JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
He Zhu,Chentao Ding,Yunbing Tang,Jingyi Zheng,Shaowei Wang,Zhongyao Ji,Yang Zhu,Ren-shan Ge,Huitao Li
Parabens are preservatives used in personal care products, cosmetics, and pharmaceuticals. Steroid 5α-reductase 1 (SRD5A1) catalyzes the conversion of testosterone to dihydrotestosterone and is present in the brain, contributing to neurosteroid production. This study aimed to assess the effects of nine paraben preservatives on SRD5A1 in human SF126 glioblastoma cell and rat brain microsomes, particularly focusing on dihydrotestosterone production in SF126 cells. The results showed that methyl, ethyl, propyl, butyl, hexyl, heptyl, nonyl, phenyl, and benzyl paraben inhibited human SRD5A1, with nonylparaben having the strongest effect (7.59 μM). Additionally, kinetic analysis indicated that parabens acted as mixed/noncompetitive inhibitors, leading to a significant decrease in dihydrotestosterone production in SF126 cells. While rat SRD5A1 exhibited lower sensitivity to parabens, docking analysis revealed that parabens bind to the NADPH-binding site of both human and rat SRD5A1. In conclusion, these results highlight the inhibitory effects of paraben preservatives on SRD5A1 and elucidate their binding mechanisms, underscoring their role in hormone production.
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高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2025
·
IF 5.6
Lei Wang,Yufeng Bai,Dan Ma,Wenxiu Liu,Zhiyu Shi,Chunnan Liu,Meng Zhao,Huiying Wang,Liying Luo,Jinyu Chi
Hypertension is a risk factor for cardiovascular diseases, primarily through its induction of pathological vascular and ventricular remodeling. TEA domain transcription factor 3 (TEAD3) is a transcription factor highly expressed in myocardial tissues. PDZ domain-containing 1 (PDZK1) has been reported to protect blood vessels. This study discovered decreased expression of TEAD3 and PDZK1 in the aortic tissues of spontaneously hypertensive rats (SHRs). TEAD3 overexpression in SHR vascular smooth muscle cells (VSMCs) driven by the SM22α promoter was achieved through adeno-associated virus delivery. TEAD3 overexpression alleviated aortic remodeling by reducing elastic fiber and collagen deposition. This improvement in vascular structure attenuated hypertension. Subsequently, ventricular remodeling was alleviated by reducing periaortic myocardial fibrosis and left ventricular posterior wall thickness. To elucidate the underlying mechanisms, we overexpressed TEAD3 or PDZK1 in SHR-derived VSMCs via adenoviral infection. Both interventions suppressed VSMC proliferation and migration. Crucially, TEAD3 overexpression upregulated PDZK1 expression, and DNA pull-down assays confirmed direct binding of TEAD3 protein to the PDZK1 promoter. PDZK1 knockdown abolished the anti-proliferative and anti-migratory effects of TEAD3. Further analysis suggested that PDZK1 exerted its protective role by inhibiting the phosphoinositide-3-kinase adaptor protein 1-mediated PI3K/Akt pathway. In conclusion, this study reveals that TEAD3-PDZK1 axis attenuates the abnormal proliferation and migration of VSMCs, which ameliorates aortic and left ventricular remodeling in hypertensive conditions. These findings establish a molecular basis for developing targeted therapies against hypertension-induced cardiovascular remodeling.
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高影响因子
ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Xiang Li,Ping Zhang,Rui Wang,Meng Zhu,Xiaobo Gong,Yucheng Liu
The degradation of separation performance caused by the high abrasion of the continuous separation process puts forward more requirements for the adaptability and durability of the oil-water separation membranes. Herein, inspired by the mortise and tenon (MT) structures of ancient Chinese wooden frame buildings, a superhydrophilic and underwater superoleophobic stainless steel mesh (SSM) membrane with HKUST-1-copper hydroxide (CuOH) interlocking micro-nanostructures (HKUST-1/CuOH) was prepared via electrodeposition and hydrothermal growth techniques. The homogeneous growth of HKUST-1 and CuOH with superhydrophilicity and MT structures provides ultral wettability and durability for the HKUST-1/SSM/CuOH membrane. The optimal HKUST-1/SSM/CuOH membrane exhibited a water contact angle of 0° and an underwater oil contact angle of 156°. The flower-like microstructures improve acid and alkali tolerance, organic solvent tolerance, NaCl solution tolerance, thermal resistance, and UV aging performances. In addition, the HKUST-1 and CuOH coatings showed superior and durable antibacterial performance of the HKUST-1/SSM/CuOH membrane. This membrane with an MT structure demonstrated excellent reusability, showing a water flux of 23818 L·m –2 ·h –1 and UWOCA of 152.09° after 20 cycles. The proposed method is a promising candidate for unrelenting superhydrophobicity and high permeability in oily water treatment.
