Nature Communications
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2025
·Vol. 16
·期 1
·1-14
·
IF 15.7
Gong Xu,Liu Shan,Xia Bozhang,Wan Yichen,Zhang Shuyi,Zhang Baoyan,Wang Zehao,Chen Junge,Xiao Fei,Liang Xing-Jie,Yang Yun
Genetically engineered commensal bacteria are promising living drugs, however, their therapeutic molecules are frequently confined to their colonization sites. Herein, we report an oral protein delivery technology utilizing an engineered bacterial type zero secretion system (T0SS) via outer membrane vesicles (OMVs). We find that OMVs produced in situ by Escherichia coli Nissle 1917 (EcN) can penetrate the intact gut epithelial barrier to enter the circulation and that epithelial transcytosis involves pinocytosis and dynamin-dependent pathways. EcN is engineered to endogenously load various enzymes into OMVs, and the secreted enzyme-loaded OMVs are able to stably catalyze diverse detoxification reactions against digestive fluid and even enter the circulation. Using hyperuricemic mice and uricase delivery as a demonstration, we demonstrate that the therapeutic efficacy of our engineered EcN with a modified T0SS outperforms that with a direct protein secretion apparatus. The enzyme-loaded OMVs also effectively detoxify human serum samples, highlighting the potential for the clinical treatment of metabolic disorders.
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JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-15
·
IF 12.6
Wang Xingyi,Fang Hanyi,Hu Wenzhu,Feng Yuan,Zhou Zhangyongxue,Hu Mengyan,Jiang Dawei,Zhang Yongxue,Lan Xiaoli
Limited treatment response and inadequate monitoring methods stand firmly before successful immunotherapy. Recruiting and activating immune cells in the hypoxic tumor microenvironment is the key to reversing immune suppression and improving immunotherapy efficacy. In this study, biomimetic oxygen-delivering nanoparticles (CmPF) are engineered for homologous targeting and hypoxia alleviation within the tumor environment. CmPF targets the tumor microenvironment and delivers oxygen to reduce hypoxia, thereby promoting immune cell activity at the tumor site. In addition, granzyme B-targeted positron emission tomography (PET) imaging is employed to monitor immune cell activity changes in response to immunotherapy efficacy in vivo. The combination of CmPF with carboplatin and PD-1 inhibitors significantly suppresses tumor growth by 2.4-fold, exhibiting the potential of CmPF to enhance the efficacy of immunotherapy. Immunohistochemistry further confirms increased expression of key immune markers, highlighting the reprogramming of the tumor microenvironment. This study demonstrates that hypoxia alleviation enhances tumor immunotherapy efficacy and introduces a non-invasive PET imaging method for dynamic, real-time assessment of therapeutic response.
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Nanoscale Research Letters
·
2018
·Vol. 13
·期 1
·1-10
·
IF 3.125
Liang Chunyong,Luo Yongchao,Yang Guodong,Xia Dan,Liu Lei,Zhang Xiaomin,Wang Hongshui
Biodegradable porous biomaterial scaffolds play a critical role in bone regeneration. In this study, the porous nano-hydroxyapatite/collagen/poly(lactic-co-glycolic acid)/graphene oxide (nHAC/PLGA/GO) composite scaffolds containing different amount of GO were fabricated by freeze-drying method. The results show that the synthesized scaffolds possess a three-dimensional porous structure. GO slightly improves the hydrophilicity of the scaffolds and reinforces their mechanical strength. Young’s modulus of the 1.5 wt% GO incorporated scaffold is greatly increased compared to the control sample. The in vitro experiments show that the nHAC/PLGA/GO (1.5 wt%) scaffolds significantly cell adhesion and proliferation of osteoblast cells (MC3T3-E1). This present study indicates that the nHAC/PLGA/GO scaffolds have excellent cytocompatibility and bone regeneration ability, thus it has high potential to be used as scaffolds in the field of bone tissue engineering.
