JOURNAL OF SEPARATION SCIENCE
·
2016
·Vol. 40
·期 2
·466-471
·
IF 2.741
Shilei Ji,Nan Li,Li Qi,Minglin Wang
In this study, poly(styrene-co- N -methacryloyl- l -phenylalanine methyl ester)-functionalized magnetic nanoparticles were constructed and used as magnetic solid-phase extraction sorbents for analysis of food preservatives in beverages. To prepare the poly(amino acid)-based sorbents, N -methacryloyl- l -phenylalanine methyl ester, and styrene served as the functional monomers and modified onto the magnetic nanoparticles via free radical polymerization. Interestingly, compared with propylparaben and potassium sorbate, the proposed poly(amino acid)-based sorbents showed a good selectivity to sodium benzoate. The adsorption capacity of the sorbents to sodium benzoate was 6.08 ± 0.31 mg/g. Moreover, the fast adsorption equilibrium could be reached within 5 min. Further, the resultant poly(amino acid)-based sorbents were applied in the analysis of sodium benzoate in real beverage samples. The results proved that the proposed magnetic solid-phase extraction sorbents have a great potential for the analysis of preservatives in food samples.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Siqi Peng,Jing Wang,Mohamed A. Farag,Mahmoud Salah,Lianliang Liu,Yajing Fang,Weimin Zhang
This study investigated the changes in phytochemical composition and inflammatory response of crude papaya ( Carica papaya L.) seed oil (CPO) and its refined forms (degummed, PDG; deacidified, PDA; decolorized, PDC; deodorized, PDO). Oils were analyzed for their phytochemical composition, oil quality parameters, antioxidant activity , and their inflammatory response in LPS-stimulated THP-1 macrophages. At higher refining degrees, particularly after deacidification , the contents of phytochemicals (sterols, tocopherols , and polyphenols) decreased while oxidation products increased. Both CPO (0.1–1.0 mg/mL) and PDG reduced the secretion and mRNA expression of LPS-stimulated inflammatory cytokines and mediators and also blocked the activation of the NF-κB pathway. PDA, PDC, and PDO showed low anti-inflammatory or even pro-inflammatory activity. Correlation analysis showed that 4 polyphenols and 2 phytosterols were responsible for the oil's anti-inflammatory effects. These findings indicated that moderate refining is suggested for papaya seed oil processing for retaining bioactive ingredients and anti-inflammatory ability.
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Eco-Environment & Health
·
2025
·
IF 17.6
Lianzhou Shen,Yue Cai,Juan Gao
Nanoscale zero-valent iron loaded on biochar (nZVI@BC) has been proven to be effective in activating persulfate to remediate soil organic pollutants. However, studies on subsequent plant growth and microbial community changes in remediated soil remain limited. In this study, nZVI@BC, nZVI, and nanoscale biochar (nBC) were ball-mill produced and applied as amendments in pot experiments with PAH-contaminated soil to investigate their impacts on soil-crop (radish, Raphanus sativus L.) systems, and the widely distributed phenanthrene (Phe) was selected as model pollutant. The results indicate that nZVI@BC could induce more (75%) Phe accumulation in radish compared to the control treatment, but did not result in significant differences in plant biomass or enzyme activity. In Phe contaminated treatments, the Fe content of radish shoots increased from 86.87 ± 5.61 mg/kg DW without material application to 125.20 ± 11.93 mg/kg DW with nZVI@BC, while no significant differences were observed in roots. nZVI@BC and nBC increased the non-desorbed fraction of PAHs with low bio-availability by 13.6% and 10.2%, respectively, after 45 days compared to the control treatment. Illumina MiSeq sequencing revealed that nZVI@BC did not adversely affect the richness and diversity of soil microbial communities. Instead, it promoted the enrichment of bacteria related to the degradation of organic pollutants, such as Lysobacter and Spingomonas . The findings suggest that nZVI@BC after chemical oxidation remediation might be harmful to subsequent plants and ecosystems but much better than nZVI alone. The amount of nZVI@BC should be accurately calculated before chemical oxidation remediation.