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高影响因子
PHYTOMEDICINE
·
2025
·
IF 8.3
Jie Gao,Jin Yan,Xingwang Zu,Yanyan Xu,Shilong Wang,Yue Li,Zhenying Zhao,Yuanyuan Hou,Gang Bai
Background In the innate immune system, damage-associated molecular patterns (DAMPs) released by damaged cells, such as peroxiredoxin 1 (PRDX1), interact with Toll-like receptor 4 (TLR4), exacerbating inflammation and tissue injury. Ferulic acid (FA), a dietary and herbal phenolic compound, exhibits notable anti-inflammatory properties. However, the precise anti-inflammatory mechanisms of FA are not fully understood. Purpose The objective of this study was to elucidate mechanisms underlying the anti-inflammatory effects of FA. Methods Lipopolysaccharide (LPS) was intratracheally instilled to establish a mouse model of LPS-induced pneumonia. Different doses of FA were injected intraperitoneally, and their anti-inflammatory effects were evaluated. An alkynyl-modified FA probe was used in conjunction with various chemobiological strategies to explore the localization, capture, and identification of FA targets. The pharmacophore of FA and its mechanisms of interaction with its target or pathway were further validated using rigorous biochemical assays and comprehensive transcriptomic profiling. The proposed mechanism of FA against systemic inflammation was validated in mice administered LPS intraperitoneally. Results FA alleviated LPS-induced pulmonary inflammation in mice by selectively targeting macrophages. Subsequently, PRDX1 was identified as an irreversible FA-binding target. Mechanistic investigations revealed that α,β-unsaturated ketone in FA serves as a critical pharmacophore that covalently binds to the Cys173 residue of PRDX1. This covalent binding event effectively suppressed PRDX1 dimerization, resulting in reduced PRDX1 secretion. The co-localization assay demonstrated that FA reduced TLR4-binding PRDX1 in LPS-treated RAW264.7 cells. Transcriptomic analysis indicated that NF-κB and TNF signaling pathways, downstream of TLR4 signaling, were involved in the FA-mediated anti-inflammatory effects. Reduction of TLR4 activation caused by FA treatment decreased levels of downstream inflammatory cytokines in LPS-treated RAW264.7 cells. Finally, reduced co-localization of TLR4 and PRDX1 by FA was confirmed in the lung slices of mice with LPS-induced intratracheal inflammation. FA also reduced PRDX1 dimerization and mitigated inflammation in mice administered LPS intraperitoneally. Conclusion Our study elucidated a novel mechanism in which FA covalently binds to the Cys173 residue of PRDX1, suppressing its dimerization and secretion and thereby alleviating inflammation by modulating the PRDX1/TLR4 signaling pathway. Our findings redefine plant phenylacrylic acids as covalent modulators of DAMPs, with therapeutic potential for ameliorating inflammation beyond conventional antioxidants.