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Nature Communications
·
2018
·Vol. 9
·期 1
·1-7
·
IF 12.353
Ding Li,Wei Yanying,Li Libo,Zhang Tao,Wang Haihui,Xue Jian,Ding Liang-Xin,Wang Suqing,Caro Jürgen,Gogotsi Yury
Molecular sieving membranes with sufficient and uniform nanochannels that break the permeability-selectivity trade-off are desirable for energy-efficient gas separation, and the arising two-dimensional (2D) materials provide new routes for membrane development. However, for 2D lamellar membranes, disordered interlayer nanochannels for mass transport are usually formed between randomly stacked neighboring nanosheets, which is obstructive for highly efficient separation. Therefore, manufacturing lamellar membranes with highly ordered nanochannel structures for fast and precise molecular sieving is still challenging. Here, we report on lamellar stacked MXene membranes with aligned and regular subnanometer channels, taking advantage of the abundant surface-terminating groups on the MXene nanosheets, which exhibit excellent gas separation performance with H 2 permeability >2200 Barrer and H 2 /CO 2 selectivity >160, superior to the state-of-the-art membranes. The results of molecular dynamics simulations quantitatively support the experiments, confirming the subnanometer interlayer spacing between the neighboring MXene nanosheets as molecular sieving channels for gas separation.
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MICROCHIMICA ACTA
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2018
·Vol. 185
·期 2
·1-10
·
IF 5.705
Wang Yao,Ma Ruiyang,Xiao Ruobai,Hao Lin,Wu Qiuhua,Wang Chun,Wang Zhi
A porous material (polytriphenylamine; PTPA) was synthesized by using triphenylamine as the monomer and dimethoxymethane as the cross-linker. PTPA was characterized by Fourier infrared spectrometry, X-ray diffraction measurements, scanning electron microscopy and N 2 adsorption-desorption isotherms. The PTPA has a spherical-shape morphology and relatively high specific surface area. It is shown to be a viable adsorbent for solid phase extraction of 3-chlorophenol, 2,3-dichlorophenol, 2,4-dichlorophenol and 2,4.6-trichlorophenol prior to their determination by high performance liquid chromatography-mass spectrometry. Under the optimized conditions, recoveries from spiked samples are in the range from 92.5% to 106.3%. The limits of detection range from 0.03 to 0.3 ng mL −1 (at an S/N ratio of 3) in case of bottled juice, and from 0.03 to 0.1 ng g −1 in case of tomato samples. The enrichment factors for the four analytes are in the range of 127–183 for bottle juice, and from 110–150 for tomatos. Response is linear in the range of 1.0 to 40.0 ng mL −1 for juice, and 0.3–40.0 ng g −1 for tomatos. The relative standard deviations for the determination of the chlorophenols at 20 ng mL −1 in bottled beverage, and 20 ng g −1 in tomatos are lower than 5.7%.
A polytriphenylamine polymer (PTPA) was prepared by using an external cross-linker method with triphenylamine as monomer and dimethoxymethane as cross-linker, and it was used as an adsorbent to extract chlorophenols from juice and vegetable samples.
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JOURNAL OF MATERIALS SCIENCE
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2017
·Vol. 53
·期 7
·4861-4873
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IF 2.599
Ding Yang,Liu Jie,Zhu Yan,Nie Siyang,Wang Weili,Shi Junli,Miu Yanru,Yu Xibin
One-dimensional CaMoO 4 :RE 3+ (RE = Eu, Sm, Dy, Tb) nanofibers were successfully synthesized through a facile supersaturated recrystallization process. These CaMoO 4 :Ln 3+ nanofibers have the length ranging from 40 to 70 nm and the width of nearly 2 nm. Under UV excitation, these single-doped CaMoO 4 nanofibers shown stronger emission, and the photoluminescence quantum efficiency is 25.7% (CaMoO 4 :Eu 3+ ), 22.4% (CaMoO 4 :Sm 3+ ), 23.2% (CaMoO 4 :Dy 3+ ) and 27.7% (CaMoO 4 :Tb 3+ ), respectively. Moreover, our work reveals that various emission colors can be obtained and tuned from pure red to delight green via co-doping Tb 3+ and Eu 3+ in the CaMoO 4 host, and the energy transfer properties from Tb 3+ to Eu 3+ are also demonstrated. Finally, we apply the green CaMoO 4 :Tb 3+ , red CaMoO 4 :Eu 3+ and white CaMoO 4 :0.05Tb 3+ , 0.02Eu 3+ for LED devices, and these nanofiber-based LEDs present high efficiency and stability, which indicates the promising application in future optoelectronic fields.
