MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Meixin Li,Pengfei Yu,Shanshan Li,Mati Ullah Khan,Shun Wang,Jiaqi Tian,Shuang Liu,Ling Kong,Zhiwei Chen
Aflatoxin B 1 (AFB 1 ) and ochratoxin A (OTA) are highly toxic mycotoxins prevalent in food and feed, posing severe threats to human and animal health. This work presents an innovative photoelectrochemical aptamer sensor for the detection of AFB 1 and OTA, leveraging a ternary BiOBr/Bi/BiOI heterojunction and Ag 2 S quantum dot-mediated signal amplification. The BiOBr/Bi/BiOI composite was synthesized via a one-step hydrothermal reduction using l -ascorbic acid (AA), exhibiting a high specific surface area to enhance biorecognition efficiency. In situ-assembled Ag 2 S quantum dots amplified photocurrent responses, while a single aptamer probe enabled specific dual-target recognition. Under optimized conditions, the sensor achieved exceptional sensitivity: linear ranges of 0.001–100 ng·mL −1 for both mycotoxins, with detection limits of 4.34 fg·mL −1 (AFB 1 ) and 13.54 fg·mL −1 (OTA). Validation in complex food matrices confirmed high accuracy and robustness. This platform not only provides a reliable method for mycotoxin monitoring but also establishes a versatile design paradigm for multiplexed detection of food contaminants.
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高影响因子
FOOD CONTROL
·
2022
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IF 6.652
Chengyuan Wu,Xiangchuan Wu,Fan Hou,Lina Wu,Guangxia Liu
An ultrasensitive electrochemical aptasensor was established based on metalloporphyrin metal organic frameworks ( [email protected] ) with peroxidase-like activity and exonuclease III-assisted recycling magnification for the detection of ochratoxin A (OTA). [email protected] showed well peroxidase-like activities due to Fe-tetrakis (4-carboxyphenyl) porphyrin (Fe-TCPP) as a heme-like ligand and in situ supported with palladium nanoparticle, which could be used for the label of ssDNA instead of nature enzyme. The designed exonuclease III-assisted recycling magnification further improve the sensitivity for the detection. Based on this, the proposed aptasensor showed a well linear relationship within the scope of 1 × 10 −5 ng mL −1 to 10 ng mL −1 and a low detection limit of 6.79 fg mL −1 . Additionally, the application in the real spiked oil samples confirmed the dependability and veracity of the aptasensor. Hence, the designed aptasensor has comprehensive application prospects for the detection of OTA in food .
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高影响因子
MOLECULES
·
2023
·Vol. 28
·期 15
·5743
·
IF 4.6
Yexuan Ren,Ruwen Tian,Ting Wang,Junlin Cao,Jianguo Li,Anping Deng
In this study, an extremely highly sensitive enzyme-linked immunosorbent assay (ELISA) based on a newly produced monoclonal antibody (mAb) for the detection of ochratoxin A (OTA) in food samples was developed. OTA-Bovine serum albumin (BSA) conjugate was prepared and used as the immunogen for the production of the mAb. Among four hybridoma clones (8B10, 5C2, 9B7, and 5E11), the antibody from 8B10 displayed the highest affinity recognition for OTA. Based on the mAb (8B10), the IC50and LOD of the ELISA for OTA were 34.8 pg mL−1and 1.5 pg mL−1, respectively, which was 1.53~147 times lower than those in published ELISAs, indicating the ultra-sensitivity of our assay. There was no cross-reactivity of the mAb with the other four mycotoxins (AFB1, ZEN, DON, and T-2). Due to the high similarity in molecular structures among OTA, ochratoxin B (OTB), and ochratoxin C (OTC), the CR values of the mAb with OTB and OTC were 96.67% and 22.02%, respectively. Taking this advantage, the ELISA may be able to evaluate total ochratoxin levels in food samples. The recoveries of the ELISA for OTA in spiked samples (corn, wheat, and feed) were 96.5–110.8%, 89.5–94.4%, and 91.8–113.3%; and the RSDs were 5.2–13.6%, 8.2–13.0%, and 7.7–13.7% (n= 3), respectively. The spiked food samples (corn) were measured by ELISA and HPLC-FLD simultaneously. A good correlation between ELISA (x) and HPLC-FLD (y) with the linear regression equation of y = 0.918x − 0.034 (R2= 0.985,n= 5) was obtained. These results demonstrated that the newly produced mAb-based ELISA was a feasible and ultra-sensitive analytical method for the detection of OTA in food samples.Keywords:enzyme-linked immunosorbent assay (ELISA);monoclonal antibody (mAb);mycotoxin;ochratoxin A (OTA);food samples;sensitive detection
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PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 21
·5210-5217
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IF 3.864
Jinxin Chi,Maolin Chen,Lijun Deng,Xucong Lin,Zenghong Xie