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高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2025
·
IF 2.2
XiaoRong Meng,Dandan Meng,Chenhui Xiang,Lingling Pu,Xiaofei Yin,Jing Yang
Objective Postmenopausal osteoporosis (PMOP) is a prevalent metabolic bone disorder characterized by decreased bone mineral density and an increased risk of fractures. This study aims to investigate the molecular mechanisms underlying the therapeutic effects of Apelin-13 in PMOP. Methods A PMOP model was established using mouse embryonic pre-osteoblast MC3T3-E1 cells treated with dexamethasone (DEX). A variety of experimental techniques—including MTT assays, Western blotting, flow cytometry, immunofluorescence staining, mitochondrial membrane potential assays, and ELISA—were employed to elucidate the potential mechanisms through which Apelin-13 exerts its therapeutic effects. Results Apelin-13 significantly enhanced osteoblast activity and effectively reversed the inhibitory effects of DEX on cell proliferation. It suppressed apoptosis by modulating the expression of apoptosis-related proteins. Furthermore, Apelin-13 promoted osteogenic differentiation, upregulated osteogenic protein expression, increased alkaline phosphatase (ALP) activity, and facilitated calcium nodule formation. It also improved mitochondrial function by reducing levels of reactive oxygen species (ROS) and malondialdehyde (MDA), boosting antioxidant enzyme activity, and activating the Nuclear factor erythroid 2-related factor 2 (NRF2) signaling pathway. Critically, Apelin-13 was found to regulate the OPG/RANK/RANKL/IGF-1 signaling axis, and its pro-osteogenic effects were significantly enhanced when used in combination with SPA0355. Conclusion Apelin-13 exhibits promising therapeutic potential for PMOP in in vitro models by modulating key processes related to bone metabolism. It achieves this by attenuating mitochondrial oxidative phosphorylation and regulating the OPG/RANK/RANKL/IGF-1 pathway, thereby laying a foundation for future in vivo studies and clinical applications.
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PubMed
高影响因子
Korean Journal of Pain
·
2024
·
IF 3.4
Kaiwen Lin,Datian Fu,Zhongtao Wang,Xueer Zhang,Canyang Zhu
Background Galangin, commonly employed in traditional Chinese medicine for its diverse medicinal properties, exhibits potential in treating inflammatory pain. Nevertheless, its mechanism of action remains unclear. Methods Mice were randomly divided into 4 groups for 7 days a normal control group, a galangin-treated (25 and 50 mg/kg), and a positive control celecoxib (20 mg/kg). Analgesic and anti-inflammatory effects were evaluated using a hot plate test, acetic acid-induced writhing test, acetic acid-induced vascular permeability test, formalin-induced paw licking test, and carrageenan-induced paw swelling test. The interplay between galangin, transient receptor potential vanilloid 1 (TRPV1), NF-κB, COX-2, and TNF-α proteins was evaluated via molecular docking. COX-2, PGE2, IL-1β, IL-6, and TNF-α levels in serum were measured using ELISA after capsaicin administration (200 nmol/L). TRPV1 expression in the dorsal root ganglion was analyzed by Western blot. The quantities of substance P (SP) and calcitonin gene-related peptide (CGRP) were assessed using qPCR. Results Galangin reduced hot plate-induced licking latency, acetic acid-induced contortions, carrageenan-triggered foot inflammation, and capillary permeability in mice. It exhibited favorable affinity towards TRPV1, NF-κB, COX-2, and TNF-α, resulting in decreased levels of COX-2, PGE2, IL-1β, IL-6, and TNF-α in serum following capsaicin stimulation. Galangin effectively suppressed the upregulation of TRPV1 protein and associated receptor neuropeptides CGRP and SP mRNA, while concurrently inhibiting the expression of NF-κB, TNF-α, COX-2, and PGE2 mRNA. Conclusions Galangin exerts its anti-inflammatory pain effects by inhibiting TRPV1 activation and regulating COX-2, NF-κB/TNF-α expression, providing evidence for the use of galangin in the management of inflammatory pain.
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高影响因子
INTEGRATED FERROELECTRICS
·
2014
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IF 0.371
Mi Ouyang,Pingjing Wang,Zhiyan Fu,Cheng Zhang
A novel copolymer based on bis(4-(1H-pyrrol-1-yl)phenyl)methane (BPM) and 3,4-ethylenedioxythiophene (EDOT) is successfully synthesized and characterized through electrochemical methods. The polymer film exhibits three distinct colors (khaki, yellow green, midnight blue) under different potentials, reasonable optical contrast (10% at 422 nm, 5% at 1100 nm) and fast swiching time (2.6 s at 422 nm, 2.2 s at 1100 nm). Beside, SEM images illustrates that the copolymer film presents a much smoother surface than that of the respective homopolymers, which means that the copolymer film have great potential in electrochromic device fabrication.
