ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Jun Liu,Yan Tang,Xiangyun Lin,Cheng Zhang,Nan Ma,Lei Wang,Wei Qu,Meiyu Shao,Yuxia Zhao,Wei Zhang
Thrombus formation associated with blood-contacting catheters remains a prevalent clinical issue. Antithrombotic coatings capable of inhibiting coagulation activation without interfering with hemostasis are highly desired. Herein, we constructed thick and densely packed poly(ε-acryloyl l-lysine) (p(LysAA)) zwitterionic brushes on the inner wall of chemical inert polyethylene (PE) catheters via a recently developed universal, substrate-independent coating strategy that enables facile scaling-up for catheters with complex geometries. The densely packed l-lysine-derived zwitterionic brushes exhibited ultrahydrophilic properties, with water contact angles below 10°. As expected, fouling by bovine serum albumin, fibrinogen, bacteria, platelets, and red blood cells was effectively suppressed. Moreover, the brushes demonstrated excellent biocompatibility, as evidenced by relative cell growth rates of over 90%, a hemolysis rate of below 1%, and intradermal reaction scores of less than 1.0. Importantly, it showed antithrombotic efficacy both in vitro and an in vivo. In a rabbit arterio-jugular vein shunt model, no obvious clotting was observed after 1 h of circulation. Overall, the brushes hold significant clinical potential in reducing the level of thrombus formation for blood-contacting catheters. Furthermore, by leveraging the abundant reactive amine and carboxyl groups within the p(LysAA) brushes, this approach provides a platform for integrating bioactive molecules to further mitigate the thrombotic risk.
研究领域:
生物医学工程
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生物材料
·
表面科学
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抗凝治疗
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医疗器械
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产品关联
DOI
PubMed
高影响因子
Advanced Materials Interfaces
·
2022
·Vol. 9
·期 16
·2102590
·
IF 6.389
Bin Zhou,Guohua Li,Mingxi Wu,Zhangkai Zhou,Xin Cai,Jingxuan Cai,Jianhua Zhou
A platform for real-time monitoring and comprehensively studying the adhesion process of microorganisms onto material surfaces as well as their bioactivity on the surface is very helpful to reveal the microbial contamination mechanism on drainage catheters, intravascular stents, and implanted sensors. Herein, a multifunctional sensor based on localized surface plasmon resonance of gold nano-match head arrays (GNMAs) is developed for monitoring the adhesion behavior of microorganisms (such as Candida albicans , i.e., C. albicans ) as well as its inhibitory activity. The sensor is fabricated through soft lithography processes using nanopatterned polydimethylsiloxane stamps, and modified with polymers of different charges for investigating the adhesion of C. albicans onto different charged surfaces. The spectroscopic study shows that C. albicans are more favorable to positively charged surfaces than negatively charged ones, which is also proven by the fluorescence images under microscope. Moreover, the authors demonstrate that the GNMA modified with poly- l -lysine (PLL) can effectively kill the adhered C. albicans due to the membrane destruction and the reactive oxygen species induced by PLL. These unique advantages suggest that the sensor is capable as both an antifungal study system and a real-time monitoring platform for antifungal performance evaluation.
研究领域:
生物医学工程
·
微生物学
·
纳米技术
·
材料科学
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DOI
高影响因子
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
·
2018
·Vol. 96
·期 1
·15-23
·
IF 1.601
Saisai Li,Yan Li,Nengbing Long,Ruifeng Zhang
Phospholipase D (PLD) was effectively immobilized on a nanocomposite support composed of ZnO nanowires and SiO 2 through in-situ co-cross-linking with poly-L-lysine (PLL). An anionic and long-chained bisepoxide cross-linker was electrostatically adsorbed on the surface of ZnO nanowires prior to co-cross-linking. Under the optimal pH, 6.5, the loading amount of PLD reached an average of 114 mg per g support for PLL concentrations in the range of 0–0.6 mg mL −1 . Co-cross-linking of PLD with PLL enhanced the activity of PLD remarkably. The highest specific activity of co-cross-linked PLD reached 25.8 U per mg protein , which was 1.62 times as high as that of the cross-linked PLD (15.9 U per mg protein ). (One unit of PLD activity [U] refers to the amount of PLD, which liberates 1 μmol of p-nitrophenol per min via hydrolysis of phosphatidyl-p-nitrophenol at pH 8.0 and 30 °C). Under the optimized conditions, the yield of phosphatidylglycerol (PtdGro) reached 98.6% within 80 min at 45 °C and pH 6.5. The co-cross-linked PLD not only exhibited good resistance to pH and heat inactivation but also improved storage stability and reusability. The yield of PtdGro remained ≥85.4% after 13 repeat uses.
