BIOTECHNOLOGY LETTERS
·
2025
·Vol. 48
·期 1
·2
·
IF 2.1
Yang Xiaole,Tian Xia,Zhu Hai,Jia Weiwei,Li Jiahuan,Tang Ruizhou,Zhang Chuanzhi,Cao Yang,Li Tingting
Chiral amines, as essential chiral building blocks in drug synthesis, present a considerable challenge in biomanufacturing due to the requirement for highly efficient stereoselective synthesis. In this study, we successfully cloned and heterologously expressed a novel ( R )-amine transaminase, MagAT, from Mycolicibacterium agri . Systematic analysis showed optimal activity at pH 7.0, with the highest reaction rate occurring within 30 min at 50 ℃. However, considering overall thermal stability, 40℃ was selected as the operating temperature for subsequent experiments. Furthermore, the enzyme retained nearly 100% catalytic activity in the presence of 10% methanol, DMSO, and chloroform. Kinetic analysis demonstrated that MagAT possessed high substrate affinity, with Michaelis constants (K m ) of 5.13 ± 0.45 mM for ( R )-1-phenylethan-1-amine and 3.73 ± 0.34 mM for pyruvate. Substrate specificity studies indicated that MagAT efficiently catalyzed the conversion of C3-C8 aliphatic amines (e.g., ( R )-2-aminooctane) and monocyclic aromatic amines (e.g., ( R )-1-phenylethan-1-amine), whereas its catalytic activity significantly declined for sterically hindered substrates such as naphthylamine. 3D structural modeling with AlphaFold3 and molecular docking showed that steric hindrance from key residues in the O-pocket critically influences substrate specificity. This study not only provides an efficient biocatalyst for the biosynthesis of chiral amines but also establishes a theoretical basis for the rational design of transaminases with broader substrate adaptability.
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Nature Communications
·
2025
·Vol. 16
·期 1
·10578
·
IF 15.7
Li Shiyun,Liu Guangsheng,Liu Chuhao,Fu Yifan,Fu Yixuan,Xu Yifei,Li Chengyu,Zhang Xueqiang,Xu Bingjun,Li Wan-Lu,Li Mufan
Electrocatalysis provides a sustainable alternative route to produce nitrogen-containing molecules. However, poor carbon-nitrogen (C-N) coupling selectivity and limited current density pose challenges to its widespread adoption. Herein, we introduce a carbon-defect enhanced 2,2,6,6-tetramethylpiperidine N-oxyl (TEMPO) mediated tandem process to tackle both problems. Our hetero-homogeneous system achieves a Faraday efficiency of ~99% with industrial-level current density of ~0.6 A·cm −2 for urotropine electrosynthesis. In situ near ambient pressure X-ray photoelectron spectroscopy and quasi in situ electron paramagnetic resonance reveal that the boosted activity originated from the oxidation of TEMPOH on the carbon defective sites, which accelerates the redox cycling of the molecular mediator for urotropine formation. This work highlights the catalytic effect of carbon defects on the redox cycling of TEMPO, improves both the selectivity and the rate of the electrocatalytic C-N coupling reaction, and offers insights for designing efficient electrochemical mediated oxidation processes and C-N coupling reactions.
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2025
·
IF 3.1
Pan Xuhai,Liu Yuqi,Zhao Xinyi,Yu Andong,Hua Min,Jiang Juncheng
Benzoyl peroxide (BPO) is a thermally sensitive organic peroxide prone to thermal runaway under incompatible conditions. This study investigates the thermal decomposition behavior of BPO and its mixtures with phenyl-containing compounds (toluene, benzene, biphenyl, p-terphenyl) using differential scanning and adiabatic calorimetry. Apparent activation energies were calculated using Starink and Friedman methods across a conversion range of 0.1–0.9. Results show that the addition of phenyl compounds lowers both the initial decomposition temperature and activation energy of BPO, with benzene having the most significant effect. Adiabatic analysis revealed two distinct decomposition stages, low- and high-temperature regions, characterized by different kinetic behaviors. The early stage showed higher activation energy and stronger autocatalytic features. Overall, the presence of phenyl-containing substances reduces the energy barrier and thermal runaway threshold of BPO systems, increasing their thermal reactivity and hazard. These findings provide a reference for safety assessment and hazard control in the storage and handling of BPO and related materials.