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高影响因子
Postepy Dermatologii i Alergologii
·
2025
·Vol. 42
·期 4
·398-406
·
IF 1.7
Bei Zhao,Yinghua Liu,Xuemei Tang,Shi Cheng
Introduction:The effectiveness of chemotherapy in treating melanoma is limited due to drug resistance. Previous studies have shown that SENP1 (Sentrin/SUMO-specific protease 1) is related to the tumour hypoxic microenvironment, tumorigenesis and metastasis.Aim:This study aimed to investigate its roles in drug resistance of melanoma.Material and methods:Originally, a concentration of 2 µg/ml dacarbazine (DTIC) was employed in the treatment of A375 and M14 cell lines for a duration of 24 h. Subsequently, the cells were transferred to fresh medium and allowed to proliferate until reaching 80% of their maximum density. This treatment cycle was then repeated for a total of 10 days, following which the DTIC concentration was doubled. The establishment of drug-resistant cell lines for both A375 and M14 occurred after 8 months of sustained and continuous treatment. The expression level of SENP1 was monitored monthly using real-time reverse transcriptase-polymerase chain reaction (RT-qPCR), with a fold change above 1.5 compared to the untreated condition considered as significant.Results:Finally, the study found that SENP1 was up-regulated by about 2.2-1.7 times in the drug-resistant cells. In addition, overexpression of SENP1 in normal A375 cells improved cell viability against DTIC. The study also found that Yes-associated protein (YAP) could form protein condensates in the cytoplasm while its expression was enhanced by SENP1-mediated deSUMOylation.Conclusions:This study suggests that there is a positive correlation between the ubiquitin-specific protease SENP1 and drug resistance in melanoma. Its up-regulation may lead to changes in the deSUMOylation of YAP, activate the Hippo signalling pathway, and increase the resistance of melanoma to DTIC.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 20
·9196-9207
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IF 2.7
Yaowei Hao,Qiaojun Nie,Xiaocao Cao,Jiafan Zheng,Kai Zhang,Jun Zheng,Haodong Wang,Yafang Zhao,Jiayi Liu,Zhiguang Lin,Ming Zhang,Zhongrong Shen
All-solid-state fluoride-ion batteries (FIBs) with a high theoretical energy density have attracted considerable attention due to their enhanced safety features and superior capacity. The selection of solid electrolyte materials with enhanced fluoride-ion conductivity is crucial for the successful implementation of high fluoride-ion energy storage in realistic scenarios. In this study, heterogeneous solid fluoride-ion electrolytes with different ratios of (LaF3)1−x(PbF2)x are investigated. In contrast to other research efforts focusing on the development of single-phase, single-lattice-defect solid-state electrolytes, the (LaF3)1−x(PbF2)x material incorporates two phases with different defects, namely fluoride vacancies and mobile fluoride ions. This unique combination of defects results in a significant enhancement in conductivity while maintaining their coexistence. The crystal gaps are filled by the orthorhombic phase, which is conductive, resulting in a reduction of the contact resistance due to the uniform distribution of the three crystal phases and the heterointerface between the hexagonal phase and the cubic phase. The multi-component electrolyte (LaF3)0.4(PbF2)0.6 exhibits the best performance among the samples tested. The electrolyte exhibits a high fluoride-ion conductivity, with a value of 2.78 × 10−4 S cm−1 at 120 °C and 6.37 × 10−6 S cm−1 at room temperature. The electronic conductivity is negligible with a value of 5.48 × 10−8 S cm−1. Furthermore, the electrolyte has an electrochemical stability window of 1.85 V vs. Sn/Sn2+. Due to its favorable electrochemical properties, this material has considerable potential as a solid-state fluoride-ion electrolyte.
