ULTRASONICS SONOCHEMISTRY
·
2020
·
IF 6.513
Mingming Guo,Lianjiao Zhang,Qiao He,Saifanassour Ali Arabi,Huanhuan Zhao,Weijun Chen,Xingqian Ye,Donghong Liu
Essential oil nanoemulsions have been proven to have stronger antimicrobial effects compared to the essential oil alone or coarse emulsion. Sonoporation could be the promising candidate to trigger a synergistic effect with thyme essential oil nanoemulsion (TEON) and produce a more effective antibacterial efficacy. Therefore, in this study, the bactericidal effects of ultrasound (US) in combination with TEON treatments against Escherichia coli ( E. coli ) O157:H7 were investigated. The remarkable synergistic effects of US (20 kHz, 255 W/cm 2 , 9 min) and TEON (0.375 mg/mL) treatments at 22 °C reduced E. coli O157:H7 populations by 7.42 ± 0.27 log CFU/mL. The morphological changes of cells exposed to different treatments were observed by scanning electron microscopy and transmission electron microscopy. The results showed that the synergistic effects of the ultrasound and TEON treatments altered the morphology and interior microstructure of organism cells. Laser scanning confocal microscopy (LSCM) images revealed that the combination treatments of ultrasound and TEON altered the permeability of cell membranes, and this affected the integrity of E. coli O157:H7 cells. This was further indicated by the high amounts of nucleic acids and proteins released from these cells following treatment. The results from this study illustrated the mechanisms of the synergistic effects of sonoporation and TEON treatments and provided valuable information for their potential in food pasteurization.
✓ 已验证
DOI
PubMed
高影响因子
PROTEIN EXPRESSION AND PURIFICATION
·
2015
·
IF 1.695
Fanqiang Meng,Haizhen Zhao,Chong Zhang,Fengxia Lu,Xiaomei Bie,Zhaoxin Lu
A potential bacteriocin gene was isolated from 18575 ORFs by bioinformatics methods. It was named pln1 , and cloned into pET32a. Then, it was expressed as a thioredoxin-Pln1 fusion protein in Escherichia coli BL21 (DE3). The fusion protein was purified by Ni-NTA, and thioredoxin was removed by enterokinase. Finally, Pln1 was purified using a cation affinity column. The yields of fused and cleaved Pln1 peptides were 100–110 mg/l and 9–11 mg/l, respectively. Pln1 was stable in an acidic environment and at temperatures below 60 °C, but was easily degraded under alkaline conditions and by protease treatment. The cleaved and purified Pln1 showed strong antimicrobial activity against gram-positive bacteria such as Micrococcus luteus CMCC 63202, Staphylococcus epidermidis , Lactococcus lactis NZ3900, Lactobacillus paracasei CICC 20241, and Listeria innocua CICC 10417. In particular, Pln1 had a better activity against methicillin-resistant S. epidermidis (MRSE) than nisin, thereby offering an attractive approach to counter bacterial antibiotic resistance .
✓ 已验证
DOI
PubMed
高影响因子
BMC MICROBIOLOGY
·
2025
·Vol. 25
·期 1
·805
·
IF 4.2
Zhou Qianyu,Xue Jinjing,Li Jun,Liu Zhiying,Zou Yinuo,Dai Rui,Gu Kuan,Zhou Yonglin
The advent and global spread of plasmid-carried resistance genes, including the tigecycline resistance gene tet (X3/X4) and the colistin resistance gene mcr-1 , present a substantial threat to public health that requires immediate resolution. This study successfully screened and identified commercial anti-parasitic drugs that efficiently inhibit Tet(X4) enzyme activity (IC 50 = 79.42 µg/mL), revealing that mefloquine exhibited a synergistic antibacterial effect with tetracyclines against tet (X3/X4)-positive bacteria (FIC < 0.5) and an additive effect with tetracyclines against tet (X3/X4)-negative bacteria (0.5 < FIC < 1, excluding minocycline). Molecular dynamics simulations and checkerboard minimum inhibitory concentration (MIC) tests on Tet(X4) mutants in amino acid residues demonstrated that mefloquine’s interaction with the active site of Tet(X4) reduces its activity. The energy contribution of the binding site residues involves PHE221, PHE316, GLY233, PRO315, PHE232, MET212, ALA222 and HIE231, which establish robust interactions with mefloquine (Δ Etotal of ≤ − 0.5 kcal/mol). Moreover, findings from metabonomics, bacterial membrane analysis, ROS detection, NAD + /NADH ratio determination, and intracellular ATP determination indicated that mefloquine may significantly influence bacterial energy metabolism ( P < 0.05). Furthermore, in the mouse infection model of E. coli 47EC, the combination of methacycline and mefloquine, demonstrated significant therapeutic advantages, as evidenced by an increased survival rate (from 20.00% to 80.00%), decreased sepsis score, and decreased bacterial load. Our work is the first to reveal that the commercial medicine mefloquine can prolong the efficacy of tetracycline and treat infections caused by drug-resistant Enterobacteriaceae in clinical environments. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Communications Biology
