Frontiers in Plant Science
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2026
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IF 4.8
Lianjia Zhao,Xingang Li,Peiguo Wang,Weiwei Hao,Gan Li,Chunyan Li,Daolong Liao,Ke Wen
Soybean (Glycine max) plays a vital role in global food security and nutrition but faces increasing threats from soil salinization, an expanding environmental issue that affects approximately 1.38 billion hectares of land worldwide. Plants possess a diverse array of genes that facilitate adaptation to salt stress. Transcription factors are central regulators in mediating this response. They coordinate the physiological process that enables plants to adapt to high-salt environments by regulating the expression of downstream genes. The present study examines the function of the GmNAC018 gene in improving salt tolerance in soybean. We generated transgenic soybean plants with overexpression and observed improved germination, higher chlorophyll content, increased biomass, and lower sodium ion accumulation under salt stress compared to wild-type plants. The overexpression of GmNAC018 resulted in increased expression of key salt-tolerance genes, demonstrating its involvement in activation of the salt stress response pathway. Our results also suggest that GmNAC018 may be a key gene in regulating salt tolerance in soybean, providing theoretical molecular support for understanding the salt tolerance regulation mechanism in plants.
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Nature Communications
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2026
·Vol. 17
·期 1
·830
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IF 15.7
Xie Qian,Shen Yanni,Liang Jianhui,Fu Chang,Du Xiangfu,Huang Shuya,Song Bing,Chen Lei,Wang Yan
Autoimmune diseases (AIDs), such as psoriasis and rheumatoid arthritis, are driven by immune dysfunction, leading to chronic inflammation and tissue damage. Mesenchymal stromal cells (MSCs) possess immunomodulatory and tissue repair properties; however, the therapeutic applicability of MSCs faces limitations due to the low homing efficiency upon systemic infusion. Inspired by antibody-drug conjugates (ADC), here we develop an antibody-conjugated MSC-based drug delivery system (AcM-DDS) that combines CD4 monoclonal antibody-conjugated MSC (CD4-mBMSC) with liposome-encapsulated Cedirogant, a RORγt inverse agonist. AcM-DDS targets CD4⁺ T cells, key drivers of AID, and precisely delivers RORγt antagonists to suppress Th17-mediated inflammation. In mouse models of imiquimod-induced psoriasis and collagen-induced arthritis, AcM-DDS enhances MSC homing to inflamed tissues, reduces Th17 activity, lowers pro-inflammatory cytokine production, and preserves cartilage and bone integrity. Thus, our results indicate the promising applicability of our engineered platform for targeted immune modulation and provide support for the suitability of this therapeutic approach for the treatment of AIDs.
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FOOD RESEARCH INTERNATIONAL
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2026
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IF 8
Gongshuai Song,Tian Zhao,Yingcong Yang,Yangcheng Ge,Wei Tang,Taijiao Xiang,Danli Wang,Tinglan Yuan,Ling Li,Yong Cheng,Mengna Zhang,Jinyan Gong
The structural conversion of β-lactoglobulin (β-LG) into amyloid fibrils offers an effective strategy for developing functional biomaterials with enhanced physicochemical properties and functionalities. This study investigated the effect of cold plasma (CP) and ultrasound pretreatments on β-LG fibril (β-LGF) under acid-heat conditions (pH 2.0, 90 °C, 10 h) and explored the relationship between fibril structure and functional performance. Compared with the control, CP pretreatment significantly increased ThT fluorescence intensity by 1.9-fold, indicating enhanced fibril formation. Dynamic light scattering revealed that CP-treated fibrils (P-β-LGF) exhibited a reduced average particle size (183.6 ± 4.7 nm) and narrower distribution (PDI = 0.21) compared with ultrasound-treated (243.1 ± 6.5 nm) and control samples (278.4 ± 7.8 nm). FTIR and CD spectra confirmed the structural transition from α-helix to β-sheet, while SEM micrographs showed elongated, uniformly dispersed fibrils after CP pretreatment. Functionally, P-β-LGF exhibited the highest emulsifying activity index (51.2 m 2 /g), emulsion stability index (87.3%), and antioxidant activities, the emulsions stabilized by P-β-LGF-Dextran complex exhibited the superior thermal and ultraviolet stability of encapsulated lycopene. The highest retention rate (91.6%) was obtained in the emulsion. These findings demonstrated that CP treatment is an effective, green, and tunable strategy to regulate protein conformation and fibrillation kinetics, thereby enhancing the structural and functional properties of β-LG, providing new insights into the design of protein-based delivery systems for bioactive compounds.
