JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2015
·
IF 2.912
Hua Sun,Xiaotong Zhou,Xingwang Zhang,Baojian Wu
In this study, the role of futile recycling (or deglucuronidation) in the disposition of two flavonoids (i.e., genistein and apigenin) was explored using UGT1A1-overexpressing HeLa cells (or HeLa1A1 cells). Glucuronidation of the flavonoids by HeLa1A1 cell lysate followed the substrate inhibition kinetics (Vmax = 0.10 nmol/min/mg, Km = 0.54 μM, and Ksi = 2.0 μM for genistein; Vmax = 0.19 nmol/min/mg, Km = 0.56 μM, and Ksi = 3.7 μM for apigenin). Glucuronide was efficiently generated and excreted after incubation of the cells with the aglycone (at doses of 1.25–20 nmol). The excretion rates were 0.40–0.69 and 0.84–1.1 nmol/min/mg protein for genistein glucuronide (GG) and apigenin glucuronide (AG), respectively. Furthermore, glucuronide excretion and total glucuronidation were significantly reduced in MRP4 knocked-down as compared to control cells. The alterations were well characterized by a two-compartment pharmacokinetic model incorporating the process of futile recycling (defined by a first-order rate constant, Kde). The derived Kde values were 15 and 25 h–1 for GG and AG, respectively. This was well consistent with the in vitro observation that AG was subjected to more efficient futile recycling compared to GG. In conclusion, futile recycling was involved in cellular glucuronidation, accounting for transporter-dependent glucuronidation of flavonoids.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF INORGANIC BIOCHEMISTRY
·
2015
·
IF 3.444
Lan-mei Chen,Fa Peng,Guo-dong Li,Xin-ming Jie,Kang-rong Cai,Chun Cai,Yu Zhong,Hua Zeng,Wu Li,Zhen Zhang,Jin-can Chen
A new ruthenium methylimidazole complex [Ru(MeIm) 4 ( p -cpip)] 2 + ( Ru1 , p -cpip = 2-(4-chlorophenyl)-1 H-imidazo[4,5-f][1,10]phenanthroline, MeIm = 1-methylimidazole) has been synthesized and characterized. The cellular uptake, in vitro cytotoxicities, cell cycle arrest and apoptosis-inducing mechanism of this Ru(II) complex have been extensively explored by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry , Comet assay , inverted fluorescence microscope as well as Western blotting experimental techniques. Notably, Ru1 displayed relatively high cytotoxic activity against lung cancer A549 cells and had high selectivity between tumor and normal cells in comparison with cisplatin . Further studies showed that Ru1 caused cell cycle arrest at G0/G1 phase and induced apoptosis via the mitochondrial pathway, which involved reactive oxygen species (ROS) accumulation, mitochondrial dysfunction and Bcl-2 and caspase correlative family member activation. For providing more information about the possible antitumor mechanism, the in vitro DNA binding studies have been also investigated by different spectrophotometric methods, thermal denaturation and viscosity measurements.
✓ 已验证
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2017
·
IF 9.446
Ruowen Liang,Fenfen Jing,Guiyang Yan,Ling Wu
Visible-light-initiated organic transformations have received much attention because of low cost, relative safety, and environmental friendliness. In this work, a series of CdS-decorated MIL-68(Fe) nanocomposites (CdS-M68 NCs) have been prepared via a facile room-temperature photodeposition technique in a controlled manner. Importantly, the CdS-M68 NCs exhibit remarkably enhanced photoactivity toward selective reduction of 4-nitroaniline (4-NA) to p -phenylenediamine (PPD) in water under visible light irradiation (λ ≥ 420 nm) as compared to bare CdS and MIL-68(Fe), giving a 4-NA conversion of ∼100% after irradiation for 8 min. The significantly enhanced photoactivity is attributed to the integrated factors of the effective transportation of the photogenerated electron-hole pairs and the enhanced visible light absorption intensity. Combining with trapping experiments and ESR analysis, it could be revealed that the photoexcited electrons and CO 2− radicals should be the main active species in the present system. In addition, a possible photocatalytic reduction mechanism has been investigated.
