BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Xiaolan Li,Yufei Zhang,Hossain M. Zabed,Junhua Yun,Guoyan Zhang,Mei Zhao,Yuvaraj Ravikumar,Xianghui Qi
d -Arabitol is a top value-added compound with wide applications in the food, pharmaceutical and biochemical industries. Nevertheless, sustainable biosynthesis of d -arabitol is limited by lack of efficient strains and suitable fermentation process. Herein, metabolic engineering and process optimization were performed in Zygosaccharomyces rouxii to overcoming these limitations. Adopting systems metabolic engineering include enhancement of innate biosynthetic pathway, supply of precursor substrate d -ribulose-5P and cofactors regeneration, a novel recombinant strain ZR-5A with good performance was obtained, which boosted d -arabitol production up to 29.01 g/L, 59.31 % higher than the parent strain. Further with the optimum medium composition and fed-batch fermentation, the strain ZR-5A finally produced 149.10 g/L d -arabitol with the productivity of 1.04 g/L/h, which was the highest titer ever reported by Z.rouxii system. This is the first report on the use of metabolic engineering to construct Z. rouxii chassis for the sustainable production of d -arabitol.
研究领域:
生物技术
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代谢工程
·
发酵技术
·
生物化工
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产品关联
DOI
PubMed
高影响因子
Experimental and Therapeutic Medicine
·
2023
·Vol. 26
·期 2
·1-15
·
IF 2.7
Jianxin Wang,Peng Guan,Yu Chen,Meng Xu,Na Wang,Ensheng Ji
Myocardial dysfunction is a frequent complication in patients with severe sepsis. However, effective drugs for the prevention of myocardial dysfunction and the molecular mechanisms of the disease remain elusive. The present study demonstrated that Cyclovirobuxine D (CVB‑D) could improve cardiac dysfunction in a cecal ligation and puncture (CLP) model in rodents and in a lipopolysaccharide (LPS) model in vitro. Echocardiography and histopathological examination were used to detect changes in cardiac structure and function. Kits were used to detect indicators of cardiac injury, transmission electron microscopy to detect structural changes in mitochondria and reverse transcription‑quantitative PCR to detect prostaglandin‑endoperoxide synthase 2 and hamp expression levels. L‑Glutathione and malondialdehyde levels and superoxide dismutase activity were measured using kits. Cell viability was measured with the Cell Counting Kit‑8. Iron metabolism‑related proteins, inflammatory factor levels and related pathway proteins were detected using western blot analysis. Changes in L‑type calcium currents were detected by membrane clamp, and contractility of cardiomyocytes was measured by Ion Optix. CVB‑D attenuated CLP‑induced cardiac malfunction in septic rats, with changes observed in myocardial pathological structure, creatine kinase isoenzyme (CK‑MB), lactate dehydrogenase (LDH) and cardiac troponin I (cTnI). CVB‑D attenuated sepsis‑induced lipid peroxidation and iron overload. In addition, CVB‑D decreased the expression of CK‑MB, LDH and cTnI, suppressed oxidative stress index levels and reduced the production of reactive oxygen species. CVB‑D decreased LPS‑induced cytoplasmic iron overload by increasing upregulation of iron uptake molecules. Conversely, CVB‑D significantly increased the upregulation of ferroportin 1. CVB‑D pretreatment significantly reduced the levels of hamp mRNA compared with the LPS‑treated group. CVB‑D pretreatment significantly reduced inflammatory factor levels and the ratio of phosphorylated vs. total signal transducer and activator of transcription 3. The expression of SLC7A11 and GPX4 was upregulated in septic cells pretreated with CVB‑D, however treatment with ML385 largely decreased this upregulation. Of note, CVB‑D inhibited the inward flow of calcium ions through the LTCC. In conclusion, these findings suggest that CVB‑D alleviated sepsis‑induced cardiac iron toxicity by alleviating iron metabolism.
