JOURNAL OF NANOBIOTECHNOLOGY
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2024
·
IF 10.6
Chengjun Song,Jiamin Wu,Jinhui Wu,Fangyu Wang
Background Ulcerative colitis (UC) is one chronic and relapsing inflammatory bowel disease. Macrophage has been reputed as one trigger for UC. Recently, phosphodiesterase 4 (PDE4) inhibitors, for instance roflumilast, have been regarded as one latent approach to modulating macrophage in UC treatment. Roflumilast can decelerate cyclic adenosine monophosphate (cAMP) degradation, which impedes TNF-α synthesis in macrophage. However, roflumilast is devoid of macrophage-target and consequently causes some unavoidable adverse reactions, which restrict the utilization in UC. Results Membrane vesicles (MVs) from probiotic Escherichia coli Nissle 1917 (EcN 1917) served as a drug delivery platform for targeting macrophage. As model drugs, roflumilast and MnO 2 were encapsulated in MVs (Rof&MnO 2 @MVs). Roflumilast inhibited cAMP degradation via PDE4 deactivation and MnO 2 boosted cAMP generation by activating adenylate cyclase (AC). Compared with roflumilast, co-delivery of roflumilast and MnO 2 apparently produced more cAMP and less TNF-α in macrophage. Besides, Rof&MnO 2 @MVs could ameliorate colitis in mouse model and regulate gut microbe such as mitigating pathogenic Escherichia–Shigella and elevating probiotic Akkermansia . Conclusions A probiotic-based nanoparticle was prepared for precise codelivery of roflumilast and MnO 2 into macrophage. This biomimetic nanoparticle could synergistically modulate cAMP in macrophage and ameliorate experimental colitis. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1186/s12951-024-02558-6.
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Scientific Reports
·
2024
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IF 3.8
Si-Chun Gu,Zhi-Guo Xie,Min-Jue Gu,Chang-De Wang,Li-Min Xu,Chen Gao,Xiao-Lei Yuan,You Wu,Yu-Qing Hu,Yang Cao,Qing Ye
Ferroptosis is an iron-dependent cell death form characterized by reactive oxygen species (ROS) overgeneration and lipid peroxidation. Myricetin, a flavonoid that exists in numerous plants, exhibits potent antioxidant capacity. Given that iron accumulation and ROS-provoked dopaminergic neuron death are the two main pathological hallmarks of Parkinson’s disease (PD), we aimed to investigate whether myricetin decreases neuronal death through suppressing ferroptosis. The PD models were established by intraperitoneally injecting 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) into rats and by treating SH-SY5Y cells with 1-methyl-4-phenylpyridinium (MPP + ), respectively. Ferroptosis was identified by assessing the levels of Fe 2+ , ROS, malondialdehyde (MDA), and glutathione (GSH). The results demonstrated that myricetin treatment effectively mitigated MPTP-triggered motor impairment, dopamine neuronal death, and α-synuclein (α-Syn) accumulation in PD models. Myricetin also alleviated MPTP-induced ferroptosis, as evidenced by decreased levels of Fe 2+ , ROS, and MDA and increased levels of GSH in the substantia nigra (SN) and serum in PD models. All these changes were reversed by erastin, a ferroptosis activator. In vitro, myricetin treatment restored SH-SY5Y cell viability and alleviated MPP + -induced SH-SY5Y cell ferroptosis. Mechanistically, myricetin accelerated nuclear translocation of nuclear factor E2-related factor 2 (Nrf2) and subsequent glutathione peroxidase 4 (Gpx4) expression in MPP + -treated SH-SY5Y cells, two critical inhibitors of ferroptosis. Collectively, these data demonstrate that myricetin may be a potential agent for decreasing dopaminergic neuron death by inhibiting ferroptosis in PD.