研究领域:
生物化学
·
酶工程
·
材料科学
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DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2016
·Vol. 163
·期 7
·B272
·
IF 3.014
Hao Gou,Jingxian He,Zunli Mo,Xiaojiao Wei,Rere Hu,Yawen Wang,Ruibin Guo
A fast electrochemical chiral sensor based on L-lysine (L-Lys) covalent functionalize reduced graphene oxide (RGO) has been developed for electrochemical recognize of tryptophan (Trp) enantiomers. The reduced graphene oxide/L-Lys (RGO/L-Lys) chiral composites with excellent water-soluble and biocompatible is very suitable for electrochemical sensor. Meanwhile, the L-Lys retains original chirality in RGO/L-Lys composites, which progress to electrochemical chiral sensor. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) were implemented to monitor the electrochemical behavior when RGO/L-Lys simply modified on glassy carbon electrode (RGO/L-Lys/GCE). Surprisingly, the reduction of peak current was significantly different after the RGO/L-Lys composites interacted with L-Trp or D-Trp, and the testing time is very short. It suggested that the RGO/L-Lys/GCE can be used as an electrochemical chiral sensor for the discrimination of Trp enantiomers. In this electrochemical discrimination process, the three-point interaction between L-Lys and Trp enantiomers has played an important role. Further research indicated that the decreased of peak currents versus percentage of L-Trp of Trp racemic mixture exhibits a fine linear relationship, it provides opportunity to make analysis for discrimination of Trp enantiomers. The RGO/L-Lys/GCE electrochemical chiral sensor with rapid recognition, good sensitivity and high stability provided an efficient method to recognize and determine Trp enantiomers.
研究领域:
分析化学
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生物化学
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DOI
高影响因子
Green Energy & Environment
·
2020
·
IF 6.395
Jiake Wang,Yan Wang,Zhongzheng Ma,Lifeng Yan
Green solvents for cellulose dissolution is a key topic for green chemistry, especially natural cellulose with high molecular weight, and there are scarce solvents reported. Deep eutectic solvent (DES) is a typical kind of green solvent that has been attracted much attention recently. Here, high molecular weight natural cellulose (DP > 3000) was first isolated from wheat straw and then be directly dissolved in the choline/ l -lysine (Ch/Lys) DES. The solution owns excellent stability, and the solubility reaches ∼5%. Rheological studies revealed that the natural cellulose can be well dispersed in the DES solution and showed gelation at high concentrations. The dissolved cellulose can be regenerated when the dilute acid aqueous solution was added into the solution. It provides an energy conversation and an environmentally friendly route to prepare a cellulose solution, which makes it possible to convert cellulose to valuable chemicals and materials in its homogeneous solution.
研究领域:
化学工程
·
材料科学
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DOI
高影响因子
Journal of Energy Storage
·
2026
·
IF 10.7
Chuang Wang,Lingzhuo Yang,Jinxiang Long,Tao Xie,Chuang Li,Yanhong Xiang,Xianming Wu,Xianwen Wu
With the growing demand for sustainable energy, aqueous zinc-ion batteries (AZIBs) have attracted considerable attention as an emerging energy-storage technology owing to their low cost and high safety. However, one of the most widely used cathode materials of manganese dioxide (MnO 2 ) for AZIBs suffers from unstable electrochemical performance of sluggish zinc-ion diffusion and gradual structural degradation. To solve these problems, we propose a simple in-situ pre-intercalation strategy to construct lysine-functionalized δ-MnO 2 (Lys-MnO 2 ), thereby reinforcing the structural stability and boosting the electrochemical performance of MnO 2 . XRD and HRTEM characterizations, combined with GITT and EIS analyses, demonstrate that lysine molecules effectively increase the interlayer spacing of MnO 2 and facilitate zinc-ion diffusion. Electrochemical tests show that the Lys-MnO 2 cathode exhibits the markedly enhanced cycling performance. It maintains a reversible capacity of over 274 mAh g −1 after 100 cycles at 0.2 A g −1 and retains 92.7% of its capacity even after 900 cycles at 1 A g −1 , superior to those of the pristine MnO 2 cathode. This work provides an innovative route for the development of high-performance and long-lifespan manganese-based cathode materials for AZIBs.
