FOOD CHEMISTRY
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2024
·
IF 8.5
Chao Zhang,Chunhe Gu,Mengrui Wang,Junxia Chen,Haode Chang,Ziqing Chang,Junping Zhou,Mingzhe Yue,Wenjing Zhang,Quanmiao Zhang,Zhen Feng
Noni fruit has an unpleasant flavour but is highly bioactive. Therefore, it is necessary to clarify the effect of temperature regulation on quality of fermented noni fruit. In the present study, the formation of flavours, amino acid profiles, and iridoid glycosides during noni fruit fermentation at different temperatures were investigated. We initially found that different temperatures affected core microbial communities. The general evolutionary trends of Acetobacter and Gluconobacter were influenced by different temperatures. Furthermore, high temperature helped maintain low octanoic and hexanoic acids. Subsequently, we found that high temperature improved total amino acids and iridoid glycosides. The correlation network analysis revealed that bacterial communities impacted the quality (volatile flavours, amino acid profiles, and iridoid glycosides) of fermented noni fruit. Overall, altering the temperature induced variations in microbial communities and quality during the noni fruit fermentation process. These results are instrumental in the pursuit of quality control in natural fermentation processes.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Wissam Bessa,Djalal Trache,Mehdi Derradji,Houda Ambar,Ahmed Fouzi Tarchoun,Mokhtar Benziane,Bendiba Guedouar
In the present work, giant reed cellulosic fibers ( Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a). RF were extracted from giant reed cane, and subjected to different chemical treatments using either alkaline, silane or their combining treatments. The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques. Broadly, the morphology of the treated fibers is affected, their crystallinity and thermal stability increased. The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods. A decrease in the heat of curing as well as activation energy ( Ea ) was reported. The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
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Toxics
·
2025
·Vol. 13
·期 6
·427
·
IF 4.1
Xinyi Xie,Jiayin Hou,Meng Li,Zhiyu Liu,Mengai He,Chenxi Li,Xiaohua Du,Liezhong Chen
Phoxim is a pesticide extensively applied in mulberry fields, and residues may persist on leaves even after the recommended pre-harvest interval. However, the potential risks of these residues toBombyx moriL. (Lepidoptera: Bombycidae) have long been overlooked. The results demonstrated that chronic low-dose exposure from the second to fifth instars significantly impaired silkworm development and silk production. Specifically, larvae in the 0.316 μg/mL treatment group (1/2 LC50) exhibited a significant reduction in body weight, while the cocoon shell ratio was significantly decreased in both the 0.079 μg/mL (1/8 LC50) and 1/2 LC50groups. Cocoon deformities were observed in the 0.032 μg/mL (1/20 LC50), 1/8 LC50, and 1/2 LC50groups. Histopathological analysis revealed silk gland damage in the treatment groups, with severity increasing with higher phoxim concentrations. Biochemical analyses indicated elevated malondialdehyde (MDA) levels accompanied by increased superoxide dismutase (SOD) and peroxidase (POD) activities. Notably, phoxim exposure selectively reduced juvenile hormone (JH) titers without affecting ecdysone titers. JH-regulated genes including the receptorsMet1andMet2, and transcription factorsKr-h1andDimmwere downregulated, accompanied by suppressed expression of the fibroin synthesis geneFib-H. These results collectively indicate that chronic low-concentration phoxim exposure disrupts endocrine regulation, damages silk gland integrity, and ultimately reduces silk production in silkworm.
