MOLECULAR NEUROBIOLOGY
·
2020
·Vol. 57
·期 12
·5324-5335
·
IF 4.5
Chen Guang,Gao Cheng,Yan Ya’nan,Wang Tao,Luo Chengliang,Zhang Mingyang,Chen Xiping,Tao Luyang
Intracerebral hemorrhage (ICH) is a form of stroke, characterized by high morbidity and mortality and currently lacks specific therapy. ICH leads to endoplasmic reticulum (ER) stress, which can induce neurological impairment through crosstalk with programmed cell death (PCD). Pyroptosis, a newly discovered form of PCD, has received attention because of its close relationship with some certain diseases, such as traumatic brain injury and ischemic and hemorrhagic stroke. However, the relationship between ER stress and pyroptosis in ICH remains unclear. In this study, we investigated the role of ER stress in evoking neuronal pyroptosis and related mechanisms in a mouse ICH model. We used tauroursodeoxycholic acid (TUDCA) to inhibit ER stress and observed that TUDCA reduces neuronal pyroptosis and has a neuroprotective role. We explored the potential mechanisms underlying the regulation of neuronal pyroptosis by ER stress through testing the expression of interleukin-13 (IL-13). We found that ER stress inhibition alleviates neuronal pyroptosis through decreasing the expression of IL-13 after ICH. In summary, this study revealed that IL-13 is involved in ER stress–induced neuronal pyroptosis after ICH, pointing to IL-13 as a novel therapeutic target for ICH treatment.
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Bioactive Materials
·
2020
·
IF 8.724
Yawei Ren,Yajing Han,Zhaoyang Li,Xiangmei Liu,Shengli Zhu,Yanqin Liang,Kelvin Wai Kwok Yeung,Shuilin Wu
Bacterial infection and related diseases are threatening the health of human beings. Photocatalytic disinfection as a simple and low-cost disinfection strategy is attracting more and more attention. In this work, TiO 2 nanoparticles (NPs) were modified by co-doping of Ce and Er using the sol–gel method, which endowed TiO 2 NPs with enhanced visible light photocatalytic performance but not pure ultraviolet photocatalytic properties compared the untreated TiO 2 . Our results disclosed that as the doping content of Er increased, the photocatalytic activity of modified TiO 2 NPs initially increased and subsequently decreased. The same trend occurred for Ce doping. When the doping dose of Er and Ce is 0.5 mol% and 0.2 mol%, the 0.5Ce0.2Ti–O calcined at 800 °C presented the best antibacterial properties, with the antibacterial efficiency of 91.23% and 92.8% for Staphylococcus aureus and Escherichia coli , respectively. The existence of Er ions is thought to successfully turn the near-infrared radiation into visible region, which is easier to be absorbed by TiO 2 NPs. Meanwhile, the addition of Ce ions can effectively extend spectral response range and inhibit the recombination of electrons and holes, enhancing the photocatalytic disinfection activity of co-doped TiO 2 .
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 14
·3823-3831
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IF 3.125
Guo Xiaofei,Wu Shijia,Duan Nuo,Wang Zhouping
A novel aptasensor labeled with Mn 2+ -doped NaYF 4 :Yb/Er upconversion nanoparticles (NaYF 4 :Yb,Er/Mn UCNPs) was employed in electrogenerated chemiluminescence (ECL) for the sensitive detection of bisphenol A (BPA). The ECL aptasensor was assembled by immobilizing the thiolated aptamers of BPA covalently on a gold nanoparticle (AuNPs)-modified electrode and pairing with complementary DNA labeled with NaYF 4 :Yb,Er/Mn UCNPs. The ECL aptasensor can not only rapidly and accurately detect BPA concentrations from 0.05 to 100 ng/mL with a detection limit of 0.037 ng/mL but also provides a new platform for ECL applications based on the use of upconversion nanoparticles as a promising alternative material.
The NaYF4:Yb,Er/Mn UCNPs combining with the BPA aptamer serving as recognition elements create a ECL platform for the sensitive detection of bisphenol A. The change in ECL signals induced by aptamer-target interactions was measured and a significant decrease in intensity was found on interaction with BPA in the concentration range of 0.05 to 100 ng/mL.