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6
Yong-Jun Huang,Yi-Peng Zang,Li-Jun Peng,Ming-Han Yang,Jing Lin,Wei-Min Chen
The low permeability of the outer membrane of Gram-negative bacteria is a serious obstacle to the development of new antibiotics against them. Conjugation of antibiotic with siderophore based on the “Trojan horse strategy” is a promising strategy to overcome the outer membrane obstacle. In this study, series of antibacterial agents were designed and synthesized by conjugating the 3-hydroxypyridin-4(1 H )-one based siderophores with cajaninstilbene acid ( CSA ) derivative 4 which shows good activity against Gram-positive bacteria by targeting their cell membranes but is ineffective against Gram-negative bacteria. Compared to the inactive parent compound 4 , the conjugates 45c or 45d exhibits significant improvement in activity against Gram-negative bacteria, including Escherichia coli , Klebsiella pneumoniae and especially P. aeruginosa (minimum inhibitory concentrations, MICs = 7.8–31.25 μM). The antibacterial activity of the conjugates is attributed to the CSA derivative moiety, and the action mechanism is by disruption of bacterial cell membranes. Further studies on the uptake mechanisms showed that the bacterial siderophore-dependent iron transport system was involved in the uptake of the conjugates. In addition, the conjugates 45c and 45d showed a lower cytotoxic effects in vivo and in vitro and a positive therapeutic effect in the treatment of C. elegans infected by P. aeruginosa . Overall, our work describes a new class and a promising 3-hydroxypyridin-4(1 H )-one- CSA derivative conjugates for further development as antibacterial agents against Gram-negative bacteria.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ming Ouyang,Xi Yu,Jiani Zhong,Yan Mao,Yunqian Cui,Huiqin Jiang,Xiaoying Lu,Weiyu Cao,Zeting Yuan,Jingbo Yin
The inflammatory environment of periodontitis with bacteria, excessive reactive oxygen species (ROS) and limited regenerative capacity of alveolar bone makes reconstruction of periodontium become a huge challenge. The present strategies, such as local debridement and antibiotic injection, are difficult to solve above problems completely. Thus, to reverse the progression of the disease, Ca 2+ -tannic acid nanocomposites loaded into injectable sodium alginate/4-arm polyethylene glycol-lipoic acid hydrogel (CaTA@Gel) were fabricated, including Ca 2+ ‑sodium alginate (SA) ionic crosslinking and radical polymerization of lipoic acid-modified 4-arm polyethylene glycol (PEG-SS 4 ) under UV illumination. In our results, the double networks of hydrogel not only had good mechanical property, but also sustainedly released CaTA nanocomposites which could inhibit bacteria, scavenge ROS due to TA, and promote osteogenesis of bone mesenchymal stem cells (BMSCs) via Ca 2+ . The antibacterial rates of CaTA (1 mg/mL) against E. coli and S. aureus were 97.3 ± 1.25 % and 96.7 ± 2.57 %, respectively. Meanwhile, ·OH and ·O 2− could be inhibited obviously by CaTA (100 μg/mL), whose inhibition rate were 59.6 ± 2.98 % and 62.4 ± 3.12 %, respectively. Finally, the up-regulation of osteogenetic genes and successful alveolar bone regeneration in rat models indicated this injectable nanocomposite hydrogel provided a promising strategy for the treatment of periodontitis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Guizhou Xu,Zunyao Wang,Lin Sun,Aimin Li,Xianchuan Xie,Xueyuan Gu
Metal-free peracetic acid (PAA) activators are urgently required due to their low toxicity. Heteroatom doping is beneficial to improve the catalytic performance of carbon materials, but the activation performance of doped carbon materials on PAA and the underlying mechanism have not been reported. Herein, nitrogen and sulfur co-doped mesoporous carbons (NS-MC-x, with x representing the mass ratio of thiourea:mesoporous carbon) were fabricated and used as efficient PAA activators for 4-chlorophenol (4-CP) degradation. The NS-MC-1.0/PAA system can remove > 95 % of 4-CP within 10 min, far more than the MC/PAA system. Co-existing anions show no effect on the degradation of 4-CP, whereas humic acid has a certain degree of inhibition. Singlet oxygen ( 1 O 2 ) primarily drives the degradation of 4-CP, with a secondary contribution from a direct electron transfer process mediated by reactive surface complexes, while the contribution of radicals is minimal. In addition, NS-MC-1.0 exhibits excellent reusability for PAA activation, which is conductive to its practical application. This study facilitates further research on metal-free catalysts for PAA activation and offers fresh perspectives on the utilization of PAA in degrading emerging pollutants.
