JOURNAL OF CHEMICAL THERMODYNAMICS
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2019
·
IF 2.29
Kun Fu,Pan Zhang,Dong Fu
The absorption amount ( m ) and loading ( α ) of CO 2 in 2-(ethylamino)ethanol (EAE) activated 3-(dimethylamino)propan-1-ol (3DMA1P) aqueous solution were measured. The temperatures ranged from 303.2 K to 323.2 K. The mass fractions of 3DMA1P and EAE respectively ranged from 0.3 to 0.5 and 0.01 to 0.03. The effects of temperature ( T ) and mass fractions of amines ( w ) on the absorption performance were illustrated. It’s impressive that the addition of very small amounts of EAE into 3DMA1P aqueous solution significantly enhanced the absorption performance. Moreover, comparison showed that 3DMA1P-EAE aqueous solution appeared much higher CO 2 absorption capacity than the widely used MDEA-MEA aqueous solution. Tray towers with different plates ( N p = 6, 8 and 10) were used to verify the removal efficiency of CO 2 ( η CO 2 ) and the effects of gas flow rate ( G ), absorbent flow rate ( L ), w , absorbent feed temperature ( T ) and N p on η CO 2 were illustrated. Our results showed that when composed of 30% to 50% (mass percent) 3DMA1P and 3% EAE, the absorbents achieved η CO 2 higher than 90%, which met the efficiency requirement specified in a power plant, thus leading to a promising industrial application in the CO 2 capture process.
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JOURNAL OF MOLECULAR LIQUIDS
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2017
·
IF 3.648
Yaran Yin,Taotao Fu,Chunying Zhu,Youguang Ma
Densities and viscosities of binary mixtures {[Bmim][BF 4 ] + MDEA}, {MDEA + H 2 O} and ternary mixture {[Bmim][BF 4 ] + MDEA + H 2 O} were measured at T = (293.15–333.15) K. The volumetric and viscometric properties of binary and ternary mixtures, such as excess molar volume ( V E ), viscosity deviation (Δ η ), excess Gibbs energy of activation of viscous flow (Δ G ⁎E ), and Grunberg-Nissan interaction parameter ( G 12 ), were deduced from experimental data. The excess molar volumes and viscosities data were used to calculate the Redlich-Kister and Jouyban-Acree equations coefficients for binary mixtures , and Singh et al. and Jouyban-Acree extended equations coefficients for ternary mixtures. The intermolecular interactions in binary and ternary mixtures were discussed through volumetric and viscometric properties and FT-IR spectroscopic analysis .
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-10
·
IF 4.259
Chu Qiang,Zhao Yiying,Shi Xuer,Han Wen,Zhang Yanzhen,Zheng Xiaodong,Zhu Jing
To address the need for a high throughput toxicity test in the modern food industry, an in vivo -like 3-D cell model was constructed in this study to provide an alternative to controversial long-term animal models and to improve the sensitivity and accuracy of the traditional monolayer model. The model formed cell cylindroids within polyvinylidene fluoride (PVDF) hollow fibers and therefore mimicked the microenvironment of liver tissue. Microscopy methods were used, and liver-specific functions were measured to demonstrate the superiority of the model compared to the monolayer model, as well as to optimize the model for best cell performances. Later, toxicity tests of sodium nitrite and acrylamide were conducted in both the 3-D model and the monolayer model to study the sensitivity of the 3-D model in toxicity responses. As expected, HepG2 cells within the 3-D model responded at lower concentrations and shorter exposure times compared to cells within the monolayer model. Furthermore, western blot analysis of apoptosis pathways also supported the argument.