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Science China-Chemistry
·
2025
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IF 9.7
Wu Min,Nan Mingjun,Liu Shumin,Zhong Caihe,Qiao Lin,Zhang Huamin,Ma Xiangkun
With the increasing demand for energy storage technology, iron-chromium flow batteries (ICFBs) have been widely concerned because of their price advantage. However, the low electrochemical activity of Cr 3+ /Cr 2+ redox couples and the side hydrogen evolution reaction limit the industrial application of ICFBs. A multi-dimensional Bi/carbon composite electrocatalyst (Bi@C) for ICFB is designed and prepared to improve the electrochemical activity of Cr 3+ /Cr 2+ redox couples. Benefiting by using the Bimetal organic framework (Bi-MOF) with solid matrix as precursors, Bi nanospheres are highly dispersed on the Bi@C electrocatalyst that effectively enhances the electrochemical activity. The special morphology of Bi@C electrocatalyst helps the transfer of electrons and ions, significantly reducing the polarization of battery. Herein, the 3D porous carbon frames accelerate mass transfer, and the 2D carbon nanobelts and carbon layer coating on 0D Bi nanospheres improve the conductivity of Bi nanospheres. Therefore, the ICFB with multi-dimensional Bi@C electrocatalyst exhibits coulombic efficiency of 98.10% and energy efficiency of 79.14% at 140 mA cm −2 , which is higher than ICFBs with commercial graphite carbon electrocatalyst and with heat treatment carbon felt. This work provides a simple and economical method to fabricate a high-performance multidimensional Bi@C electrocatalyst for Cr 3+ /Cr 2+ redox couples, boosting the development of ICFBs.
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Nature Nanotechnology
·
2025
·
IF 34.9
Dong Jianchao,Zhao Bin,Ji Huiyu,Zang Ziang,Kong Lingmei,Chu Chunshuang,Han Dongyuan,Wang Jie,Fu Yuhao,Zhang Zi-Hui,Yang Yingguo,Zhang Lijun,Yang Xuyong,Wang Ning
Despite substantial advances in green and red metal halide perovskite light-emitting diodes (PeLEDs), blue PeLEDs, particularly deep-blue ones (defined as Commission International de l’Eclairage y coordinate (CIE y ) less than 0.06) that meet the latest Rec. 2020 colour gamut standard, lag dramatically behind owing to a severe phase segregation-induced electroluminescent spectral shift and low exciton utilization in broadened bandgap perovskite emitters. Here we propose a multivalent immobilization strategy to realize high-efficiency and spectrally stable deep-blue PeLEDs by introducing a polyfluorinated oxygen-containing molecule. Systematic experiments and extensive 5,000 fs ab initio molecular dynamics simulations reveal that a crucial role of the multivalent effect stemming from three kinds of interaction of hydrogen bond (F···H–N), ionic bond (F–Pb) and coordination bond (C=O:Pb) with perovskite is to synergistically stabilize the perovskite phase and enhance exciton radiative recombination. The resultant exciton concentration and exciton recombination rate of the deep-blue perovskite emitter are increased by factors of 1.66 and 1.64, respectively. In this context, our target PeLEDs demonstrate a peak external quantum efficiency of up to 15.36% at a deep-blue emission wavelength of 459 nm and a half-lifetime of 144 min at a constant current density of 0.45 mA cm − 2 . Moreover, the deep-blue PeLEDs maintain a constant spectrum peak with CIE chromaticity coordinates of (0.136, 0.051) under a steady driving current for 60 min.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-14
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IF 5.3
Ren Shufang,Liu Xiaohang,Liu Yahui,Zhao Junpeng,Zhang Yuan,Zheng Zhixiang
A novel voltammetric sensor designed for dopamine (DA) detection is presented utilizing a combination of zeolitic imidazolate framework (ZIF-67) derived cobalt and nitrogen-doped carbon on reduced graphene oxide (Co–N-C/rGO). ZIF-67 cubic crystals were synthesized in situ and deposited onto the graphene oxide (GO) surface through room-temperature reactions. High-temperature calcination resulted in partially collapsed cubic and spherical carbon, while simultaneously reducing GO to rGO. A molecular imprinting resorcinol polymer (MIP) membrane was also in situ applied to the Co–N-C/rGO/glassy carbon electrode (GCE) via electropolymerization. Analyses using cyclic voltammetry, electrochemical impedance, and pulse voltammetry reveal that the modified MIP/Co–N-C/rGO/GCE electrodes show improved electroconductivity and notable electrochemical reactivity towards dopamine. After optimizing detection parameters, the sensor demonstrates a wide linear detection range of 0.01—0.5 and 0.5—100 μmol/L, with a limit of detection (LOD) of 3.33 nmol/L (S/N = 3). Additionally, the sensor displays strong robustness, including excellent selectivity, significant resistance to interference, and long-term stability. It also shows satisfactory recovery in detecting spiked real samples. Graphical