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DOI
高影响因子
Acta Biomaterialia
·
2016
·
IF 6.008
Weijun Xu,Junmin Qian,Yaping Zhang,Aili Suo,Ning Cui,Jinlei Wang,Yu Yao,Hejing Wang
To mimic the structure of breast tumor microenvironment, novel double-network poly(N ɛ -acryloyl l -lysine)/hyaluronic acid (pLysAAm/HA) hydrogels were fabricated by a two-step photo-polymerization process for in vitro three-dimensional (3D) cell culture. The morphology, mechanical properties , swelling and degradation behaviors of pLysAAm/HA hydrogels were investigated. The growth behavior and function of MCF-7 cells cultured on the hydrogels and standard 2D culture plates were compared. The results showed that pLysAAm/HA hydrogels had a highly porous microstructure with a double network and that their mechanical properties, swelling ratio and degradation rate depended on the degree of methacrylation of HA. The results of in vitro studies revealed that the pLysAAm/HA hydrogels could support MCF-7 cell adhesion , promote cell proliferation , and induce the diversification of cell morphologies and overexpression of VEGF, IL-8 and bFGF . The MCF-7 cells cultured on 3D hydrogels showed significantly increased migration and invasion abilities as compared to 2D-cultured cells. Preliminary in vivo results confirmed that the 3D culture of MCF-7 cells resulted in greater tumorigenesis than their 2D culture. These results indicate that the pLysAAm/HA hydrogels can provide a 3D microenvironment for MCF-7 cells that is more representative of the in vivo breast cancer. Statement of Significance Traditional 2D cell cultures cannot ideally represent their in vivo physiological conditions. In this work, we reported a method for preparing double-network poly(N ɛ -acryloyl l -lysine)/hyaluronic acid hydrogel, and demonstrated its suitability for use in mimicing breast tumor microenvironment. Results showed the prepared hydrogels had controllable mechanical properties, swelling ratio and degradation rate. The MCF-7 cells cultured in hydrogels expressed much higher levels of pro-angiogenic growth factors and displayed significantly enhanced migration and invasion abilities. The tumorigenic capability of MCF-7 cells pre-cultured in 3D hydrogels was enhanced significantly. Therefore, the novel hydrogel may provide a more physiologically relevant 3D in vitro model for breast cancer research. To our knowledge, this is the first report assessing a HA-based double-network hydrogel used as a tumor model.
研究领域:
生物材料
·
组织工程
·
癌症研究
·
细胞生物学
·
生物医学工程
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Yaxuan Zhao,Xuan Zhang,Min Sun,Ni An,Yuhuan Chen,Fang Yang
The poor water solubility of anticancer drugs significantly limits their therapeutic efficacy. Amphiphilic micelles with hydrophilic shells have emerged as promising anticancer drug carriers due to their remarkable ability to improve drug bioavailability. In this study, a Lysine-Arginine polyesteramide containing pendant carboxyl groups was synthesized (Lys-Arg-PEA). Subsequently, a series of amphiphilic comb-like copolymer micelle (AcDex-Lys-Arg-PEA) was developed through combination with acetylated dextran derivatives of different substitution degrees. The results revealed that the blank micelles maintained stable spherical structures with a uniform size distribution. The micellar system exhibited favorable hemocompatibility (hemolysis rate <5% and protein adsorption <30%), negligible cytotoxicity (cell viability >90% at 80 μg/mL concentration) and enzymatic degradability. Curcumin (Cur) was employed as a model drug to evaluate the anticancer drug delivery potential of this system. The micelles achieved high encapsulation efficiency (63%–85%) and drug loading capacity. Cur-loaded micelles showed significant inhibitory effects on HeLa cells (cell viability <55% at 80 μg/mL concentration). Drug release simulations in PBS buffer revealed pH-responsive characteristics, and the micelles exhibited faster Cur release at a cancerous pH (5.4, 50%–60%) than at a physiological pH (7.4, 30%–40%). This study demonstrates the potential of the comb-like copolymer micelles as effective carriers for anticancer drug delivery, providing valuable insights for subsequent research.
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DOI
PubMed
高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2022
·Vol. 31
·期 1
·131-148
·
IF 4.705
Wang Yanli,He Junwei,Sun Hong,Zou Liming,Li Yan Vivian
Hyperbranched poly (phenylalanine-lysine) (HBPL) is obtained by phenylalanine and lysine and then mixed with Polyvinyl Alcohol (PVA) polymer to electrospin PVA/HBPL fluorescent nanofiber mats (FNM), which is an excellent sensing material for Cu 2+ ions in aqueous solutions. In this work, the relationship between mechanical properties, fluorescent properties and the sensing for Cu 2+ ions of PVA/HBPL FNM are investigated. PVA/HBPL FNM with different mass ratio of PVA and HBPL are characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectra, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), fluorescence spectrum and tensile tester. To further improve the water solubility of PVA/HBPL FNM, thermally cross-linking (TCL)-PVA/HBPL FNM is fabricated. The results indicate that the neat PVA FNM couldn’t emit fluorescence under any excitation wavelength, whereas the PVA/HBPL FNM emit blue fluorescence green fluorescence when the irradiation of the excitation wavelength is at 330–385 nm and 450–480 nm, respectively. The tensile strength and Young’s modulus of PVA/HBPL FNM are 8.67 MPa and 342.92 MPa, respectively, which suggested 43.1% and 242.7% than that of neat PVA NM. Furthermore, the solubility rate of TCL-PVA/HBPL FNM is significantly decreased in comparison of FNM in absent of cross-linking. It’s worth noting that TCL-PVA/HBPL FNM possessed higher recognition than other mental ions and could be used as metal ion detection sensor to combine with Cu 2+ . Moreover, it represented a linear relationship between the fluorescence intensity and the concentration of Cu 2+ in the range of 5 µM to 100 µM. The fabricated TCL-PVA/HBPL FNM exhibit high mechanical and fluorescence properties, which make it be potentially envisaged for heavy metal ions detection sensor.
