SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Bang Lin Li,Jun Hua Luo,Hong Qun Luo,Nian Bing Li
A novel electrochemical sensor based on d -penicillamine ( d -PA) molecularly imprinted polypyrrole film on a glassy carbon electrode was fabricated. The complementary cavities located on the surface of molecularly imprinted polypyrrole film were used for subsequent template recognition. It was confirmed that the molecularly imprinted polypyrrole modified glassy carbon electrode (MIP-GCE) showed greater recognition ability than the non-molecularly imprinted polypyrrole modified glassy carbon electrode (NIP-GCE). Considering the electrochemical oxidation of d -PA with ferrocyanide and the specific recognition of molecular imprinting technique, the highly selective determination of d -penicillamine was achieved on the d -PA molecularly imprinted polypyrrole modified glassy carbon electrodes using ferrocyanide as an electrochemical probe. Several factors affecting sensor response, such as film thickness, template concentration, and the pH of phosphate buffer solution, were investigated to achieve optimum electrochemical response to d -PA at the MIP-GCE. The MIP-GCE revealed highly linear behavior in the concentration range of 10.0–480.0 μmol L −1 with the detection limit of 3.5 μmol L −1 for d -PA. The novel sensor has been applied to the determination of d -PA in real biological samples with satisfactory results.
研究领域:
分析化学
·
生物医学工程
·
电化学
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DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 15
·2645
·
IF 5.1
Cong Zhang,Kai Jiang,Yilin Lin,Rui Cui,Hong Wu
In this study, a composite film with dual antioxidant and antibacterial properties was prepared by combining 2% chitosan and 7% gelatin (2:1, w:w), with D-carvone (0–4%) as the primary active component. The effect of D-carvone content on the performance of the composite films was systematically investigated. The results showed that adding 1% D-carvone increased the water contact angle by 28%, increased the elongation at break by 35%, and decreased the WVTR by 18%. FTIR and SEM confirmed that ≤2% D-carvone uniformly bonded with the substrate through hydrogen bonds, and the film was dense and non-porous. In addition, the DPPH scavenging rate of the 1–2% D-carvone composite film increased to about 30–40%, and the ABTS+scavenging rate increased to about 35–40%; the antibacterial effect on Escherichia coli and Staphylococcus aureus increased by more than 70%. However, when the addition amount was too high (exceeding 2%), the composite film became agglomerated, microporous, and phase-separated, affecting the film performance, and due to its own taste, it reduced the sensory quality of the noodles. Comprehensively, the composites showed better performance when the content of D-carvone was 1–2% and also the best effect for freshness preservation in Xinjiang ramen. This study provides a broad application prospect for natural terpene compound-based composite films in the field of high-moisture, multi-fat food preservation, and provides a theoretical basis and practical guidance for the development of efficient and safe food packaging materials. In the future, the composite film can be further optimized, and the effect of flavor can be further explored to meet the needs of different food preservation methods.
研究领域:
食品科学
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高分子科学
·
材料工程
·
食品包装
·
抗菌技术
·
抗氧化研究
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产品关联
DOI
PubMed
高影响因子
Journal of the American Chemical Society
·
2025
·
IF 15.6
Mingyan Chuai,Hao Tong,Zimin Yang,Siting Deng,Mingqiang Wu,Jidan Xing,Guoliang Chai
An electrolytic Zn–MnO2 battery is highly valued due to its cost-effectiveness, environmental friendliness, and abundant resource availability. However, the battery’s performance is hindered by the slow kinetics at the poorly conductive MnO2 cathode and hydrogen evolution at the Zn anode. Here, a strategy of P–O–M (M = Mn, Zn) d-pπ backbonding design is proposed for phosphorus–oxygen electrolyte additives, which can be realized by tuning the atomic dipole moment-corrected Hirshfeld (ADCH) population charge of the P/O atom. The reversibility of d-pπ backbonding not only leads to the fast kinetics of Mn2+/Zn2+ at electrodes during both charge and discharge processes to suppress the competitive hydrogen evolution reaction but also enhances the electronic conductivity at the electrode–electrolyte interfaces to sustain the high areal capacity of batteries. Hydroxymethyl dimethyl phosphite (HPD) with d-pπ backbonding is a preferred additive with a suitable ADCH charge. The assembled electrolytic Zn–MnO2 (HPD) battery exhibits a high discharge capacity of 14.05 mAh cm–2 at an areal capacity of 15 mAh cm–2 and superior cycling stability over 1500 cycles. The Zn–MnO2 (HPD) soft-pack battery exhibits a discharge capacity of over 1.60 Ah at a discharge rate of 0.5 C and maintains a Coulombic efficiency of ∼80% over 100 cycles. Furthermore, the assembled 50 V 40 Ah commercial Zn–MnO2 (HPD) battery can drive an electric vehicle for 10 km. The ADCH charge regulation provides a feasible and effective method for developing high-performance aqueous batteries by achieving d-pπ backbonding.
