Analytical Methods
·
2024
·Vol. 16
·期 29
·4951-4959
·
IF 2.7
Weiqin Zhao,Ruichen Zhai,Qianxiao Chen,Chun Huang,Haojia Li,Youyu Zhu,Yingfeng Duan,Jie Gao
Glutathione (GSH) is commonly used as a diagnostic biomarker for many diseases. In this study, based on carbon quantum dots prepared from dragon fruit peel (D-CQDs) and the T-Hg(II)-T mismatch, a dual-mode biosensor was developed for the detection of GSH. This system consists of two single-stranded DNA (ssDNA). DNA1 was the T-rich sequence; DNA2 was attached to streptavidin-coated magnetic beads and consisted of T-rich and G-rich fragments. Due to the presence of Hg(II), the T-Hg(II)-T mismatch was formed between T-rich fragments of two ssDNA. In the presence of GSH, Hg(II) detached from dsDNA and bound with GSH to form a new complex. The G-rich fragment assembled with the hemin shed from D-CQDs to form the G-quadruplex/hemin complex. At this time, in fluorescence mode, the fluorescence of D-CQDs quenched by hemin could be restored. In colorimetric mode, after the magnetic beads separate, a visual signal could be produced by catalyzing the oxidation of ABTS using the peroxide-like activity of the G-quadruplex/hemin complex. This biosensor in both fluorescence mode and colorimetric mode had excellent selectivity and sensitivity, and the limit of detection was 0.089 µM and 0.26 µM for GSH, respectively. Moreover, the proposed dual-mode biosensor had good application prospects for detection of GSH.
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BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Jiaqi Chen,Mingzhi Li,Yanyao Cai,Yangzi Luo,Haodong Huang,Ruifeng Luo,Yuanyuan Ge,Zhili Li
Lignin has attracted attention in water treatment due to its extensive sources, complex structure, environmental friendliness, and functionality. Mannich modification is an effective method for enhancing the lignin's active sites, but it typically requires high temperatures and long reaction times, which limits its scalability for practical application. This study presents a simple, one-step Mannich reaction at room temperature for synthesizing aminated lignin (NAL) with high yield and ultra-high nitrogen content (17.34 %), using triethylenetetramine (TETA) as a modifier. The method effectively leverages TETA's symmetrical polyamine properties and glutaraldehyde (GDA)'s dual crosslinking functionality. Density functional theory (DFT) revealed that the potential and the discrepancy of reactive groups' energy gap (E gap ) were the key factors influencing the synthesis. The as-designed NAL exhibited excellent adsorption capacities of 1088.71 mg/g and 909.09 mg/g at 318 K for Hg(II) and Congo red (CR), respectively, that were superior to most reported modified lignin-based adsorbents. NAL also demonstrated robust resistance to ion interference, good reusability, and practical applicability. Notably, the adsorption performance of CR in the Hg(II)-CR binary system was enhanced with an adsorption capacity ratio (R q ) reaching 2.09. XPS, Zeta potential, and DFT calculations revealed that NAL's superior adsorption properties result from multiple interactions, including coordination, π-π interactions, hydrogen bonding, and electrostatic forces. Overall, NAL represents a green and highly effective material for environmental remediation with significant research and practical application value.
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ANALYTICAL LETTERS
·
2025
·
IF 1.8
Kong Weiheng,Zhu Haitao,Cheng Xingwang,Wang Yu,Song Wei,Zhou Dianbing,Fu Yan,Yu Xiaofeng,Wu Qiong,Han Fang
A functionalized Zr-based metal–organic framework (MOF) was synthesized using UiO-66 as the framework, polydiallyldimethylammonium (PDDA) as the interfacial modifier, and polydopamine (PDA) as the functionalizing agent through a straightforward one-pot hydrothermal reaction. The material underwent characterization through X-ray diffraction to determine its crystal structure, scanning electron microscopy and transmission electron microscopy to analyze its surface morphology and microstructure, and X-ray photoelectron spectroscopy to evaluate its elemental oxidation states. These analyses revealed that UiO-66-PDDA-PDA displayed well-dispersed PDA and an abundance of functionalized hydroxyl and amino groups. Utilizing this innovative functionalized MOF material, an electrochemical sensor was developed based on a UiO-66-PDDA-PDA-modified glassy carbon electrode for the simultaneous determination of Cd2+, Cu2+, Pb2+, and Hg2+. The fabricated electrochemical sensor exhibited excellent selectivity and rapid response to microgram levels of the mentioned heavy metal ions in tea, water, and dairy products using cyclic voltammetry. The sensor demonstrated remarkably low detection limits and stable relative standard deviations (RSDs) for the four heavy-metal ions. Furthermore, the electrochemical properties and applications of the modified electrode were investigated by optimizing operational parameters such as pH, deposition potential, and deposition time to improve the detection of trace ions. This approach offers a rapid screening method for inspectors to verify sample compliance.
