AQUACULTURE INTERNATIONAL
·
2026
·Vol. 34
·期 7
·305
·
IF 3.5
Lu Liushen,Li Jinlong,Qin Ting,Chen Kai,Xie Jun,Ping Fang,Xi Bingwen
Aeromonas spp. are major pathogens severely affecting the Carassius auratus gibelio farming industry. The prolonged use of enrofloxacin (Enr) has led to reduced therapeutic efficacy, drug residues, and the emergence of antibiotic resistance. Simply increasing antibiotic dosage is unsustainable, and the core challenge lies in potentiating antibiotic efficacy without dose escalation. Here, we employed combination therapy using allicin E (ALE) as a potentiator of Enr and investigated its synergistic antibacterial activity, underlying mechanisms, and protective effects against A. hydrophila in C. auratus gibelio . In vitro, the combination of Enr and ALE exhibited broad-spectrum synergistic or additive activity against multiple aquatic pathogens, rapidly eradicating A. hydrophila without regrowth during the observation period. Mechanistically, the combination triggered a burst of bacterial reactive oxygen species (1.67-fold vs. control), leading to irreversible membrane damage and leakage of intracellular contents, and significantly inhibited biofilm formation and disrupted mature biofilms. In vivo, oral administration of the combination in C. auratus gibelio challenged with A. hydrophila significantly improved survival (66.6% vs. 26.6% in model), reduced bacterial loads in liver, spleen and kidney (by 70.9%, 81.3% and 72.9%, respectively, vs. model group), and mitigated oxidative damage by restored antioxidant enzymes (T-SOD, CAT) activity, decrease MDA, as well as attenuated inflammatory infiltration and hemorrhage in spleen and kidney tissues. This is the first report of ALE potentiating Enr against A. hydrophila via a synergistic ROS-mediated membrane damage, validated in C. auratus gibelio , with approximately 50% Enr reduction potential.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Jiahuan Ling,Rui Chen,Minghai Wang,Chun-Xiao Yan,Ruomu Xia,Lihui Zhang
Ergothioneine (EGT) is a powerful and natural antioxidant, which can protect cells in the human body from oxidative damage. Numerous studies have attempted to enhance the heterologous synthesis. However, research on EGT transport out of Escherichia coli cells and the wild-type enzyme from Trichoderma reesei is rare. Here, membrane permeability engineering and protein engineering are combined to improve EGT production in E. coli. After metabolic engineering to enhance precursor supply, we deleted the genes involved in lipopolysaccharide biosynthesis to increase the membrane permeability, which promoted EGT production. Further, a mutant of Tregt2E155C with improved catalytic capacity was created, and the expression level of enzymes involved in EGT synthesis was optimized. Finally, fermentation parameters were systematically tuned to maximize the EGT production. The engineered strain MT9-PET-T1/RSF-T2E155C produced 334.20 ± 6.33 mg/L EGT during 48 h of shake-flask fermentation, corresponding to an 8.4-fold increase compared with wild-type strain MT1. When scaled up to 5 L bioreactors, the strain achieved a final EGT titer of 4.06 g/L within 96 h (42.29 mg/L/h). Our work shows significant implications for EGT synthesis and is also a reference for manipulating E. coli to synthesize other biomolecules.
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MACROMOLECULAR BIOSCIENCE
·
2021
·Vol. 21
·期 4
·2000375
·
IF 4.979
Wenjuan Wei,Nouredine Behloul,Weiqi Wang,Sarra Baha,Zhenzhen Liu,Ruihua Shi,Jihong Meng
In a continuous effort to develop effective vaccines against hepatitis E (HE), oral vaccine nanoparticles using the truncated capsid protein p146 (aa460–605) are formulated and characterized. To improve the immunogenicity of p146, chitosan nanoparticles (CSNPs) are used as a mucosal delivery system. Next, the physical-chemical properties, cytotoxic effects in vitro, and immunogenicity in mice of the produced NPs are analyzed. The results show that the produced CS/p146 NPs are stable and well dispersive and display a near-spherical shape with a mean size of 200–300 nm. The findings also demonstrate high encapsulation efficiency (65–73.9%) and loading capacity (27.7–67.5%) of the formulated nanoparticles. Further, the CS/p146 NPs exhibit low cytotoxicity and an obvious sustained-release effect in vitro. Immunogenicity experiments in mice indicate that CS/p146 NPs can induce antigen-specific systemic and mucosal immune responses higher than the purified p146 do. Besides, the expression levels and mRNA transcription of Interleukin (IL)-4 in spleen cells of CS/p146 NPs-immunized mice are higher than those of p146, indicating that a Th2-mediated cellular immune response is activated by the CS/p146 NPs. Overall, the synthesized CS/p146 NPs display promising properties as a potential HE oral vaccine candidate.