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MOLECULAR BIOLOGY REPORTS
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2025
·Vol. 52
·期 1
·1-13
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IF 2.8
Wu Wanqi,Wang Jun,Wang Guanglu,Wang Feibiao,Yang Yue,Liu Zhijun,Song Qimei,Chen Si,Chen Huizhen
Background Sepsis can cause severe cardiac damage, and matrix metalloproteinase 9 (MMP9) is involved in the inflammatory response and tissue injury processes. Monotropein is a monoterpene glycoside with anti-inflammatory and antioxidant effects. This study aims to investigate whether monotropein can alleviate sepsis-induced cardiac injury by affecting the activity of MMP9. Methods and results The correlation between MMP9 and septic cardiac injury was explored using differential expression gene analysis from the GEO database and an in vitro lipopolysaccharide (LPS)-stimulated H9c2 cell model. In a cecal ligation and puncture (CLP)-induced mouse sepsis model, the effects of monotropein on myocardial cell apoptosis, inflammatory factor expression, and antioxidant enzyme levels were validated through drug administration. The results showed that MMP9 was significantly upregulated in sepsis patients. In the H9c2 cell model, LPS-induced MMP9 activity was positively correlated with cell damage. Inhibition of MMP9 alleviates LPS-induced myocardial matrix disruption and apoptosis. Monotropein exerts anti-matrix degradation and anti-apoptotic effects through MMP9 in LPS-induced H9c2 cells. Monotropein also reduced the expression of LPS-induced inflammatory factors (TNF-α, IL-1β, IL-6).In the mouse model, monotropein decreased oxidative stress damage (lower MDA levels, increased GSH, T-AOC, CAT enzyme activity), and improved cardiac injury by inhibiting myocardial cell apoptosis-related proteins (Bax, Bcl-2, and Caspase-3 activation). Conclusion Monotropein exerts a protective effect on septic cardiac injury by inhibiting MMP9 activity, reducing inflammatory response, and enhancing antioxidant capacity, thereby ameliorating myocardial cell damage and apoptosis. Graphical abstract
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JOURNAL OF MATERIALS SCIENCE
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2024
·Vol. 59
·期 27
·12347-12360
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IF 3.5
Ding Xiuping,Tao Jianghui,Huang Liangzhu,Wang Yabin,Wang Yanni
Dendritic mesoporous silica nanospheres (DMSNs) possess excellent specific surface areas, pore volumes, and extremely accessible internal spaces. DMSNs have experienced high-speed development in the aspects of synthesis techniques, functionalization strategies, and application fields. It is proved that DMSNs own inherent structural superiorities as catalysts, adsorbents, or reaction platforms. Naturally, researchers are enlightened to conceive and attempt to synthesize dendritic mesoporous titania nanospheres (DMTNs) by replacing Si in DMSNs with Ti, in view of the superior activity and catalytic performance from TiO 2 . Nevertheless, the hydrolysis and condensation rates of the titanium precursors are too fast to develop ideal dendritic textures. To get the goal in a roundabout way, hybrid dendritic mesoporous silica&titania nanospheres (DMSTNs) come into sight. In this work, a series of DMSTNs have been synthesized by the one-pot co-condensation method. For the first time, their morphologies and architectures have been controlled by adjusting the ratio of titanium to silica, stirring speed, reaction temperature, co-solvent, etc. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) have been utilized to directly reveal their differences. The basic physicochemical properties of DMSTNs with fine topological structures have been compared, covering Fourier Transform Infrared spectroscopy (FT-IR), X-ray diffraction (XRD), N 2 adsorption–desorption isotherms, Raman spectrum, X-ray photoelectron spectroscopy (XPS), ultraviolet–visible diffuse reflectance spectroscopy (UV-Vis-DRS), photoluminescence (PL) spectra, etc. Most importantly, these typical DMSTNs can photo-catalytically produce more hydrogen (2.4 ~ 3.6 times) within 1% Pt than that of bare DMSNs under simulated sunlight, owing to Ti in their skeletons. Graphical Abstract