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Adugna Boke Abdeta,Feyisa Wedajo,Qinhan Wu,Dong-Hau Kuo,Ping Li,Hanya Zhang,Ting Huang,Jinguo Lin,Xiaoyun Chen
Cellulose plays a significant role in designing efficient and stable cellulose-based metallic catalysts, owing to its surface functionalities. Its hydroxyl groups are used as anchor sites for the nucleation and growth of metallic nanoparticles and, as a result, improve the stability and catalytic activity. Meanwhile, cellulose is also amenable to surface modifications to be more suitable for incorporating and stabilizing metallic nanoparticles. Herein, the Ag-/Bi-doped Mo(S,O)3 trimetallic sulfo-oxide anchored on B and N codoped cellulose (B–N–C) synthesized by a facile approach showed excellent stability and catalytic activity for PHER at 573.28 μmol/h H2 with 25 mg of catalyst under visible light, and 92.3% of the 4-nitrophenol (4-NP) reduction was achieved within 135 min by in situ-generated protons. In addition to B and N codoping, our use of the calcination method for B–N–C preparation further increases the structural disorders and defects, which act as anchoring sites for Ag-/Bi-doped Mo(S,O)3 nanoparticles. The Ag-/Bi-doped Mo(S,O)3@B–N–C surface active site also stimulates H2O molecule adsorption and activation kinetics and reduces the photogenerated charge carrier’s recombination rate. The Mo4+ → Mo6+ electron hopping transport and the O 2p and Bi 6s orbital overlap facilitate the fast electron transfer by enhancing the electron’s lifetime and photoinduced charge carrier mobility, respectively. In addition to acting as a support, B–N–C provides a highly conductive network that enhances charge transport, and the relocated electron in B–N–C activates the H2O molecule, which enables Ag-/Bi-doped Mo(S,O)3@B–N–C to have appreciable PHER performance.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2012
·Vol. 17
·期 9
·10994-11009
·
IF 2.386
Yunxia Zhang,Yunlan Li,Qingshan Li
The specific aims of this study were to evaluate the inhibition effect on CYP3A of di-n-butyl-di-(4-chlorobenzohydroxamato)tin (IV) (DBDCT), a tin-based complex with high antitumor activity, and the probable mechanism(s) of this action. Adult male SD rats were treated separately with natural saline (NS), lipopolysaccharide (LPS, 5 mg/kg), DBDCT (1.25, 2.5 and 5.0 mg/kg) intraperitoneally for 2 days after induction of CYP3A with dexamethasone (DEX, 100 mg/kg) for 4 days. Western blot analysis and fluorescent quantitation PCR (FQ-PCR) were conducted to determine the changes in expression of CYP3A, PXR, CAR and RXR. The biological accumulation of DBDCT and total Sn were determined by high-performance liquid chromatography (HPLC) and atomic fluorescence spectrometry (AFS). CYP450 content and CYP3A activities were significantly inhibited (p< 0.05) in DBDCT-treated rats compared with the control group, as was the expression of CYP3A (p< 0.05) at both protein and mRNA levels. In DBDCT-treated groups, the expression of PXR protein and mRNA increased, while the expression of CAR decreased. The biological accumulation of DBDCT and Sn in rat livers treated with DBDCT was high. The accumulation of DBDCT and Sn due to the inhibition of CYP3A may be involved in the mechanism of toxicity of DBDCT in rat liver.Keywords:cytochrome P450 3A (CYP3A);di-n-butyl-di-(4-chlorobenzohydroxamato)tin (IV) (DBDCT);inhibition;rat
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Natural Gas Chemistry
·
2012
·
IF 1.348
Lincai Peng,Junping Zhuang,Lu Lin
