JOURNAL OF MOLECULAR LIQUIDS
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2023
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IF 6
Zhicheng Deng,Junhao Zhu,Yun Chen,Nanjing Zhong,Chao Huang,Yong Hu
A novel nanohybrid polysaccharide hydrogel was prepared using octenyl succinic anhydride/β-cyclodextrin nanoparticles (OS-β-CD NPs) crosslinked with polyvinyl alcohol (PVA) and κ-carrageenan (κ-CRG) via cascade self-assembly. FTIR and 1 HNMR confirmed that the formation of NPs/PVA/κ-CRG hydrogels was mainly by hydrogen bonds and electron forces. Results of SEM showed that the NPs/PVA/κ-CRG hydrogels had a well-developed porous structure with interconnected pores of about 4.22 μm. Measurements of rheological, swelling, and thermogravimetric revealed that the NPs/PVA/κ-CRG hydrogels had a good solid-like properties and enhanced mechanical strength due to the participation of OS-β-CD NPs in the formation of hydrogel network structure. In vitro release of the NPs/PVA/κ-CRG hydrogels loaded with vitamin E (VE) and proanthocyanidins (PC) simultaneously in PBS showed that the hydrogels could commendably control the explosive release of VE and PC and prolong release period during co-delivery. Moreover, the double active compounds loaded NPs/PVA/κ-CRG hydrogels were beneficial in enhancing synergistic antioxidant capacity.
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BIOLOGICAL TRACE ELEMENT RESEARCH
·
2026
·
IF 4.3
Liu Bai Han,Zhang Jin Ge,Peng Dong Qiao,Jin Yong Cheng
Copper is an essential trace element for beef cattle, yet its muscle developmental regulatory mechanism remains unclear. This study investigated the effects of copper sulfate (CuSO₄) on myogenic differentiation of bovine skeletal muscle-derived cells (BSMC) and the underlying molecular mechanisms. BSMC were isolated from the longissimus thoracis muscle of three 30-month-old crossbred steers, with each steer defined as an independent experimental unit corresponding to a separate biological replicate ( n = 3). Based on cell viability, CuSO₄ was used at 0, 0.1, 1, and 10 µM. All cell samples were harvested at differentiation day 6 (D6). The 0.1 µM CuSO₄ treatment during cell growth significantly promoted D6 myotube formation ( P = 0.049), and upregulated mRNA and protein expression of myogenic differentiation 1 (MyoD) ( P = 0.044, P = 0.030), as well as protein expression of hypoxia-inducible factor-1α (HIF-1α) ( P = 0.037) and β-catenin ( P = 0.050). During differentiation, 1 µM CuSO₄ significantly facilitated D6 myotube formation ( P = 0.0004), increased myogenic factor 5(Myf5) mRNA and protein expression ( P = 0.030, P = 0.006), and elevated HIF-1α ( P = 0.025) and β-catenin ( P = 0.004) protein levels, whereas Wnt10b protein expression remained unaltered ( P = 0.930). Single or combined pharmacological inhibition of HIF-1α and β-catenin markedly reversed copper’s pro-myogenic effect on BSMC. In conclusion, copper modulates BSMC myogenic differentiation via the HIF-1α/β-catenin axis by enhancing β-catenin protein stability independent of Wnt10b transcription. HIF-1α acts upstream to drive β-catenin, and a reciprocal regulatory relationship may exist in which β-catenin in turn helps sustain HIF-1α abundance, thereby supporting cascade signaling and downstream myogenic transcription. This study elucidates copper’s molecular mechanism governing muscle development and provides theoretical support for precise copper supplementation to improve beef production performance.
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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
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2015
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IF 3.447
Feifei Li,Guoli Wu,Huixia Zheng,Li Wang,Zhengbao Zhao
A glycoside prodrug of 4-aminosalicylic acid (4-ASA) with d -glucose was synthesized for targeted drug delivery to inflammatory bowel. The in vitro assessment of 4-aminosalicylic acid-β-O-glucoside (4-ASA-Glu) as a colon-specific prodrug was studied using colitis rat with the healthy one as control. The stability studies in aqueous buffers (pH 1.2, 6.8 and 7.4) indicated that 4-ASA-Glu was stable over a period of 12 h. The incubation of 4-ASA-Glu with cecal or colonic contents of healthy rats at 37 °C released 4-ASA in 77 or 80% of the dose in 12 h, respectively. The amount of 4-ASA liberated from the incubation of 4-ASA-Glu in cecal or colonic contents of colitis rats at 37 °C was 69 or 79% in 12 h respectively, while less than 9% 4-ASA was detected from the incubation of 4-ASA-Glu with the homogenates of stomach or small intestine . The curative effect of 4-ASA-Glu was evaluated in 2, 4, 6-trinitrobenzenesulfonic acid (TNBS) induced experimental colitis model in male Sprague–Dawley (SD) rats. It was found that 4-ASA-Glu possess significantly ameliorate effect than sulfasalazine , oral 4- and 5-aminosalicylic acid.