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TALANTA
·
2025
·
IF 6.1
Shaowen Chen,Dongfei Chen,Yujie Shi,Renjie He,Xiaochan Wang,Gang Liu,Guo Zhao
Accurate detection of Hg(II) in soil is critical due to their severe environmental and heath risks. However, interference from soil organic matter, particularly humic acid (HA), complicates electrochemical detection by forming stable complexes with Hg(II), suppressing signal in square wave anodic stripping voltammetry (SWASV). This study introduces a novel pretreatment method using oxygen vacancy-rich ZnCo 2 O 4 nanocages (OVs-ZnCo 2 O 4 HNCs) to activate peroxymonosulfate (PMS) and generate sulfate radicals (·SO 4 − ). These radicals disrupt HA-Hg(II) complexes, restoring Hg(II) to its free, electroactive state for accurate detection. Optimized parameters, including ZnCo 2 O 4 loading (9 mg), PMS concentration (6 mg/L), and activation pH (3), enabled maximum signal recovery within 1.5 min. Comprehensive characterization confirmed the efficient catalytic activity of OVs-ZnCo 2 O 4 HNCs. The method demonstrated high sensitivity (LOD = 0.27 μg/L) and robustness in real soil samples, highlighting its potential for environmental monitoring and heavy metal analysis.
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ELECTROANALYSIS
·
2026
·Vol. 38
·期 1
·e70099
·
IF 2.3
Yang Chen,Xuening Wang,Huimin Si,Zhiping Sun,Qianyun Yu,Xihong Song,Ying Liu,Chuanbo Yan,Kai Yu
The increasing heavy metal pollution in marine environments has highlighted the urgent need for rapid, on-site detection methods. In this study, a reduced graphene oxide (rGO) and carboxylated multiwalled carbon nanotube (MWCNTs-COOH)-modified screen-printed electrode (SPE) was coupled with a portable electrochemical workstation for on-site detection of Pb 2+ and Cd 2+ in seawater. The key influencing factors involved in the detection process, such as deposition potential, deposition time, and pH value, have been systematically optimized. The response surface methodology (RSM) was applied to refine the portable electrochemical workstation parameters, including step potential, frequency, and pulse amplitude. The detection capability for heavy metals was significantly enhanced by using square wave stripping voltammetry (SWASV) with in situ Hg-Bi film plating, achieving detection limits of 0.4 μg/L for Pb 2+ and 0.8 μg/L for Cd 2+ . Finally, the portable workstation together with rGO/MWCNTs-COOH/SPE showed recovery rates of 101.33 ± 3.42% ~ 115.85 ± 3.50% for Pb 2+ and 101.32 ± 2.23% ~ 102.00 ± 2.00% for Cd 2+ in real seawater. The results were in good agreement with those obtained by inductively coupled plasma mass spectrometry (ICP-MS), thereby validating the detection accuracy of this platform.
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ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yihuan Song,Feng Guo,Pingxiu Zeng,Jinhua Liu,Yuanchao Wang,Heyong Cheng
Toxicity, mobility, bioavailability and biofunctions of chromium, cadmium, mercury and lead are heavily dependent upon their specific chemical forms, leading to a high demand to metal speciation analysis rather than total quantification. Simultaneous speciation analysis of multiple metal(loid)s is attractive to a large sample capacity containing unstable analytes due to its economic and environmental advantages over the conventional single elemental strategies. In this work, an analytical method integrating online solid phase extraction into high performance liquid chromatography interfaced with inductively coupled plasma mass spectrometry (ICP-MS) to simultaneously preconcentrate and quantify Cr, Cd, Hg and Pb forms in pg L −1 levels in water was developed. Cr(III + VI), Cd(II), Hg(II), Pb(II), methylmercury (MeHg), ethylmercury (EtHg), and trimethyl (TML) and triethyl lead (TEL) were captured by the C 18 adsorbent (equilibrated with 10 mL of 1.0 mM 2-hydroxyethanethiol at 10 mL min −1 ) and eluted by mobile phase (5.0 mM Cys at pH 2.0), then completely separated on the C 18 column within 8.0 min and eventually determined by ICP-MS. Low limits of detection (0.001–0.007 ng L −1 ) and quantification (0.003–0.023 ng L −1 ), good relative standard deviations (<4%) and high enrichment factors (827–2656 folds) were obtained with good linearities. Three reference materials of total cadmium (GBW08602), total mercury (GBW08603) and total lead (GBW08601) in water were analyzed by the developed method to validate the accuracy with good agreement with certified values and satisfactory recoveries (92–100%). This method was proved feasible by the determination of Cr, Cd, Hg and Pb compounds in drinking water , river water, pond water and tap water .