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ENVIRONMENTAL POLLUTION
·
2025
·
IF 7.3
Chao Huang,Yilin Liu,Jing Lu,Xunzhi Deng,Zhoubin Liu,David P. Molloy,Langtao Xiao,Zhoufei Luo
Bisphenol A (BPA), an emerging endocrine-disrupting chemical found in irrigation water, soil, and agricultural plastic mulch, presents potential exposure risks to pepper plants ( Capsicum annuum L.). This study investigated BPA impact, observing that BPA accumulates in a concentration-dependent manner, with highest levels in roots and lowest in fruit. High BPA exposure decreased photosynthetic pigment content, reduced stomatal size, caused chloroplast dilation, and increased reactive oxygen species and malondialdehyde accumulation, disrupting hormone balance in leaves with a 2.3-fold increase in abscisic acid content under 100 mg/kg BPA treatment. Integrated multi-omics analyses revealed that BPA toxicity operates through interference with galactose metabolism, starch-sucrose metabolism, plant hormone signaling, and glutathione metabolism pathways. Key molecular responses included significant downregulation of photosynthesis-related genes and substantial metabolic perturbations, notably an 8.1-fold reduction in uridine diphosphate fructose and 10.6-fold accumulation of indole-3-ethanol. By employing an integrated multi-omics approach, this study provides a comprehensive mechanistic understanding of BPA phytotoxicity that extends beyond conventional physiological observations. The findings offer novel insights into the molecular regulatory networks underlying plant stress responses to endocrine-disrupting chemicals.
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JOURNAL OF CHROMATOGRAPHY A
·
2014
·
IF 4.258
Wenhua Ji,Xiuli Ma,Hongkai Xie,Lingxiao Chen,Xiao Wang,Hengqiang Zhao,Luqi Huang
Dummy molecularly imprinted polymers (DMIPs) for simultaneously selective removal and enrichment of ginkgolic acids (GAs) during the processing of Ginkgo biloba leaves have been prepared. Two dummy template molecule with similar structural skeleton to GAs, 6-methoxysalicylic acid (MOSA, DT-1) and 6-hexadecyloxysalicylic acid (HOSA, DT-2), have been designed and synthesized. The performance of the DMIPs and NIPs were evaluated including selective recognition capacity, adsorption isotherm, and adsorption kinetics. The selective recognition capacity of the three GAs with four analogues on the sorbents illustrated that the DMIPs sorbents have high specificity for GAs. An efficient method based on DMIP-HOSA coupled with solid-phase extraction (SPE) was developed for simultaneously selective removal and enrichment of ginkgolic acids (GAs) during the processing of Ginkgo biloba leaves. The method showed excellent recoveries (82.5–88.7%) and precision (RSD 0.5–2.6%, n = 5) for licorice extracts, Gastrodia elata extracts and pepper extracts spiked at three concentration levels each (50, 100, 200 μg mL −1 ). The results indicated that GAs and standardized Ginkgo biloba leaves extracts could be obtained simultaneously through the DMIP-SPE.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Kok Bing Tan,Meixia Zheng,Junyan Lin,Yujing Zhu,Guowu Zhan,Jianfu Chen
Pullulan-based composite film can be a potential alternative packing material to non-environmentally friendly plastic wrap (PE) to preserve fresh-cut carrots. However, many developed pullulan-based composites either have high water vapor permeability (WVP) and high mechanical strength or vice versa, which limits the practicality of the developed packaging materials for potential commercialization. Herein, Abelmoschus manihot gum (AMG)/pullulan/magnesium L-ascorbate (MLA) was created as a green composite film (APL) to preserve fresh-cut carrots. The optimal amount of MLA was found to be 10 % (APL10), demonstrating a balance of lower WVP and greater mechanical strength and antioxidant performance than many pullulan-based films. This effectively solved many problems faced by other pullulan-based packaging films. After the fresh-cut carrots were packed with the composite film for 4 days, it was found that APL10 was effective in preserving the quality of carrots, in terms of freshness, weight loss rate, Vitamin C (VC), and malondialdehyde (MDA) content after 4 days of storage, much better than non-biodegradable PE. Thus, based on these findings, it is concluded that APL films have huge potential as a green packaging material for food to replace PE in the future.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Siqi Peng,Jing Wang,Mohamed A. Farag,Mahmoud Salah,Lianliang Liu,Yajing Fang,Weimin Zhang
This study investigated the changes in phytochemical composition and inflammatory response of crude papaya ( Carica papaya L.) seed oil (CPO) and its refined forms (degummed, PDG; deacidified, PDA; decolorized, PDC; deodorized, PDO). Oils were analyzed for their phytochemical composition, oil quality parameters, antioxidant activity , and their inflammatory response in LPS-stimulated THP-1 macrophages. At higher refining degrees, particularly after deacidification , the contents of phytochemicals (sterols, tocopherols , and polyphenols) decreased while oxidation products increased. Both CPO (0.1–1.0 mg/mL) and PDG reduced the secretion and mRNA expression of LPS-stimulated inflammatory cytokines and mediators and also blocked the activation of the NF-κB pathway. PDA, PDC, and PDO showed low anti-inflammatory or even pro-inflammatory activity. Correlation analysis showed that 4 polyphenols and 2 phytosterols were responsible for the oil's anti-inflammatory effects. These findings indicated that moderate refining is suggested for papaya seed oil processing for retaining bioactive ingredients and anti-inflammatory ability.
