PROCESS BIOCHEMISTRY
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2017
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IF 2.497
Jinsha Huang,Wei Zhuang,Ce Wei,Liwen Mu,Jiahua Zhu,Yudan Zhu,Jinglan Wu,Yong Chen,Hanjie Ying
Orientation immobilization of enzymes has attracted intensive interest owing to the retainable specific activity and stability. Specially, glycoprotein immobilized onto Concanavalin A (Con A) modified carriers induces the orientation of the enzyme. However, the effects of the interface properties of carriers and enzymes are still not well understood yet. In this study, we synthesized the activated porous poly (styrene- divinylbenzene) resin carriers with 30 nm pore sizes and 72 m 2 g −1 specific surface areas and decorated with Con A. The resultant loading capacity of NP 1 on Con A modified carriers was as high as 4.02 mg g −1 wet support as a result of strong affinity between the enzyme and Con A decorated on carriers. It was found that the acid resistance, thermal stability, reusability and degradation efficiency of the immobilized enzyme on Con A modified porous carriers were significantly improved. The reduction of K m from 18.40 ± 0.55 mg mL −1 to 17.19 ± 0.51 mg mL −1 illustrated the improved substrate affinity of HA-GA-ConA-NP 1 . Moreover, Con A-affinity NP 1 exhibited the best operational stability that only 7% of its initial activity was lost even after 9 batches repeated reaction. This work demonstrates that surface property manipulation of porous carriers and its derivatives has great potential in efficient biocatalytic systems.
研究领域:
生物化学
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酶工程
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DOI
高影响因子
JOURNAL OF FLUORESCENCE
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2019
·Vol. 29
·期 6
·1285-1290
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IF 1.913
Wen Qin,Liu Wanqiang,Zheng Yuhui,Gao Jinwei
In this study, the bio-inspired self-cracking substrate can be employed as a template to control the formation of a robust mirco-patterning film. Uniform materials with variable diameters are achieved by optimizing the egg white/water ratios and reaction temperature. The incorporated europium ions exhibit its typical f-f transitions in patterned films. Moreover, the mechanical property and excellent transmittance features have been demonstrated. The current approach can be extended to design two-dimensional nanofabrication of different materials.
研究领域:
材料科学
·
光学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Sidra Naseer,Jie Ouyang,Xing Chen,Shujin Pu,Yanting Guo,Xuan Zhang,Dali Li,Chengli Yang
Nanoparticles have been successfully used for immobilization of different enzymes . The enzyme immobilized by nanomaterials has promising application in the biotechnological industry. The β-glucosidase is very important in industrial field. The immobilization of β-glucosidase by nanomaterials increases its activity and reusability. In this work, the β-glucosidase was extracted from fruiting bodies of Agrocybe aegirit and was purified by anion exchange . The β-glucosidase was immobilized by SiO 2 nanoparticles (nano-SiO 2 ) crosslink with glutaraldehyde (GA). On the other hand, the β-glucosidase firstly immobilized by process adsorption and then crosslink with genipin (GP) which was produced by the hydrolysis of geniposide by β-glucosidase. Then we compared the properties of both immobilized GA-crosslinked β-glucosidase and GP-crosslinked β-glucosidase, their immobilization yields were 83.34% and 96.29% respectively. The GA-crosslinked β-glucosidase and free β-glucosidase revealed optimal pH at 6.0. The optimal pH of GP-crosslinked β-glucosidase was ranging between 4.5 and 7.5. The GA-crosslinked β-glucosidase and GP-crosslinked β-glucosidase revealed the optimal temperature at 50 °C, 70 °C respectively. For the free β-glucosidase, the optimal temperature was 55 °C. Furthermore, the GA-crosslinked β-glucosidase and GP-crosslinked β-glucosidase were characterized by scanning electron microscope (SEM). The geniposide consumption in the reaction system of GA-crosslinked β-glucosidase was analyzed by high performance liquid chromatography (HPLC).
研究领域:
生物技术
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酶学
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DOI
PubMed
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2016
·Vol. 40
·期 1
·1-7
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IF 1.901
Zhao Fuhua,Hou Tonggang,Wang Jianxun,Jiang Yijun,Huang Shuxia,Wang Qiao,Xian Mo,Mu Xindong
Enzyme immobilization is believed to provide an excellent base for increasing environmental tolerance of enzyme and considerable period of time. In this work, a kind of nonporous silica nanoparticles functionalized with amino group was synthesized to immobilize proline-specific endoprotease (PSEP). PSEP is known to specifically cleave peptides (or esters) at the carboxyl side of proline, thus can prevent the formation of haze and prolong the shelf life of beer. After immobilization, the environmental tolerance (temperature and pH, respectively) was obviously improved, and the immobilized enzyme can retain above 90 % of its original activity after 6 uses. Moreover, the immobilized enzyme can effectively prevent the formation of chill-haze using fresh beer fermentation liquid.