研究领域:
病毒学
·
药学
·
免疫学
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Jianying Huang,Yuhong Liu,Lijun Yang,Fengyan Zhou
A sulfonated chitosan (SCS) was prepared via nucleophilic substitution and characterized by Fourier transform infrared spectroscopy, 1 H and 13 C NMR spectra, gel permeation chromatography, elemental analysis, and thermo gravimetric analysis. The inhibition activities of bacterial adhesion and biofilm formation against E. coli and S. aureus of SCS were assessed in comparison with those of unmodified chitosan hydrochloride (WCS) which was commercially available. The metabolic activity and secretion of exopolysaccharide in biofilms of E. coli and S. aureus were significantly decreased after the treatment of SCS. Scanning electron microscopy and confocal laser scanning microscopy also demonstrated that SCS and WCS at 1MIC concentrations could obviously inhibit the formation of biofilm. Upon the experimental data obtained, it can be concluded that the alkylsulfonation of chitosan could significantly improve efficacy in killing biofilm-embedded bacteria, and the inhibition activities against biofilm formation of E. coli and S. aureus . The exploitation of SCS in this study is helpful to extend the understanding to an alternative to antibiotics and chemical preservatives in food and medicine fields.
研究领域:
材料科学
·
微生物学
·
生物技术
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RSC Advances
·
2015
·Vol. 5
·期 37
·29185-29192
·
IF 3.84
He Li,Jun-Mian Tian,Hao-Yu Tang,Shi-Yin Pan,An-Ling Zhang,Jin-Ming Gao
Fifteen aromatic polyketide metabolites, including four new chromones, chaetosemins B–E (2–5), with compound 4 bearing a new skeleton, and two new natural products, chaetosemin A (1) and (+)-(S)-chaetoquadrin J (14), together with nine known ones (6–13, 15), were isolated from the organic extract of a solid fermented culture of an Ascomycete filamentous fungus, Chaetomium seminudum. The structures of 1–5 were determined by spectroscopic analysis, and the absolute configuration of chaetosemin A (1) was confirmed by X-ray crystallography, whereas the stereochemistry of 1–4 and 14 was assigned by comparison of their specific optical rotations with reported data for structurally related compounds. All compounds were isolated for the first time from Chaetomium seminudum. Compounds 1 and 2 consisted of L-cysteine and D-cysteine units, respectively. Most of them were evaluated for in vitro antifungal activities against some phytopathogenic fungi. Among them, chaetosemin B (2) was the most active against Magnaporthe oryzae and Gibberella saubinettii with MICs of 6.25 and 12.5 μM, respectively. In addition, chaetosemin C (3) showed antioxidant activity with 50.7% DPPH free radical scavenging activity at 50 μM, and chaetoquadrin J (14) displayed weak inhibition of soluble epoxide hydrolase (sEH).
研究领域:
天然产物化学
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真菌学
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药用微生物学
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生物有机化学
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抗真菌研究
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WATER RESEARCH
·
2022
·
IF 13.4
Yi Liu,Wenhua Dong,Shuyi Shen,Fanxu Meng,Jiajia Wang,Kun Yang,Daohui Lin
Cost-effective disinfection technology is urgently needed in poor rural areas. Erythrosine (ERY)-based solar disinfection (SODIS) provides a promising solution because of its effective inactivation of viruses and gram-positive bacteria at low cost. However, the poor gram-negative bacteria (G − , e.g., Escherichia coli ) inactivation of photosensitized ERY inhibits its application. Herein, for the first time, the protonation of ERY was found to greatly enhance its G − inactivation, and 99.99999% (7.0 log) of E. coli were completely inactivated within only 30 s using 2.5 mg/L ERY under 200 mW/cm 2 visible light irradiation. The inactivation rate constant ( k ) reached 17.5 min −1 at pH 4.0, which was 4730 times higher than that at pH 7.0. At a lower pH, more severe cell wall and genomic DNA damage was observed. A linear correlation between k and monoanionic ERY (HE − ) content was obtained, indicating that HE − rather than dianionic ERY (E 2− ) participated in the inactivation at pH 5.0–7.0, which was further explained by the higher production of reactive oxygen species and bacterial adsorption of HE − than E 2− . Both 1 O 2 and O 2 − • dominated bacterial inactivation, contributing 56.8% and 43.2%, respectively. O 2 − • but not 1 O 2 caused ERY photobleaching. OH • was not involved in either inactivation or photobleaching. Humic acid and salts (NaCl, Na 2 SO 4 , CaCl 2 , and MgCl 2 ) slightly inhibited inactivation, while NaHCO 3 accelerated inactivation. Complete inactivation (99.9999%) of E. coli was achieved within ∼30 min at pH 5.0 in ERY-based SODIS with good adaptation to various water matrices and weather (sunny or partly cloudy). This work will help to promote the application of ERY-based disinfection especially for SODIS in poor rural areas.