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MRS Communications
·
2025
·
IF 2.3
Hua Lingwen,Zhang Zhuoqing,Cao Lei,Li Huijie,Yang Rui,Zheng Weilin,Zhang Xing
Zirconium-doped SiOC (SiZrOC) ceramics with different Zr/Si molar ratios were fabricated through a thiol-ene cross-linking reaction followed by pyrolysis for high-temperature thermal insulation. The zirconium could be incorporated into the amorphous SiOC network when the Zr/Si molar ratio was below 0.25. The addition of zirconium can impede further decomposition of amorphous SiZrOC network, thereby significantly enhancing the high-temperature stability (above 1400°C). The SiZrOC-0.25 ceramics with octet truss structures were fabricated by digital light processing followed by pyrolysis at 1000°C, which exhibited a compressive strength of 6.42 ± 1.74 MPa even after heat treatment at 1400°C for 6 h under air atmosphere. The back temperature of the SiZrOC-0.25 ceramic was 205°C after exposure to 800°C for 20 min. Thus, the SiZrOC ceramics developed in this work are likely suitable for high-temperature thermal insulation, particularly in aerobic environments. Graphical abstract
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CELL DEATH AND DIFFERENTIATION
·
2025
·
IF 15.4
Yan Liping,Zhu Wenjie,Wang Mengqi,Zhao Ruinan,Zhang Guohao,Guo Xiangyu,Li Chunlan,Wang Suxia,Zhang Hui,Gao Peng
The role of autophagy in gastric cancer (GC) progression remains elusive, warranting further investigation into its mechanisms and therapeutic implications. In this study, we demonstrate that olfactory receptor family 2 subfamily T member 6 (OR2T6) is downregulated and correlates with poorer prognosis in GC tissues. Functionally, OR2T6 induces autophagy initiation while blocking autophagic flux by compromising lysosomal function and inhibits cell proliferation both in vitro and in vivo. Mechanistically, co-immunoprecipitation (Co-IP) and mass spectrometry (MS) analyses reveal that OR2T6 specifically binds to PPP3CA. OR2T6 facilitates the protein stability and enzyme activity of PPP3CA through the ubiquitin-proteasome system and the promotion of calcium ion influx via the Gs/cAMP/PKA channel signaling axis. Our further research demonstrates that OR2T6 binds to PPP3CA, which suppresses the AKT/mTOR signaling pathway, thereby inhibiting tumor proliferation and promoting autophagy initiation. Interestingly, it facilitates the nuclear translocation of TFEB, a key regulator of lysosomal biogenesis, which leads to the transcriptional inactivation of lysosomal target genes (LAMP1, MCOLN1, ATP6V1H, CTSB, and CTSD), impairing lysosomal function and blocking autophagic flux. Collectively, we report that OR2T6 binds to and promotes PPP3CA, which subsequently initiates autophagy, inhibits proliferation by suppressing the AKT/mTOR pathway, and then blocks autophagic flux through TFEB-mediated lysosomal dysfunction. These findings suggest that OR2T6 may be a potential therapeutic target for GC.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 12
·830
·
IF 5.3
Hou Hongming,Long Jiajun,Li Xianglong
The chelation interaction between sodium lignosulfonate (SLS) and Cu 2+ is exploited to construct an enzyme-free creatinine sensing electrode (SLS-Cu/SPCE) via one-step electrodeposition on screen-printed carbon electrodes (SPCE). SLS chelation moderates Cu 2+ electro-reduction kinetics, resulting in finely structured Cu particles with a uniform and dense distribution. These Cu particles, with abundant unsaturated coordination sites, enhance creatinine adsorption and promote specific complexation with Cu 2+ , thereby increasing the oxidation current response for sensitive detection. The SLS-Cu/SPCE achieved a 0.12 µM detection limit, a 10–300 µM linear range, high reproducibility, and long-term stability over 28 days. Tests in human urine samples showed good agreement with enzymatic assays (relative error < 16%). The SLS-Cu 2+ coordination strategy offers a novel approach for optimizing Cu-based enzyme-free electrochemical creatinine sensors. Graphical A sodium lignosulfonate-Cu 2+ chelation strategy produces refined copper particles on SPCE with abundant unsaturated sites. These sites enhance creatinine adsorption and Cu 2+ complexation, enabling highly sensitive non-enzymatic detection (0.12 μM LOD, 10-300 μM range) with strong anti-interference and excellent stability.