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DOI
高影响因子
ACS Omega
·
2021
·
IF 3.512
Zili Song,Ruilin Zhao,Hongjiao Zhang,Penglin Wei,Landa Qi,Guocan Chen,Wen-Bing Yin,Wei Li
Edible mushrooms are important nutraceutical sources of foods and drugs, which can produce various nutritional ingredients including all essential amino acids. The method of rapid screening for the strains producing specific functional components is very indispensable. Homocitrate synthase is one of the key enzymes in the α-aminoadipate pathway for lysine biosynthesis and has preferable sequence conservation in Agaricales. Based on the blast of homocitrate synthase homologous genes of strains of Agaricales, we achieved combinations of degenerate primers as molecular markers to rapidly screen the lysine-producing edible mushrooms. The experimental results revealed that the consistency between PCR amplification and HPLC analysis attained 82 and 75% in strains of Agaricales and Polyporales, respectively. The finding showed that the molecular marker has higher universality for screening edible mushroom resources of Agaricales. This PCR-based approach shows excellent potential in evaluating and discriminating edible wild-grown mushrooms with high lysine content in Agaricales.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2020
·
IF 7.727
Zhihao Guo,Junhui Sui,Mengcheng Ma,Jianshe Hu,Yong Sun,Liqun Yang,Yujiang Fan,Xingdong Zhang
Stimuli-responsive nanotechnology-mediated drug co-delivery system is a notable strategy to improve access of the systemically administered chemotherapeutics to the tumors. Herein, a tailor-made 2,3-dimethylmaleic-anhydride-poly(ethylene glycol)- ε -poly- l -lysine-doxorubicin /lapatinib polymeric nanoplatform (DMMA-P-DOX/LAP) for synergistically eliminating breast cancer is developed by encapsulating lapatinib into dual-pH responsive charge switchable biopolymer-doxorubicin conjugate nanoparticles . The physicochemical properties of polymeric nanoparticles are conducive to their stable circulation in the physiological condition, but reverse the surface charge from negative to positive ultrasensitively in slightly acidic tumor microenvironment , facilitating cell internalization and deep tumor penetration. Subsequently, DOX and LAP are synchronously released into the cytoplasm in response to the significantly increased acidity of intracellular environment. As a result, the combination therapy by DMMA-P-DOX/LAP nanoparticles compels the solid tumors to contract significantly or even vanish completely in the MCF-7 tumor model, moreover, the structural composition with amino acid and bioinert PEG ensures the favorable biosecurity of the co-delivery system in vivo . This dual-pH responsive nanotechnology-mediated drug co-delivery system provides great potentials for safe and effective cancer therapy.
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2019
·
IF 4.527
Yang Wu,Yan Li,Liangqing Zheng,Pengcheng Wang,Yan liu,Yongning Wu,ZhiYong Gong
Nε-(carboxymethyl)lysine (CML) is a potentially noxious compound that is causing widespread concern due to its use in various food products. In this study, we investigated CML neurotoxicity via an in vivo experiment with mice, and an in vitro experiment using a 3D microvascular network model (with human brain vascular endothelial cell and human astrocyte) that simulated the blood-brain barrier. We found that CML could induce cell survival status variations, and histopathological changes to the brain. In addition, CML increased levels of oxidative stress , prompted the protein expression of the receptor for advanced glycation end-products (RAGE). CML up-regulated both the gene expression of RAGE, the activating protein-1 (AP-1), the inflammatory cytokines Interleukin-6 (IL-6), vascular cell adhesion molecule1 (VCAM-1), monocyte chemotactic protein1 (MCP-1). We, therefore, postulated that CML has the potential to deleteriously affect the nervous system through oxidative stress and that activation of the p38 MAPK-AP-1 signaling pathway might be implicated in this pathological process.
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DOI
PubMed
高影响因子
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