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BIOORGANIC & MEDICINAL CHEMISTRY
·
2018
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IF 2.881
Yifan Zhao,Feng Ye,Jian Xu,Qinghong Liao,Lei Chen,Weijia Zhang,Haopeng Sun,Wenyuan Liu,Feng Feng,Wei Qu
To develop potent multi-target ligands against Alzheimer's disease (AD), a series of novel bivalent β -carboline derivatives were designed, synthesized, and evaluated. In vitro studies revealed these compounds exhibited good multifunctional activities. In particular, compounds 8f and 8g showed the good selectivity potency on BuChE inhibition (IC 50 = 1.7 and 2.7 μM, respectively), A β 1-42 disaggregation and neuroprotection. Compared with the positive control resveratrol, 8f and 8g showed better activity in inhibiting A β 1-42 aggregation, with inhibitory rate 82.7% and 85.7% at 25 μM, respectively. Moreover, compounds 8e , 8f and 8g displayed excellent neuroprotective activity by ameliorating the impairment induced by H 2 O 2 , okadaic acid (OA) and A β 1-42 without cytotoxicity in SH-SY5Y cells. Thus, the present study evidently showed that compounds 8f and 8g are potent multi-functional agents against AD and might serve as promising lead candidates for further development.
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JOURNAL OF NATURAL PRODUCTS
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2019
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IF 4.257
Yong Ling,Yangyang Li,Rui Zhu,Jianqiang Qian,Ji Liu,Weijie Gao,Chi Meng,Jiefei Miao,Biao Xiong,Xiaodong Qiu,Changchun Ling,Hong Dai,Yanan Zhang
Naturally occurring β-carbolines are known to have antitumor activities but with limited effectiveness. In order to improve their efficacy, a series of new hydroxamic-acid-containing β-carbolines connected via a hydroxycinnamic acid moitey (12a–f) were developed to incorporate histone deacetylase (HDAC) inhibition for possible synergistic effects. When evaluated in in vitro assays, most of the analogues showed significant antitumor activities against four human cancer cells. In particular, 12b showed the highest cytotoxic potency of the series, including drug-resistant Bel7402 cells, but had minimal effect on normal hepatic LO2 cells. These compounds also showed excellent inhibitory effects against HDAC1/6, which appear to contribute greatly to their antiproliferative properties. Compound 12b enhanced the acetylation levels of histone H3 and α-tubulin and induced greater cancer cell apoptosis than the FDA-approved HDAC inhibitor SAHA by regulating expression of apoptotic proteins Bax, Bcl-2, and caspase 3. Importantly, 12b also induced a significant amount of autophagic flux activity in Bel7402 cells by increasing the expression of Beclin-1 and LC3-II proteins and decreasing that of LC3-I and p62. Finally, 12b significantly inhibited PI3K/Akt/mTOR signaling, an important cell-growth-promoting pathway aberrantly activated in many cancers. Together, the results suggest that these hydroxamic-acid-containing β-carboline derivatives may be new leads for the discovery of agents for the treatment of human carcinoma cancers.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2023
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IF 2.5
Chen Linlin,Song Jiaqi,Wang Ling,Hao Xi,Zhang Haipeng,Li Xintong,Wu Jiashu
Nitrite ( \(\mathrm{{NO}_2}^{-}\) ) is often added to food as a food additive, but the overuse of nitrite will pose a threat to human health, so it is important to establish a rapid detection method for nitrite in related food. In this work, gold nanoparticles (AuNPs) were synthesized by in situ reduction of methanobactin (Mb); it is a small bioactive peptide. HS-β-cyclodextrin (HS-β-CD) with supramolecular recognition function was coordinated with Mb/AuNPs by Au-S bond to form HS-β-CD@Mb/AuNPs. HS-β-CD@Mb/AuNPs were modified onto the surface of gold electrode by electrodeposition to successfully prepare the biosensor (HS-β-CD@Mb/AuNPs/Au). The HS-β-CD@Mb/AuNPs/Au was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), ultraviolet spectroscopy (UV), Fourier infrared spectroscopy (FTIR), fluorescence spectrometer (FL), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The established biosensor was successfully applied to the detection of nitrite in real samples, and the mechanism of NO 2 − oxidation was analyzed. The detection limit of the established detection system was as low as 0.013 μM in the linear range of 0.1–10,000 μM, the recoveries were 96.53–102.54%, and the selectivity of the sensor is demonstrated by anti-interference experiment. Also compared to the previously described electrochemical sensors, the newly developed ones have better sensitivity (50.7137 μA μM −1 cm −2 ) and selectivity. These results suggest that the work provides a new way for the accurate monitoring of nitrite in environmental and food safety.