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CATALYSIS LETTERS
·
2024
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IF 2.3
Feng Guo,Wang Xu,Wang Yajie,Sun Hongxin,Li Guohua
In PMS catalytic oxidation studies, we design and prepare a Mn 3 O 4 /CuO (CMO-2) composite catalyst using metal–organic frameworks (MOFs) as precursors. Compared with Mn 3 O 4 , its activated per-oxy-monosulfate activation catalyzed the degradation of pollutants with nearly six times higher activity efficiency. The experimental findings demonstrate that CMO-2 derived from metal–organic frameworks offer the advantages of wide adaptability to solution pH, along with improved versatility, stability, and recyclability. Further experiments involving radical scavenging, electrochemistry, and electron spin resonance (EPR) techniques confirm that the CMO-2 derived from MOF structures exhibit more significant interfacial engineering effects. This leads to an expansion of active interfaces, enhancing the accessibility and dispersion of active sites. Consequently, it significantly improves the efficiency of electron-transfer reactions and promotes a wide range of reactive oxygen species (ROSs), including SO 4 •− •OH and 1 O 2 . Graphical Abstract Based on the synergistic effect of copper-manganese bimetallic, the copper-manganese composite catalyst (CMO-2) prepared by doping 0.5 g of Cu-MOF showed the best catalytic activity in activating PMS, and the resulting ROS S could efficiently degrade organic pollutants.
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Journal of Translational Medicine
·
2024
·Vol. 22
·期 1
·1-12
·
IF 6.1
Tan Yue-Rong,Shen Si-Yang,Li Xin-Yi,Yi Peng-Fei,Fu Ben-Dong,Peng Lu-Yuan
Ulcerative colitis (UC) is an idiopathic, chronic inflammatory condition of the colon, characterized by repeated attacks, a lack of effective treatment options, and significant physical and mental health complications for patients. The endoplasmic reticulum (ER) is a vital intracellular organelle in maintaining cellular homeostasis. Endoplasmic reticulum stress (ERS) is induced when the body is exposed to adverse external stimuli. Numerous studies have shown that ERS-induced apoptosis plays a vital role in the pathogenesis of UC. Mogroside V (MV), an active ingredient of Monk fruit, has demonstrated excellent anti-inflammatory and antioxidant effects. In this study, we investigated the therapeutic effects of MV on dextran sulfate sodium (DSS)-induced UC and its potential mechanisms based on ERS. The results showed that MV exerted a protective effect against DSS-induced UC in mice as reflected by reduced DAI scores, increased colon length, reduced histological scores of the colon, and levels of pro-inflammatory cytokines, as well as decreased intestinal permeability. In addition, the expression of ERS pathway including BIP, PERK, eIF2α, ATF4, CHOP, as well as the apoptosis-related protein including Caspase-12, Bcl-2 and Bax, was found to be elevated in UC. However, MV treatment significantly inhibited the UC and reversed the expression of inflammation signaling pathway including ERS and ERS-induced apoptosis. Additionally, the addition of tunicamycin (Tm), an ERS activator, significantly weakened the therapeutic effect of MV on UC in mice. These findings suggest that MV may be a therapeutic agent for the treatment of DSS-induced UC by inhibiting the activation of the ERS-apoptosis pathway, and may provide a novel avenue for the treatment of UC.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-14
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IF 15.7
Zhou Laicheng,Zhu Longchen,Wang Cong,Xu Tengyan,Wang Jing,Zhang Bin,Zhang Xin,Wang Huaimin