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Small
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2026
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IF 12.1
Kai-Qi Jin,Qi-Yu Long,Jun-Lin Liu,Tian-Cai Sun,Kai-Kai Sheng,Yi Zhao,Wen-Ting Fan,Guo-You Huang,Wei-Hua Huang,Yan-Ling Liu
Acupuncture exerts its therapeutic effects through complex mechanical cues, including compressive forces from needle insertion and retention, and tensile stresses from extracellular matrix deformation during needle twisting. How these biomechanical stimuli regulate mast cell function at the molecular level remains poorly understood due to the lack of tools capable of replicating such multifaceted forces. Here, we report a magneto-responsive biosensor that enables multimodal simulation of stretching, compression, and combined loading in both static and cyclic modes. This allows for closely recapitulating the mechanical environment of mast cells during acupuncture, while simultaneously monitoring mechanically evoked cellular serotonin (5HT) release in real time. Using this sensor, we demonstrate that cyclic combined stimulation significantly amplifies cell responses by promoting both 5-HT release and intracellular biosynthesis. Furthermore, in vivo experiments at acupoints confirmed the “release-replenishment” phenomenon observed in vitro. Collectively, this study provides mechanistic insights into the molecular basis of acupuncture therapy and establishes a versatile tool for probing mechanobiological regulation in living systems.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 13.2
Yuze Luo,Ying Wang,Cheng Jin,Ke Yang,Zefeng Wang,Yinbang Zheng,Junqin Li,Chunlei Ou,Li Wang,Dinggeng He
The treatment of bacterial wound infection, particularly that caused by methicillin-resistant Staphylococcus aureus (MRSA), presents significant challenges owing to biofilm formation, multidrug resistance, and immune evasion. Here, we have rationally designed a multifunctional composite nanozyme (FTM) consisting of tannic acid (TA) and ferric ion (Fe 3+ ) coordination compound loaded with antibiotic drug methicillin (Meth), which is capable of realizing pH-responsive self-adaptive regulation of oxidative stress for enhancing the treatment of MRSA-infected wound. During the early stages of wound infection (pH < 6.5), FTM demonstrates significant peroxidase-like activity for effectively catalyzing the conversion of endogenous H 2 O 2 into highly toxic hydroxyl radical (•OH), which not only directly damages MRSA and eliminates its biofilm for chemodynamic therapy (CDT), but also disrupts the penicillin-binding protein 2a (PBP2a) to restore the sensitivity of MRSA to methicillin. Meanwhile, FTM exhibits strong near-infrared (NIR) absorption, achieving photothermal antibacterial therapy (PTT). While in the later stages (after complete elimination of MRSA), as the infectious microenvironment of wound becomes neutral (pH 7.4), FTM mainly reveals the superoxide dismutase (SOD)-like activity for scavenging excess reactive oxygen species (ROS), ultimately reducing oxidative stress and promoting tissue repair. In vitro and in vivo experiments show that the proposed FTM nanozyme with triple bactericidal modalities achieves satisfactory antibacterial and antibiofilm therapy and accelerates wound healing process. Consequently, by adapting to the dynamic changes of wound microenvironment, this multifunctional nanoplatform offers a promising strategy for treating drug-resistant wound infections.