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MOLECULAR BIOTECHNOLOGY
·
2023
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IF 2.6
Lv Chen-Guang,Cheng Yao,Zhang Lei,Wu Guo-Gang,Liang Chun-Yan,Tao Zuo,Chen Bo
BC (breast cancer) is the leading cause of cancer death in women. Exosome component 2 (EXOSC2), an RNA exosome component, is elevated in BC tissues and may relate to BC carcinogenesis. In this work, the high EXOSC2 expression was correlated with TNM (Tumor Node Metastasis) stage. Moreover, overexpression of EXOSC2 enhanced tumorigenic capacity of BC cells via facilitating cell proliferation and cell cycle progression, increasing migration and angiogenesis, as well as exacerbating xenograft formation in vivo. Whereas, EXOSC2 knockdown showed anti-cancer effects, including inhibition of cell proliferation and angiogenesis. Mechanistically, EXOSC2 activated the wnt/β-catenin pathway, which was also abolished by EXOSC2 knockdown. In addition, there were m6A methylation modification sites in the mRNA of EXOSC2. WTAP (Wilms tumor 1-associated protein) bound to EXOSC2 mRNA and increased its m6A methylation, resulting in extending the half-life of EXOSC2 mRNA. Luciferase data also confirmed that WTAP enhanced EXOSC2 mRNA stability through binding with the 3′-UTR containing m6A sites. Furthermore, WTAP silencing exhibited cancer-inhibiting effects on cell viability, cell cycle progression and tube formation, which was effectively reversed by EXOSC2 overexpression. In conclusion, our results demonstrate that EXOSC2 promotes the malignant behaviors of BC cells via activating the wnt/β-catenin pathway. In addition, EXOSC2 mediates the function of WTAP which contributes to the m6A modification of EXOSC2. Totally, this study suggested that EXOSC2 mediated the pro-tumor role of WTAP via activating the wnt/β-catenin signal.
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JOURNAL OF PHYSICS D-APPLIED PHYSICS
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2023
·Vol. 57
·期 1
·015104
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IF 3.4
Songhao Wu,Zichun Liu,Han Yang,Yeliang Wang
As a simple and effective method for improving the crystalline quality of epitaxial Ga2O3 film, post-thermal treatment has been identified as a competitive process involving crystal reconstruction accompanied by defect formation. In this study, β-Ga2O3 films grown on a c-sapphire substrate using low-pressure chemical vapor deposition were subjected to thermal treatment at 1000 °C in air for various duration to investigate the effects of treatment time on the films. The full width at half maximum (FWHM) of x-ray rocking curves initially decreased from 1.62° to 0.98° with increasing treatment time up to 5 h, indicating improved crystallinity. This improvement is likely a result of the reduced angle between Ga2O3 grains and the reconstructed Ga2O3 lattice, oriented towards the (−201) plane due to the thermal treatment, as observed in the transmission electron microscope and electron back-scattering diffraction results. However, under 7 h of treatment, the crystallinity of Ga2O3 degraded, as evidenced by an increased FWHM, as well as by x-ray photoelectron spectroscopy, photoluminescence, and time-of-flight secondary ion mass spectrometry results. This degradation can be attributed to the presence of massive oxygen vacancies and the substitutional incorporation of nitrogen into oxygen sites (NO), resulting in defects.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
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IF 5.455
Guochao Yang,Lingtao Wang,Haoxi Jiang
The conversion of ethanol to butadiene catalyzed by modified β zeolites has attracted wide attention. The construction of intracrystalline mesoporosity in β zeolite is a challenging way to improve the catalytic activity. In this work, a surfactant-template strategy was used to construct intracrystalline mesoporosity successfully in the alkali treatment of β zeolite using long-chain quaternary ammonium salt with different alkyl chain length (C10~C16) as the template. The intracrystalline pore size can be adjusted by varying the surfactant alkyl chain length (C10~C16) (1.9–3.4 nm). To get more information about this process, CTAB was chosen as the template in further investigations. The mesoporosity can be generated within 1 h. The BET surface area can reach up to 600 m 2 /g while the pore volume can reach up to 0.568 cm 3 /g, which are 1.23 and 1.5 times that of the parent zeolite, respectively. The template action of CTA + is essential for forming mesoporosity, and CTA + interacts with the zeolite during the alkali treatment process, protects the zeolite framework from being etched, and does not form a separated mesoporous phase. To demonstrate the effect of intracrystalline mesoporosity on the catalytic performance, Zr-β zeolite was prepared and used as the catalyst for ethanol-acetaldehyde conversion. The hierarchical Zr-β zeolite was prepared using a dealumination-metallization strategy, and both the zeolite crystallinity and the mesoporosity were preserved in this process. The total conversion increased by 10%, while the BD selectivity was well maintained. The catalytic performance showed that the introduction of mesoporosity effectively mitigated decreasing conversion while preserving BD selectivity.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2021