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ENVIRONMENTAL POLLUTION
·
2025
·
IF 7.3
Chao Huang,Yilin Liu,Jing Lu,Xunzhi Deng,Zhoubin Liu,David P. Molloy,Langtao Xiao,Zhoufei Luo
Bisphenol A (BPA), an emerging endocrine-disrupting chemical found in irrigation water, soil, and agricultural plastic mulch, presents potential exposure risks to pepper plants ( Capsicum annuum L.). This study investigated BPA impact, observing that BPA accumulates in a concentration-dependent manner, with highest levels in roots and lowest in fruit. High BPA exposure decreased photosynthetic pigment content, reduced stomatal size, caused chloroplast dilation, and increased reactive oxygen species and malondialdehyde accumulation, disrupting hormone balance in leaves with a 2.3-fold increase in abscisic acid content under 100 mg/kg BPA treatment. Integrated multi-omics analyses revealed that BPA toxicity operates through interference with galactose metabolism, starch-sucrose metabolism, plant hormone signaling, and glutathione metabolism pathways. Key molecular responses included significant downregulation of photosynthesis-related genes and substantial metabolic perturbations, notably an 8.1-fold reduction in uridine diphosphate fructose and 10.6-fold accumulation of indole-3-ethanol. By employing an integrated multi-omics approach, this study provides a comprehensive mechanistic understanding of BPA phytotoxicity that extends beyond conventional physiological observations. The findings offer novel insights into the molecular regulatory networks underlying plant stress responses to endocrine-disrupting chemicals.
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Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Qing Guo,Min Zhang,Arun S. Mujumdar,Luming Rui
This work studied the effects of electric field (EF)-assisted treatment on the quality of arrowheads during freeze-thaw cycle (FTC), and the structural characteristics and physicochemical properties of arrowhead starch were emphatically investigated. The changes in color and hardness of the arrowheads indicated that EF-assisted treatment mitigated quality deterioration by reducing FTC-induced cold damage. For the structural and physicochemical properties of arrowhead starch, the size of starch granules, amylopectin/amylose ratio, and crystalline increased after FTC treatment, resulting in enhanced thermal stability and high resistant starch content. FTC treatment decreased molecular weight and weakened the hydrophilic interaction of arrowhead starch. EF-assisted treatment effectively minimized structural and physicochemical changes in arrowhead starch, which was in favor maintain their initial status. This study demonstrated that EF-assisted treatment has positive effects on maintaining arrowhead starch structures and arrowhead quality during FTC. Industrial relevance Electric field as a novel processing technology is promising in maintaining the quality of fresh foods during freezing and thawing. This work indicated that electric field-assisted treatment greatly reduced the mechanical damage of ice crystal on arrowhead and inhibited the structural variations of arrowhead starch, providing a good strategy for the preservation of agricultural products.
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Regenerative Biomaterials
·
2021
·Vol. 8
·期 5
·
IF 6.353
Liu Boxun,Jin Zhizhong,Chen Haiyan,Liang Lun,Li Yao,Wang Guo,Zhang Jing,Xu Tao
Electrospun membranes are attracting interest as a drug delivery system because of their material composition flexibility and versatile drug loading. In this study, the electrospun membrane was loaded with doxorubicin (DOX) via electrostatic adsorption for long-term drug delivery. DOX loading process was optimized by varying temperature, time, drug concentration, pH and ionic strength of solutions. The loading process did not impair the structural properties of the membrane. Next, we investigated the drug release kinetics using spectroscopic techniques. The composite membranes released 22% of the adsorbed DOX over the first 48 h, followed by a slower and sustained release over 4 weeks. The DOX release was sensitive to acidic solutions that the release rate at pH 6.0 was 1.27 times as that at pH 7.4. The DOX-loaded membranes were found to be cytotoxic to U-87 MG cells in vitro that decreased the cell viability from 82.92% to 25.49% from 24 to 72 h of co-incubation. These membranes showed strong efficacy in suppressing tumour growth in vivo in glioblastoma-bearing mice that decreased the tumour volume by 77.33% compared with blank membrane-treated group on Day 20. In conclusion, we have developed an effective approach to load DOX within a clinically approved poly (L-lactic acid)/gelatine membrane for local and long-term delivery of DOX for the treatment of glioblastoma.
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INORGANICA CHIMICA ACTA
·
2017
·
IF 2.002
Nan Zhang,Yuhua Fan,Guimei Huang,Daniela Buac,Caifeng Bi,Yanfei Ma,Xinping Wang,Zhen Zhang,Xia Zhang,Q. Ping Dou
Although cadmium is a widespread environmental contaminant and human carcinogen, our studies indicate an organic cadmium complex to be a potent inhibitor of proteasomal chymotrypsin-like (CT-like) activity, further capable of inducing apoptosis in a cancer cell-specific manner. Many clinical studies suggest the use of proteasome inhibitors as potential novel anticancer agents and in the present study, we have synthesized three novel l -tryptophan-containing cadmium complexes: Cd(C 17 H 15 N 4 O 2 ) 2 ·2CH 3 OH ( 1 ) (C 17 H 15 N 4 O 2 = 2-acetylpyrazine- l -tryptophan), Cd(C 17 H 15 N 2 O 3 ) 2 ·2CH 3 OH ( 2 ) (C 17 H 15 N 2 O 3 = 5-methylfurfural- l -tryptophan) and Cd(C 16 H 12 N 2 O 2 SBr) 2 ·2CH 3 OH ( 3 ) (C 16 H 12 N 2 O 2 SBr = 5-bromo-2-thiophenecarbaldehyde- l -tryptophan) and found that under comparable conditions cadmium complexes 1 and 2 , but not 3 , have proteasome-inhibitory activity in human breast cancer MDA-MB-231 cells. Our results suggest that l -tryptophan Schiff base cadmium complexes are potent proteasome inhibitors.