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2025
·
IF 5.7
Qiaoran Ma,Guobing Tian,Shengchao Yang,Junwen Chen,Wei Fan,Ping Zhao,Yan Wang,Jiamin Liu,Yonglin Liu,Shuhui Zi,Shuran He
Planting vegetation under forests in agroforestry systems fosters sustainable agricultural development. However, Limited availability of biostimulants for agroforestry and unclear mechanisms of plant growth promotion. This study synthesized and evaluated a novel biostimulant, nanosilicon-based vermicompost leachate (NSVCL), using Panax quinquefolius L. as the research plant species for forest cultivation. Trichoderma harzianum (TH) was chosen to represent a biostimulant with broad-spectrum properties, and its application effects were compared with NSVCL. The regulatory effects of both on the physiological characteristics and rhizosphere soil microenvironment of P. quinquefolius were investigated, with untreated plants serving as controls. Compared to the control, NSVCL and TH increased the dry weight of P. quinquefolius roots 129.33 % and 23.50 %, respectively. NSVCL was applied more effectively than TH. Additionally, NSVCL markedly improved leaf anatomical traits, including palisade and spongy tissue thickness, overall leaf thickness, chloroplast number, and cuticle thickness. Application of NSVCL and TH significantly elevated the net photosynthetic rate (Pn) by 86.55 % and 60.92 %, respectively, and increased total chlorophyll content (TChl) by 24.91 % and 11.76 %. Biostimulants facilitated nutrient uptake and boosted antioxidant enzyme activity in P. quinquefolius . Partial least squares path modeling (PLS-PM) further demonstrated that both NSVCL and TH promoted plant growth by enhancing soil enzyme activity in forest environments. These findings underscore NSVCL's efficacy in improving P. quinquefolius growth under forest conditions and provide a practical foundation for advancing organic forest cultivation and sustainable forest-medicine integration.
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Analytical Methods
·
2015
·Vol. 7
·期 6
·2855-2861
·
IF 1.821
Zhuo Ye,Lingxi Yang,Jianguo Wen,Baoxian Ye
A novel voltammetric sensor, based on a single-walled carbon nanotube (SWNT) composite poly(L-serine) film modified electrode, was fabricated by simple dipping–drying and electrodeposition methods and used for highly sensitive determination of natamycin. At this sensor, the electrochemical response of natamycin was improved greatly due to the synergistic effect of SWNTs and poly(L-serine). A new voltammetric method for the determination of natamycin was proposed with a detection linear range of 6 × 10−8 mol L−1 to 6 × 10−6 mol L−1 and a detection limit of 4 × 10−8 mol L−1. Furthermore, the electrode reaction of natamycin was investigated in detail and some dynamic parameters were calculated for the first time. Using the present method, the residual quantity of natamycin in available claret and beverage samples was evaluated with good satisfactory recovery.