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JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Xiaoman Xie,Xirui Tan,Yiyan Yu,Yunchong Li,Pengbo Wang,Yuanhao Liang,Yunjun Yan
Conventional mining processes of rare earth elements (REEs) usually produce REEs-rich industrial waterwastes, which leads to a significant waste of REEs resources and causes serious environmental pollution. Biosorption using engineered microorganisms is an attractive technology for the recovery of REEs from aqueous solution. To regulate the REEs’ adsorption and recovery by sensing extraneous REEs, an engineered cascaded induction system , pmrCAB operon containing a lanthanide-binding tag (LBT) for sensing REEs, was incorporated into E. coli in conjunction with a silica-binding protein (Si-tag) and dLBT anchored onto the cell membrane. The sensing and adsorption capacities for Terbium (Tb), a typical study subject of REEs, were enhanced by screening an effective LBT and increasing the dLBT copy number. The adsorption capacity for Tb reached the highest reported value of 41.9 mg g −1 dry cell weight (DCW). After adhering the engineered cells onto the silica column surface through overexpressed Si-tag, a high recovering efficiency (> 90%) of Tb desorption could be obtained with 3 bed volumes of citrate solution. In addition, the engineered cells also possessed fairly good adsorption capacity of other tested REEs. Our findings showed that the recovery of REEs with high efficiency, selectivity and controllability from aqueous solution can be well achieved via specifically bio-engineered strains.
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Nano-Micro Letters
·
2021
·Vol. 13
·期 1
·1-14
·
IF 16.419
Lin Xiuzhu,Li Fan,Bing Yu,Fei Teng,Liu Sen,Zhao Hongran,Zhang Tong
A printable multifunctional electronic skin (e-skin) with an excellent self-healing ability was developed via a simple, clean and universally applicable method.
Water served as both the only solvent throughout the fabrication process and the trigger of the self-healing process. Each e-skin component was biocompatible.
Combined with conventional electronics, signals collected by the e-skin could be transmitted to smartphones via Bluetooth for post-processing.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Ting Zheng,Xiaoming Jiang,Naiwen Li,Xia Jiang,Chao Liu,Jing-Juan Xu,Peng Wu
Escherichia coli O157:H7 ( E. coli O157:H7 ) is typically associated with food- and water-borne outbreaks, thus making continuous monitoring essential. Photoelectrochemical (PEC) detection, which harvests the unique photo-to-electric property of photoactive species, has emerged as an exciting tool for bioanalysis. However, the current attempts are mostly carried out in lab, and less explored in field. Here, a simple PEC aptasensor for E. coli O157:H7 was designed and optimized (LOD: 200 cfu/mL). To promote its field application, a portable PEC analyzer was further constructed with a miniaturized LED (3 V, 3 W) for excitation and a USB-type electrochemical workstation for detection. Meanwhile, a re-chargeable power bank (5 V, 20,000 mAh, originally used for cell phone charging) was explored to power the LED through a self-designed electrical circuit. In this manner, the PEC analyzer could work continuously for more than 12 h. To facilitate transportation or the portable PEC analyzer, all components were mounted into a suitcase (size: 42 × 34 × 17 cm 3 ; wight: ∼3.5 kg). To validate the applicability of the portable PEC analyzer, on-site detection of E. coli O157:H7 in an in-land river was carried out (> 8 h), demonstrating the bright potential of the portable PEC analyzer for field environmental monitoring.