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RSC Advances
·
2017
·Vol. 7
·期 52
·32786-32794
·
IF 3.108
Jingqian Fan,Yuangang Liu,Shibin Wang,Yulu Liu,Siming Li,Ruimin Long,Ran Zhang,Ranjith Kumar Kankala
Layer-by-Layer (LbL) self-assembly of nanocarriers has garnered the interest of researchers for a wide variety of biomedical applications. In this study, we demonstrated the preparation of poly(lactide-co-glycolide) (PLGA)-(poly-L-ornithine (PLO)/fucoidan)4 core–shell nanoparticles (LbL NPs) by a LbL-based self-assembly process, which possessed a mean size of 170 nm. In LbL NPs, a drug carrying PLGA nano-core is coated with alternating PLO and sulfated polysaccharide fucoidan composite films as a shell on the surface. The anti-tumor drug doxorubicin (DOX) loaded into the PLGA core, resulted in better encapsulation efficiency and its in vitro release from LbL NPs demonstrates that this core–shell strategy takes an advantage of its ability to hold the drug cargo and exhibit controlled release. Further, in vitro cell uptake studies by confocal laser scanning microscopy (CLSM) examination in breast tumor cells (MCF-7 cell line) have confirmed that the nanocarriers are successfully internalized and outlined their presence in the cytoplasm after 4 h of incubation. These intracellularly delivered DOX-loaded LbL NPs exhibited significant anti-tumor activity against breast tumor cells. This innovative chemotherapeutic design taking above advantages of successful internalization along with controlled release property signifies as a promising interventional therapeutic delivery system.
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3 Biotech
·
2021
·Vol. 11
·期 6
·1-14
·
IF 2.406
Appu Manikandan,Lian Zhifeng,Zhao Dengqi,Huang Jianying
In this study, we prepared chitosan (CS)-coated iron oxide (Fe 3 O 4 ) nanocomposites (NCs) by employing the aqueous leaf extract of Brassica oleracea L. and evaluated its antimicrobial potential. The characterization of hybrid CS-Fe 3 O 4 NCs was performed using Fourier-transform infrared spectroscopy (FTIR) analysis to evaluate the chemical bonding of chitosan to nanoparticles (NPs). X-ray photoelectron spectroscopy (XPS) studies revealed the presence of oxidation state elements Fe 2p, O 1s, N 1s, and C 1s, and the zeta potential analysis was found to have well-colloidal stability (+ 76.9 mV) of NCs. Transmission electron microscopy (TEM) analysis determined that CS-Fe 3 O 4 NCs were spherical with an average particle size of 27 nm. The X-ray diffractometer (XRD) spectrum ascertained the crystallinity of the hybrid NCs and the vibrating sample magnetometer (VSM) inferred the ferromagnetic behavior of the synthesized NCs. Furthermore, the significant antibacterial efficacy of NPs was demonstrated against foodborne bacterial pathogens, such as Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ), and the highest zone of inhibition was observed to be 11.5 mm and 13.5 mm in CS-Fe 3 O 4 NCs, respectively. In comparison with Fe 3 O 4 NPs, synergistic impacts of CS-Fe 3 O 4 NCs displayed great antibacterial potential as exhibited by a clearly enlarged zone. Thus, CS-Fe 3 O 4 NCs could be used as efficacious antimicrobial agents in food packaging and food preservation fields.