·
2020
·Vol. 3
·期 1
·1-10
·
IF 4.165
Liu Yuan,Jia Yuqian,Yang Kangni,Li Ruichao,Xiao Xia,Wang Zhiqiang
Recent emergence of high-level tigecycline resistance mediated by Tet(X3/X4) in Enterobacteriaceae undoubtably constitutes a serious threat for public health worldwide. Antibiotic adjuvant strategy makes antibiotic more effective against these resistant pathogens through interfering intrinsic resistance mechanisms or enhancing antibiotic actions. Herein, we screened a collection of drugs to identify compounds that are able to restore tigecycline activity against resistant pathogens. Encouragingly, we discovered that anti-HIV agent azidothymidine dramatically potentiates tigecycline activity against clinically resistant bacteria. Meanwhile, addition of azidothymidine prevents the evolution of tigecycline resistance in E. coli and the naturally occurring horizontal transfer of tet (X4). Evidence demonstrated that azidothymidine specifically inhibits DNA synthesis and suppresses resistance enzyme activity. Moreover, in in vivo infection models by Tet(X4)-expression E. coli , the combination of azidothymidine and tigecycline achieved remarkable treatment benefits including increased survival and decreased bacterial burden. These findings provide an effective regimen to treat infections caused by tigecycline-resistant Escherichia coli .
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xinghui Yu,Wenyang Shi,Junyan Hou,Kairui Zhai,Yang Xu,Jie Zhao,Xiaodong Lin
Escherichia coli ( E. coli ) is a leading cause of hospital-acquired infections, especially in abdominal and urinary tract sites, posing serious risks for patients’ post-kidney transplantation. Rapid and precise detection of E. coli is critical to prevent complications such as bloodstream infections, urinary tract infections, and infectious shock. Here, we present a prolong glow-type chemiluminescence (CL)-based bacterial metabolism platform that achieves both sensitive detection and effective photothermal inactivation of E. coli based on multifunctional nanomaterials. This platform leverages the metabolic activity of E. coli to trigger a CL signal without the need for external excitation sources, facilitating low-cost and highly sensitive detection with a limit of detection as low as 1 CFU/mL. Fe 3 O 4 NPs were functionalized with E. coil -specific antibodies, allowing for precise pathogen capture and magnetic separation, ensuring robust sample preparation. After detection, near-infrared (NIR) laser irradiation enabled the Cu 2-X Se NPs to generate localized heat, effectively inactivating E. coli cells with an antibacterial efficiency of up to 99.18%. This multifunctional platform integrates rapid detection and sterilization, demonstrating significant potential for clinical applications in infection management, especially in post-transplant care.
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Hongyang Chen,Zimao Ye,Yanchi Liu,Zhiqin Zhou
An integrated physiological and non-targeted metabolomics approach was used to construct a eugenol-based polymethoxyflavones nanoemulsion delivery system and examine its increased antibacterial activity on E. coli . Compared with pure eugenol nanoemulsion (EG, MIC: 640 μg/mL), the polymethoxyflavones-eugenol nanoemulsion (EGP) exhibited significantly enhanced antimicrobial activity (MIC: 320 μg/mL). EGP treatment disrupted the integrity of E. coli cell membranes and walls, increased cell permeability. Non-targeted metabolomics analysis (compared with CK) revealed 685 upregulated and 642 downregulated differentially accumulating metabolites, lysine and glutamine are reduced in E. coli , and glutaric acid, aminoadipic acid, and D-lactic acid are increased. Pathway enrichment analysis showed that amino acid metabolism and energy metabolism were significantly disturbed by TCA cycle disruption. EGP exhibits superior antimicrobial efficacy against E. coli through a multi-targeted mechanism involving cell membrane disruption and metabolic pathway interference. This study provides valuable insights into the development of natural antimicrobial nanoemulsion as potential alternatives to synthetic preservatives for food safety applications.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xi Li,Liusheng Lei,Jing Yu,Xiaowei Wu,Wenjin Hu,Huai Lin