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Materials Science & Engineering C-Materials for Biological Applications
·
2021
·
IF 7.328
Chen Yu,Hengchang Zang,Cui Yang,Dong Liang,Shuang Quan,Danyang Li,Yanni Li,Qin Dong,Fengshan Wang,Lian Li
Osteoarthritis (OA) is a degenerative joint disease which is highly prevalent worldwide. However, no therapy for blocking OA pathogenesis is available currently. In this study, chondroitin sulfate (CS) E oligosaccharides were prepared and we identified disaccharide as the functional unit showing the strongest anti-complement activity and screened out complement C5 as its target in the complement system. We determined that CS-E disaccharide produced anti-inflammatory effects to treat OA by regulating the complement system: it inhibited the formation of complement-dependent complexes such as the membrane-attack complex (MAC) by targeting C5 and suppressed MAC-induced protein expression and the activation of downstream MAPK and NF-κB signaling pathways accordingly. By identifying CS-E disaccharide which could be regarded as a complement regulator or inhibitor exhibiting high anti-complement activity and revealing its OA-alleviating mechanism, this study not only provides a new strategy for OA treatment and drug development, but also potentially offers a promising C5 target therapy for other associated diseases.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Wenjing Huang,Zhujun Zeng,Yan Lang,Xia Xiang,Gaofu Qi,Gan Lu,Xiangliang Yang
In recent decades, epidemiological, clinical, and experimental studies have demonstrated that a diet with antioxidant or anti-inflammatory function plays a central role in the prevention of atherosclerosis (AS). The purpose of this study was to explore the effects of Cannabis seed oil (CO) administration on in vitro antioxidant capacity as well as blood lipid profiles, lipid peroxidation, inflammatory response, and endothelial cell integrity. Female ApoE–/– mice were fed a high-cholesterol diet and administrated with CO or phosphate-buffered saline (PBS) and seal oil by gavage for 8 weeks. The results show that CO administration reduced the levels of serum triglycerides and low-density lipoprotein cholesterol at week 6. Additionally, a decrease in serum tumor necrosis factor α and nitric oxide was also observed. Moreover, results from CD31 staining and scanning electron microscopy revealed that CO treatment alleviated the endothelial cell damage and lipid deposition induced by a high-cholesterol diet. The ratio of lesion area to the total aorta area was 19.57% for the CO group, which was lower than the PBS control group (24.67%). Collectively, CO exerted anti-atherosclerotic effects by modulating serum lipid profiles and inflammatory responses and improving endothelial cell integrity and arterial lipid deposition. The results provide a promising preventive strategy for the early progression of AS.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 101
·期 10
·4220-4228
·
IF 3.639
Shuang Gu,Zhenhe Wang,Wei Chen,Jun Wang
BACKGROUND Rice grains can be contaminated easily by certain fungi during storage and in the market chain, thus generating a risk for humans. Most classical methods for identifying and rectifying this problem are complex and time-consuming for manufacturers and consumers. However, E-nose technology provides analytical information in a non-destructive and environmentally friendly manner. Two-feature fusion data combined with chemometrics were employed for the determination of Aspergillus spp. contamination in milled rice. RESULTS Linear discriminant analysis (LDA) indicated that the efficiency of fusion signals (‘80 th s values’ and ‘area values’) outperformed that of independent E-nose signals. Linear discriminant analysis showed clear discrimination of fungal species in stored milled rice for four groups on day 2, and the discrimination accuracy reached 92.86% by using an extreme learning machine (ELM). Gas chromatography–mass spectrometry (GC–MS) analysis showed that the volatile compounds had close relationships with fungal species in rice. The quantification results of colony counts in milled rice showed that the monitoring models based on ELM and the genetic algorithm optimized support vector machine (GA-SVM) (R 2 = 0.924–0.983) achieved better performances than those based on partial least squares regression (PLSR) (R 2 = 0.877–0.913). The ability of the E-nose to monitor fungal infection at an early stage would help to prevent contaminated rice grains from entering the food chains. CONCLUSIONS The results indicated that an E-nose coupled with ELM or GA-SVM algorithm could be a useful tool for the rapid detection of fungal infection in milled rice, to prevent contaminated rice from entering the food chain. © 2021 Society of Chemical Industry
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