研究领域:
药理学
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心脏病学
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免疫学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Neural Regeneration Research
·
2020
·
IF 3.171
Jian-Zhu Bo,Ling Xue,Shuang Li,Jing-Wen Yin,Zheng-Yao Li,Xi Wang,Jun-Feng Wang,Yan-Shu Zhang
Although exogenous D-serine has been applied as a neural regulatory intervention in many studies, the role played by D-serine in hippocampal injuries caused by lead exposure remains poorly understood. Rat models of chronic lead exposure were established through the administration of 0.05% lead acetate for 8 weeks. Simultaneously, rats were administered 30 or 60 mg/kg D-serine, intraperitoneally, twice a day. Our results showed that D-serine treatment shortened the escape latency from the Morris water maze, increased the number of times that mice crossed the original platform location, and alleviated the pathological damage experienced by hippocampal neurons in response to lead exposure. Although D-serine administration did not increase the expression levels of the N-methyl-D-aspartate receptor subtype 2B (NR2B) in the hippocampi of lead-exposed rats, 60 mg/kg D-serine treatment restored the expression levels of NR2A, which are reduced by lead exposure. These findings suggested that D-serine can alleviate learning and memory impairments induced by lead exposure and that the underlying mechanism is associated with the increased expression of NR2A in the hippocampus. This study was approved by the Animal Ethics Committee of North China University of Science and Technology, China (approval No. LX2018155) on December 21, 2018.
研究领域:
神经科学
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毒理学
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药理学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 43
·6131-6138
·
IF 1.9
Xiangyu Wang,Huihui Wu,Rongying Luo,Donghai Xia,Zhengjin Jiang,Hai Han
D-amino acids are currently paid attention to as new physiologically active substances. Foodstuffs and beverages containing D-amino acids are a matter of interest. Until now, the profiles of D-amino acids have not been reported in natural and fermented teas except theanine. In this study, an off-line 2D-HPLC method combining a Gemini C18 column and a CHIRALPAK® IC-3 column or a self-prepared poly(MQD-co-HEMA-co-EDMA) monolithic capillary column was employed for the separation and detection of D-amino acids in tea samples with the co-existence of a large amount of L-amino acids. The free amino acid fractions in longjing, black, oolong, and pu-erh tea samples were separated and collected after pre-column derivatization using 9-fluorenylmethoxycarbonyl (FMOC) chloride in the reversed-phase mode, and then were concentrated and separated to D- and L-forms on a chiral column. Among them, the D-form of isoleucine (Ile) (1.0–1.6%), alanine (Ala) (0.1–0.8%), phenylalanine (Phe) (0.1–0.4%), valine (Val) (0.2–0.3%), serine (Ser) (0.1–0.2%), aspartic acid (Asp) (0.2%), and proline (Pro) (0.1%) were detected in longjing and oolong teas. Differences between natural tea and fermented tea in the profiles of D-amino acids as well as the total amino acids were also observed. The results provided useful information for the bio-function research of D-amino acids in tea.
研究领域:
食品科学
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分析化学
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生物化学
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产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Ranran Wei,Xiaoying Zhang,Min Yan,Yaxuan Li,Ruichen Geng,Zekai Shen,Yinglong Wang,Shuli Yin
Ammonia (NH3), the second most produced chemical in the world, is predominantly utilized in fertilizer production to underpin modern agriculture and has recently attracted interest as an energy-dense source of renewable energy. Herein, an amorphous CuTe porous nanonets (CuTe PNs) catalyst with p-d orbital hybridization was designed and prepared for NH3 synthesis via the nitrite reduction reaction. The porous and amorphous structure of CuTe PNs provides sufficient channels for molecular penetration and enhances active site accessibility through its abundant defects and vacancies, facilitating electrochemical reactions. The CuTe PNs exhibit excellent nitrite reduction reaction performance (NH3 yield: 633.7 μg h–1 mgcat–1, Faradaic efficiency: 91.41%, NH3 selectivity: 92.2%). This work describes a powerful pathway for the fabrication of p-d orbital hybrid amorphous porous nanonets catalysts and also provides a new possibility for the electrochemical synthesis of NH3.