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DYES AND PIGMENTS
·
2022
·
IF 5.122
Changfang Lu,Yutong Wang,Wei Zhang,Yu Zhang,Yinyin Chen,Gang Xiang,Xianxiang Wang
The crosslinking of glutaraldehyde (GA) and nano-selenium coated chitosan (CS-SeNPs) afforded composites (GA–CS–SeNPs) which exhibit new properties. In addition to having mimic enzyme activity of CS-SeNPs, these composites exhibit fluorescence, which CS-SeNPs did not possess. The GA–CS–SeNPs can catalyze the chromogenic process of oxidation of substrate TMB at 20 °C and pH of 3.5, and under the excitation wavelength of 440 nm, the composites can emit strong fluorescence. The presence of mercury (II) (Hg 2+ ) inhibited the color process of catalytic oxidation of 3,3,5,5, -tetramethylbenzidine (TMB) and enhanced the fluorescence intensity of the composites to some extent. Therefore, using the fluorescence and mimicking enzyme properties of GA–CS–SeNPs, a fluorescent and colorimetric (dual-mode) determination of Hg 2+ was developed with good selectivity and high sensitivity. The linear determination ranges of Hg 2+ were 0–55 μM and 0–50 μM for fluorescent and colorimetric assays, respectively, and the limits of detection (LOD) for the dual-mode assay were as low as 0.34 μM and 0.40 μM, respectively. In addition, this new method was successfully implemented for dual-mode determination of Hg 2+ in real samples.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 2
·1-8
·
IF 5.705
Jian Yannan,Wang He,Lan Feifei,Liang Linlin,Ren Na,Liu Haiyun,Ge Shenguang,Yu Jinghua
An electrochemiluminescence (ECL) biosensor is described for the detection of microRNA (miRNA-155) based on tris(bipyridine)ruthenium(II) functionalized metal organic framework (RuMOF) materials. The material was prepared by a solvothermal method and was found to be stable even in acidic solution. However, it is selectively and sensitively disassembled by Hg(II) ions, resulting in the release of large quantities of Ru(II)(bpy) 3 ions, which produces a strong ECL signal. In view of the ion-selective disassembly and release and strand displacement process, an ultrasensitive ECL sensing method was established for detection of microRNAs. In the presence of the target, the hairpin structure of H1 can open and hybridize with the hairpin probe H2 to form a more stable H1-H2 duplex structure than the H1-target hybrid. The target of hybridization to H1 was immediately freed from the structure and the released target re-entered the new hairpin assembly target recovery process. The remaining H2 single fragment can bind to the I-RuMOFs-conjugates. The more hairpin probes H1, the more I-RuMOFs-conjugates load the DNA fragments, leading to the signal amplification. The method works in the 0.8 f. to 1.0 nM miRNA-155 concentration range and has a detection limit of 0.3 fM. The assay is sensitive, fairly specific and remarkably stable. In our perception, it offers an attractive tool for the sensitive detection of microRNAs in clinical samples.
An electrochemiluminescence (ECL) based biosensor is described for the detection of microRNA (miRNA-155) based on the use of a metal organic framework functionalized with ruthenium(II)tris(bipyridine) that was deposited on a glassy carbon electrode (GCE) modified with gold nanoparticles.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Junyang Wang,Caiyi Du,Peitong Yu,Qian Zhang,Hongxia Li,Chunyan Sun
A label-free and enzyme-free signal-on biosensor for Hg(II) ions was constructed based on T-Hg(II)-T nanoladders and fluorescent DNA-templated silver nanoclusters/graphene oxide nanocomposites (DNA-AgNCs/GO). The T-rich sequences (P1 and P2) were used to capture Hg(II) and form the T-Hg(II)-T nanoladders. C 6 G 5 C 6 was extended at the 5′ end of P1, used as a template for synthesizing fluorescent DNA-AgNCs. In the presence of mercury ions , the T-Hg(II)-T nanoladders significantly enhanced the emission of AgNCs. GO can attract free P1-C 6 G 5 C 6 and P2 that were not involved in the formation of T-Hg(II)-T nanoladders, thus employed as the energy acceptor to quench the background fluorescence of AgNCs. The fluorescence enhancement induced by mercury ions can be used for quantitative detection of Hg(II) over the range 0.1–30 nM with a detection limit as low as 7.35 pM. This method was used to detect Hg(II) in the spiked samples of Songhua River water and grass carp, with the recoveries of 97.61%− 103.79% and 96.52%− 105.58% respectively, consistent with the results from atomic fluorescence spectrometry. Obviously, in this assay, enzyme-free signal amplification and signal identification are integrated in the ingenious design of nucleic acid sequences, thus achieving the label-free fluorescence detection of mercury ions.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Chenglong Xue,Zhanpeng Ren,Jianying Wang,Minghua Deng,Jiashen Li,Xianbao Wang,Jinhua Li
The conventional methods for detecting Hg 2+ ions are generally impaired by low sensitivity, a high limit of detection (LOD), and the long detection time required. In this work, we develop a novel solution gated graphene transistor (SGGT) based on carbon dots doped with N and S elements (N/S CDs) as gate electrode probes. The prepared N/S CDs, which are 3–5 nm in diameter, are first modified at the gate of the SGGT through covalent bonding that specifically recognizes Hg 2+ ions. The SGGT is then used as a Hg 2+ sensor, exhibiting good selectivity, ultrahigh sensitivity, good reproducibility, and a short detection time. The SGGT sensor has an excellent linear detection range, from 0.1 fM to 1 nM. The LOD can reach 28 aM, which is far lower than that of conventional detection methods. Moreover, the SGGT sensor also has a rapid response time of ∼ 40 s. Finally, the ability to analyze real water samples and serum samples is demonstrated.