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Journal of Pharmaceutical Analysis
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2026
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IF 8.9
Xiaohui Hu,Yingxia Tian,Qianping Chen,Yanan Guo,Pengfei Ji,Yixiao Tian,Shuangxi Qian,Guolin Chai,Fangdi Hu,Rong Shen,Degui Wang
Senescence is widely recognized as a key driving factor in the onset and progression of many chronic diseases. Although numerous animal studies have demonstrated that various drugs can delay senescence, their clinical application is severely limited due to significant toxic side effects. As a result, developing safer, natural anti-senescence agents has become an urgent priority. In this study, we identified mitochondrial dysfunction as a critical contributor to cellular senescence. In addition, we identified Shenrong Guben Huanshao Pill (SRW) as an effective agent that alleviates mitochondrial dysfunction and consequently delays cellular senescence by regulating mitochondrial metabolism. Mechanistically, SRW enhances mitochondrial function by upregulating the expression of NADH:ubiquinone oxidoreductase subunit V2 (NDUFV2), a core component of mitochondrial respiratory chain complex I. Simultaneously, SRW reduces lactate-induced mitochondrial dysfunction by regulating the glycolytic enzyme lactate dehydrogenase B (LDHB), thereby decreasing lactate accumulation. Collectively, our findings indicate that SRW alleviates cellular senescence by coordinately regulating NDUFV2 expression and LDHB-mediated lactate metabolism to improve mitochondrial function. In addition, several flavonoids in SRW were identified to possess potential anti-senescence activity. Molecular docking and cellular experiments confirmed that myricetin, luteolin, and gamma-mangostin effectively alleviate cellular senescence.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2026
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IF 6.1
Xiaotian Huang,Zuhong Lin,Denglong Lu,Haipu Li
Tetrabromobisphenol A (TBBPA), a prominent brominated flame retardant (BFR), is a toxic pollutant. In view of the structural similarity of TBBPA to thyroid hormones, we investigated the toxic effects of TBBPA on human thyroid follicular epithelial cells (Nthy-ori3–1) at concentrations ranging from 0.02 to 100 μM. The findings of this study demonstrated that a 24-hour exposure to 10 μM TBBPA resulted a reduction of cell viability to 50.9 %. Concurrently, intracellular reactive oxygen species (ROS) levels were significantly elevated, and oxidative stress-related proteins, particularly those involved in the NRF2/KEAP1 pathway, were upregulated in the cells. It is noteworthy that, while catalase (CAT) activity increased, T-SOD enzyme activity was inhibited. To better understand the mechanism underlying the interaction between TBBPA and antioxidant enzymes, multispectral methods and molecular docking techniques were employed. These analyses revealed that TBBPA binds to antioxidant enzymes and induces structural changes that may impair their function. Altered enzyme activity may lead to abnormal ROS accumulation and contribute to apoptosis. Furthermore, the interactions between TBBPA and Cu/Zn-SOD and CAT have been demonstrated to exacerbate this process. This study provides valuable insights into the molecular mechanisms underlying TBBPA-induced thyroid cell toxicity.