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Foods
·
2022
·Vol. 11
·期 22
·3731
·
IF 5.561
Ziying Hu,Yanan Ma,Jun Liu,Yijun Fan,Anran Zheng,Pengyan Gao,Liang Wang,Dunhua Liu
Goji berry (Lycium barbarumL., LBL) is a good source of carotenoids, while the bioaccessibility of various types of LBL carotenoids has not been explored. In the study, eight carotenoids, three carotenoid esters and two carotenoid glycosylated derivatives were identified by a non−targeted metabolomics approach. The dried LBL (DRI), LBL in water (WAT), and LBL in “Baijiu” (WIN) were used to recreate the three regularly chosen types of utilization, and the in vitro digestion model showed that the bioaccessibility of the carotenoids increased significantly from the oral to the gastric and intestinal phase (p< 0.05). The bioaccessibility of LBL carotenoids was the most elevated for DRI (at 28.2%), followed by WIN and WAT (at 24.9% and 20.3%, respectively). Among the three carotenoids, zeaxanthin dipalmitate showed the highest bioaccessibility (51.8–57.1%), followed by β−carotene (51.1–55.6%) and zeaxanthin (45.2–56.3%). However, the zeaxanthin from DRI exhibited significantly higher bioaccessibility (up to 58.3%) than WAT and WIN in both the gastric and intestinal phases (p< 0.05). Results of antioxidant activity tests based on DPPH, FRAP, and ABTS showed that the addition of lipids improved the bioaccessibility of the carotenoids. (p< 0.05).Keywords:goji berry;bioaccessibility;carotenoids;metabolomics;zeaxanthin dipalmita
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Journal of Analytical Methods in Chemistry
·
2022
·
IF 2.594
Shihan Qin,Mingjuan Liu,Sunv Tang,E. Shuai,Ziming Wang,Kaiquan Yu,Wei Cai
Diospyros lotus L, F. Ebenaceae, is an edible fruit that is widely distributed in China and other Asian countries. Presently, Diospyros lotus L can be used to treat patients with diabetes; however, its chemical composition and pharmacological profiles remain to be elucidated. This study investigated the potential bioactive compounds of Diospyros lotus L and their mechanisms of action using LC-MS and network pharmacology analysis. First, the components of Diospyros lotus L were identify using a reliable strategy for UHPLC-Q-Exactive Orbitrap mass spectrometry combined with parallel reaction monitoring (PRM) in the negative ion mode. Second, a network pharmacology study, including target gene prediction and functional enrichment, was applied to screen the main quality markers of Diospyros lotus L and explore its potential mechanism for the treatment of diabetes. The results showed that a total of 159 compounds were identified from Diospyros lotus L, among which, 140 were reported for the first time. Furthermore, 40 active components, such as quercetin, luteolin, and kaempferol, were proposed as active components of Diospyros lotus L for the treatment of diabetes based on network pharmacology analysis. In addition, 92 relevant antidiabetic targets were mainly related to positive regulation of transcription from the RNA polymerase II promoter, extracellular space, and protein binding, suggesting the involvement of TNF, PI3K-Akt, and HIF-1 signaling pathways in the antidiabetic effect of Diospyros lotus L. Our results may provide a useful approach to identify potential active components and molecular mechanisms of Diospyros lotus L for the treatment of diabetes.
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ENVIRONMENTAL POLLUTION
·
2020
·
IF 6.793
Yuankun Liu,Min Lu,Qi Tao,Jipeng Luo,Jinxing Li,Xinyu Guo,Yongchao Liang,Xiaoe Yang,Tingqiang Li
The radial transport of cadmium (Cd) is essential for Cd influx in roots. The role of radial transport pathway on the Cd translocation from root to shoot among wheat genotypes are still poorly understood. This study explored the role of apoplastic and symplastic pathway on root Cd uptake and root-to-shoot translocation in Zhenmai 10 (ZM10, high Cd in grains) and Aikang 58 (AK58, low Cd in grains). Under Cd treatment, the deposition of Casparian strips (CSs) and suberin lamellae (SL) initiated closer to the root apex in ZM10 than that in AK58, which resulted in the lower Cd concentration in apoplastic fluid of ZM10. Simultaneously, Cd-induced expression levels of genes related to Cd uptake in roots were significantly higher in AK58 by contrast with ZM10, contributing to the symplastic Cd accumulation in AK58 root. Moreover, the addition of metabolic inhibitor CCCP noticeably decreased the Cd accumulation in root of both genotypes. Intriguingly, compared to ZM10, greater amounts of Cd were sequestrated in the cell walls and vacuoles in roots of AK58, limiting the translocation of Cd from root to shoot. Furthermore, the elevated TaHMA2 expression in ZM10 indicates that ZM10 had a higher capacity of xylem loading Cd than AK58. All of these results herein suggest that the radial transport is significant for Cd accumulation in roots, but it cannot explain the difference in root-to-shoot translocation of Cd in wheat genotypes with contrast Cd accumulation in grains.
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