Biomolecular condensates, formed by liquid-liquid phase separation of biomacromolecules, play crucial roles in regulating physiological events in biological systems. While multiphasic condensates have been extensively studied, those derived from a single component of short peptides have not yet been reported. Here, we report the symmetrical core-shell structural biomolecular condensates formed with a programmable tetrapeptide library via phase separation. Our findings reveal that tryptophan is essential for core-shell structure formation due to its strongest homotypical π-π interaction, enabling us to modulate the structure of condensates from core-shell to homogeneous by altering the amino acid composition. Molecular dynamics simulation combined with cryogenic focused ion beam scanning electron microscopy and cryogenic electron microscopy show that the inner core of multiphasic tetrapeptide condensates is solid-like, consisting of ordered structures. The core is enveloped by a liquid-like shell, stabilizing the core structure. Furthermore, we demonstrate control over multiphasic condensate formation through intrinsic redox reactions or post-translational modifications, facilitating the rational design of synthetic multiphasic condensates for various applications on demand.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-10
·
IF 5.3
An Jia,Yuan Mengdi,Han Yaqin,Liu Yufei
The rampant hepatitis B virus (HBV) seriously endangers human health, and hepatitis B surface antigen (HBsAg) is its early diagnostic marker. Therefore, it is crucial to construct a fast and highly sensitive HBsAg detection method. Based on high-efficiency magnetic separation technology and fluorescent composite material labelling technology, an accurate, fast and sensitive fluorescent immunosensing system for HBsAg detection was developed. Immunomagnetic beads constructed from carboxyl-functionalized Fe 3 O 4 nanoparticles (Fe 3 O 4 -COOH) with excellent magnetic response performance were used as efficient capture carriers for HBsAg. Immunofluorescence composite microspheres constructed based on ultra-stable polystyrene-coated CsPbBr 3 perovskite nanocrystals (CPB@PSAA) with high hydrophilic properties, were excellent fluorescent markers for HBsAg. Using this sensitive sandwich fluorescence sensing system a good linear relationship within the range of 0.2–15 ng/mL was established between HBsAg concentration and fluorescence intensity with a limit of detection (LOD) of 0.05 ng/mL. The system obtained satisfactory results when tested on real human serum samples. The magnetic-assisted fluorescence immune-sandwich sensor system has broad application prospects in biomedicine such as rapid and early diagnosis and effective prevention of infectious diseases. Graphical Abstract
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Friction
·
2024
·Vol. 12
·期 7
·1564-1579
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IF 6.3
Duan Linlin,Jia Dan,Li Jian,Liu Jianfang,Duan Haitao
In this work, the advantage of Coulomb repulsion in the intermolecular forces experienced by molecules on the solid-liquid nanosized contact interface is taken, and the superior friction-reducing property of Cu 3 (PO 4 ) 2 ·3H 2 O (CuP) oil-based additives has been confirmed for titanium alloy. Three-dimensional (3D) CuP nanoflowers (CuP-Fs) with a strong capillary absorption effect are prepared to achieve the homogeneous mixing of solid CuP and lubricating oil. Lubrication by CuP-Fs additives for titanium alloy, friction coefficient (COF) can be reduced by 73.68%, and wear rate (WR) reduced by 99.69%. It is demonstrated that the extraordinary friction-reducing property is due to the repulsive solid-liquid interface with low viscous shear force originating from Coulomb repulsion between polar water molecules in CuP and non-polar oil molecules. However, any steric hindrance or connection between this repulsive solid-liquid interface will trigger the adhesion and increase the viscous shear force, for example, dispersant, hydrogen bondings, and shaky adsorbed water molecules. Besides, the lamellar thickness of CuP and the molecular size of lubricant both have a great influence on tribological properties. Here the lubrication mechanism based on interface Coulomb repulsion is proposed that may help broaden the scope of the exploration in low-friction nanomaterial design and new lubricant systems.
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