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JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2016
·Vol. 63
·期 12
·977-984
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IF 0.879
Rongjin Sun,Ting Yin,Peng Huang,Guo Gao,Joseph George Shapter,Yulan Shen,Jingjing Zhang,Daxiang Cui
In this paper, we report the development of a facile one-pot hydrothermal method for the controlled synthesis of monodispersed BaGdF 5 :Yb/Er nanoparticles for computed tomography (CT) and magnetic resonance (MR) imaging. The as-prepared nanoparticles have uniform size with a diameter of 20–25 nm. MTT tests show that the cell viability surpasses 90% even with a concentration of nanoparticles of 500 µg/mL, suggesting that the as-prepared BaGdF 5 :Yb/Er nanoparticles possess low toxicity. For both BaGdF 5 :Yb/Er nanoparticles and iopromide, the Hounsfield unit (HU) values increase with the their concentration. The slope of BaGdF 5 :Yb/Er nanoparticles is about 26.47, which is much higher than that of iopromide (16.98), indicating a better CT imaging effect. Interestingly, we find that the synthesized BaGdF 5 :Yb/Er nanoparticles exhibit paramagnetism. Our in vitro and in vivo imaging experiments demonstrate that the synthesized monodispersed BaGdF 5 :Yb/Er nanoparticles can serve as effective contrast agents for CT and MR imaging.
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Nature Communications
·
2025
·
IF 15.7
Hu Xuewen,Zhou Tiantian,Cui Tiansi,Du Yuanjiao,Song Chunyu,Chang Weiping,Xiong Juan,Qin Jichao,Deng Lin,Ji Wei-Ke
Sigma Non-Opioid Intracellular Receptor 1 (SigmaR1) is a member of the sigma family of receptors that interacts with a variety of psychotomimetic drugs and is involved in a wide range of cellular and physiological functions. Despite its increasing importance in human physiology and disease, the subcellular localization of SigmaR1 and its molecular function remain poorly defined. Using endogenous tagging and cell fractionation, we show that SigmaR1 is a type II integral ER membrane protein that is specifically enriched at ER sheets. A short region at the N-terminus of SigmaR1 promotes its ER-sheet localization. Importantly, our biochemical studies demonstrate that SigmaR1 directly interacts with components of the translocon complex including TRAPα and Nicalin. In addition, we found that a β-barrel at the C-terminal of SigmaR1 binds phosphatidylcholine (PC), and the binding of PC strengthens the association of SigmaR1 with the translocon complex. SigmaR1 knockout systematically impaired cellular protein and lipid homeostasis, resulting in accumulation of lipid droplets in hepatocytes. Collectively, we propose that SigmaR1 is an auxiliary translocon factor that binds lipids to regulate protein and lipid droplet homeostasis, which may underlie the broad and vital roles of SigmaR1 in physiology and disease.
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Nanomaterials
·
2021
·Vol. 11
·期 7
·1847
·
IF 5.076
Yan Ma,Yujie Miao,Guomei Mu,Dunmin Lin,Chenggang Xu,Wen Zeng,Fengyu Xie
Great expectations have been held for the electrochemical splitting of water for producing hydrogen as a significant carbon-neutral technology aimed at solving the global energy crisis and greenhouse gas issues. However, the oxygen evolution reaction (OER) process must be energetically catalyzed over a long period at high output, leading to challenges for efficient and stable processing of electrodes for practical purposes. Here, we first prepared Fe-MOF nanosheet arrays on nickel foam via rare-earth erbium doping (Er0.4Fe-MOF/NF) and applied them as OER electrocatalysts. The Er0.4Fe-MOF/NF exhibited wonderful OER performance and could yield a 100 mA cm−2current density at an overpotential of 248 mV with outstanding long-term electrochemical durability for at least 100 h. At large current densities of 500 and 1000 mA cm−2, overpotentials of only 297 mV and 326 mV were achieved, respectively, revealing its potential in industrial applications. The enhancement was attributed to the synergistic effects of the Fe and Er sites, with Er playing a supporting role in the engineering of the electronic states of the Fe sites to endow them with enhanced OER activity. Such a strategy of engineering the OER activity of Fe-MOF via rare-earth ion doping paves a new avenue to design other MOF catalysts for industrial OER applications.Keywords:rare-earth doping;MOFs;large current;electronic structure
研究领域:
材料科学
·
电化学
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Science of Advanced Materials
·
2017
·Vol. 9
·期 12
·2223-2233
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IF 1.671
Tan Haihu,Xie Shaowen,Xu Jianxiong,Li Na,Zhang Changfang,Xu Lijian,Zheng Jie
Rational design and fabrication of multimodal imaging nanoprobes are of great significance for many imaging applications. Here, we report the systhesis of water-soluble hexagonal NaYF4:Yb, Er up-conversion phosphors (UCPs) with branched structure by hexadecylpyridinium chloride
(CPC) and tri-sodium citrate (Na3Cit) binary cationic/anionic capping agents assisted hydrothermal reaction. The crystal phase, morphology, and size of NaYF4:Yb, Er UCP were controlled by the concentration of Na3Cit and CPC, molar ratio of NaF:RE3+,
hydrothermal temperature, hydrothermal time, as well as analyzed by transmission electron microscopy (TEM), high-resolution TEM (HRTEM), X-ray diffraction (XRD). By tuning and optimizing hydrothermal conditions, NaYF4 UCPs experienced the phase transition from pure cubic phase to
mixed cubic/hexagonal phases to pure hexagonal phase, as well as morphology changes from spherical nanoparticles to short pillar structures, and ultimately to branched structures. Due to the special geometrical shape, the branched NaYF4:Yb, Er UCPs could be blended with host materials
without further surface modification. As a proof-of-concept, branched NaYF4:Yb, Er phosphors were then incorporated into polyvinyl alcohol (PVA) matrix to develop a novel luminescence composite film. The resulting PVA/NaYF4:Yb, Er composite film possessed optical transparence
at daylight and emitted green fluorescence at the irradiation of a 980 nm laser. NaYF4:Yb, Er phosphors and hybrid PVA/NaYF4:Yb, Er demonstrate high potential of fluorescent materials used for anti-counterfeiting and other optical applications.