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Nature Communications
·
2025
·Vol. 16
·期 1
·10365
·
IF 15.7
Qin Mengmei,He Meng,Cheng Chuanqi,Li Rui,Liu Cuibo,Zhang Bin
Electrocatalytic deuteron-dechlorination of trichloroacetic acid (TCAA) in D 2 O provides a green route to synthesize commercial acetic- d 3 acid- d (AA- d 4 ). Synthesizing AA- d 4 with a high Faradaic efficiency (FE) and reaction rate is highly challenging because of the difficult C−Cl bond deuteration of the 2-monochloroacetic-2,2- d 2 acid- d (MCAA- d 3 ) intermediate. Here, a quaternary ammonium salt surfactant-modified low-coordination copper electrocatalyst is designed, achieving TCAA-to-AA- d 4 with a 91% selectivity, 91% FE and 0.59 mmol h −1 reaction rate at −100 mA cm −2 . Mechanistic and kinetic studies reveal that the surfactant enhances the adsorption of MCAA- d 3 through electrostatic forces and increases the electron deficiency of Cu δ+ sites, which accelerates electron transfer and promotes C−Cl bond activation, increasing the AA- d 4 selectivity. Surfactant-induced low D 2 O coverage suppresses D 2 formation, improving the FE. AA- d 4 electrosynthesis (1.84 g) with a 30 mmol h −1 reaction rate and 65% FE at 600 mA cm −2 and deuterated drug applications demonstrate promising potential.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 12
·832
·
IF 5.3
Li Jiejun,Zhao Yixian,Huang Jiaxin,Wang Jiamin,Zhang Rongxia,Ni Meijun,Zhao Pengcheng,Fei Junjie,Xie Yixi
A highly sensitive electrochemical sensor for chlorogenic acid (CGA) detection has been developed using nitrogen-doped carbon nanorods loaded with NiCo bimetallic (NCNRs/NiCo). The composite material was synthesized through a pyrolysis strategy where NiCo-layered double hydroxide (LDH) was grown in situ on phenolic resin-derived carbon nanorods and subsequently carbonized. Material characterization confirmed the successful formation of uniformly dispersed NiCo nanoparticles anchored on nitrogen-doped carbon matrix, which provided abundant active sites. The NCNRs/NiCo-modified glassy carbon electrode (GCE) exhibited exceptional electrocatalytic activity toward CGA oxidation, achieving a wide linear detection range from 0.5 nM to 10 µM with an ultralow detection limit of 0.32 nM (S/ N = 3). The sensor demonstrated excellent selectivity against common interferents, good reproducibility and repeatability. Practical application in food samples showed satisfactory recoveries (97.6-104.1%) in good agreement with the ultraviolet-visible (UV-Vis) reference method. The outstanding performance originates from the synergistic effects between nitrogen-doped carbon framework and bimetallic nanoparticles, offering a promising platform for natural antioxidant determination in food quality control. Graphical
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-13
·
IF 3.9
Zhang Yang,Huang Yu-Han,Wang Hou-Jie,Zheng Lin-Guo,Yang Qi-Yu,Wang Yan-Ru,Zhao Jiang,Jiang Cheng-Lu
This study primarily focuses on utilizing hair strands to achieve the crystallization of lithium carbonate, aiming to investigate the factors influencing crystal formation and volume variation by altering external conditions. To research the reason of crystallization, we have identified the following influencing factors: the direction of crystal growth, the temperature, the evaporation rate, selection of containers. The study ultimately revealed that the container material, solution temperature, evaporation rate, and crystal growth surface all influence crystallization and crystal volume.