Effects of Zr/Ti molar ratio in SO 2− 4 /ZrO 2 -TiO 2 solid acid catalyst calcined at different temperatures on its surface properties and catalytic activity were thoroughly investigated in this paper. The physicochemical characteristics of prepared samples were determined by N2 adsorptiondesorption, XRD, NH3-TPD and XPS techniques, respectively. It was found that the crystallization temperature of the samples increased after the combination of ZrO 2 and TiO 2 ; and phase transformations from the anatase to the rutile of TiO 2 species and the tetragonal to the monoclinic of ZrO 2 species were effectively suppressed at higher temperature. The sample with a Zr/Ti molar ratio of 3/1 calcined at 450 °C showed the highest surface area and the most acid sites among all the tested samples. The acid site densities of samples were relatively closed to each other if they were calcined at the same temperature, however, decreased with the calcination temperature. The result indicates that the sulfur content in samples is a crucial factor to control the acid site density. Calcining the sample at 650 °C and higher temperatures resulted in a significant desorption of sulfate ion on the samples. The synthesized samples were evaluated as a potential catalyst for glucose conversion under the near-critical methanol conditions (200 °C/4 MPa). The results suggested that the relatively weaker acid sites of the catalyst were more favorable for the accumulation of methyl glucosides, while the moderate acid sites were responsible for the formation of methyl levulinate. The catalytic activity for methyl levulinate production almost increases linearly with the catalyst acid site density. The catalyst deactivation is due to the loss of sulfate ion and the two catalysts with Zr/Ti molar ratios of 3/1 and 1/3 could effectively alleviate the deactivation caused by sulfate solution in the reaction medium and can be reused after calcination with the reuse rate of over 90% in terms of the methyl levulinate selectivity.
✓ 已验证
产品关联
DOI
高影响因子
Bioengineering & Translational Medicine
·
2024
·
IF 6.1
Juan Deng,Juan Qin,Guolin Song,Chenghai Li,Wentao Tang,Yilin Tang,Xinfang Xiao,Liu Wu,Sicheng He,Yiqing Zhou,Junfen Li,Yan Wang
The potential of low-intensity pulsed ultrasound (LIPUS) in regulating ovarian function has been demonstrated; however, there is a lack of scientific evidence regarding the long-term efficacy of LIPUS in treating ovarian injury and understanding its regulatory mechanisms. In this study, 4-vinylcyclohexene diepoxide (VCD) was used to induce ovarian injury in rats, and LIPUS was applied to target the damaged ovarian tissues. The research aimed to investigate the long-term protective effect of LIPUS against ovum toxicity induced by VCD and elucidate the associated molecular mechanisms. During the experiment, HE staining was employed for observing the morphology and structure of the ovary, while protein sequencing was utilized for identifying and confirming the molecular mechanism through which LIPUS restores the damaged ovarian structure. The long-term effectiveness of LIPUS in protecting against ovarian injury was evaluated through ELISA, estrous cycle monitoring, fertility testing, and behavioral analysis. The results indicated that LIPUS effectively restored the structure of damaged ovaries. Both in vivo and in vitro studies revealed that this protective effect may be attributed to LIPUS inhibiting apoptosis of ovarian granulosa cells (GCs) by regulating Daxx-mediated ASK1/JNK signaling pathway. Subsequent functional tests demonstrated significant improvements in sex hormone secretion and regulation of estrous cycle within 6 cycles following LIPUS treatment. Additionally, there was a notable increase in offspring numbers after mating. Behavioral analysis revealed that LIPUS effectively alleviated menopausal symptoms resulting from ovarian injury including mood fluctuations, cognitive behavior changes, and reduced muscle excitability levels. These findings suggest that beneficial effects of LIPUS may help reduce VCD-induced ovarian damage with long-term efficacy.