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高影响因子
Nano Research
·
2023
·Vol. 16
·期 12
·13366-13374
·
IF 9.9
Lin Chaoxin,Kim Kumchol,Wang Zuochao,Yan Zhuang,Tang Zhiyong,Liu Yaling
Integration and synergy of the unique functions of different components have been developed into one of the most convenient and effective ways to construct the composite advanced materials with collective properties and improved performances. In this work, the mace-like tetrathiafulvalene-tetracyanoquinodimethane (TTF-TCNQ)/HKUST-1 composite structures with single single-crystalline TTF-TCNQ submicrorods covered by ordered HKUST-1 nanosheet arrays were successfully constructed by an efficient TTF-TCNQ seed-mediated growth approach. Impressively, thanks to the synergetic and complementary effects between TTF-TCNQ and HKUST-1, the sensors based on such mace-like TTF-TCNQ/HKUST-1 composite structures not only displayed an experimental detection limit of 10 part per billion (ppb) for NO 2 detection, but also exhibited outstanding selectivity even if the concentration of the interfering gases was 10 times that of NO 2 . Meanwhile, good reproducibility and rapid response were also achieved. This work opens the avenue for creation of novel high-performance sensing materials for application in gas sensing.
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JOURNAL OF HAZARDOUS MATERIALS
·
2017
·
IF 6.065
Jianxian Zeng,Zhihui Dong,Zhe Zhang,Yuan Liu
A surface-grafted imprinted ceramic membrane (IIP-PVI/CM) for recognizing molybdate (Mo(VI)) anion was prepared by surface-initiated graft-polymerization. Firstly, raw alumina ceramic membrane (CM) was deposited with SiO 2 active layer by situ hydrolysis deposition method. Subsequently, γ-methacryloxy propyl trimethoxyl silane (MPS) was used as a coupling agent to introduce double bonds onto the SiO 2 layer (MPS-CM). Then, 1-vinylimidazole (VI) was employed as a functional monomer to graft-polymerization onto the MPS-CM (PVI-CM). During the graft-polymerization, the influence factors of grafting degree of PVI were investigated in detail. Under optimum conditions (monomer concentration 20 wt%, temperature 70 °C, initiator amount 1.1 wt% and reaction time 8 h), the grafting degree of 20.39 g/100 g was obtained. Further, Mo(VI) anion was used as a template to imprint in the PVI-CM by employing 1,6-dibromohexane as a cross-linking agent, and then Mo(VI) was removed, obtaining the IIP-PVI/CM with many imprinted cavities for Mo(VI). Thereafter, static adsorption and dynamic separation properties of IIP-PVI/CM for Mo(VI) were studied. Results indicate that IIP-PVI/CM shows a specific selectivity for Mo(VI) with the adsorption capacity of 0.69 mmol/100 g, and the selectivity coefficient of IIP-PVI/CM is 7.48 for molybdate to tungstate anions. During the dynamic separation, IIP-PVI/CM has also good selectivity for separation of Mo(VI) and W(VI) anions.
研究领域:
环境化学
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材料科学
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分离科学
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高分子化学
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无机分析化学
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高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2016
·Vol. 134
·期 1
·
IF 1.866
Zhaowei Wang,Zhixia Wen,Xing Li,Junwei Yu,Xiaoxuan Liu
The preparation of the high cis −1,4-polyisoprene by Ziegler-Natta catalysis system was studied. The effect of Al-Ti catalysts modified by ethers with different structures which are different electron donor reagent on polymerization of isoprene has been mainly investigated. By the measurement method of the monomer conversion, FTIR, and 1 H NMR spectroscopy, the influence of, respectively, added diphenyl ether, anisole, dibutyl ether, or methyl tert -butyl methyl ether as a third active component on the heterogeneous TiCl 4 -Al( i -Bu) 3 -ether catalyst activity and microstructure of synthetic polyisoprene was analyzed. By the adding of diphenyl ether or dibutyl ether, the process of prefabricated heterogeneous catalyst is quickly and catalyst particle quantity is large. The polymerization conversion is high and the microstructure cis −1,4 content of the resulting polymer can reach 92%. But Al( i -Bu) 3 added anisole or methyl tert -butyl methyl ether hard to cooperate with TiCl 4 . © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133 , 44357.
研究领域:
高分子化学
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催化化学
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功能材料
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合成高分子
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配位聚合
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高影响因子
危险品化学品经营许可证（带存储）