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Experimental and Therapeutic Medicine
·
2017
·Vol. 14
·期 5
·4307-4311
·
IF 1.261
Tao Zhou,Shanliang Guo,Shaolin Wang,Qiong Li,Mingsheng Zhang
This study was designed to investigate possible protective effects of sevoflurane on myocardial ischemia-reperfusion injury (MIRI) and its impact on expression of HIF-1α and caspase-3 in rats, so as to provide new insights for the treatment of MIRI. Forty SD rats were randomly divided into four groups (n=10) including Sham operation (Sham), ischemia-reperfusion (IR), sevoflurane preconditioning group (Sevo-Pre) and sevoflurane post-conditioning (Sevo-Post) groups. Perfusion was performed using ex vivo heart perfusion. The baseline values of cardiac function were recorded in each group at the end of balanced perfusion and after 60 min of reperfusion. Myocardial infarct size (MIS) was calculated at the end of perfusion using TTC staining. Levels of HIF-1α and caspase-3 protein and HIF-1α (western blotting) and Bcl-2 mRNA (RT-qPCR) were detected at the end of reperfusion. Our results showed no significant differences in cardiac function between the groups at the end of the balanced perfusion. After reperfusion for 60 min, however, the cardiac functions of the Sevo-Pre and Sevo-Post groups were significantly better than those in the IR group, and the MIS at the end of reperfusion was significantly decreased. Western blotting and RT-qPCR showed that expression of HIF-1α protein was significantly increased, expression of caspase-3 protein was significantly decreased and expression of HIF-1α and Bcl-2 mRNA were significantly increased in Sevo-Pre and Sevo‑Post groups compared with the levels in the IR group at the end of reperfusion. There were no significant differences in experimental results between Sevo-Pre and Sevo-Post groups. Our data support the idea that sevoflurane can improve MIRI in rats by improving cardiac function and reducing MIS. This protective effect seems to be achieved by activation of HIF-1α and inhibition of caspase-3.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2024
·
IF 2.5
He Liu,Qian Gao,Kaiyue Zhang,Meng Xu,Hualei Wang,Dongzhi Wei
( R )-selective transaminases have the potential to act as efficient biocatalysts for the synthesis of important pharmaceutical intermediates. However, their low catalytic efficiency and unfavorable equilibrium limit their industrial application. Seven ( R )-selective transaminases were identified using homologous sequence mining. Beginning with the optimal candidate from Mycolicibacterium hippocampi , virtual mutagenesis and substrate tunnel engineering were performed to improve catalytic efficiency. The obtained variant, T282S/Q137E, exhibited 3.68-fold greater catalytic efficiency ( k cat / K m ) than the wild-type enzyme . Using substrate fed-batch and air sweeping processes, effective conversion of 100 mM 4-hydroxy-2-butanone was achieved with a conversion rate of 93 % and an ee value > 99.9 %. This study provides a basis for mutation of ( R )-selective transaminases and offers an efficient biocatalytic process for the asymmetric synthesis of ( R )-3-aminobutanol.
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JOURNAL OF POWER SOURCES
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2024
·
IF 8.1
Yang Chen,Jianda Niu,Siheng Zhang,Zhixian Dong,Jinbao Xu,Caihong Lei
The quest for next-generation energy storage systems has led to the development of high-voltage all-solid-state lithium metal batteries (ASSLMBs), which promise unprecedented energy density and safety. However, the challenge lies in creating of a robust electrolyte that can meet high room temperature ionic conductivity, Li-ion transference number and a wide electrochemical stability window (ESW) while maintaining flexibility and compatibility with lithium metal anodes. Here, novel polyurethane-based composite polymer electrolytes (CPEs) have been successfully fabricated by the cross-linking reaction of poly(1,5-dioxepan-2-one) diols (poly(ether-ester) soft segment) and hexamethylene diisocyanate trimer (hard segment) (PDH) blending with inorganic NASICON-type ceramic fillers Li 1.3 Al 0.3 Ti 1.7 (PO 4 ) 3 (PDH@LATP). The resulting CPE exhibits an impressive ionic conductivity of 1.65 × 10 −4 S cm −1 at room temperature and a Li-ion transference number of 0.63, which is attributed to the amorphous structure of PDH and the formation of an interconnected ionic pathway by the inorganic filler. The incorporation of LATP also improve the ESW of CPE to 5.3 V while maintaining good mechanical properties. Therefore, the assembled LiFePO 4 /PDH@LATP/Li batteries displayed low interfacial resistance, Li dendrite suppression, high specific capacity and excellent cyclic performance. The high-voltage LiNi 0.5 Co 0.2 Mn 0.3 O 2 /PDH@LATP/Li batteries also exhibits good cycling performance and durability. The design principles and materials presented herein provide a blueprint for the next wave of ASSLMBs.