A convenient and high-performance AuNPs@aptamer-modified mercaptosiloxane-based hybrid affinity monolithic column with an unusually high coverage density of aptamers was facilely prepared and used for on-column selective recognition of ochratoxin A (OTA). Due to the high surface-to-volume ratio of AuNPs, the robust conjugation of Au-SH and large specific surface area of hybrid-silica monolith, high coverage density of 5′-SH-aptamers up to 3494 pmol μL−1 was achieved, which was 2.5–10 folds higher than that of other previously reported affinity monoliths modified with AuNPs@Apt. Using OTA as the model analyte, the highly selective recognition of OTA was carried out via online coupling with HPLC, and the cross-reactivity towards analogues, such as OTB and aflatoxin B1, was weak. High recovery yields of OTA were achieved at more than 92% (n = 3) even when OTB was added at a high concentration level up to 50 ng mL−1. For sample analysis, efficient discrimination of OTA was successfully obtained with a sensitive detection limit of 25 pg mL−1. The recoveries of OTA with different fortified levels were achieved at 88.6%–94.1% and 88.2%–94.3% for beer and wine samples, respectively. This protocol provides a facile approach for fabricating a desirable affinity monolith modified with abundant aptamers for highly selective and sensitive on-column extraction of target analyte OTA.
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高影响因子
CHEMOSPHERE
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2025
·
IF 8.1
Si-yuan He,Wei Wen,Peng Wu,Zhe Meng
Developing a green and efficient technology for decomposing aflatoxin B 1 (AFB 1 ) and ochratoxin A (OTA) in grains has important theoretical value and application prospects for ensuring food safety. A dual Z-scheme ternary heterojunction photocatalyst, Sr 2 MgSi 2 O 7 :Eu 2+ ,Dy 3+ /BiVO 4 /NH 2 -UiO-66 (SBN), was prepared via a self-assembling hydrothermal approach. The SBN heterojunction photocatalyst exhibits round-the-clock photocatalytic activity due to the intrinsic light source of Sr 2 MgSi 2 O 7 : Eu 2+ , Dy 3+ (SMS). Dual Z-scheme mechanism was proposed and validated through detailed analysis of quenching experiments, ESR spectra, band arrangement, and charge transfer processes. The dual Z-scheme mechanism not only promotes the effective transfer and separation of photo generated charge carriers during charge carrier transport, but also maintains significant reduction and oxidation potentials. SBN photocatalyst exhibits stable and persistent photocatalytic performance, significantly enhancing the degradation efficiency of AFB 1 and OTA under all-weather conditions. Experimental data shows that the removal rate of AFB 1 increased from 69.60 % after 60 min of illumination to 83.9 % after 4 h of turning off the light source; At the same time, the removal rate of OTA increased from 64.8 % to 78.1 %. •O 2 − and •OH were identified as key active species in photocatalytic reactions. Based on the analysis of AFB 1 and OTA degradation intermediates and the prediction of degradation sites using density functional theory (DFT), degradation pathways for AFB 1 and OTA were proposed. Collectively, these findings suggest a highly promising practical approach for the detoxification of mycotoxins in grain commodities.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Hongshuai Zhu,Yue Cai,Aori Qileng,Zhu Quan,Wei Zeng,Kaiyu He,Yingju Liu
The convenient and effective detection of toxins is urgently demanded for food security and human health. Herein, based on the catalytic activity of mimetic peroxidase from the Cu 2 [email protected] (OH) 3 yolk-shell nanocages, a dual-modal multi-colorimetric and ratiometric fluorescence immunosensor for the sensitive detection of ochratoxin A (OTA) was successfully developed. For the multi-colorimetric detection, H 2 O 2 can be effectively decomposed by Cu 2 [email protected] (OH) 3 to form ·OH groups, thus Au nanorods (Au NRs) can be etched to exhibit vivid color variations and localized surface plasmon resonance (LSPR) shifts. For the ratiometric fluorescence detection, o -phenylenediamine was oxidized by Cu 2 [email protected] (OH) 3 to form 2,3-diaminophenazine (DAP) in the presence of H 2 O 2 . Interestingly, the exogenous fluorescence signal source of carbon dots can be quenched by DAP via inner filter effect, while a new emission peak at 563 nm can be discovered, forming a ratiometric fluorescence signal. Due to the independent signals and mutual confirmation, the performance of the dual-modal immunosensor for the detection of OTA was significantly improved, where a broad linear range from 1 ng/L to 10 μg/L with a detection limit of 0.56 ng/L (S/N = 3) was achieved. The sensing strategy was also used to monitor OTA in millet and lake water samples with a satisfied performance.