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JOURNAL OF CONTROLLED RELEASE
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2026
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IF 11.5
Ying-Tong Ye,Xi Xiao,Hong-Ying Xia,Jie Li,Zhen-Zhen Gong,Ai-Zheng Chen,Shi-Bin Wang,Ranjith Kumar Kankala
Despite the success of various intelligent materials in meeting the multiple needs of diabetic ulcer wounds (DUW) healing, it is often challenging to consider the entire healing process, from early antibacterial and anti-inflammatory effects to late matrix remodeling and angiogenesis characteristics, by releasing multiple active ingredients in stages and on demand. Herein, we designed a versatile bilayer (GCM/Clean-GDA/Cure) hydrogel dressing with light-controlled, spatiotemporal sequential release of MXene nanosheets (MX NSs)/nitric oxide (NO) gas for the healing of methicillin-resistant Staphylococcus aureus (MRSA) biofilm-infected DUWs. Initially, the lower GCM layer comprised carboxymethyl chitosan-gelatin and cerium oxide (CeO 2 )-decorated MX NSs (CO-MX), and the upper GDA layer consisted of dopamine-modified methacrylated gelatin (GelMA-DA) with L-arginine (L-Arg) as a precursor. Under NIR irradiation, the GCM hydrogel releases photothermal-induced CO-MX NSs as self-regenerating antioxidants, exhibiting 98.25 % inhibition against MRSA and 96.37 % inhibition against Pneumonia aeruginosa. The abundant oxygen vacancies and reversible Ce(III)/Ce(IV) redox pairs of the released Ce species from CO-MX NSs could attenuate inflammation and promote early macrophage polarization. The light-assisted gel–sol thermal transition of the lower GCM layer further exposes the upper GDA layer, providing a conducive microenvironment for angiogenesis and tissue remodeling. L-Arg in the GDA layer could enable light-controlled in situ release of NO gas activated by elevated reactive oxygen species (ROS), reaching 7.71 μM within 10 min of exposure to NIR light. Thus, the NIR-assisted bilayer treatment group achieved a 97.74 % wound closure rate. Finally, the transcriptomic analysis validated the activation of regenerative pathways and suppression of excessive inflammatory and metabolic stress signals. Together, the photo-controlled sequential release of MX NSs and NO gas from the bilayer could expand the application of light-responsive nanomaterials, providing an innovative therapeutic modality for in situ treatment of diabetic wounds infected by highly drug-resistant MRSA biofilms.
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Nature Communications
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2026
·Vol. 17
·期 1
·173
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IF 15.7
Luo Ruifeng,Liu Jinwei,Dai Linxin,Duan Tianshun,Zhang Qingwen,Gao Cheng,Wang Ruibing
Inflammatory bowel disease (IBD) involves elevated intestinal reactive oxygen species (ROS) and microbial imbalance. A key challenge is that current delivery systems cannot adequately protect active agents, such as antioxidants and probiotics, through the harsh gastric environment nor precisely deliver them to inflamed intestinal sites. Here, we present the EcN@PDA@HP biohybrid robot, composed of stress-trained microalgae, probiotics, and an intestinal braking system, enabling rapid gastric passage and precise retention in inflamed regions. Stress-trained Haematococcus pluvialis (HP) developed a thickened cell wall for gastric resilience, maintained flagella-driven motility, and enhanced astaxanthin (AST) production. Polydopamine (PDA)-coated Escherichia coli Nissle 1917 (EcN) anchors onto HP via host-guest interactions. In vivo, PDA preserves EcN activity and enables specific adhesion to inflamed sites. By combining antioxidizing AST and bacteriotherapy, EcN@PDA@HP effectively alleviates male murine IBD models via ROS scavenging and microbiota restoration, offering a promising strategy for diverse IBD conditions.
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ANALYST
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2026
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IF 3.3
Xueying Zhu,Shuo Tian,Shuyun Chen,Yunsen Wang,Dianping Tang
As a key alkaloid, the concentration of xanthine in the body often reflects various physiological or pathological states of the human body. However, conventional methods for xanthine detection often suffer from time-consuming procedures and a narrow linear range. The present study screened 30 nanozymes with varying Fe/S molar ratios using a hydrogen peroxide-3,3',5,5'-tetramethylbenzidine (H2O2-TMB) colorimetric system and identified FeS-loaded nitrogen-doped carbon nanozyme (Fe300S5-NC) as a highly efficient catalyst. Inspired by the biological metabolic pathway of xanthine, an XOD/Fe300S5-NC/TMB colorimetric system was constructed for the detection of xanthine. The constructed colorimetric sensing platform demonstrated facile operation and rapid response, with a linear xanthine detection range of 10–1000 μM and a detection limit of 0.97 μM. This work provides a viable strategy for designing artificial enzymes with high peroxidase-like activity, indicating potential for biosensing applications.