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Science China-Materials
·
2025
·
IF 7.4
Zhang Na,Xu Ziwei,Yang Zhou,Zhang Lu,Fan Zihao,Xu Dongfang,Wang Fei,Yuan Ningyi,Ding Jianning,Cui Jian,Liu Zhike
Inorganic perovskite solar cells (IPSCs) have garnered significant research interest in recent years owing to their excellent light and thermal stability, as well as their potential applications in tandem solar cells. However, the power conversion efficiency (PCE) and stability of IPSCs are often compromised by residual lattice stress and numerous defects at interfaces and within the bulk material, which lead to severe nonradiative recombination of photogenerated carriers. To address these challenges, we developed a zero-dimensional supramolecular complex, (ETP) 2 SbCl 5 , serving as both a dual interface and bulk modifier to regulate the growth of inorganic CsPbI 3 perovskite films. The (ETP) 2 SbCl 5 modifier exhibits a unique spatial distribution: the ETP + cations preferentially anchor at the buried interface, passivating defects on both TiO 2 and perovskite surfaces, while Sb 3+ and Cl − ions diffuse into the perovskite bulk during annealing, effectively relieving residual lattice stress. Moreover, Cl − anions accumulate on the top surface of the CsPbI 3 film, passivating cation defects. Consequently, the (ETP) 2 SbCl 5 -modified CsPbI 3 IPSC achieves a high-quality active layer with significantly reduced defects and suppressed energy loss, an impressive PCE of 21.71% and a high open circuit voltage of 1.27 V, remaining 97.4% of their initial efficiency after 500 h of continuous maximum power point (MPP) tracking.
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JOURNAL OF MATERIALS SCIENCE
·
2025
·
IF 3.9
Wang Qing,Yu Mingguang,Chen Guangxue,Chen Zijian,Zhong Yingying,Mo Qijie,Wang Honghu
Herein, a facile strategy for fabricating cellulose-based paper with dual humidity-pH responsive room-temperature phosphorescence (RTP) through molecular excited-state modulation was reported. The phosphorescent system was constructed by immobilizing ethylenediamine-mediated condensation product of 4-bromo-1,8-naphthalic anhydride (forming 1,8-naphthalimide derivatives) onto cellulose matrix via hydrogen-bond rigidification. Post-papermaking and drying processes, the developed material exhibits remarkable excitation-dependent dual emission: orange-red phosphorescence ( τ = 6.3 ms) under ambient conditions and blue fluorescence ( τ = 3.3 ns) under saturated humidity. Notably, the humidity-responsive luminescence switching demonstrates excellent reversibility after drying cycles. Furthermore, the incorporated naphthalimide moiety preserves its inherent pH sensitivity, enabling simultaneous pH detection through emission wavelength shifts. Systematic characterization reveals that the humidity response originates from water-molecule regulated intersystem crossing efficiency, while the pH response stems from protonation-induced electronic structure modifications. This work establishes a novel paradigm for designing multi-stimuli responsive smart materials through rational manipulation of molecular excited states, demonstrating potential applications in paper-based anti-counterfeiting technologies.
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JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2025
·Vol. 40
·期 6
·1563-1571
·
IF 1.1
Cao Wenjing,Liu Yiming,Zhao Dongmei,Wan Xiaobo,Liu Yang,Huang Kun,Shi Minxian
A Ti 3 SiC 2 -modified high-silica oxygen/phenolic aerogel composite with excellent oxidation resistance and high-temperature performance was prepared. The experimental results show that the obtained composite has significantly improved oxidation resistance. When the addition amount of Ti 3 SiC 2 is 75%, the carbonization volume shrinkage rate of the composite after aerobic static combustion is only 5.95%. At the same time, the LAR and MAR after 30 seconds of oxyacetylene ablation under a heat flux density of 1.5 MW/m 2 are 0.0307 mm/s and 0.0149 g/s, respectively. The compressive strength after aerobic static combustion at 1 000 °C is up to 20.43% of that before aerobic static combustion, which is 1.99 times that of the unfilled material, significantly improving the high-temperature mechanical properties of the composite.
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