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MOLECULES
·
2025
·Vol. 30
·期 5
·998
·
IF 4.6
Yuanqi Duan,Jinfeng Sun,Yongkang Xue,Weiwei Xu,Yuxin Jiang,Tieqiang Zong,Wei Zhou,Zhengyu Hu,Gao Li
Given that the preparation method of polysaccharides affects the functional properties, four types of acidic polysaccharides (BCP30-1a, BCP50-1a, BCP70-1a, and BCP90-1a) were prepared using the gradient ethanol precipitation method. Then, a series of chemical and instrumental analysis techniques were used to compare structural characteristics and morphology. Neuroprotective effects were explored using OGD/R-induced PC12 cells. The results showed that BCP30-1a, BCP50-1a, BCP70-1a, and BCP90-1a had similar characteristic groups and contained both β-glycosidic and α-glycosidic bonds. Their molecular weights, in descending order, were 198.398 kDa, 184.690 kDa, 184.556 kDa, and 184.217 kDa, respectively. In addition, the four polysaccharides contained different proportions of glycosidic bonds, namely, Manp-(1→, →5)-Araf-(1→, →3)-Galp(or GalAp)-(1→, →4)-Glcp-(1→ and →3,6)-Galp-(1→. BCP30-1a also contained a certain proportion of Galp-(1→, and each polysaccharide had different microscopic characteristics and good thermal stability. Finally, BCP50-1a, BCP70-1a, and BCP90-1a exhibited good cytoprotective effects on PC12 cells based on the OGD/R model. These findings provide a novel regulatory strategy for the functional activity of BCPs and offer scientific evidence supporting application in the research field of ischemic stroke.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Yangzhou Xie,Sitao Wang,Yu Tian,Kexi Ye,Zhigang Xu,Han Zheng,Wei Jiang,Fengmei Zhang,Xiaoxi Si
Excessive D-lactic acid in fermented foods can result in unpleasant flavors and is difficult for the human body to metabolize. This study is the first to combine tandem column technology (C18 and Poroshell chiral-T) with a machine learning strategy. The method achieved a resolution of 2.86, with a concentration range of 50.00 μg·L −1 -50.00 mg·L −1 and a limit of detection of 16.67 μg·L −1 . We aimed to classify different lactic acid products and tobacco, and the research showed that L-lactic acid was predominant in all food analyses , while D-lactic acid was only found in lactic acid products In addition, the machine learning algorithms were optimized, and the random forest model effectively distinguished samples with similar lactic acid content. Based on this collaborative model of tandem column and machine learning, lactic acid chiral biomarkers gained the ability to classify food samples. The accuracy of model reached 80 %. This strategy contributes to the chiral analysis of lactic acid in complex food matrices. It also demonstrates the practical value of classification in food research.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
·
IF 6.8
Hua Huang,Genlin Mao,Dongjie Liu,Shenghua Ding
The cuticle layer covering on surface plays important roles in the quality changes including visual of appearance, texture, and water loss for fruit. The surface microstructures, chemical composition of epi- and intracuticular waxes, and cutin monomers, as well as their effects on fruit transpiration in Sanhua plum were comprehensively carried out. Microscopic observation revealed an irregular surface morphology characterized by continuous crusts interspersed with sparsely distributed nano-granular crystals. Chemical analysis demonstrated that the epicuticular waxes were primarily composed of very-long-chain n -alkanes, secondary alcohols, aldehydes, fatty acids, primary alcohols, and alkyl esters. The intracuticular waxes contained comparable levels of very-long-chain components and pentacyclic triterpenoids. The cutin monomers were predominantly C 16 -dihydroxy fatty acids, and other hydroxyl fatty acid derivatives, present with a moderate level as other reported Rosaceae family fruit. Compared to cutin polymers, the cuticular waxes contributed approximately 76.9 % of the total barrier to water loss, with the epicuticular wax layer exhibiting 1.6-fold to the intracuticular wax layer for the barrier efficiency in Sanhua plum fruit. The elucidation of the potential effect of surface lipids on transpiration was likely associated with the structural and chemical distribution of cuticular wax layers within the cuticle.
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危险品化学品经营许可证（带存储）