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JOURNAL OF POROUS MATERIALS
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2018
·Vol. 26
·期 1
·7-17
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IF 1.858
Chen Mingsen,Qin Lanmei,Wei Yan,Liu Yuanli,Zhang Faai
Controlled/living free radical polymerization in confined space is expected to produce new materials with predetermined structures and special properties. In this work, various morphologies of mesoporous silicas were synthesized and used as a nanoreactor for bulk activators regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP) of styrene. The resulting mesoporous silica/polystyrene (MS/PS) nanocomposites and PS within the pore channels were characterized by scanning electron microscope, transmission electron microscope, nitrogen adsorption–desorption measurements, fourier transform infrared spectrometer, thermogravimetry analysis, gel permeation chromatography, differential scanning calorimetry, and proton nuclear magnetic resonance ( 1 H-NMR) spectra. In comparison to conventional bulk ARGET ATRP of styrene (without MS), the pore channels of the MS show an evident confinement effect on bulk ARGET ATRP of styrene. The morphology, molecular weight and polydispersity, glass transition temperature ( T g ), and spatial configuration of PS are strongly related to the morphology and internal pore channels of the MS. The bulk ARGET ATRP of styrene in 3D cubic bicontinuous structure MCM-48 shows a high potential for isospecific polymerization. The PS obtained in rod-like SBA-15 (2D), spherical SBA-15, and MCM-41 (1D) exhibit a higher molecular weight, polydispersity index and T g . The PS obtained in 3D MCM-48 exhibits enhanced molecular weight along with decreased the polydispersity. Moreover, the morphologies of the polymers obtained in mesoporous silica are consistent with that of the corresponding mesoporous silicas.
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CHROMATOGRAPHIA
·
2018
·Vol. 81
·期 4
·595-610
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IF 1.401
Liu Wei,Quan Ji
In this work, a quantitative structure–property relationship (QSPR) model based on atom-type electrotopological state (E-state) indices has been developed for predicting the dispersive liquid–liquid microextraction (DLLME) enrichment effect for aromatic organics by using 1-octyl-3-methylimidazolium hexafluorophosphate ([OMIM][PF 6 ]) ionic liquid as extractant. The enrichment factors of the aromatic organics obtained through DLLME-IL process were used as the dependent variable of the multiple linear regression (MLR) equation. The E-state indices of those organic compounds which combined together both electronic and topological characteristics of the analyzed molecules were used as molecular structure descriptors and the independent variable of the equation. The squared correlation coefficient R 2 for the MLR was 0.992 for the training set of 33 compounds. For the test set of 8 compounds, the linear regression coefficient was R 2 = 0.985. The results of this study showed that it can successfully predict enrichment factors of [OMIM][PF 6 ] in DLLME by using atom-type E-state indices, which can provide one more way for predicting the ability of ionic liquid to extract organic compounds based on their molecular structures.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2018
·Vol. 410
·期 15
·3587-3595
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IF 3.307
Chen Guilin,Wu Jianlin,Li Na,Guo Mingquan
Rhamnus davurica Pall. ( R. davurica ) has been used as a traditional medicine for many years in China and abroad and shown a wide spectrum of biological activities. Previously, we reported the phytochemical fingerprinting profile of R. davurica , its distinct anti-proliferative activities against HT-29 and SGC-7901 cell lines, and the topoisomerase I (Top I) ligands based on bio-affinity ultrafiltration and HPLC-MS (UF-HPLC-MS). Nevertheless, among the 32 peaks detected in the fingerprinting profile, the common bioactive constituents responsible for the anti-inflammatory and anti-proliferative activities in the extracts remain elusive. To further explore the specific responsible components for their diversified activities and their potential action targets/mechanisms, the method based on bio-affinity UF-HPLC-MS using therapeutic targets like Top I and cyclooxygenase 2 (COX-2) was established to rapidly screen and identify the ligands binding to these known target enzymes. As a result, 12 components were revealed as potential Top I ligands along with 11 components as potential COX-2 ligands, where several components were revealed to possess both activities. Further validations of these bioactive components have also been conducted and confirmed their highlighted activities. This integrated method of UF-HPLC-MS exhibits high efficiency in rapidly screening for multi-target bioactive components responsible for multiple pharmacological effects from the complex natural products and could be very useful to explain the complex action mechanisms of herb medicines in a complex multi-component and multi-target mode at the molecular level.