Microplastics (MPs) are widely used and persist in the aquatic environment, leading to inevitable exposure for organisms, concerns have arisen regarding the potential health risks associated with MPs. However, current research has largely overlooked the microbiological effects of chronic exposure to MPs on bacteria, which have significantly health implications. Herein, we exposed susceptible Escherichia coli to an environmentally relevant concentration (1 mg/L) of polystyrene MPs over ~1800 generations (36 days). In parallel, the control group was serially passaged in MPs-free LB medium for 36 days. Our results reveal that the evolved E. coli cells ( E MP ) exhibited significantly increased resistance to oxidative stress (465-fold) and to multiple antibiotics including cefotaxime, ceftriaxone, and kanamycin, as evidenced by inhibition zone decreasing by 0.81–0.96-fold. Additionally, we observed physiological adaptations including flattened morphology, fitness cost, enhanced biofilm formation capacity, and increased cell motility, which decreased the cells' sensitivity to multiple stressors. Notably, these adaptive traits were associated with increased pathogenicity, as demonstrated by higher adhesion to macrophages and intestinal epithelial cells in vitro and more severe bacterial infections in Galler mellonella in vivo . Mechanistically, the observed adaptations were primarily attributed to the regulation of genes involved in the electron transport chain, energy metabolism and two-component systems, which are known to play crucial roles in bacterial stress responses. This study underscores the urgent need to consider and regulate the health risks posed by long-term exposure to persistent and harmful MPs.
✓ 已验证
产品关联
DOI
高影响因子
BIOTECHNOLOGY LETTERS
·
2026
·Vol. 48
·期 2
·44
·
IF 2.5
Ma Li,Wang Yuyu,Liu Ruiqi,Hu Jingjia,Zheng Xiaobing,Yue Xiaoyang,Liu Guanhua,He Ying,Zhou Liya
Developing stable and easy-to-operate biocatalysts is crucial for their use as industrial catalysts. Here immobilized whole-cell catalysts were used for β-alanine production by immobilizing recombinant Escherichia coli cells (containing hydroaminase) with diatomite. E. coli BL21 (DE3)/pET-30a ( +)-HAMase was genetically engineered for the efficient synthesis of β-alanine from acrylic acid and aqueous ammonia. Using glutaraldehyde as a cross-linking agent, polyethyleneimine (PEI) as a flocculant, and diatomite as the immobilization carrier, optimal immobilization was achieved with 8% (w/v) PEI solution, 5% (w/v) glutaraldehyde, and 100 mg wet cell/mL cell suspension, along with a PEI flocculation time of 2.5 h and glutaraldehyde cross-linking time of 1.5 h. The enzyme activity recovery rate reached 70.72%. Remarkably, the immobilized whole-cell catalysts exhibited excellent stability, retaining over 90% of initial enzyme activity after 12 h incubation at 45 °C and maintaining over 72% enzyme activity after storage for 60 days at 4 °C. Additionally, the immobilized cells demonstrated enhanced reusability, maintaining consistent β-alanine yield even after ten consecutive reaction batches with an average yield of approximately 80%.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Synthetic and Systems Biotechnology
·
2026
·
IF 4.8
Bo Zhang,Xintian Liu,Tianjun Zhao,Jinkang Hao,Zejian Wang,Menglei Xia,Ning Chen,Xiaonan Wang,Xiaoguang Fan
l -Histidine is an essential amino acid with important applications in pharmaceuticals and nutrition, highlighting the demand for efficient microbial production platforms. This study developed a high-performance Escherichia coli cell factory through systematic metabolic engineering. First, we mined a feedback-resistant hisG∗ smar and the entire mutant his operon from a previously obtained high l -histidine-producing mutant of Serratia marcescens , offering novel enzymatic parts beyond conventional sources. Combined with precursor supply enhancement and redox balancing, the engineered strain yielded 4.46 g/L l -histidine. Second, a machine learning-based platform (TransDW) was utilized to predict and validate a novel efflux transporter, Cgl1374, increasing titer to 4.82 g/L. Third, we implemented a growth phase-dependent system to dynamically regulate pgi expression, redirecting carbon flux and achieving 5.49 g/L in shake flasks. Finally, applying a novel carbon evolution rate (CER)-based control strategy in fed-batch fermentation, the optimized strain achieved 49.8 g/L of l -histidine in a 5-L bioreactor, with a yield of 0.265 g/g glucose, which is the highest yield reported for engineered E. coli . This work establishes a synergistic framework combining non-model gene discovery, computational transport engineering, and real-time physiological feedback control, providing a versatile blueprint for next-generation microbial cell factories.