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IF 4.539
Shuai Lin,Changjun Zou,Hao Liang,Hong Peng,Ya Liao
β-cyclodextrin was covalently grafted onto the surface of magnetic multi-walled carbon nanotubes to synthesize a novel composite material (denoted as β-CD@Fe 3 O 4 /MWCNT). The structure of the magnetic material was characterized by using Fourier transform infrared (FT-IR spectroscopy), Raman spectroscopy , X-ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), vibrating sample magnetometer (VSM), and specific surface area (BET). The results from SEM showed that the dispersion of carbon nanotubes was better after β-cyclodextrin was compounded. TGA indicated that about 7.1% of the β-cyclodextrin was grafted onto the surface of the magnetic carbon nanotubes. In addition, β-CD@Fe 3 O 4 /MWCNT had larger specific surface area and pore volume . The adsorption of nickel ions on β-CD@Fe 3 O 4 /MWCNT was affected by pH, temperature and adsorption time. The pseudo-second-order kinetic model and Langmuir model describe the adsorption process well. Thermodynamic data showed that the adsorption process was spontaneous and exothermic, and the maximum adsorption amount of nickel ions (Ni 2+ ) on the β-CD@Fe 3 O 4 /MWCNT was 103 mg g −1 at room temperature . Moreover, regeneration experiments showed that the adsorbent still maintained a high adsorption capacity after 5 cycles. On this basis, the interaction mechanism between magnetic nanoparticles and Ni 2+ was discussed. In summary, as an environmentally friendly adsorbent, β-CD@Fe 3 O 4 /MWCNT has the potential to remove nickel ions from wastewater.
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Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2021
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IF 3.057
Qinqin Xu,Zikai Liu,Chen Yan,Runhua Lu,Wenfeng Zhou
In this study, a novel sorbent, 1-octyl-3-methylimidazolium hexafluorophosphate functionalised magnetic poly β-cyclodextrin, was successfully synthesised and applied to magnetic solid-phase extraction for the determination of pyrethroids in tea infusions. The sorbent was characterised by transmission electron microscopy, energy-dispersive spectroscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, vibrating sample magnetometer and Brunauer–Emmett–Teller measurement. All factors affecting extraction efficiency, such as sorbent amount, extraction time, ionic strength and desorption conditions, were optimised individually. Under the chosen conditions, wide linearity (2.5–500 μg L−1) with determination coefficients ranging from 0.9995 to 0.9999, low limits of detection of 0.32–0.54 μg L−1 and good precision (intra-day: 2.6–7.0%; inter-day: 3.5–7.6%) were achieved for four pyrethroids in tea infusions. The relative recoveries of target analytes in real tea infusion samples were from 70% to 101% with relative standard deviations lower than 9.1%. We conclude that the proposed method is promising in the detection of pyrethroids in tea infusions.
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ENVIRONMENTAL POLLUTION
·
2021
·
IF 8.071
Shibin Qin,Shihua Qi,Xiaoshui Li,Qiuyun Shi,Huan Li,Xiaoxuan Mou,Yuan Zhang
The rapid determination of the bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soils is challenging due to their slow desorption rates and the insufficient extraction efficiency of the available methods. Herein, magnetic poly(β-cyclodextrin) microparticles (Fe 3 O 4 @PCD) were combined with hydroxypropyl-β-cyclodextrin (HPCD) or methanol (MeOH) as solubilizing agents to develop a rapid and effective method for the bioaccessibility measurement of PAHs. Fe 3 O 4 @PCD was first validated for the rapid and quantitative adsorption of PAHs from MeOH and HPCD solutions. The solubilizing agents were then coupled with Fe 3 O 4 @PCD to extract PAHs from soil-water slurries, affording higher extractable fractions than the corresponding solution extraction and comparable to or higher than single Fe 3 O 4 @PCD or Tenax extraction. The desorption rates of labile PAHs could be markedly accelerated in this process, which were 1.3–12.0 times faster than those of single Fe 3 O 4 @PCD extraction. Moreover, a low HPCD concentration was sufficient to achieve a strong acceleration of the desorption rate without excessive extraction of the slow desorption fraction. Finally, a comparison with a bioaccumulation assay revealed that the combination of Fe 3 O 4 @PCD with HPCD could accurately predict the PAH concentration accumulated in earthworms in three field soil samples, indicating that the method is a time-saving and efficient procedure to measure the bioaccessibility of PAHs.
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