamp with a power of 300 W, the water flux of M3 at a pressure of 0.6 MPa was 277.14 L m -2 h -1 . Furthermore, the BSA-based antifouling tests demonstrated the membrane’s outstanding antifouling capabilities, with a flux recovery rate reaching 94.08 %. The optimal membrane maintained a high and stable retention of tetracycline for 12 h. The interfacial synthesis of MOF/PA offers a new approach to the fabrication of photocatalytic NF membranes.
研究领域:
材料科学
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环境工程
·
化学工程
·
纳米技术
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高影响因子
POLYMER BULLETIN
·
2017
·Vol. 75
·期 6
·2627-2639
·
IF 1.43
Cao Qi,He Naipu,Wang Yue,Lu Zhenwu
Abstract Amphiphilic globular protein–polymer hybrids, consisting of poly(methyl methacrylate) (PMMA) as hydrophobic tail and globular protein as hydrophilic head, are prepared by the end-functional PMMA covalently binding to the primary amino groups of bovine serum albumin (BSA) in the phosphate buffer saline (PBS)/acetone mixture solvents at pH 5. The self-assembly behaviors of amphiphilic BSA–PMMA hybrids are explored in aqueous solution by the dynamic light scatter (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The self-assembled morphologies of the amphiphilic BSA–PMMA hybrids are controlled by the length of hydrophobic PMMA chains. The amphiphilic BSA–PMMA hybrids with the longer hydrophobic PMMA chains self-assembled into spherical vesicles and elongated tubular vesicles, and conversely, they self-assembled into micelles. A vesicle consists of BSA as two outer hydrophilic layers and PMMA as hydrophobic wall. Graphical Abstract Amphiphilic globular protein–polymer hybrids consist of PMMA as hydrophobic tail and globular protein BSA as hydrophilic head. Amphiphilic globular protein–polymer hybrids self-assembled into micelles or vesicles in phosphate buffer saline. The self-assembled morphology of amphiphilic protein–polymer hybrids was controlled by adjusting the length of hydrophobic chains.
研究领域:
材料科学
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聚合物化学
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纳米技术
·
胶体科学
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高影响因子
Journal of Materials Chemistry B
·
2015
·Vol. 4
·期 4
·743-751
·
IF 4.726
Ya Wen,Haiqing Dong,Yan Li,Aijun Shen,Yongyong Li
Biomineralization of a rare earth ion (Gd) is first employed to assemble bovine serum albumin (BSA) into sub-50 nm nanoparticles (Gd@BSA) for theranostic applications, via a straightforward and reproducible strategy. Combination of Gd ions with BSA under mild conditions results in the formation of the Gd@BSA nanosystem, which has been used as the scaffold material to encapsulate a photosensitiser (Ce6) with high efficiency up to 20 wt%. Beyond playing an important role in the assembled process, the incorporation of Gd affords the potential MRI and fluorescence imaging capability of Gd@BSA-Ce6 nanoparticles. In vivo MRI allowed real-time imaging in tumor-bearing mice and showed advantages in terms of circulation time, compared with commercially used Gd–DTPA. Gd@BSA-Ce6 nanoparticles exhibited enhanced tumor-specific distribution, through enhanced permeability and retention effect, and complete cure of tumor-bearing mice after intravenous injection. The nanoparticles did not produce systemic toxicity as revealed by biodistribution and histology toxicity analyses. The results demonstrate the great potential of Gd@BSA-Ce6 nanoparticles as theranostic agents due to their excellent imaging and tumor-growth-inhibition properties.
研究领域:
纳米医学
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癌症治疗
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分子影像
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生物医学工程
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Yuhan Fu,Hui Han,Yaming Xu,Hongbo Cui,Xiang Yao,Guijian Guan,Ming-Yong Han
With large surface-responsive and excitation-dependent fluorescence, two-dimensional fluorescent quantum dots (QDs) have been receiving tremendous attention to develop their facile synthetic approaches and/or expand their promising applications. Here, a two-step strategy is demonstrated for high-yield production of MoS 2 QDs from MoS 2 powder through first sonication-driven exfoliation and subsequent hydrothermal splitting with the assistance of bovine serum albumin (BSA). Experimentally, ∼100 nm-sized MoS 2 nanosheets are ultrasonically exfoliated from MoS 2 powder in a BSA solution, and further hydrothermally split into ∼ 8.2 nm-sized QDs (NQDs) at 200 °C. In addition to their excellent stability/dispersibility in aqueous solution, the resultant MoS 2 NQDs also exhibit much brighter blue fluorescence than those synthesized by other methods. The strong fluorescence is significantly quenched by p-nitrophenol for constructing a sensitive sensor with high selectivity, which is attributed to dual quenching effects from inner filter effect (IFE) and fluorescence resonance energy transfer (FRET). Interestingly, with the increment of pH from 5 to 10, the ratio of IFE in fluorescence quenching gradually decreases accompanied by an increment of FRET ratio, resulting in the high sensitivity and responsivity for detecting p-nitrophenol at a wide range of pH. Clearly, the MoS 2 NQD-based sensing approach demonstrates a promising potential for selective detection and fast analysis of pollutants in environment monitoring and security screening.
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高影响因子
NANO
·
2024
·
IF 1
Zhenting Wu,Hang Song,Ruifang Liu,Chaoyi Yin,Long Ba
In recent years, graphene biosensors have received much attention due to their high sensitivity, low noise and low detection limit. Vertical graphene field effect transistors (VGFETs) based on graphene/semiconductor heterojunctions are characterized by high switching ratios and have wide application prospects. In this study, we prepared the ion-gel gate VGFETs and used them for bovine serum albumin (BSA) quantification sensors. The results showed that the adsorption of BSA molecules on the substrate surface was maximized when the ionic strength was 10 − 3 M. The limit of detection (LOD) of BSA concentration was 1 μ g/ml, suitable for simple and rapid quantitative analysis of protein molecules.
研究领域:
纳米技术
·
生物传感器
·
材料科学
·
生物电子学
·
分析化学
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高影响因子
FIBERS AND POLYMERS
·
2016
·Vol. 17
·期 11
·1820-1828
·
IF 1.022
Yang Xue,Liu Hui,Zhao Yanjiao,Liu Lifang
Cellulose nanocrystals (CNCs) extracted from corn husks were used as additive to modify the hydrophilicity and anti-fouling properties of polysulfone (PSf) membrane. The PSf/CNCs blend membranes were prepared via an immersion phase inversion method. The influence of CNCs content on the morphology, structure, and performances of PSf membrane were carefully investigated by SEM, TG, DTG, DSC, break strength, elongation-at-break, Young modulus, contact angle and filtration experiment. The results showed that the isolated CNCs from corn husks were a promising additive for modifying the properties of PSf membrane. CNCs can improve mechanical property, thermal stability, hydrophilicity and anti-fouling performance of the pure PSf membrane. The PSf/CNCs blend membrane reached optimal properties at 2 wt% CNCs content, which was 2.76 and 1.57 times in pure water flux and FRR values respectively as compared to pure PSf membrane. Meanwhile, PSf-2 also can maintain a relatively high BSA rejection.
研究领域:
材料科学
·
纳米技术
·
膜技术
·
环境工程
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高影响因子
危险品化学品经营许可证（带存储）