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高影响因子
TALANTA
·
2024
·
IF 5.6
Qi Liu,Zongyi Li,Caifeng Zou,Shi Feng,Juncheng Song,Xiangyang Li
Timely and accurate on-site detection of ochratoxin A (OTA) is extremely important for global public health. In this study, a fluorescence/colorimetric biosensor based on Ti 3 C 2 nano-materials (Ti 3 C 2 -NMS) and a machine-learning (ML) based fluorescence/colorimetric intelligent learning system for detection of OTA concentration (COTA) were developed. The sensor was fabricated by functionalizing Ti 3 C 2 -NMS prepared by physical-exfoliation assisted metal-ion-induction using ssDNA. The Ti 3 C 2 -NMS exhibited good fluorescence quenching characteristics (FQC) and peroxidase-like activity (PLA). More surprisingly, the functionalization of Ti 3 C 2 -NMS by ssDNA further enhanced the FQC and PLA of the material, which could be used for dual-mode detection of OTA. When different COTA existed, ssDNA competitively bound to OTA, resulting in regular changes in fluorescence and colorimetric signals of the sensor, which realized the accurate and sensitive biosensing detection of OTA in two modalities. Based on a series of fluorescent/colorimetric RGB datasets collected by a self-developed application, a dual-channel ML model had been developed. This model can be integrated into mobile phones, clouds, and PCs to achieve intelligent sensing detection of OTA with the assistance of fully connected artificial neural networks. The method constructed had high specificity, low cost, and fast responsiveness, with a LOD as low as 1.58 pg mL −1 , indicating excellent potential for application and promotion.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Zhenyun He,Fujing Mao,Zhichang Sun,Qian Yang,Di Huang,Xing Liu
Rapid and sensitive monitoring of ochratoxin A (OTA) in agricultural products can minimize its threats to public health. This study introduces a ratiometric fluorescence nanosensor-driven dual-mode enzyme immunoassay (RFN-DEIA) for fluorescent and visual detection of OTA. Herein, a bifunctional mimotope peptide-alkaline phosphatase fusion protein (MP-ALP) was utilized to compete for binding to the OTA-specific nanobody and dephosphorylate ascorbyl 2-phosphate. The connection between the MP-ALP-based immunoassay for OTA and the 9-anthraldehyde-based ratiometric fluorescence nanosensor for MP-ALP was facilitated using MnO 2 nanosheets. The optimized RFN-DEIA has a visual limit of detection (LOD) of 2.75 ng/mL and an instrumental LOD of 1.50 ng/mL. It exhibits high selectivity for OTA, and good recoveries of 80.66%–117.18% with relative standard deviations not exceeding 9.5% in recovery experiments. Furthermore, the RFN-DEIA was applied to detect OTA in real pepper samples and validated by high-performance liquid chromatography, demonstrating its effectiveness and reliability for OTA detection in pepper samples.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
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IF 4.9
Chenxi Ke,Yu Wu,Jiayao Ou,Hongda Zhu,Huiling Guo,Hongmei Sun,Mingxing Liu
Ochratoxin A (OTA) is a representative foodborne fungal toxin that poses great harm to humans, the ultra-sensitive detection of OTA is an urgent requirement for public health. In this work, a competitive colorimetric and ratio fluorescence dual-mode immunosensor for the detection of OTA based on 0D/2D AuAg NCs/SPCN/BGQDs heterojunction nanozyme was devised and fabricated. The presence of P-N heterojunctions was confirmed through electrochemistry and theoretical calculations, heterojunction formed between AuAg NCs-SPCN and BGQDs effectively augmented the oxidase-mimic activity, verified by enzyme kinetic analysis. Superoxide radicals (·O2-) were catalyzed by heterojunction nanozyme from dissolved oxygen, and o-phenylenediamine was oxidized by ·O2- to produce diaminophenazine. It exhibited an absorption peak at 450 nm overlapped with the emission peak of heterojunction nanozyme. The fluorescence at 434 nm was quenched through the IFE effect while the fluorescence at 565 nm was enhanced and accompanied by solution yellowing, so ratio fluorescence was formed due to two emission peaks and the dual-mode detection system was constructed. The dual-mode immunosensor exhibited exemplary linearity across the concentration span of 0.001–20 μg/L, with LODs of 0.28 ng/L (ratio fluorescence) and 0.2 ng/L (colorimetry) respectively. Therefore, this immunosensor demonstrated potential applications in food quality testing and visual analysis.
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高影响因子
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