研究领域:
电化学储能
·
材料科学
·
能源化学
·
应用化学
·
电池技术
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产品关联
DOI
PubMed
高影响因子
Systems Microbiology and Biomanufacturing
·
2022
·Vol. 2
·期 3
·507-522
·
IF 0
Du Juanjuan,Zhang Liang
N -methyl- d -aspartic acid (NMDA), an amino acid existing in human and animal central nervous system, exerts agonist action on one of the glutamate receptor subtypes and is clinically used for treatment of diabetes, Parkinson’s and Alzheimer’s syndrome. In this study, an enzymatic protocol for the chiral resolution of N -methyl- d , l -aspartic acid was built with a predicted N -demethylase (GenBank ID: OJV90073.1) from the genome of Chloroflexi bacterium 54-19. Through sequence alignment, the enzyme shares an identity of 32.19% to 2UZZ (PDB ID) with a conserved catalytic center. Recombinantly expressed in Bacillus subtilis WB600 , the N -demethylase was characterized with optimal temperature and pH at 55 ℃ and 7.5, and adaptive temperature and pH were 40–60 ℃ and 6–8. The effects of organic solvents and metal ions were investigated as well. Compared with other previously reported sarcosine oxidases, the enzyme showed a specific N -demethylation activity against N -methyl- l -aspartic acid according to the analysis by chiral liquid chromatography, LC–MS/MS and a detection by polarimeter. The results demonstrated 76% of 4.5 mM N -methyl- d , l -aspartic acid could be chirally separated by 7 mg·L −1 enzyme after reaction of 80 min. This work provided a foundation for mild synthesis of NMDA in industry. Graphical abstract
研究领域:
生物化学
·
分子生物学
·
微生物学
·
药理学
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DOI
JOURNAL OF APPLIED POLYMER SCIENCE
·
2012
·Vol. 129
·期 2
·767-772
·
IF 1.289
Dagen Zhong,Zonghua Liu,Shasha Xie,Wei Zhang,Yuanming Zhang,Wei Xue
This work is to develop a novel dual drug delivery system that can simultaneously load and release 18β-glycyrrhetinic acid (GA, a hydrophobic drug) and bovine serum albumin (BSA, hydrophilic model drug) in a single formulation. The system consists of poly( D,L -lactic) (PDLLA) microspheres embedded in calcium alginate hydrogel beads. The GA-loaded microspheres were first prepared and then dispersed in the aqueous solution of sodium alginate and BSA. The resulting suspension was dropped into aqueous calcium chloride solution to obtain the dual drug delivery system. The morphology of the microspheres and beads, the drug content and loading efficiency, the interaction between GA and PDLLA, and the drug release behaviors were studied. Scanning electron microscope (SEM) revealed that the PDLLA microspheres were homogeneously distributed in the beads. Differential scanning calorimetry (DSC) measurement suggested certain interaction between GA and PDLLA, and the crystal structure of GA was influenced by the polymer. The dual release in vitro showed a rapid BSA release but a sustained GA release in all the systems. Furthermore, the release rate of BSA was accelerated by increasing PDLLA/alginate ratio, while the release rate of GA was decreased, and the release of both hydrophobic and hydrophilic drugs could be adjusted by changing the ratio of PDLLA/alginate. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 20122
研究领域:
药物递送
·
生物材料
·
制药工程
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产品关联
DOI
高影响因子
BIOMATERIALS
·
2025
·
IF 12.9
Qingzhou Kong,Yinuo Zhao,Haifan Gong,Luoyao Kang,Jialu Fu,Lixiang Li,Boyao Wan,Peizhu Wang,Xiaojuan Li,Yue Wang,Jinghui Zhang,Yanbo Yu,Xiaoyun Yang,Xiuli Zuo,Haina Wang,Yanqing Li
Antimicrobial peptides (AMPs) offer a route to counter resistant pathogens but are often hampered by proteolysis, whereas D-peptides resist proteases yet remain underexplored due to data scarcity and design challenges. Here, we present PeptiD-Agent, a purely agent based framework that predicts D-peptide antimicrobial activity with extremely limited data, enabling rapid discovery of potent candidates. Using this approach, we identified DA2, a D-enantiometric AMP lead with broad-spectrum activity against drug-resistant bacteria and minimal hemolytic toxicity. DA2 showed high stability under physiological conditions, including resistance to enzymatic degradation and serum. Mechanistic studies indicate that DA2 exerts bactericidal effects by disrupting the integrity of the bacterial membrane in concert with multiple synergistic mechanisms. In murine models of skin wounds and intraperitoneal infection, DA2 conferred significant protection against drug-resistant pathogens and, when delivered via hydrogel, accelerated wound healing. These findings establish a computational route to potent, stable D-peptide antimicrobials and provide a general strategy for AMP design in data-scarce settings.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Catalysis Science & Technology