研究领域:
材料科学
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电化学
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催化化学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Jiaxin Liu,Keke Ning,Yao Fu,Yujie Sun,Jiangong Liang
N-acetyl-beta-D-glucosaminidase (NAG) is an important biomarker for early clinical diagnosis of renal disease, suggesting the necessity to develop a fast and sensitive method for its detection. In this paper, we developed a fluorescent sensor based on polyethylene glycol (4 0 0) (PEG-400)-modified and H 2 O 2 -assisted etched sulfur quantum dots (SQDs). According to the fluorescence inner filter effect (IFE), the fluorescence of SQDs can be quenched by the p-nitrophenol (PNP) generated by NAG-catalyzed hydrolysis of p-Nitrophenyl-N-acetyl-β-D-glucosaminide (PNP-NAG). We successfully used the SQDs as a nano-fluorescent probe to detect the NAG activity from 0.4 to 7.5 U·L −1 , with a detection limit of 0.1 U·L −1 . Furthermore, the method is highly selective and was successfully used in the detection of NAG activity in bovine serum samples, suggesting its great application prospect in clinical detection.
研究领域:
生物化学
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医学检测
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纳米技术
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Feng Du,Zhikun Yao,Jikai Xiang,Jingsha Li,Changhong Wang,Chunmei Zhang,Tao Hu,Jinlong Liu,Changming Li,Chunxian Guo
To design high-performance electrocatalysts with desired electronic structures is critical to facilitate the conversion of pollutant nitrate (NO 3 − ) to value-added ammonia (NH 3 ) at ambient condition. Nevertheless, the current electrocatalysts for NO 3 − -to-NH 3 still face low Faradaic efficiency and selectivity. We present here a heteroatoms co-doped strategy using phosphorus (P) and oxygen (O) to tune electronic structure of copper (Cu) nanocrystals to form highly active surface for selective NO 3 − -to-NH 3 . By combing experimental results and theoretical calculation, it is found that the heteroatom P, O-doping can negatively shift the Cu d -band center, which optimizes adsorption energies of intermediates, especially for *NO 3 − and *NH 3 . The P, O-doped Cu nanocrystal exhibits an efficient 8-electron transfer process for NO 3 − -to-NH 3 . It achieves both high NO 3 − conversion rate (91.09 %) and NH 3 Faradaic efficiency (91.72 %) in high concentration NO 3 − electrolyte (1.0 M KOH + 1400 ppm NO 3 − -N), retaining its potential applications in practical NO 3 − -to-NH 3 .
研究领域:
材料科学
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电催化
·
环境科学
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DOI
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2025
·Vol. 39
·期 2
·e7980
·
IF 0
Yong Yang,Wei He,Dengmeng Song,Fang Xie,Jiaying Li,Jun Li,Ning Wang
A D-A 1 -A 2 type covalent triazine framework (CTF) was prepared for the photocatalytic hydrogen evolution (HER). In the resulting CTF-CZ-Bpy 2+ , 9-ethyl-9 H -carbazole (CZ) group is used as the donor, triazine ring as the first acceptor, and cyclic diquat (Bpy 2+ ) moiety as the second acceptor. Both experimental and theoretical results show that the D-A 1 -A 2 structure significantly enhances the efficiency of photogenerated charge separation and transfer. As a result, CTF-CZ-Bpy 2+ demonstrated outstanding photocatalytic HER activity with a yield rate of 31.1 mmol g −1 h −1 , which is 7.8 times higher than that of nonfunctionalized CTF-CZ-Bpy.
研究领域:
材料科学
·
能源化学
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产品关联
DOI
SYNTHETIC METALS
·
2013
·
IF 2.109
Wang Gang,Tan Haijun,Zhang Yiping,Wu Yingying,Hu Zhubin,Yu Guipeng,Pan Chunyue
Novel D-π-A system organic dyes (ID1, ID2 and ID3) based on isoindigo (ID), which contain triphenylamine (ID1) or isoindigo (ID2 and ID3) as electron donors, isoindigo and thiophene as a π-conjugated system and a cyanoacrylic acid moiety as an electron acceptor and anchoring group, have been then synthesized and characterized by 1 H NMR, UV–vis, CV. Dye-sensitized solar cells (DSSCs) based on these dyes were also fabricated and tested. As the photovoltaic performance tests demonstrated, the ID1 exhibits broader absorption spectra , higher maximum incident photon-to-current conversion efficiency (IPCE) and maximum photon-to-electron conversion efficiency ( η ) of 3.33% under 100 mW/cm 2 simulated AM 1.5G solar irradiation.