Schematic diagram of UF-HPLC-MS assay to screen for Top I and COX-2 ligands. The principle of the assay usually involves the following steps: incubation, ultrafiltration, and identification.
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International Journal of Peptide Research and Therapeutics
·
2017
·Vol. 25
·期 1
·161-168
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IF 0.904
Yu Zhiliang,Zhang Ling,Qin Wangrong,Yin Jianhua,Qiu Juanping
Polymyxin E (colistin) has been considered as an important peptide antibiotic for clinical treatment of Gram-negative pathogens by selectively targeting lipopolysaccharide on the outer membrane. Recent reports showed that colistin can also kill Gram-positive bacteria, but the mechanism of colistin against Gram-positive bacteria remains unclear. In this study, we found that colistin can kill its producer Paenibacillus polymyxa , a Gram-positive bacterium, by both disrupting its cell integrity and inducing oxidative stress. Enhancement of the cell permeability is the major action of colistin against P. polymyxa . In relative, colistin-induced oxidative stress plays an associated role in killing of P. polymyxa . Notably, we, for the first time, found that exogenous catalase is able to intensify the colistin-induced killing by inhibiting the intracellular catalase activity and thus increasing oxidative stress. Our findings would shed light on a not yet clearly described bactericidal mechanism of colistin against Gram-positive bacteria. More importantly, our findings pave the road for the future to lower clinical use of colistin for avoiding bacterial resistance.
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JOURNAL OF MATERIALS SCIENCE
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2018
·Vol. 53
·期 9
·6562-6573
·
IF 2.993
Jia Zhengfeng,Li Haoqi,Zhao Yao,Dikin Dmitriy A.,Ni Junjie,Zhao Limin,Zhen Jinming,Ge Bo,Shao Xin,Ren Fei
In this study, copper (Cu)/polydopamine (PDA) composite was fabricated by vacuum hot-press sintering of micrometer-sized Cu particles covered with PDA film tailored at the nanometer scale thickness. The resultant compacts exhibited much higher electrical conductivity than the uncoated counterparts. Analyses of sintered samples using scanning and transmission electron microscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy revealed the conversion of PDA into carbonized PDA (cPDA). The increased mass density and grain growth are likely responsible for improved electrical conductivity of the composite material.
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Biotechnology for Biofuels
·
2018
·Vol. 11
·期 1
·1-19
·
IF 5.497
Chen Yumeng,Shen Yaling,Wang Wei,Wei Dongzhi
Background The filamentous fungus Trichoderma reesei Rut-C30 is one of the most vital fungi for the production of cellulases, which can be used for biofuel production from lignocellulose. Nevertheless, the mechanism of transmission of external stimuli and signals in modulating cellulase production in T. reesei Rut-C30 remains unclear. Calcium is a known second messenger regulating cellulase gene expression in T. reesei.Results In this study, we found that a biologically relevant extracellular Mn2+ concentration markedly stimulates cellulase production, total protein secretion, and the intracellular Mn2+ concentration of Rut-C30, a cellulase hyper-producing strain of T. reesei. Furthermore, we identified two Mn2+ transport proteins, designated as TPHO84-1 and TPHO84-2, indicating that they are upstream in the signaling pathway that leads to cellulase upregulation. We also found that Mn2+ induced a significant increase in cytosolic Ca2+ concentration, and that this increased cytosolic Ca2+ might be a key step in the Mn2+-mediated regulation of cellulase gene transcription and production. The utilization of LaCl3 to block plasma membrane Ca2+ channels, and deletion of crz1 (calcineurin-responsive zinc finger transcription factor 1) to interrupt calcium signaling, showed that Mn2+ exerts the induction of cellulase genes via calcium channels and calcium signaling. To substantiate this, we identified a Ca2+/Mn2+ P-type ATPase, TPMR1, which could play a pivotal role in Ca2+/Mn2+ homeostasis and Mn2+ induction of cellulase genes in T. reesei Rut-C30.Conclusion sTaken together, our results revealed for the first time that Mn2+ stimulates cellulase production, and demonstrates that Mn2+ upregulates cellulase genes via calcium channels and calcium signaling. Our research also provides a direction to facilitate enhanced cellulase production by T. reesei.
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