研究领域:
生物化学
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食品科学
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酶工程
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2016
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IF 7.145
Ming Li,Kang Lv,Shiqiang Wu,Shuiliang Chen
The anodophilic bacteria in the anodes of microbial fuel cells (MFCs) used to catalyze carbon oxidation are anaerobes and require anaerobic conditions; their bioelectrocatalytic activity will be greatly suppressed upon direct exposure to O2. In this study, an aerotolerant bioanode was fabricated for the first time by immobilization of anodophilic bacteria for use in MFCs operating under aerobic conditions. The fabrication of the aerotolerant bioanode was realized through the electrochemically induced penetration and propagation of anodophilic bacteria in a three-dimensional hydrogel scaffold. Under the protection of the hydrogel scaffold, the anodophilic bacteria exhibited excellent bioelectrocatalytic activity under continuous O2 aeration and delivered a current density comparable to that under anaerobic conditions. The MFC equipped with the aerotolerant bioanode has the potential to be applied to traditionally aerobic wastewater treatment (WWT) technology. This study offers new insight into the application of MFCs for WWT.
研究领域:
环境科学
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微生物学
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电化学
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DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
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2016
·Vol. 40
·期 4
·954-961
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IF 2.741
Yilin Li,Tao Bao,Zilin Chen
In this work, we developed a capillary column modified with zeolitic imidazolate framework-8 as a novel stationary phase for open-tubular capillary electrochromatography. To immobilize zeolitic imidazolate framework-8 onto the inner surface of silica capillary, a bio-inspired polydopamine functionalization was used to functionalize the capillary surface with polydopamine. First, a polydopamine layer was assembled inside the capillary. Second, due to noncovalent adsorption and covalent reaction ability, polydopamine could attract and anchor zeolitic imidazolate framework-8 onto the inner surface of capillary. It has been demonstrated that zeolitic imidazolate framework-8 was successfully grafted on the inner wall of the capillary by scanning electron microscopy, and Fourier transform infrared spectroscopy. The electro-osmotic flow characteristics of capillaries were also investigated by varying the pH value and acetonitrile content of mobile phase. The zeolitic imidazolate framework-8 coating not only increased the phase ratio of open-tubular column, but also improved the interactions between tested analytes and the stationary phase. Three groups of isomers including acidic, basic, and neutral compounds were well separated on the zeolitic imidazolate framework-8 bonded column, with theoretic plate numbers up to 1.9 × 10 5 N for catechol. The repeatability of the prepared columns was also studied, and the relative standard deviations for intra- and interday runs were less than 5%.
研究领域:
分析化学
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材料科学
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PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2017
·Vol. 409
·期 8
·2179-2187
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IF 3.431
Wang Heping,Jiao Fenglong,Gao Fangyuan,Zhao Xinyuan,Zhao Yan,Shen Yehua,Zhang Yangjun,Qian Xiaohong
Deep and efficient proteolysis is the critical premise in mass spectrometry-based bottom-up proteomics. It is difficult for traditional in-solution digestion to meet the requirement unless prolonged digestion time and enhanced enzyme dosage are employed, which makes the whole workflow time-consuming and costly. The abovementioned problems could be effectively ameliorated by anchoring many proteases on solid supports. In this work, covalent organic framework-coated magnetic graphene (MG@TpPa-1) was designed and prepared as a novel enzyme carrier for the covalent immobilization of trypsin with a high degree of loading (up to 268 μg mg −1 ). Profiting from the advantages of magnetic graphene and covalent organic frameworks, the novel trypsin bioreactor was successfully applied for the enzymatic digestion of a model protein with dramatically improved digestion efficiency, stability, and reusability. Complete digestion could be achieved in a time period as short as 2 min. For the digestion of proteins extracted from Amygdalus pedunculata , a total of 2833 protein groups were identified, which was slightly more than those obtained by 12 h of in-solution digestion (2739 protein groups). All of the results demonstrate that MG@TpPa-1-trypsin is an excellent candidate for sample preparation in a high-throughput proteomics analysis.
Covalent organic frameworks-coated magnetic graphene was prepared as novel carrier for highly efficient tryptic immobilization
研究领域:
生物化学
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蛋白质组学
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF BIOTECHNOLOGY
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2018
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IF 2.533
Binglin Li,Dandan Duan,Jiao Wang,Huanyu Li,Xiaoli Zhang,Binxia Zhao
Phospholipase D (PLD) was firstly hyperactivated by the bio-imprinting and, then, these hyperactivated PLD molecules were immobilized by adsorption and precipitation, followed by cross-linking. The high degree of conformational rigidification provided by intra- and intermolecular cross-linking can make PLD “remember” imprint-induced characteristics even in aqueous solutions. The obtained immobilized PLD showed the excellent catalytic performance. The maximum activity of immobilized PLD reached 166953 U/g protein , which was approximately 14 times higher than that of free form (11922 U/g protein ). Moreover, the selectivity of PLD was significantly enhanced after immobilization. The yield of PG and PA reached 94.0% and 5.96%, respectively. Compared with the serious hydrolysis in the free PLD (35.3% yield of PA), the side reaction was minimized in this work. This may be the first description of the remarkably high improvement of the activity and selectivity of PLD through the immobilization technology.