✓ 已验证
产品关联
DOI
高影响因子
MARINE ENVIRONMENTAL RESEARCH
·
2025
·
IF 3.2
Jingwei Wang,Jiaxin Wang,Pan Zhao,Shuzhen Li,Xuwang Zhang,Dan Xu,Qiao Ma
2-Methyl-4-isothiazoline-3-one (MIT) is a widely employed antimicrobial agent frequently detected in environments; however, current documentation regarding its impacts on microbial communities and biodegradation processes remains limited. Herein, marine sediment systems were established to investigate the MIT impacts under tiered concentrations (CK, 0 μg/L; LC, 5 μg/L; MC, 50 μg/L; HC 5 mg/L). The extracellular polymeric substance contents increased in the LC and MC groups, but not in the HC group, after one day of exposure. MIT inhibited the protease, dehydrogenase, and catalase activities in the HC group after 14 days of exposure. Bacterial community structure was altered in response to high concentrations of MIT. Lutibacter (44.59 %) and Flavobacterium (7.90 %) were the predominant genera that maintained its stability. Cognatiyoonia and Pseudorhodobacter decreased on Day 1, while Rhodoferax and Pseudorhodobacter increased on Day 14. Network analysis demonstrated reduced bacterial community interactions. Functional prediction indicated that MIT inhibited carbon metabolism and citrate cycle process in the HC group. These results suggested the potential ecotoxicological risks of MIT to marine systems under prolonged exposure conditions. Furthermore, three novel bacterial strains, i.e., Brevibacterium sp., Microbacterium sp., and Epilithonimonas sp., were isolated with demonstrated MIT biodegradation capacity for the first time. This study advances our comprehension of the impacts of MIT on the marine environment and provides valuable bacterial resources for bioremediation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2024
·Vol. 12
·期 8
·2764-2771
·
IF 5.7
Xiao-Feng Wang,Chen-Yan Xu,Rui-Lian Lin,Wen-Qi Sun,Ming-Fu Ye,Li-Xin Xu,Jing-Xin Liu
Multistimuli-responsive materials are highly desirable due to their convenient controllability and potential applications in smart window, sensor and bionic manufacturing. Herein, we designed and synthesized a guest molecule 1-[2-(4-fluorophenyl)-2-oxoethyl]-4,4′-bipyridinium dichloride (FOV·Cl2), whose ketone–enol tautomerism (FOV2+ ↔ FHV+) in aqueous solution is governed by pH values. The solid-state inclusion complex formed β-cyclodextrin (β-CD) and FOV2+ (in the ketone form) demonstrated multiple chromic behaviors under the external stimuli of light, heat and vapors of ammonia and some organic amines, which were characterized by UV-vis and ESR spectroscopy. For comparison, the chromic behavior of the solid-state inclusion complex of β-CD with FHV+ (in the enol form) was also investigated. In addition, we found that the fluorescence emission of the inclusion complex FOV2+@β-CD can be reversibly modulated by the photochromic process. The chromic and fluorescent properties of the FOV2+@β-CD endow it with practical utility in inkless printing, ammonia sensing and multiple anti-counterfeiting. This work focused on demonstrating the multistimuli-responsive chromic behaviors of the inclusion complexes and exploring their promising applications.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）