研究领域:
材料科学
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纳米技术
·
光学材料
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发光学
·
防伪技术
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Journal of Materials Chemistry C
·
2023
·Vol. 11
·期 31
·10483-10491
·
IF 6.4
Lei Wang,Xiaohong Ji,Qinyuan Zhang
Enhancing the near-infrared (NIR) photoresponse of molybdenum disulfide (MoS2) is essential for its photoelectric applications including photo communication. Herein, continuous Er-doped MoS2 (MoS2:Er) films were synthesized via a multi-step process. The enhanced NIR response of MoS2:Er at 808 nm and 980 nm is attributed to the characteristic absorption of Er3+ at both wavelengths and the increased traps introduced by Er-doping. The developed MoS2:Er/SiO2/p-Si heterojunction device possesses a rapid response time of ∼1.4 μs, a high detectivity of ∼3.67 × 1010 Jones, and a wide 3 dB bandwidth of 7.9 kHz at −0.5 V under 980 nm laser irradiation. The dark current of the heterojunction photodetector can be suppressed under 980 nm laser illumination for a period longer than 8 ms because of an extra light-generated field in the dark induced by the residual charges in the traps. Meanwhile, a NIR light communication system is demonstrated by employing the heterojunction detectors as the receiver. This work provides an alternative method to synthesize homogeneous metal-doped MoS2 films and an effective strategy to modulate the photoelectric performance of MoS2-based devices.
研究领域:
材料科学
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光电子学
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纳米技术
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光电探测器
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半导体物理
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CELL DEATH AND DIFFERENTIATION
·
2026
·
IF 13.6
Zhou Chen,Bai Dongsheng,Zhao Jiawei,Yin HongJie,Du Jiaying,Gu Chunyang,Wang Yuxiang,Lin Binyan,Lu Na,Zhao Yue
The cGAS-STING signaling serves as a central hub for innate immunity by orchestrating type I interferon production and inflammatory cascades in response to cytosolic DNA. A critical rate-limiting step in this pathway involves STING exit from the endoplasmic reticulum (ER), a process initiated by COPII-mediated trafficking that requires ER membrane curvature formation. However, the molecular mechanism that drives this process is poorly understood. In this study, we identify VMP1, an ER-resident transmembrane protein, as a negative regulator that constrains STING ER exit and downstream signaling activation. VMP1 controlled ER PtdIns(3)P accumulation, which was essential for ER membrane curvature formation. Depletion of Vmp1 triggered CDIPT-dependent PtdIns(3)P synthesis through a non-canonical pathway independent of VPS34 stability and MTMR3 expression. Furthermore, VMP1-mediated macroautophagy degraded Rab10 GTPase, thereby suppressing CDIPT recruitment to the ER membrane. Myeloid-specific Vmp1 knockout mice exhibited augmented antiviral defense against DNA virus infection but displayed unaltered susceptibility to RNA virus infection. These results suggest that VMP1 controls STING trafficking by modulating the Rab10-CDIPT axis to restrict ER PtdIns(3)P accumulation and limit ER membrane curvature formation required for COPII vesicle biogenesis.