·
2024
·
IF 4.4
Qingxiu Wang,Weisong Liu,Lijing Chang,Kuncheng Zhang,Yanbing Shen,Lingling Zhang
L-guluronic acid, a patented drug with immunomodulatory and anti-inflammatory properties, is traditionally sourced from natural seaweed species or synthesized chemically. Both methods suffer from low selectivity and environmental concerns and there is a high demand to develop a green and efficient approach for L-guluronic acid production. Herein, we successfully developed a two-step enzymatic route to synthesize L-guluronic acid from D-glucose by two oxidases, glucose oxidase (GOx) and galactose oxidase (GOase). Due to the sluggish kinetics of GOase in catalyzing the oxidation of D-gluconic acid, rational design was implemented. Through molecular docking and iterative saturation mutagenesis, a variant, GOase T406R/K330R/Y329F, was obtained. The variant exhibited a remarkable 13-fold higher oxidation activity towards D-gluconic acid, reaching 7.8 U mg-1. The substitutions synergistically switch the D-gluconic acid molecule to a favorable conformation and facilitate the specific activation of the primary alcohol group at C6 position. In a cascade reaction, complete conversion of 40 mM D-glucose to L-guluronic acid was achieved in 2 h with 100% selectivity, presenting a promising and green bio-manufacturing strategy for the scalable production of L-guluronic acid.
研究领域:
生物催化
·
酶工程
·
绿色化学
·
药物
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jing Zhang,Ye Yang,Yi-Ping Liu,Guo-Cong Liu,Xi Zhu,Xin-Yu Jiang,Jin-Gang Yu
Electrochemical sensors have been considered as one of the most important methods to determine amino acid (AA) enantiomers. An interesting and highly-efficient electrochemical sensor, gold nanoparticles/D-sorbitol modified glass carbon electrode (Au NPs/D-sorbitol/GCE), was developed for the chiral recognition of Tyrosine (Tyr) enantiomers. Au NPs/D-sorbitol/GCE sensor exhibited excellent chiral recognition efficiency for Tyr enantiomers (I L /I D = 1.99, ∆Ep = 36 mV) by square wave voltammetry (SWV). The possible recognition mechanism was also proposed by cyclic voltammetry (CV) at different scanning rates, wettability measurements, and density-functional theory (DFT) calculations. The smaller steric hindrance and more suitable molecular distance are beneficial to the selective formation of hydrogen bonds between L-Tyr and the chiral interface of the sensor. The energy difference caused by the formation of hydrogen bonds between the chiral interface and Tyr enantiomers can be expressed as different peak currents to achieve chiral recognition. The redox peak current of Tyr enantiomers at Au NPs/D-sorbitol/GCE showed good linear relationship with their concentration, and the limits of detection (LOD) were calculated to be 4.12 μM and 8.07 μM for L-Tyr and D-Tyr, respectively. Importantly, Au NPs/D-sorbitol/GCE can also predict the mass ratios of L-Tyr in the enantiomeric mixtures. In addition, the sensor has shown good anti-interference ability, high stability, and excellent reproducibility.
研究领域:
分析化学
·
电化学
·
生物传感
·
手性分析
·
纳米材料
·
分子识别
·
氨基酸检测
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产品关联
DOI
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2024
·Vol. 22
·期 14
·2764-2773
·
IF 2.9
Yue Song,Wenjie Fan,Chen Yao,Heng Wang,Xiuxiang Lu,Yumin Wang,Pengxiang Liu,Yanjie Ma,Zhen Zhang,Jiang Wang,BeiBei Chu,Lijun Shi,Guoyu Yang,Mengdi Wang
Pattern recognition receptors (PRRs) play a critical role in the innate immune response, and toll-like receptor 7 (TLR7) is an important member of PRRs. Although several TLR7 agonists are available, most of them are being tested clinically, with only one available on the market. Thus, it is imperative to develop new TLR7 agonists. In this study, we designed and synthesized three kinds of quinazoline derivatives and five kinds of pyrrolo[3,2-d]pyrimidine derivatives targeting TLR7. The antiviral efficacy of these compounds was evaluated in vitro and in vivo. Our findings indicated that four kinds of compounds showed exceptional antiviral activity. Furthermore, molecular docking studies confirmed that compound 11 successfully positioned itself in the pocket of the TLR7 guanosine loading site with a binding energy of −4.45 kcal mol−1. These results suggested that these compounds might be potential antiviral agents.