研究领域:
材料科学
·
能源科学
·
有机化学
✓ 已验证
产品关联
DOI
高影响因子
Molecular Catalysis
·
2021
·
IF 5.062
Teng Li,Guangyu Sun,Lu Xiong,Boying Zheng,Yaqi Duan,Rui Yu,Jun Jiang,Yantao Wang,Weiran Yang
As a useful chemical, furan can be produced from bio-based chemicals, such as furfural and furoic acid (FA). There were few reports on the preparation of furan from bio-based C6 aldaric acids . Herein, a new method was developed to produce furan from D-glucaric acid (GA) without transition metals, and nearly 80% yield of furan was produced in the water/toluene biphasic system with SnCl 4 at 200 °C in 2 h. 3-Hydroxy-2-pyrone (3-HP) and FA were proved to be the key intermediates in the formation of furan from GA, and the reaction pathway was proposed to be GA→ 3-HP→ FA→ furan based on the kinetic study , model substrate reaction, and isotope labeling experiments. SnCl 4 gave the highest furan yield among the common acid catalysts, and the contrast experiments suggested that H + , Cl − and Sn 4+ all have promoting effect on GA conversion to furan. This method was also applicable with other C6 aldaric acids.
研究领域:
生物化学
·
有机化学
✓ 已验证
产品关联
DOI
高影响因子
Membranes
·
2021
·Vol. 11
·期 8
·612
·
IF 4.106
Zhan Gao,Zhihao Yu,Xiaoli Zhang,Shougang Fan,Huiyu Gao,Caini Liu,Qixing Zhou,Huaiqi Shao,Lan Wang,Xiaoyan Guo
The thorny issue of membrane biofouling in membrane bioreactors (MBR) calls for new effective control measures. Herein, D-amino acid (DAA) was employed to mediate MBR membrane biofouling by inhibiting biofilm information and disintegrating formed biofilm. Different DAA control ways involving membrane property, DAA-adding timing, and DAA-control mode were explored through experiments and the multiple linear regression model and the response surface methodology. The optimized DAA control ways were acquired, involving DAA used as an active agent, and the DAA-adding timing of 4 h cultured before running, as well as both hydrophilic and hydrophobic membrane, resulting in an approximately 40.24% decrease in the membrane biofouling rate in comparison with the conventional MBR. DAA is an efficient membrane biofouling mediating approach for MBR under optimized control ways combination and a facile solution for solving membrane biofouling in actual membrane systems.Keywords:membrane bioreactor;membrane biofouling;D-amino acid;control way;model evaluation
研究领域:
环境工程
·
微生物学
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产品关联
DOI
PubMed
高影响因子
ACS Catalysis
·
2022
·
IF 13.7
Shuangxi Lin,Qiang Wang,Maoshuai Li,Ziwen Hao,Yutong Pan,Xiaoyu Han,Xiao Chang,Shouying Huang,Zhenhua Li,Xinbin Ma
Controlling the selectivity of CO2 hydrogenation by catalysis is a fundamental challenge. This study examines the interrelation between active sites and reaction pathways in Ni-catalyzed CO2 hydrogenation. The alloying of Ni with Zn to charged (Niσ––Znσ+) active sites modifies the electronic structure and d-band center, weakens the interaction with CO/H2, and preferentially catalyzes the reverse water gas shift to CO with the thermodynamically favored methanation pathway switched off. The charged dual sites can stabilize the activated CO2 species in a η2(C, O) bridge configuration, directly dissociate the C═O bond to *CO, and promote CO desorption. The mechanistic investigation has elucidated the reaction pathways in the Ni-catalyzed CO2 hydrogenation and identified the crucial intermediates that impacted the product selectivity, which can provide a theoretical guide for the Ni-based catalyst design.
研究领域:
催化化学
·
材料科学
·
表面科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）