✓ 已验证
DOI
PubMed
高影响因子
Nature Communications
·
2026
·
IF 18.1
Zhiyun Wang,Ziwei Chen,Sheng Wang,Luna Cui,Minghao Tang,Xianhua Liu,Tao Wang,Xueping Li
Microplastics (MPs), ubiquitous environmental pollutants, can enter the human body and exacerbate health risks. Polyvinyl chloride MPs (PVC-MPs) are emerging contaminants of particular concern due to direct clinical exposure pathway. However, the role of PVC-MPs in pathogenic bacterial infections remains poorly understood. Here, we investigate the role of PVC-MPs for the pathogenicity of uropathogenic Escherichia coli (UPEC), the primary causative agent of urinary tract infections (UTIs). Results demonstrate that PVC-MPs significantly enhance both acute and chronic UTIs. Mechanically, PVC-MPs act as carriers, enriching UPEC on their surface and enhancing UPEC invasion of bladder epithelial cells (BECs) via receptor-independent pathway, thereby promoting UPEC colonization in mouse bladders and kidneys. After entering BECs, intracellular PVC-MPs induce mitochondrial dysfunction, causing mitochondrial reactive oxygen species accumulation and mitochondrial DNA leakage, which activate Caspase-1-dependent pyroptosis, promoting urothelial cell shedding and UPEC chronic infection. These findings establish PVC-MPs as mediators of UTI pathogenesis, providing critical mechanistic insights into how environmental pollutant exposure may enhance susceptibility to infectious diseases. Polyvinyl chloride microplastics have been detected within clinical settings and may influence bacterial infections. Here, the authors demonstrate that these microplastics facilitate acute infection by uropathogenic E. coli, helping the bacteria evade immune cells and increase shedding.
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2025
·
IF 11.3
Xi Li,Zhigang Yu,Jiaqing Ye,Xiaolong Wang,Huai Lin,Liusheng Lei,Wenjin Hu,Yi Luo
Numerous disinfectants have been discharged into the environment through anthropogenic activities, leading to ubiquitous coexistence of multiple disinfectants in the real environment. Previous studies reported that individual disinfectants induce the emergence of antimicrobial resistance (AMR); however, the impacts of the coexposure to multiple disinfectants on the development of AMR and its toxicity associated with bacterial pathogenicity are still unclear. Here, we conducted a 30-day stepwise exposure experiment to investigate the impacts of the commonly used disinfectants polyhexamethylene guanidine (PHMG), benzalkonium chloride (BAC), and their combination on AMR and pathogenicity of Wild-Type Escherichia coli. Results reveal that the coexistence of dual disinfectants dramatically led to higher levels of AMR (2.0- to 14.8-fold) and pathogenicity (8.7-fold) in the evolved strains. Dual disinfectant-induced bacterial evolution is primarily driven by the alteration in the expression of key functional genes, such as those involved in biofilm formation and cell motility. In contrast, genetic mutations may not account for the increased AMR and pathogenicity in combination of dual disinfectant treatment, as similar gene mutations are responsible for both dual and single disinfectant stress. The evolved strains induced by disinfectant combination, characterized with transcriptional regulation, exhibited a synergistic or additive response on AMR and pathogenicity, which was further confirmed by a toxicology prediction model. This study demonstrated that dual disinfectants synergistically enhanced AMR and the associated pathogenicity, advancing our understanding of the risks posed by coexistence of multiple chemical pollutants in the environment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2025
·
IF 4.1
Zizhuo Li,Jinya Wei,Jitao Li,Yaling Yang,Dezhi Yang,Yuzhu Song
Herein, we rationally designed a novel and forceful ciprofloxacin (CIP) grafted zinc-doped-carbon dots (CIP@Zn/CDs) as an efficient artificial nanozyme against Escherichia coli ( E. coli ), multi-drug-resistant E. coli (AREC), and E. coli biofilms through the peroxidase (POD)-like catalytic activity and the synergistic antibacterial activity of CIP and Zn 2+ . On the one hand, extracellular DNA (eDNA) cleave by high levels of hydroxyl radicals (•OH) catalyzed by the POD-like catalytic activity of CIP@Zn/CDs. On the other hand, CIP@Zn/CDs with positive surface potential, elimination abilities against E. coli via electrostatic interaction, changing the cell membrane permeability and eventually leading to E. coli death within E. coli biofilms. Furthermore, the ultrasmall size of CIP@Zn/CDs possesses good biocompatibility favoring clinical translation. This work provides potential to develop CD-based techniques for applications in the field of selective antibacterial agents towards Gram-negative bacteria.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）