研究领域:
免疫学
·
药物化学
·
病毒学
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DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Yutong Tong,Butian Chen,Chong Liu,Ruoyu Wang,Dewen Wang,Tianran Zhang,Xiaobai Ma,Limei Sun,Jinbo Yang,Wenyun Yang,Xiangfeng Liu
Halide solid electrolytes (SEs) show great potential in all-solid-state batteries (ASSBs) because of their high ionic conductivity and high oxidative stability. But the long-term cycling stability remains a major concern due to the cathode/halide interface side reactions, especially under high current density. Herein, a s-p-d orbital hybridization strategy is proposed via introducing Zr 4+ and F − into Li 3 InCl 6 (LIC-ZrF 4 ) to regulate the electron localization structure around the central In 3+ . The electron transfer between the cation and Cl is alleviated by the enlarged bandgap (4.485–4.894 eV) with ZrF 4 introduction, and more electrons aggregate around In 3+ after s-p-d hybridization among Zr, In, and Cl. This effectively prevents LIC-ZrF 4 from being attacked by O atoms in LiNi 0 . 8 Co 0 . 1 Mn 0 . 1 (NCM811) and significantly suppresses the interface side reactions. The in situ formed LiF-containing layer further enhances the interface stability. The assembled ASSB (NCM811/LIC-ZrF 4 /LPSC/Li-In) exhibits a state-of-the-art capacity retention (96.7%@500cycles@1C; 80.7%@1600 cycles@2C). Furthermore, the ionic conductivity of LIC-ZrF 4 is also enhanced from 0.6 to 1.5 mS cm −1 while the electronic conductivity is decreased from 3.66 × 10 −8 to 8.39 × 10 −9 S cm −1 . This work highlights the crucial role of s-p-d hybridization on the electron localization regulation and the interface stability, and presents some distinct insights into developing high-performance halides-based ASSBs.
研究领域:
材料科学
·
电化学
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Jiancheng Zhao,Zhaohui Guo,Christian Marcus Pedersen,Lingyu Jia,Shiyu Jia,Xianglin Hou,Yingxiong Wang,Yan Qiao
Organonitrogen chemicals are widely used and in great demand in the food, pharmaceutical and chemical industries. Hence, it is important to develop green and efficient synthesis paths to organonitrogen chemicals for their use as new resources. Herein, we propose the preparation of organonitrogen chemicals from chitin biomass monomer N -acetyl- d -glucosamine (GlcNAc) promoted by natural deep eutectic solvents (NADESs). The selective generation of 3-acetamido-5-(1′,2′-dihydroxyethyl) furan (Chromogen III) and 3-acetamido-5-acetylfuran (3A5AF) from GlcNAc was achieved by the design of NADESs components. GlcNAc reacts in binary NADES proline-glycerol (Pro-Gly) to generate 26 % of Chromogen III and 39 % of 3A5AF in ternary NADES proline-glycerol-lactic acid (Pro-Gly-LA). In addition, the intermolecular hydrogen bonds in NADESs were confirmed through 1 H diffusion-ordered spectroscopy (DOSY) NMR technology. And the results of 13 C NMR chemical shift titration indicated interaction between NADESs and the C3 and C6 position of GlcNAc. Based on the NMR experiments a mechanism is proposed.
研究领域:
生物化学
·
绿色化学
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产品关联
DOI
高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
·
2025
·
IF 4.2
Huilin Zhao,Shuzhen Liu,Jiangfan Shan,Juan Zhang,Qianwen Wu,Jianhui Zhang,Zekun Sun,Xinying Cao,Shu Chen,Mingzhu Feng,Yinuo Zhang,Xiang Cheng,Boqing Li,Xiaofei Ji
CagA, one of the key virulence factors of Helicobacter pylori , plays a significant role in H. pylori -associated gastric cancer by actively participating in neoplastic transformation. Among CagA variants, East Asian type CagA (CagA E ) bearing the EPIYA-D motif exhibits a higher risk than the Western-type (CagA W ) with EPIYA-C motifs. In this study, we investigated the interactions of CagA E and CagA W and host intracellular Grb2 and found a pronouncedly greater recruitment of Grb2 by CagA E compared to CagA W . Our findings revealed the phosphorylated tyrosine in the EPIYA motif is very important for the binding of CagA to Grb2. Phe at Y + 5 position in EPIYA-D, which interacts with Trp 121 in SH2 domain of Grb2, established the higher affinity than the interaction of EPIYA-C and Grb2-SH2. The substitute of Phe to Asp/Ala in CagA E EPIYA-D reduced the recruitment of Grb2 by CagA E , and neutralized the stronger induced malignant characteristics of the recipient cells. These findings provide additional insights into the distinct regulatory mechanisms employed by CagA E and CagA W , contributing to a better understanding of the higher oncogenic risk associated with H. pylori CagA E .
研究领域:
微生物学
·
分子生物学
·
癌症生物学
·
传染病学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）