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Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Qin Tao,Tianwen Liu,Mengyao Si,Tingting Zhang,Qiu Jin,Xiaoying Wang
The simultaneous presence of heavy metal ions (HMIs) such as Pb²⁺ and Hg²⁺ pose severe risks to food safety, particularly in aquatic products. Herein, a ratiometric electrochemical aptasensor integrated with magnetic separation-assisted entropy driven catalyst (EDC) was developed for simultaneous detection of Pb²⁺ and Hg²⁺. In this work, Fe@ZIF67-NH₂, an iron-doped zeolitic imidazolate framework, as both an electrode modifier and an internal reference, providing stable electrochemical signals and enhanced surface area. Herein, we utilized Fe@ZIF67-NH₂ (an iron-doped zeolitic imidazolate framework) as both an electrode modifier and an internal reference to provide stable electrochemical signals and increase the surface area. Dual EDC reactions amplified Pb²⁺ and Hg²⁺ signals independently, while magnetic separation isolated carbon dots (CDs) labelled DNA strands (CDP-D1 and CDH-P1) from interference strands, minimizing background noise. The sensor demonstrated wide linear ranges of 0.2-2.0×10⁴ ng mL⁻ 1 for Pb²⁺ and 0.1-1.0×10⁴ ng mL⁻ 1 for Hg²⁺, with detection limits of 0.1 ng mL⁻ 1 and 0.03 ng mL⁻ 1 , respectively. Practical validation using dried seafood samples showed recovery rates of 96.4-107.0% and relative standard deviations (RSDs) < 6.60%, and were consistent with inductively coupled plasma mass spectrometry (ICP-MS). This work presents a highly amplification-efficient and sensitive strategy for multiplex HMI detection in complex matrices, offering significant potential for food safety monitoring and environmental analysis.
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JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 11.3
Pingxiu Zeng,Zhenzhen Ma,Hongmei Hu,Meiying Ye,Yuanchao Wang,Heyong Cheng
Species-specific toxic, metabolic, and bioavailable properties of commonly coexisting metal(loid)s—particularly Cr, As, Cd, Hg, and Pb—necessitate their speciation analysis. Because of the complexity and diversity in chemical form, concentration (from picograms to milligrams per liter), interactions, and sample matrix, multi-elemental speciation methods are preferred over traditional mono-elemental speciation methods. However, current multi-elemental speciation methods have primarily associated with only two metal(loid)s. Herein, we developed an efficient, sensitive, inexpensive, and green methodology for the simultaneous penta-elemental speciation analysis of Cr, As, Cd, Hg, and Pb using high-performance liquid chromatography combined with inductively coupled plasma mass spectrometry and online solid-phase extraction (SPE) preconcentration. Food samples were extracted with a mixture of 0.1 M HNO 3 and 5 mM L -cysteine (Cys), chosen among 10 extractants for its good extraction efficiency, low noise, and minimal chromatographic interference. The proposed method offered a short analysis time (10 min for separation and 2 min for enrichment), excellent linearity (R 2 > 0.995), low detection limits (0.02 −0.12 μg L −1 in SPE-free mode; 0.02 −3.2 ng L −1 with online SPE), high precision (0.2 −2.4 %), and good recovery (81 −117 %) using an aqueous mobile phase of 5 mM tetrabutylammonium hydroxide + 5 mM Cys (pH 4.7). This demonstrates its good applicability to both food and water samples. Despite the incompatibility of Cr(VI) with As(III) and Cys, the proposed method excels in analytical, economic, and environmental aspects (maximizing the number of metal(loid)s within minimized analytical time using aqueous mobile phases), showing significant potential for a wide range of food, environmental, and biological samples.
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