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Science Advances
·
2026
·Vol. 12
·期 21
·
IF 13.9
Jiaojiao Wang,Jian Jiang,Jie Ren,Yunxi Zhao,Yu Zhang,Deyu Feng,Yanjie Li,Qian Zhang,Yue Niu,Xin Zhang,Kang Xu,Jiaqi Sun,Pengwei Wang,Huanquan Zheng,Diane C. Bassham,Chengyuan Wang,Junjie Hu,Yan Bao
The endoplasmic reticulum (ER) is essential for cellular homeostasis, yet plant-specific mechanisms regulating its structure remain poorly understood. Here, we uncover a functional partnership between the Arabidopsis dynamin-like GTPase RHD3 and the plant-specific DUF641 protein COST1 in maintaining ER morphology and plant growth. COST1 was previously isolated as a negative regulator of autophagy in the drought response. In this study, by using IP-MS, we identified COST1 as an ER-associated protein that interacts directly with RHD3, a key regulator of ER membrane fusion. COST1, despite lacking transmembrane domains, tightly associates with the ER membrane via RHD3, which recruits COST1 to ER three-way junctions. In addition, recruiting of COST1 to the ER can improve the dimerization of RHD3 and its protein stability. Biochemical assays revealed that COST1 promotes the GTP hydrolysis activity of RHD3 by ~20% and significantly stimulates its membrane fusion capacity. Structural modeling predicts a heterotetrameric complex entangled with each protein’s multiple helix bundles, where COST1’s carboxyl-terminal domain aligns with RHD3’s GTPase region, suggesting a mechanism for GTP hydrolysis regulation. Genetic analyses demonstrated that cost1 rhd3 double mutants exhibit exacerbated ER fragmentation and stunted growth compared with single mutants, underscoring their synergistic roles. Transcriptomic profiling linked these phenotypes to disordered stress responding and growth pathways. Our findings reveal a plant-specific regulatory module in which COST1 partners with RHD3 to govern ER architecture, bridging membrane dynamics with growth and stress adaptation, providing insights into ER maintenance mechanisms with plant innovations and the potential applications in improving crop resilience.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Xinyu Zhang,Haojie Liu,Yumeng Shi,Zhenhao Liu,Yan Wang,Simai Chen,Xingzhi Yan,Rongyao Xu,Junqing Ma,Qiang Chen,Shuyu Guo
Aims Temporomandibular joint osteoarthritis (TMJ OA) is a degenerative disease linked to disrupted chondrocyte activity and cartilage homeostasis. This study aimed to clarify the protective role of optineurin (OPTN) in preventing chondrocyte apoptosis during TMJ OA progression. Materials and Methods Using bioinformatics analysis, Optn was identified as a key gene in chondrocyte regulation. We employed a unilateral anterior crossbite (UAC) model in vivo and Tunicamycin (TM) in vitro to investigate OPTN's role in TMJ OA. Makin scores and histological staining assessed cartilage degeneration, while flow cytometry measured chondrocyte apoptosis, mitochondrial, and endoplasmic reticulum (ER) calcium levels. Key Findings We demonstrated that OPTN deficiency accelerates arthritis progression by intensifying endoplasmic reticulum stress (ERS) and chondrocyte apoptosis both in vivo and in vitro. Mechanistically, in vitro data showed that OPTN interacted with glycogen synthase kinase 3 beta (GSK3β), modulating the inositol 1,4,5-triphosphate receptor (IP3R)/glucose-regulated protein 75 (GRP75)/voltage-dependent anion channel 1 (VDAC1) complex. This interaction affected ER-mitochondrial calcium transfer, elevating mitochondrial calcium, and promoting apoptosis. Inhibiting this calcium channel with SB216763 and 2-APB effectively reduced mitochondrial Ca 2+ overload and apoptosis. Significance These findings reveal the crucial role of OPTN deficiency in TMJ OA pathogenesis and propose that targeting the IP3R-GRP75-VDAC1 complex to alleviate mitochondrial calcium could offer new therapeutic strategies for TMJ OA.
研究领域:
医学
·
分子生物学
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MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Huanhuan Li,Shujat Ali,Wenya Wei,Yi Xu,Haowei Lu,Md Mehedi Hassan,Xiangyang Wu,Min Zuo,Qin Ouyang,Quansheng Chen
In this study, a novel fluorescence approach for the rapid and selective detection of organophosphorus pesticides is presented. The strategy is based on inhibition of acetylcholinesterase (AChE) activity and NaY/GdF 4 :Yb, Er up-conversion nanoparticles (UCNPs) fabricated with manganese dioxide (MnO 2 ) nanosheets. Water-soluble UCNPs were first synthesized, and MnO 2 nanosheets were fabricated on their surface to get an efficient quencher for up-conversion fluorescence. Subsequently, AChE was used to restore the fluorescence by dissociation of MnO 2 nanosheets from the surface of UCNPs. When diazinon was added into the system, AChE activity was irreversibly impeded and resulted in the quenching of fluorescence. The inhibitory effect was linearly dependent on the concentration of the pesticide. Under the optimized conditions, the proposed sensor showed excellent performance for the detection of diazinon with a wide detection range of 0.1 to 30 ng/mL and a low detection limit (LOD) of 0.1 ng/mL. Besides, the system showed a good selectivity and was applied for diazinon detection in adulterated tea samples with a recovery rate of 98% to 111%. The unique advantages of the strategy such as simple, cost-effective, highly selective and sensitive make it an attractive approach for the detection of OPs in tea commodities.
研究领域:
分析化学
·
食品安全
·
纳米技术
·
生物传感
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