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Chinese Medicine
·
2024
·Vol. 19
·期 1
·1-17
·
IF 5.3
Gao Jiaming,Guo Hao,Li Junmei,Zhan Min,You Yue,Xin Gaojie,Liu Zixin,Fan Xiaodi,Gao Qinghe,Liu Jianxun,Zhang Yehao,Fu Jianhua
Background Buyang Huanwu Decoction (BYHWD) is a traditional Chinese medicine to treat the syndrome of qi deficiency and blood stasis. Platelets play an important role in regulating thrombus and inflammation after ischemic injury, studies have shown that BYHWD regulate myocardial fibrosis and exert anti-inflammatory effects through IL-17 and TLR4 pathways, but the mechanism of platelet activation by BYHWD in stable coronary heart disease is still unknown. In the present study, model of left anterior descending coronary artery ligation was applied to investigate the mechanisms of BYHWD on modulating platelets hyperreactivity and heart function after fibrosis of ischemic myocardial infarction (MI). Methods Myocardial infarction model was constructed by ligation of the left anterior descending coronary artery. The rats were randomly divided into five groups: sham, model, MI with aspirin (positive), MI with a low dosage of BYHWD (BYHWD-ld) and MI with a high dosage of BYHWD (BYHWD-hd) for 28 days. Results Coronary artery ligation prominently induced left ventricle dysfunction, increased cardiomyocyte fibrosis, which was accompanied by platelets with hyperreactivity, and high levels of inflammatory factors. BYHWD obviously reversed cardiac dysfunction and fibrosis, increased the thickness of the left ventricular wall, and inhibited aggregation ratio and CD62p expression. BYHWD restored the mitochondrial respiration of platelets after MI, concomitant with an increased telomere expression and decreased inflammation. According to the result of transcriptome sequencing, we found that 106 differentially expressed genes compared model with BYHWD treatment. Enrichment analysis screened out the Ras-related protein Rap-1 (Rap1) signaling pathway and platelet activation biological function. Quantitative real-time PCR and Western blotting were applied to found that BYHWD reduced the expression of Rap1/PI3K-Akt/Src-CDC42 genes and attenuated the overactivity of PI3 kinase/Rap1/integrin α(IIb)β(3) pathway. Conclusion BYHWD reduced inflammation and platelet activation via the PI3 kinase/Rap1/integrin α(IIb)β(3) pathway and improved heart function after MI. Graphical Abstract
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ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Chaoqiang Wu,Xin Zhang,Wei Liu,Chengyong Wang,Qianqian Jiang,Feifei Chen,Quanzhen Liu,Fei Cao,Gaowei Zheng,Alei Zhang,Kequan Chen
Chitin, as the second most abundant biomass in nature, possesses 7 wt % biologically fixed nitrogen, and its direct valorization into valuable nitrogen (N)-containing chemicals is optimal. Herein, we report the biosynthesis of two specific furan-based amine compounds, 2-acetyl-4-aminofuran (2A4AF) and 3-acetylamino-5-(α-aminoethyl)-furan (3A5AEF), from chitin-derived 3-acetamido-5-acetylfuran (3A5AF) via biocatalytic deacetylation and reduction amination, respectively. An amidase (MmH) from Starkeya sp. and an R-selective aminotransferase (ATA117) from Arthrobacter sp. were obtained, which can, respectively, convert 3A5AF to 2A4AF with a yield of 79.3% (47.58 mM) and (R)-3A5AEF (enantiomeric excess (ee) value of >99%) with a yield of 84.0% (126 mM), respectively, under the optimal conditions. The biosynthesis efficiency of the two amines was high in comparison with chemical approaches. This work paves the way for green and efficient production of furan-based amino chemicals from inexpensive renewable chitin resources.