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JOURNAL OF ETHNOPHARMACOLOGY
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2026
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IF 5.4
Xing-lan Ye,Ling-shuai Zeng,Qiang Liu,Wen-cheng Xu,Hong Liu,Hong Cheng,Wen-xiao Zheng,Xiao-qin Wang,Xue Xue
Ethnopharmacological relevance Huaiqihuang granule (HQH) is a traditional Chinese herbal formula comprising Trametes robiniophila Murr. , Lycii Fructus ., and Polygonatum sibiricum Red. This granule can nourish Qi and Yin, improve blood circulation and remove blood stasis. HQH has been extensively used to improve immune-mediated kidney diseases, such as IgA vasculitis nephritis (IgAVN). However, its renal protective mechanism remains elusive. Aim of the study This study investigated whether HQH ameliorates IgAVN by modulating the AMPK/mTOR-mediated autophagy pathway. Materials and methods The IgAVN mouse model was established using gliadin sensitization and India ink-mediated reticuloendothelial blockade (n = 10/group). The model mice were then treated for 4 weeks with low/high-dose HQH (4/8 g/kg/day) or dexamethasone (2.5 mg/kg/day). For in vitro analyses, human mesangial cells (HMCs) were first stimulated with aggregated IgA1 (aIgA1, 25 μg/mL) and then treated with the optimal concentration of HQH-containing serum (40 %). Furthermore, biochemical indices assays, renal histopathology, and immunofluorescence analysis of IgA deposition were performed. Moreover, autophagy levels were evaluated by assessing the expression of autophagy-related proteins, MDC staining, and mCherry-EGFP-LC3 transfection. Western blot and immunofluorescence were carried out to assess the levels of the AMPK/mTOR pathway proteins and proliferation markers. Results The results showed that HQH treatment significantly reduced 24-h urinary protein and serum IgA levels, but improved renal histopathology in IgAVN mice. Furthermore, HQH activated autophagy by upregulating p-AMPKα, LC3-Ⅱ/LC3-Ⅰ, and Beclin1, while inhibiting p-mTOR/mTOR and p62. Moreover, in HMCs, 40 % HQH-containing serum optimally inhibited aIgA1-induced mesangial proliferation and increased autophagic levels, as evidenced by the quantitative increase in autophagy activity and mean fluorescence intensity of MDC. However, these effects were blocked by AMPK inhibition. Conclusion This study showed that HQH alleviates IgAVN by upregulating AMPK/mTOR-mediated autophagy, inhibiting mesangial cell proliferation, and mitigating renal injury. These findings suggest that HQH has therapeutic potential for IgAVN and link autophagy modulation to IgAVN treatment.
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DYES AND PIGMENTS
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2026
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IF 4.2
Jiahui Cai,Yuwei Du,Huimin Miao,Yingge Cong,Minghui Zan,Ruhong Yan,Li Li,Juan Yue
The overuse of tetracycline (TC) antibiotics has raised significant environmental and health concerns, while traditional detection methods face challenges in real-time monitoring and intracellular analysis. In this study, sulfur-doped carbon dots (S-CDs) were synthesized through a one-step hydrothermal method, using biotin and o -phenylenediamine as precursors. The resulting S-CDs exhibit three key functional properties: (1) a highly sensitive fluorescent quenching response to TC, with a detection limit of 76.6 nM and a linear range of 0–200 μM, driven by specific molecular interactions (inner filter effect and static quenching); (2) pH-dependent fluorescence signaling (pH 2.0–8.0) attributed to protonation of surface acid-base groups; and (3) selective lysosomal targeting, with a Pearson correlation coefficient of 0.95, confirmed by confocal microscopy in HeLa cells and zebrafish models. The S-CDs demonstrated excellent recoveries (97.07–107.99 %) in real food samples (milk, beef, and pork) and exhibited negligible cytotoxicity. This work effectively integrates environmental pollutant detection with intracellular microenvironment analysis, providing a versatile tool for investigating TC-induced lysosomal dysfunction and advancing food safety monitoring and biomedical imaging.
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