研究领域:
生物化学
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酶工程
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2019
·
IF 5.108
Enhui Wu,Yuexian Li,Qing Huang,Zhenkai Yang,Anyu Wei,Qi Hu
An amino-functionalized magnetic metal organic framework (MOF), Fe 3 O 4 -NH 2 @MIL-101(Cr), was employed for laccase immobilization for the first time. The immobilized laccase was synthesized by the adsorption and covalent binding method, thus exhibited high activity recovery, large immobilization capacity and good tolerance to low pH and high temperature conditions. The excellent stability enabled the immobilized laccase to retain 89% of its initial activity after storage for 28 days. When the ambient temperature reached 85 °C, the immobilized laccase showed 49.1% residual activity even after 6 h preservation. The stability of laccase in organic solvents such as methanol was also greatly improved. Application of the immobilized laccase for 2,4-dichlorophenol removal was also investigated. The adsorption by Fe 3 O 4 -NH 2 @MIL-101(Cr) contributed to a quick removal in the first hour, and the removal efficiency reached 87% eventually. When the reaction was completed, the immobilized laccase could be separated from the solution by a magnet. The results introduced a novel support for laccase immobilization, and the immobilized laccase had great potential in wastewater treatment .
研究领域:
环境科学
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生物技术
·
废水处理
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DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2019
·
IF 2.883
Raedah A.S. Alatawi,M. Monier,Nadia H. Elsayed
In this work, a photo-responsive hydrogel membrane based on cinnamate-modified hyaluronic acid (HA-CM) was developed and safely cross-linked under UV light curing. The obtained material was effectively utilized for immobilization of horseradish peroxidase (HRP) enzyme via encapsulation and entrapment strategy with efficiency above 95%. The prepared HA-CM biopolymer was investigated before the UV curing using instrumental and spectral techniques including Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR). During the UV irradiation, the progress of the cross-linking reaction was monitored by the UV–vis light spectroscopy. In addition, when the photo-induced cross-linking had accomplished, the morphological appearance of the hydrogel membrane was recorded using a scanning electron microscope (SEM). The HRP immobilized in HA-CM membranes displayed remarkable stability against the environmental pH changes especially under alkaline media and shift the optimum pH to 8 compared to the free HRP, which exhibited the highest activity at pH 7. Also, the entrapped enzyme was able to preserve above 85% of its catalytic activity at higher temperature values where the free enzyme had deactivated by approximately 50%. Moreover, HA-CM-HRP maintained 87% of its activity after 10 sequential reuse cycles, which indicate the economic value of the employed immobilization strategy.
研究领域:
生物材料
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酶工程
·
光化学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Na Chen,Yichao Wan,Guangming Zhan,Xiaobing Wang,Meiqi Li,Lizhi Zhang
Solar light driven organoarsenicals degradation and simultaneous inorganic arsenic immobilization with naturally abundant hematite is very promising for organoarsenicals remediation, but suffers from the fast electron-hole charge recombination of hematite. Herein, we report that oxalate, widely existed in soil and water environments, can greatly enhance the photocatalytic molecular oxygen activation of hematite to degrade a typical organoarsenical roxarsone and immobilize the resulted inorganic arsenic. It was found that oxalate could chelate with surface Fe(III) of hematite to generate plenty of •OH via a surface Fe(III)/Fe(II) cycle mediated oxygen molecular activation process under a simulated solar light, and thus induced 85.1% of roxarsone transformation into inorganic arsenic species, mainly As(V), within 6 h. The generated As(V) could be further adsorbed on hematite surface with prolonging the reaction time to 24 h in dark, avoiding secondary pollution. More importantly, hematite was reusable for the simulated solar light driven roxarsone degradation and As(V) immobilization in the presence of oxalate. This study offers a green and effective strategy for organoarsenicals remediation, and also sheds light on the environmental effects of iron oxides and oxalate.
研究领域:
环境科学
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光化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Yan Li,Jia-Qin Wu,Neng-Bing Long,Rui-Feng Zhang
In this article, novel epoxy resin-based hierarchical porous polymers (HPSs) have been prepared through a non-sol-gel and template-free approach using crystalline trimethylolpropane (TMP) as porogen. The polymers exhibit dimensional stability and possess 3-dimentional interconnected multi-scale pores. In range of 50 μm~10 nm are ultra-macro-pore in between skeleton, macro-pore on skeleton and meso-pore in network, respectively. The porosity and specific surface area can be adjusted in range of 91.2–82.5% and 225–156 m 2 /g, respectively. Using three kinds of hierarchical porous polymers as supports phospholipase D (PLD) was effectively immobilized through physical adsorption. Owing to high porosity of the support and improvement of mass transfer the loading amount of PLD reached as high as 223 mg/g support and the corresponding specific activity achieved up to 3.75 × 10 3 U/g support . Under optimized conditions and the phosphatidylserine (PS) yield reached 95.5% within 40 min at 45 °C. The immobilized PLD exhibited not only better storage stability and but also resistance to pH and thermal inactivation than free PLD. It was found that 73.5% of PS yield retained after 12 cycling reuses.
研究领域:
生物化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
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