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POLYMER
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2012
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IF 3.438
Changwei Huang,Nianfa Yang,Anlin Zhang,Liwen Yang
Optically active helical poly[( R or S )-3-(9-alkylfluoren-9-yl)propene oxide]s (poly-AFPOs) were used to induce the enantioselective addition of methyllithium to aldehydes. The addition of methyllithium to 4-methoxybenzaldehyde gave the highest enantioselectivity (88% ee) under the inducement of poly-AFPOs. Poly-AFPOs could be recovered simply by pouring the reaction mixture into methanol in the end of the reaction and separating the precipitated polymer via filtration. The recovered poly-AFPOs could be reused for many times to induce the enantioselective addition of methyllithium to aldehyde without losing their enantioselective inducement ability. The investigation of the optical rotation and the MALDI-ToF MS confirmed that, in the enantioselective addition induced by poly-AFPOs, the lithium cation was embedded in the hole of the helical column of poly-AFPOs, leaving the negative methyl ion outside the one-handed helical column. The methyl anion abutted tightly against the helical polymer and acquired a chiral environment. It was this helically chiral environment that resulted in the enantioselective inducement of the addition reaction.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2011
·Vol. 13
·期 32
·14542-14549
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IF 3.454
Kelei Zhuo,Yujuan Chen,Jing Chen,Guangyue Bai,Jianji Wang
Extensive applications of ionic liquids (ILs) may result in their accumulation in the ecological environment and organisms. Although ILs are popularly called “green solvents”, their toxicity, in fact, has been exhibited. Therefore the interaction of ILs with biomolecules is a cutting-edge research subject. Herein, the interactions of 1-butyl-3-methylimidazolium carboxylate ionic liquids ([C4mim][HCOO], [C4mim][CH3COO] and [C4mim][CH3CH2COO]) with glucose in water were studied for their volumetric properties, viscosity, conductivity and NMR spectra. Limiting apparent molar volumes (V0Φ,IL), viscosity B-coefficients, limiting molar conductivities (Λ0) and Walden products (Λ0η0) were evaluated for the ILs in glucose + water solutions. Volumetric interaction parameters were also obtained from the transfer volumes of the ionic liquids. The contributions of the solvent properties (B1) and the ionic liquid–solvent interactions (B2) to the B-coefficient were extracted, together with molar activation energies (Δμ0≠IL) of the ionic liquids for viscous flow of the aqueous glucose + IL solution. In addition, the 13C and 1H NMR spectra of methyl β-D-glucopyranoside and ILs in β-D-glucopyranoside + IL + D2O were studied. The NMR results show that no special and strong interactions were observed between glucopyranoside and the ILs. However, it was confirmed that the H2 on the imidazolium ring has more activity (acidity) than atoms H4 and H5. The macro-properties and their changes were also discussed in terms of the size, structure and solvation of the ILs and glucose.
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JOURNAL OF SEPARATION SCIENCE
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2016
·Vol. 39
·期 23
·4585-4591
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IF 2.741
Xinpei Li,Shigang Wei,Ying Sun,Qun Sun,Li Liang,Bo Zhang,Huilan Piao,Daqian Song,Xinghua Wang
A novel and simple supported ionic-liquid-based solid-phase extraction method for the determination of triazine herbicides in rice was developed. Glass slides were functionalized by an ionic liquid, 1-carboxyethyl-3-methylimidazolium chloride, and were used for the simultaneous extraction of seven triazine herbicides in rice samples. The effects of the type of extraction solvent, the extraction time, the type and volume of loading solvent, and the type of eluting solvent on the extraction efficiency were investigated and optimized. Under the optimum operation conditions, the limits of detection for seven triazine herbicides in rice samples obtained by high-performance liquid chromatography were 3.16–5.42 ng/g, which were lower than the maximum residue levels established by various organizations. The linear correlation coefficients were higher than 0.9975 in the concentration range of 0.015–1.08 μg/g for the seven triazine herbicides. The recoveries of the seven triazine herbicides at the two concentration levels of 0.15 and 0.45 μg/g are between 82.47 and 104.21%, with relative standard deviations of 0.69–9.19%. The intra- and inter-day ( n = 5) precisions for all triazine herbicides at the spiked level of 0.30 μg/g were 1.72–11.71%.
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BIOCHIMIE
·
2016
·
IF 3.017
Rong-zu Nie,Wei Zhu,Jin-ming Peng,Zhen-zhen Ge,Chun-mei Li
Because fibrillary protein aggregates is regarded to be closely associated with many diseases such as Alzheimer's disease, diabetes, and Parkinson's disease, growing interest and researches have been focused on finding potential fibrillation inhibitors. In the present study, the inhibitory effects of epigallocatechin-3-gallate (EGCG) and A-type dimeric epigallocatechin-3-gallate (A-type EGCG dimer) on the formation of insulin fibrillation were compared by multi-dimensional approaches including thioflavin-T (ThT) fluorescence assay , 1-anilinonaphthalene-8-sulfonic (ANS) fluorescence assay, dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy and circular dichroism (CD) spectroscopy. Our results confirmed that A-type EGCG dimer is a more potent inhibitor against the formation of bovine insulin amyloid fibril than EGCG. In addition, A-type EGCG dimer could not only inhibit insulin amyloid fibril formation, but also change the aggregation pathway and induce bovine insulin into amorphous aggregates. The results of the present study may provide a new guide on finding novel anti-amyloidogenic agents.
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